FastQCFastQC Report
Fri 27 May 2016
SRR2139339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21400075
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA270400.049.403821
GTATCAA480350.034.9897041
ATCAACG599450.027.3457813
TCAACGC599450.027.3457814
CAACGCA611650.026.8209065
AACGCAG626650.026.2255866
TATCAAC630450.026.161352
ACGCAGA717850.022.8357727
AGACCGA179400.022.80022688-89
GACCGAG180950.022.73275690-91
ACCGAGG186050.022.64845590-91
CGCAGAG729550.022.4695478
GAGACCG179450.022.45718488-89
CGAGACC183350.022.33763986-87
GTACATG623300.021.459221
TACATGG626300.020.8619462
ACGAGAC194600.020.74410886-87
CACGAGA199500.020.40018384-85
ACATGGG635600.020.2667683
CCCACGA202350.019.8433682-83
Produced by FastQC (version 0.11.5)