##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21400075
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.595500763431904	31.0	31.0	34.0	30.0	34.0
2	31.683837136084804	31.0	31.0	34.0	30.0	34.0
3	31.767733571027204	31.0	31.0	34.0	30.0	34.0
4	35.54200169859218	37.0	35.0	37.0	33.0	37.0
5	35.393426985653086	37.0	35.0	37.0	33.0	37.0
6	35.42401458873392	37.0	35.0	37.0	33.0	37.0
7	35.327234367169275	37.0	35.0	37.0	32.0	37.0
8	35.29907483969098	37.0	35.0	37.0	32.0	37.0
9	36.902447818524	39.0	37.0	39.0	33.0	39.0
10-11	36.79151136152561	39.0	36.0	39.0	32.0	39.0
12-13	36.748471161900135	39.0	35.0	39.0	32.0	39.0
14-15	37.86128497213211	40.0	37.0	41.0	32.0	41.0
16-17	37.81860570582113	40.0	37.0	41.0	32.0	41.0
18-19	37.83837383747487	40.0	37.0	41.0	32.0	41.0
20-21	37.800041856077556	40.0	37.0	41.0	32.0	41.0
22-23	37.713209609160806	40.0	37.0	41.0	32.0	41.0
24-25	37.60762502292303	39.0	36.0	41.0	32.0	41.0
26-27	37.38546741488022	39.0	36.0	41.0	31.5	41.0
28-29	37.23029476187523	39.0	36.0	41.0	31.0	41.0
30-31	37.05042533863222	39.0	36.0	40.5	31.0	41.0
32-33	36.85700648399082	39.0	35.5	40.0	30.0	41.0
34-35	36.70950840569419	38.5	35.0	40.0	30.0	41.0
36-37	36.53180306488407	38.0	35.0	40.0	30.0	41.0
38-39	36.37351411947603	38.0	35.0	40.0	30.0	41.0
40-41	36.22115281052545	38.0	35.0	40.0	30.0	41.0
42-43	36.01497149218841	38.0	34.5	40.0	29.0	41.0
44-45	36.1004802608838	38.0	34.5	40.0	29.5	41.0
46-47	36.57411073160789	38.5	35.0	40.0	30.0	41.0
48-49	36.67288595098632	39.0	35.0	40.0	30.0	41.0
50-51	36.63122087866087	39.0	35.0	40.0	30.0	41.0
52-53	36.51084219364007	38.5	35.0	40.0	30.0	41.0
54-55	36.35018924987784	38.0	35.0	40.0	29.5	41.0
56-57	36.17759883812376	38.0	34.0	40.0	29.0	41.0
58-59	36.00350022592621	38.0	34.0	40.0	28.5	41.0
60-61	35.80973566209174	38.0	34.0	40.0	28.0	41.0
62-63	35.582990839737896	37.0	34.0	40.0	28.0	41.0
64-65	35.31605771452536	37.0	33.5	40.0	28.0	41.0
66-67	35.046399329707874	36.5	33.0	39.0	28.0	41.0
68-69	34.75826831842113	36.0	33.0	39.0	28.0	41.0
70-71	34.4502967389238	35.5	32.5	39.0	27.0	40.0
72-73	34.13758069427382	35.0	32.0	38.0	26.5	40.0
74-75	33.81924395096161	35.0	32.0	37.5	26.0	39.5
76-77	32.00128021694891	33.5	29.5	35.5	25.0	38.5
78-79	33.128255889453825	34.5	31.5	36.0	26.0	39.0
80-81	33.189363844753956	35.0	32.0	36.0	26.0	39.0
82-83	32.98639139905984	34.5	32.0	36.0	26.0	37.5
84-85	32.77399418660334	34.0	31.0	35.0	26.0	37.0
86-87	32.57538405364396	34.0	31.0	35.0	26.0	36.5
88-89	32.41630984508805	34.0	31.0	35.0	26.0	36.0
90-91	32.29189789958326	34.0	31.0	35.0	26.0	36.0
92-93	32.22388951391433	34.0	31.0	35.0	26.0	36.0
94-95	32.23566976116564	34.0	31.0	35.0	26.0	35.0
96-97	32.33978318783258	34.0	31.0	35.0	27.0	35.0
98-99	32.51201696422609	34.0	31.0	35.0	27.0	35.0
100	30.91888720044182	33.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	1.0
18	9.0
19	40.0
20	260.0
21	1757.0
22	8216.0
23	27472.0
24	69125.0
25	140474.0
26	241533.0
27	362660.0
28	495470.0
29	631055.0
30	769143.0
31	917178.0
32	1089980.0
33	1314614.0
34	1631003.0
35	2064869.0
36	2766919.0
37	3703891.0
38	3586016.0
39	1528837.0
40	49550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.64858651196316	20.996276882207187	14.577023678655332	23.778112927174323
2	14.728003075192115	22.12015613262402	40.820447669520156	22.33139312266371
