##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23061945
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.633691694260825	31.0	31.0	34.0	30.0	34.0
2	31.72215175259502	31.0	31.0	34.0	30.0	34.0
3	31.803959466558435	31.0	31.0	34.0	30.0	34.0
4	35.5726555153956	37.0	35.0	37.0	33.0	37.0
5	35.428212668098894	37.0	35.0	37.0	33.0	37.0
6	35.4567218419782	37.0	35.0	37.0	33.0	37.0
7	35.361632594302	37.0	35.0	37.0	32.0	37.0
8	35.33555018885007	37.0	35.0	37.0	32.0	37.0
9	36.93905327586203	39.0	37.0	39.0	33.0	39.0
10-11	36.83057471952171	39.0	37.0	39.0	32.0	39.0
12-13	36.78533011851342	39.0	37.0	39.0	32.0	39.0
14-15	37.907555108643265	40.0	37.0	41.0	32.5	41.0
16-17	37.86720298309618	40.0	37.0	41.0	32.0	41.0
18-19	37.889395625564106	40.0	37.0	41.0	32.0	41.0
20-21	37.85356164349136	40.0	37.0	41.0	32.0	41.0
22-23	37.77128483397576	40.0	37.0	41.0	32.0	41.0
24-25	37.66508897942205	39.5	36.5	41.0	32.0	41.0
26-27	37.443595275553406	39.0	36.0	41.0	31.5	41.0
28-29	37.29539477387949	39.0	36.0	41.0	31.0	41.0
30-31	37.116576588249615	39.0	36.0	40.5	31.0	41.0
32-33	36.921833633909486	39.0	35.5	40.0	30.0	41.0
34-35	36.77397587193562	38.5	35.0	40.0	30.0	41.0
36-37	36.60157287537278	38.0	35.0	40.0	30.0	41.0
38-39	36.435671521243606	38.0	35.0	40.0	30.0	41.0
40-41	36.29316511408014	38.0	35.0	40.0	30.0	41.0
42-43	36.09107159464036	38.0	34.5	40.0	29.5	41.0
44-45	36.17403249628569	38.0	34.5	40.0	29.5	41.0
46-47	36.640494756587074	39.0	35.0	40.0	30.0	41.0
48-49	36.73491092640489	39.0	35.0	40.0	30.0	41.0
50-51	36.68914586174	39.0	35.0	40.0	30.0	41.0
52-53	36.57075639215104	39.0	35.0	40.0	30.0	41.0
54-55	36.41332487413924	38.0	35.0	40.0	29.5	41.0
56-57	36.2396518264105	38.0	34.5	40.0	29.0	41.0
58-59	36.06582960113499	38.0	34.0	40.0	29.0	41.0
60-61	35.86987109735245	38.0	34.0	40.0	28.0	41.0
62-63	35.652306884544004	37.5	34.0	40.0	28.0	41.0
64-65	35.38136704806231	37.0	34.0	40.0	28.0	41.0
66-67	35.114904423593835	36.5	33.0	39.0	28.0	41.0
68-69	34.82918242603438	36.0	33.0	39.0	28.0	41.0
70-71	34.526494245224896	35.5	33.0	39.0	27.5	40.0
72-73	34.22067177108134	35.0	32.0	38.5	27.0	40.0
74-75	33.902818850129506	35.0	32.0	37.5	26.0	40.0
76-77	32.08426483123886	33.5	30.0	35.5	25.0	38.5
78-79	33.21070087768085	34.5	31.5	36.0	26.0	39.0
80-81	33.284001688700116	35.0	32.0	36.0	27.0	39.0
82-83	33.07780061131129	35.0	32.0	36.0	26.5	37.5
84-85	32.85768359403305	34.0	32.0	35.5	26.0	37.0
86-87	32.65672346838642	34.0	31.0	35.0	26.0	37.0
88-89	32.500964677287946	34.0	31.0	35.0	26.0	36.0
90-91	32.37270509774855	34.0	31.0	35.0	26.0	36.0
92-93	32.303070260113344	34.0	31.0	35.0	26.0	36.0
94-95	32.31585828349038	34.0	31.0	35.0	26.5	35.0
96-97	32.40776170859738	34.0	31.0	35.0	27.0	35.0
98-99	32.56516143403305	34.0	31.0	35.0	27.5	35.0
100	30.97878908737284	33.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	1.0
14	0.0
15	0.0
16	0.0
17	3.0
18	6.0
19	41.0
20	312.0
21	1837.0
22	8928.0
23	29070.0
24	73466.0
25	147525.0
26	252841.0
27	379867.0
28	518512.0
29	658493.0
30	804358.0
31	959455.0
32	1143457.0
33	1383500.0
34	1720688.0
35	2189355.0
36	2946686.0
37	3981174.0
38	3939432.0
39	1851980.0
