FastQCFastQC Report
Fri 27 May 2016
SRR2139340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23061945
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA296600.050.5734861
GTATCAA539750.036.3134461
TCAACGC684750.028.048924
ATCAACG690100.027.8374793
CAACGCA698800.027.4791565
AACGCAG716150.026.9234376
TATCAAC718500.026.9210532
ACGCAGA826200.023.2719867
CGCAGAG840300.022.8864218
GTACATG751950.020.9862581
TACATGG746200.020.586032
ACATGGG757050.019.9587543
GCAGAGT970000.019.6427759
GTGGTAT133550.019.2119141
AGAGTAC882400.018.43273510-11
GAGTACT561700.018.3970112-13
CAGAGTA952750.017.77021810-11
CATGGGG563950.017.5432264
TGGTATC134300.017.4662042
AGTACTT611100.016.66894712-13
Produced by FastQC (version 0.11.5)