3	17.91494823405509	27.057818914916787	31.116206940041746	23.91102591098638
4	12.580596096041718	18.812396685525634	39.04492390797696	29.56208331045569
5	12.64815660692778	38.54576210597393	36.0624483792697	12.743632907828594
6	29.3051169213192	39.10935826159488	18.77972857571761	12.805796241368315
7	25.695581771874416	33.14703800996558	23.828401138089628	17.328979080070383
8	24.259732734581537	37.171804304424164	21.281191771524167	17.28727118947013
9	25.614438267155602	16.328405391102603	21.201701395906323	36.85545494583547
10-11	23.90319734327506	26.936795539913316	29.479055697695788	19.680951419115836
12-13	24.78555930888324	24.605589455650247	29.412254398638726	21.196596836827787
14-15	21.97507179998337	25.937660317626243	26.92014385767822	25.167124024712173
16-17	21.465282686263663	28.963217033409418	27.599386149934297	21.972114130392626
18-19	21.534852313427137	28.153212285676492	29.267277802963555	21.044657597932808
20-21	22.36602452857818	27.34658387897091	29.08178928887863	21.205602303572284
22-23	22.05543101096129	27.267416037565535	28.99040821608711	21.68674473538607
24-25	21.86861649347475	27.43423057425157	28.988959051930458	21.708193880343227
26-27	21.844436118420944	27.539616312228592	28.910949342353696	21.704998226996768
28-29	21.807679392384703	27.582934439098544	28.90924640679171	21.700139761725044
30-31	22.127640510632933	27.46230691979916	28.76011241310122	21.64994015646668
32-33	21.91813177292673	27.640763914541168	28.724211251335397	21.716893061196707
34-35	22.011913670205995	27.66263936674766	28.747860260400483	21.577586702645863
36-37	22.02504952936475	27.62233966849556	28.65662094990698	21.695989852232707
38-39	21.88812503873116	27.716781982777622	28.697039806448192	21.69805317204302
40-41	22.13820234729506	27.55984712918154	28.5752869206444	21.726663602879
42-43	21.98461928920883	27.6957494309834	28.32781204819848	21.991819231609288
44-45	22.057145249365764	27.822948585299002	28.15412717194297	21.965778993392263
46-47	22.174816474603052	27.80178810938982	27.901394576823996	22.12200083918313
48-49	22.08374401899797	27.92132840670617	27.874518265746485	22.12040930854937
50-51	22.10990926768611	28.005782478529724	27.73869280376954	22.14561545001463
52-53	22.213330458234992	27.875699498952038	27.678700270668983	22.232269772143987
54-55	22.18415856633735	27.766941067075674	27.787591232312703	22.261309134274278
56-57	22.198676453499225	27.8521752550567	27.738341930989087	22.210806360454992
58-59	22.113620082389332	27.835887311370865	27.687175749113656	22.363316857126147
60-61	22.359338193488952	27.81836709626644	27.814688487743645	22.007606222500964
62-63	22.10301879270165	27.876798827266352	27.765441752937974	22.254740627094023
64-65	22.04500755302897	27.85815970051415	27.71844441452253	22.37838833193435
66-67	22.130963513063016	27.916957658418756	27.74397437839896	22.208104450119272
68-69	22.062458061270142	28.0621516810524	27.661586599193793	22.213803658483666
70-71	22.09093956113655	27.79795152927435	27.537974225367424	22.573134684221678
72-73	22.14577596570729	27.81984410020038	27.46053931370409	22.573840620388236
74-75	22.02959121915211	27.9975361516823	27.650758330030477	22.322114299135112
76-77	22.18155618543908	27.662851500620185	27.649509305469284	22.506083008471446