40	70954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.551649047814486	21.144292036079353	14.664170780044788	23.639888136061376
2	14.538437549101499	22.103295378946243	41.28853275092524	22.069734321027017
3	17.66696250758392	27.44424320863844	31.319640703316253	23.56915358046139
4	12.412894922782966	18.9678103906674	39.304859152166046	29.31443553438359
5	12.419299412950641	38.77400193262103	36.195021712175624	12.611676942252704
6	28.81137302165971	39.38278839881025	18.977037712994285	12.828800866535758
7	25.457959107428046	33.375081774346896	23.96622851183502	17.200730606390035
8	24.152650611212543	37.336625336674764	21.405341136664752	17.10538291544794
9	25.598656141101717	16.458581442285116	21.305063384723187	36.63769903188998
10-11	23.84638176122285	27.08090535657149	29.53548877614014	19.537224106065516
12-13	24.752953427925934	24.68453305949576	29.51007911508905	21.052434397489257
14-15	21.949033440611984	25.979063860247546	27.017454313634236	25.054448385506234
16-17	21.37089089748498	29.069409305286875	27.63870194618574	21.920997851042408
18-19	21.428089956313077	28.234386220915013	29.35350119703853	20.984022625733385
20-21	22.314361398945305	27.37702004792296	29.144402616023424	21.16421593710831
22-23	22.0045983436655	27.3211678194188	29.06325907189829	21.610974765017414
24-25	21.79290303772658	27.550461190303082	29.11860020329001	21.53803556868032
26-27	21.761282614209062	27.686809205744083	28.997372287568606	21.554535892478246
28-29	21.73052195698846	27.721110281325544	28.98720417654051	21.561163585145483
30-31	22.165652917938193	27.543908000641153	28.789093884503508	21.501345196917146
32-33	21.863029551798295	27.785510949261035	28.767051021325162	21.584408477615508
34-35	21.939989827656547	27.690036433204373	28.852707229103242	21.51726651003584
36-37	21.91182156970645	27.72521660550541	28.805304438099643	21.557657386688497
38-39	21.786215268235008	27.722290742690593	28.87925331251584	21.61224067655856
40-41	22.04101283112201	27.5652023220399	28.751909126450343	21.641875720387745
42-43	21.93349436820962	27.671692608282573	28.523306020050093	21.871507003457715
44-45	21.939696047048656	27.88125268877556	28.33446120369874	21.844590060477042
46-47	22.139404885096297	27.736970893447715	28.007253067551158	22.116371153904826
48-49	22.023640528731036	27.898704260589728	27.986873519246476	22.090781691432756
50-51	21.93560139381371	27.99579779534672	27.939409241177554	22.12919156966202
52-53	22.125426497927627	27.892109265191365	27.871657559220886	22.110806677660115
54-55	22.090047752239037	27.761351424977086	27.91116908805991	22.237431734723963
56-57	22.15192566453587	27.8924732566736	27.828710738891218	22.126890339899315
58-59	22.0406056764755	28.01653185118432	27.887456527547943	22.055405944792234
60-61	22.163799011419115	28.056813003532906	27.788819037058804	21.990568947989175
62-63	22.091265043058893	28.164930118791194	27.70577798913813	22.038026849011782
64-65	22.028603461295287	28.122795832086002	27.678702113652093	22.169898592966618
66-67	22.174707803305093	28.138039390896207	27.4699652216847	22.217287584113997
68-69	22.04759586738442	28.056434201033216	27.61943737260127	22.276532558981092