78-79	22.110959493224488	27.686343061344477	27.417765486899032	22.784931958532002
80-81	22.329360950998684	27.91917983726224	27.36575836997046	22.385700841768607
82-83	22.36994396291508	27.653706583224658	27.505850560036478	22.470498893823788
84-85	22.22998670872156	27.642187006774314	27.46983672076565	22.65798956373847
86-87	22.055967483117662	27.887903245297775	27.574226106933448	22.481903164651115
88-89	22.338930614044365	27.841828212568448	27.561549313117716	22.257691860269468
90-91	22.427154423583353	27.731832552717687	27.66298440816618	22.178028615532778
92-93	22.231209689926217	27.47308667480422	27.661980414240876	22.63372322102869
94-95	22.20853417866625	27.74314589242377	27.801846309439625	22.246473619470354
96-97	22.410276492722318	27.667170905085143	27.929183888610005	21.993368713582537
98-99	23.117593325050947	29.761615856915917	28.304738204183067	18.81605261385007
100	22.57888548940683	30.302641431441707	22.05646076647591	25.062012312675552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4901.0
1	4358.0
2	6283.0
3	9214.5
4	12126.0
5	19619.0
6	26385.5
7	31656.5
8	35440.0
9	35070.0
10	31604.0
11	27683.0
12	29133.0
13	37753.0
14	57695.5
15	88810.0
16	115158.0
17	128828.5
18	133297.0
19	129532.0
20	121495.5
21	113030.5
22	106410.0
23	109022.0
24	124279.0
25	150844.0
26	188047.5
27	229850.0
28	275286.5
29	328270.5
30	381534.5
31	435053.5
32	491672.0
33	542135.5
34	588455.5
35	632854.5
36	678062.5
37	721306.0
38	751073.5
39	770765.5
40	794386.0
41	816665.5
42	830693.0
43	846524.0
44	862240.5
45	876505.5
46	883838.0
47	884481.0
48	881813.5
49	871247.0
50	857693.5
51	834888.5
52	798022.5
53	755621.0
54	712676.0
55	664171.5
56	611830.5
57	557053.0
58	496522.5
59	432650.5
60	364479.0
61	297801.0
62	237672.0
63	184747.5
64	139664.5
65	104565.0
66	79032.5
67	60855.0
68	47072.0
69	34387.0
70	24885.0
71	18321.5
72	13098.0
73	9079.0
74	6320.0
75	4475.5
76	3129.0
77	2150.5
78	1532.0
79	1108.5
80	775.0
81	535.0
82	386.5
83	265.0
84	184.5
85	144.5
86	115.0
87	90.5
88	73.0
89	56.5
90	39.5
91	30.0
92	24.0
93	16.0
94	10.0
95	8.0
96	7.0
97	6.0
98	2.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.12360237055243964
3	9.345761638685845E-6
4	0.0
5	0.0
6	0.0
7	1.401864245802877E-5
8	0.0
9	0.0
10-11	1.9859743482207422E-4
12-13	4.766338435729781E-4
14-15	8.154177029753401E-4
16-17	2.850457299799183E-4
18-19	9.883142932910283E-4
20-21	6.07516280209594E-5
22-23	0.0018088338819545195
24-25	1.4026614331810082E-4
26-27	0.005450059224366752
28-29	0.0027832185916942015
30-31	0.004594661968609175
32-33	0.017263243405762973
34-35	0.003920879804875519
36-37	0.002176599063726084
38-39	0.0012436702366911737
40-41	6.78864722290955E-4
42-43	0.001293024295854013
44-45	1.9516966306177728E-5
46-47	2.366529288819739E-4
48-49	1.7169329048996837E-4
50-51	4.995777814614318E-4
52-53	2.5303018192085684E-5
54-55	0.0
56-57	0.0
58-59	1.6333071459754262E-4
60-61	0.0
62-63	1.5850034162106963E-5
64-65	4.2711674520809716E-5
66-67	0.0
68-69	2.3500389587272495E-4
70-71	9.685457570487092E-5
72-73	9.277394920959818E-4
74-75	1.476240072179055E-4
76-77	0.002059240139813202
78-79	0.002633329722214883
80-81	0.0026058256215765667
82-83	7.197270003514967E-5
84-85	0.0
86-87	0.0016463283736246929
88-89	3.781023772682834E-4
90-91	5.950677886750236E-4
92-93	6.913150550510639E-5
94-95	4.0595016034862186E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	911.0
20-21	2967.0
22-23	5664.0
24-25	11306.0
26-27	13645.0
28-29	24945.0
30-31	49731.0
32-33	64067.0
34-35	88656.0
36-37	118771.0
38-39	138954.0
40-41	151437.0
42-43	176163.0
44-45	220692.0
46-47	192537.0
48-49	180367.0
50-51	161219.0
52-53	152766.0
54-55	164837.0
56-57	182407.0
58-59	123413.0
60-61	198906.0
62-63	195391.0
64-65	205394.0
66-67	221229.0
68-69	227325.0
70-71	230777.0
72-73	226119.0
74-75	232970.0
76-77	213570.0
78-79	237318.0
80-81	250187.0
82-83	250884.0
84-85	258673.0
86-87	280859.0
88-89	313852.0
90-91	348776.0
92-93	392161.0
94-95	474406.0
96-97	1028467.0
98-99	1581636.0
100-101	1.180572E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.59333436587501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.64418121640595	73.21418498795835
2	7.55352861228695	12.477422286336399
3	1.993318065024877	4.93904356326416
4	0.7848110130775767	2.592806336685496
5	0.38798115008668976	1.6022328428383352
6	0.2104783338084206	1.0430464440606682
7	0.126101478715745	0.729059911692056
8	0.0745035777419514	0.49227991263159643
9	0.05404443691229169	0.40173392236611105
>10	0.166294301471123	2.1075322507309857
>50	0.0036220729410212068	0.19606351112109588
>100	0.001050393163186578	0.13908378445021996
>500	5.343110210574687E-5	0.03064303990706382
>1k	3.1917262118759084E-5	0.03486720595746453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4018642458028768E-5	9.345761638685845E-6
2	0.0	4.672880819342923E-5	0.0	1.4018642458028768E-5	9.345761638685845E-6
3	0.0	4.672880819342923E-5	0.0	2.8037284916057536E-5	9.345761638685845E-6
4	0.0	1.0747625884488723E-4	0.0	3.738304655474338E-5	9.345761638685845E-6
5	4.672880819342923E-6	1.1214913966423015E-4	0.0	4.672880819342923E-5	9.345761638685845E-6
6	1.869152327737169E-5	1.1214913966423015E-4	0.0	4.672880819342923E-5	9.345761638685845E-6
7	1.869152327737169E-5	1.1214913966423015E-4	0.0	5.140168901277215E-5	9.345761638685845E-6
8	1.869152327737169E-5	1.3084066294160183E-4	0.0	7.94389739288297E-5	9.345761638685845E-6
9	1.869152327737169E-5	2.523355642445178E-4	0.0	8.411185474817261E-5	9.345761638685845E-6
10-11	1.869152327737169E-5	2.523355642445178E-4	0.0	1.1682202048357308E-4	9.345761638685845E-6
12-13	1.869152327737169E-5	2.523355642445178E-4	0.0	1.401864245802877E-4	9.345761638685845E-6
14-15	1.869152327737169E-5	3.3878385940236194E-4	0.0	1.5186862662864499E-4	2.3364404096714615E-5
16-17	2.8037284916057536E-5	3.5280250186039063E-4	0.0	2.0093387523174568E-4	3.738304655474338E-5
18-19	2.8037284916057536E-5	3.7149402513776236E-4	0.0	2.3364404096714615E-4	3.738304655474338E-5
20-21	2.8037284916057536E-5	3.995313100538199E-4	0.0	2.967279320282756E-4	3.971948696441484E-5
22-23	2.8037284916057536E-5	4.7663384357297814E-4	0.0	5.887829832372083E-4	6.308389106112947E-5
24-25	2.8037284916057536E-5	5.490634962727934E-4	0.0	0.0014415837327672917	7.94389739288297E-5
26-27	2.8037284916057536E-5	5.747643407791795E-4	0.0	0.003483632650820149	7.94389739288297E-5
28-29	2.8037284916057536E-5	5.747643407791795E-4	0.0	0.012151826570701272	8.411185474817261E-5
30-31	2.8037284916057536E-5	6.401846722499804E-4	0.0	0.014289669545550658	9.345761638685846E-5
32-33	2.8037284916057536E-5	6.448575530693234E-4	0.0	0.01859572922057516	1.1682202048357308E-4