70-71	22.09450159326366	27.763041016294594	27.722631143761166	22.41982624668058
72-73	22.173249476928923	27.832194730890176	27.61830361446471	22.37625217771619
74-75	22.01360192408311	27.97084790458359	27.654990744673103	22.3605594266602
76-77	22.189465880235566	27.854327609859343	27.6261336860971	22.33007282380799
78-79	22.14827438749341	27.867703123746594	27.52983822246829	22.45418426629171
80-81	22.163206480098367	27.915399113173113	27.499037861726688	22.422356545001833
82-83	22.191794804427893	27.81784472869398	27.71152317623638	22.278837290641746
84-85	22.30603978399884	27.811152800732636	27.639634647503218	22.243172767765305
86-87	22.055450908106693	27.924671077428847	27.768845857469575	22.251032156994885
88-89	22.18728743970067	27.856777572124464	27.689028896135465	22.266906092039402
90-91	22.2902587951896	27.77200995031067	27.7938551011935	22.14387615330623
92-93	22.29558131360207	27.681415088991955	27.792486969807367	22.2305166275986
94-95	22.037668244012895	27.75742613719962	27.93562280273954	22.269282816047948
96-97	21.99406844606621	27.879153109797723	28.10355966869846	22.023218775437613
98-99	23.0617035052634	29.861288713429516	28.296448102499095	18.78055967880799
100	22.57213081755507	30.388763679019746	22.082952005921143	24.95615349750404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5023.0
1	4626.5
2	6951.0
3	10275.5
4	13619.0
5	21919.5
6	29601.0
7	35812.0
8	40457.0
9	40193.5
10	36001.5
11	31268.0
12	32382.0
13	41378.5
14	62954.0
15	97083.0
16	126122.0
17	141343.5
18	146440.5
19	142030.5
20	132844.0
21	123961.5
22	117967.0
23	122479.0
24	140875.5
25	173198.0
26	218897.0
27	270213.0
28	325168.0
29	389343.0
30	452212.5
31	511688.5
32	576358.5
33	635248.5
34	688903.5
35	736329.0
36	785203.5
37	830645.0
38	847695.0
39	846624.5
40	853851.0
41	862815.0
42	866601.0
43	876630.0
44	887811.5
45	902540.5
46	916872.0
47	925442.5
48	924040.5
49	909118.5
50	891012.0
51	872504.0
52	844103.0
53	803468.5
54	759761.5
55	712824.0
56	664090.0
57	607549.0
58	543261.0
59	477019.5
60	404157.5
61	330139.0
62	262194.5
63	204082.0
64	154357.5
65	114844.5
66	86483.5
67	66749.5
68	51928.5
69	37719.0
70	26467.0
71	18834.5
72	12977.0
73	8723.0
74	5959.0
75	4122.0
76	2830.5
77	1922.5
78	1337.0
79	973.5
80	713.0
81	525.5
82	402.5
83	285.5
84	203.0
85	147.5
86	114.0
87	90.5
88	60.0
89	37.0
90	25.5
91	19.5
92	15.5
93	7.0
94	1.5
95	0.5
96	0.0
97	0.5
98	1.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.12704045560771218
3	4.336147710004512E-6
4	0.0
5	0.0
6	0.0
7	2.601688626002707E-5
8	0.0
9	0.0
10-11	2.0813509008021656E-4
12-13	4.2927862329044666E-4
14-15	8.498849511608844E-4
16-17	2.8618574886029777E-4
18-19	0.0010385073765460805
20-21	6.287846739243017E-5
22-23	0.0019213792848899545
24-25	1.4751889489257044E-4
26-27	0.005845676400546355
28-29	0.002913107668068391
30-31	0.00500182450196927
32-33	0.01783253022597513
34-35	0.003974747483701401
36-37	0.002295844804585673
38-39	0.0012370488290392322
40-41	7.133328735308519E-4
42-43	0.001327945992009585
44-45	4.3113424612773374E-5
46-47	2.592176952183947E-4
48-49	1.7608334933143238E-4
50-51	5.754617091872439E-4
52-53	3.302846799149828E-5
54-55	0.0
56-57	0.0
58-59	1.3344486412449833E-4
60-61	0.0