34-35	2.8037284916057536E-5	7.383151694561817E-4	0.0	0.02399524300732591	1.3084066294160183E-4
36-37	2.8037284916057536E-5	8.224270242043544E-4	0.0	0.03330362159945701	1.3084066294160183E-4
38-39	2.8037284916057536E-5	8.50464309120412E-4	0.0	0.06259557501550812	1.331771033512733E-4
40-41	2.8037284916057536E-5	9.906507337006996E-4	0.0	0.09249733937848348	1.401864245802877E-4
42-43	2.8037284916057536E-5	0.0010210244590264286	0.0	0.11149259990911248	1.401864245802877E-4
44-45	2.8037284916057536E-5	0.0011565380027873736	0.0	0.13672381989315457	1.401864245802877E-4
46-47	2.8037284916057536E-5	0.0012616778212225892	0.0	0.16212092714628334	1.5186862662864499E-4
48-49	2.8037284916057536E-5	0.001271023582861275	0.0	0.1862773845418766	1.588779478576594E-4
50-51	3.0373725325729E-5	0.0013738269608868192	0.0	0.21144084775403824	1.588779478576594E-4
52-53	3.271016573540046E-5	0.001387845603344848	0.0	0.2737396948375181	1.8925167318338838E-4
54-55	3.271016573540046E-5	0.0013971913649835337	0.0	0.35187493501775113	2.2663471973813175E-4
56-57	3.271016573540046E-5	0.0014158828882609056	0.0	0.42836298470916573	2.3364404096714615E-4
58-59	3.271016573540046E-5	0.0014252286498995913	0.0	0.6371075802304431	2.3831692178648907E-4
60-61	3.271016573540046E-5	0.0014462566135866346	0.0	0.8170228375367843	2.546720046541893E-4
62-63	3.271016573540046E-5	0.0014719574580930208	0.0	0.8258732738086199	2.803728491605754E-4
64-65	3.271016573540046E-5	0.0016799006545537808	0.0	0.8328755857164052	2.850457299799183E-4
66-67	3.971948696441484E-5	0.001717283701108524	0.0	0.839476029873727	2.9205505120893265E-4
68-69	4.672880819342923E-5	0.0017640125093019534	0.0	0.8455671300217406	2.9439149161860414E-4
70-71	4.672880819342923E-5	0.0017850404729889966	0.0	0.8510133726166847	3.084101340766329E-4
72-73	5.140168901277215E-5	0.0017897133538083395	0.0	0.8551909280691774	3.5981182308940504E-4
74-75	5.140168901277215E-5	0.00182242351954374	0.0	0.8584455895598497	3.5981182308940504E-4
76-77	5.140168901277215E-5	0.0018925167318338838	0.0	0.8617609985011735	3.5981182308940504E-4
78-79	5.140168901277215E-5	0.001929899778388627	0.0	0.8640507101026516	3.5981182308940504E-4
80-81	5.140168901277215E-5	0.001929899778388627	0.0	0.8647493057851433	3.761669059571053E-4
82-83	5.140168901277215E-5	0.001929899778388627	0.0	0.865291359960187	4.1588639292152016E-4
84-85	5.140168901277215E-5	0.0019485913016659988	0.0	0.8655927607730347	4.1588639292152016E-4
86-87	5.140168901277215E-5	0.002007002311907785	0.0	0.8657282743167956	4.1588639292152016E-4
88	5.140168901277215E-5	0.002074759083788258	0.0	0.8657352836380247	4.1588639292152016E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	27040	0.0	49.40382	1
GTATCAA	48035	0.0	34.989704	1
ATCAACG	59945	0.0	27.345781	3
TCAACGC	59945	0.0	27.345781	4
CAACGCA	61165	0.0	26.820906	5
AACGCAG	62665	0.0	26.225586	6
TATCAAC	63045	0.0	26.16135	2
ACGCAGA	71785	0.0	22.835772	7
AGACCGA	17940	0.0	22.800226	88-89
GACCGAG	18095	0.0	22.732756	90-91
ACCGAGG	18605	0.0	22.648455	90-91
CGCAGAG	72955	0.0	22.469547	8
GAGACCG	17945	0.0	22.457184	88-89
CGAGACC	18335	0.0	22.337639	86-87
GTACATG	62330	0.0	21.45922	1
TACATGG	62630	0.0	20.861946	2
ACGAGAC	19460	0.0	20.744108	86-87
CACGAGA	19950	0.0	20.400183	84-85
ACATGGG	63560	0.0	20.266768	3
CCCACGA	20235	0.0	19.84336	82-83
>>END_MODULE