62-63	2.7293995837020502E-5
64-65	2.5097265708115087E-5
66-67	2.5408628551741967E-6
68-69	3.012146441909739E-4
70-71	1.0437097012268782E-4
72-73	9.679673423687003E-4
74-75	9.648494103242425E-5
76-77	0.002246466067116474
78-79	0.0026949624325264955
80-81	0.002685029924393078
82-83	7.663933887613632E-5
84-85	0.0
86-87	0.0016477999920307468
88-89	4.001821724815028E-4
90-91	5.398730566285659E-4
92-93	6.246305895653898E-5
94-95	2.568322268198401E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	960.0
20-21	3237.0
22-23	6506.0
24-25	13346.0
26-27	15149.0
28-29	28015.0
30-31	56818.0
32-33	71314.0
34-35	99254.0
36-37	134875.0
38-39	160477.0
40-41	172997.0
42-43	199343.0
44-45	247178.0
46-47	219020.0
48-49	209798.0
50-51	187144.0
52-53	173420.0
54-55	188431.0
56-57	212031.0
58-59	222711.0
60-61	232903.0
62-63	226849.0
64-65	236556.0
66-67	257908.0
68-69	257888.0
70-71	260065.0
72-73	249488.0
74-75	254627.0
76-77	235611.0
78-79	263253.0
80-81	280553.0
82-83	276010.0
84-85	281216.0
86-87	302384.0
88-89	339160.0
90-91	375137.0
92-93	419079.0
94-95	502017.0
96-97	1005257.0
98-99	1649893.0
100-101	1.2534067E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.71097567096476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34529551263492	72.18780292277104
2	7.6639605861749835	12.524593940003538
3	2.037833321091106	4.9954004676347
4	0.85546234801677	2.796026524248987
5	0.4015466132066492	1.6405382771243402
6	0.21304143404036763	1.0444694060267954
7	0.12883250199774426	0.736892060545704
8	0.08955574606724574	0.5854149910476651
9	0.06287951721604215	0.46241520312978274
>10	0.19451466557038727	2.4413538411011078
>50	0.005404601376822998	0.29599781329878266
>100	0.001617735341618858	0.2299334308284408
>500	2.225680602066266E-5	0.010226247878927345
>1k	3.316045930419548E-5	0.04893487436008773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.336147710004512E-6	4.336147710004512E-6
2	0.0	4.336147710004512E-6	0.0	4.336147710004512E-6	4.336147710004512E-6
3	0.0	8.672295420009023E-6	0.0	8.672295420009023E-6	4.336147710004512E-6
4	0.0	3.468918168003609E-5	0.0	8.672295420009023E-6	4.336147710004512E-6
5	0.0	3.468918168003609E-5	0.0	8.672295420009023E-6	4.336147710004512E-6
6	0.0	3.468918168003609E-5	0.0	8.672295420009023E-6	4.336147710004512E-6
7	0.0	3.468918168003609E-5	0.0	8.672295420009023E-6	4.336147710004512E-6
8	0.0	3.9025329390040607E-5	0.0	8.672295420009023E-6	4.336147710004512E-6
9	0.0	8.672295420009024E-5	0.0	1.3008443130013535E-5	4.336147710004512E-6
10-11	0.0	8.672295420009024E-5	0.0	1.3008443130013535E-5	4.336147710004512E-6
12-13	0.0	8.88910280550925E-5	0.0	1.7344590840018047E-5	4.336147710004512E-6
14-15	0.0	1.3875672672014437E-4	0.0	2.601688626002707E-5	4.336147710004512E-6
16-17	0.0	1.517651698501579E-4	0.0	2.601688626002707E-5	4.336147710004512E-6
18-19	0.0	1.756139822551827E-4	0.0	3.2521107825033837E-5	4.336147710004512E-6
20-21	0.0	2.0379894237021205E-4	0.0	8.238680649008573E-5	4.336147710004512E-6
22-23	0.0	2.3415197634024364E-4	0.0	3.859171461904015E-4	4.336147710004512E-6
24-25	0.0	2.5800078874526846E-4	0.0	0.0013181889038413716	4.336147710004512E-6
26-27	0.0	2.7751345344028875E-4	0.0	0.00350794349739365	4.336147710004512E-6
28-29	0.0	2.8835382271530006E-4	0.0	0.013004106982303532	4.336147710004512E-6
30-31	0.0	3.208749305403339E-4	0.0	0.015488719620136118	4.336147710004512E-6
32-33	0.0	3.252110782503384E-4	0.0	0.02013706996526095	4.336147710004512E-6
34-35	0.0	3.794129246253948E-4	0.0	0.026073256180257128	4.336147710004512E-6
36-37	0.0	4.3361477100045117E-4	0.0	0.03576888245982722	4.336147710004512E-6
38-39	0.0	4.5963165726047825E-4	0.0	0.06769810612244544	4.336147710004512E-6
40-41	0.0	4.964889127955166E-4	0.0	0.10060513109366968	4.336147710004512E-6
42-43	0.0	5.268419467655482E-4	0.0	0.12074436913278563	4.336147710004512E-6
44-45	0.0	6.027245316906271E-4	0.0	0.14671789391571266	4.336147710004512E-6
46-47	0.0	6.590944519206858E-4	0.0	0.17353263135438057	6.5042215650067675E-6
48-49	0.0	6.612625257756881E-4	0.0	0.19831588359091135	8.672295420009023E-6
50-51	0.0	7.349770368457648E-4	0.0	0.22473603158796884	8.672295420009023E-6
52-53	0.0	7.414812584107715E-4	0.0	0.29328402266157516	8.672295420009023E-6
54-55	0.0	7.501535538307804E-4	0.0	0.3760979397010963	8.672295420009023E-6
56-57	0.0	7.63161996960794E-4	0.0	0.45380604281208714	8.672295420009023E-6
58-59	0.0	7.696662185258008E-4	0.0	0.49516638774396526	8.672295420009023E-6
60-61	0.0	7.783385139458099E-4	0.0	0.5188222415758948	8.672295420009023E-6
62-63	0.0	7.935150309308257E-4	0.0	0.5368736244926436	8.672295420009023E-6
64-65	0.0	8.759018374209114E-4	0.0	0.5442320671565213	8.672295420009023E-6
66-67	0.0	8.997506498259362E-4	0.0	0.5511091974245884	8.672295420009023E-6
68-69	0.0	9.084229452459452E-4	0.0	0.5577825287502853	8.672295420009023E-6
70-71	0.0	9.170952406659542E-4	0.0	0.5636601769711965	8.672295420009023E-6
72-73	0.0	9.257675360859632E-4	0.0	0.568044022306011	8.672295420009023E-6
74-75	0.0	9.452802007809836E-4	0.0	0.5716018315020698	8.672295420009023E-6
76-77	0.0	9.929778255910332E-4	0.0	0.5751401280334334	8.672295420009023E-6
78-79	0.0	0.0010189947118510604	0.0	0.5773905886949258	8.672295420009023E-6
80-81	4.336147710004512E-6	0.0010189947118510604	0.0	0.5781364061010466	8.672295420009023E-6
82-83	4.336147710004512E-6	0.0010276670072710695	0.0	0.5787824921098372	8.672295420009023E-6
84-85	4.336147710004512E-6	0.0010450115981110873	0.0	0.5790686778586975	8.672295420009023E-6
86-87	4.336147710004512E-6	0.0010536838935310963	0.0	0.5791597369606076	8.672295420009023E-6
88	4.336147710004512E-6	0.0010883730752111324	0.0	0.5791705773298826	8.672295420009023E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	29660	0.0	50.573486	1
GTATCAA	53975	0.0	36.313446	1
TCAACGC	68475	0.0	28.04892	4
ATCAACG	69010	0.0	27.837479	3
CAACGCA	69880	0.0	27.479156	5
AACGCAG	71615	0.0	26.923437	6
TATCAAC	71850	0.0	26.921053	2
ACGCAGA	82620	0.0	23.271986	7
CGCAGAG	84030	0.0	22.886421	8
GTACATG	75195	0.0	20.986258	1
TACATGG	74620	0.0	20.58603	2
ACATGGG	75705	0.0	19.958754	3
GCAGAGT	97000	0.0	19.642775	9
GTGGTAT	13355	0.0	19.211914	1
AGAGTAC	88240	0.0	18.432735	10-11
GAGTACT	56170	0.0	18.39701	12-13
CAGAGTA	95275	0.0	17.770218	10-11
CATGGGG	56395	0.0	17.543226	4
TGGTATC	13430	0.0	17.466204	2
AGTACTT	61110	0.0	16.668947	12-13
>>END_MODULE