FastQCFastQC Report
Fri 27 May 2016
SRR2139338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18073499
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214600.052.567221
ATCTCGT138850.045.9403794
TATCTCG168300.036.10307394
GTATCAA387700.035.7639851
CCGTCTT74200.031.49192690-91
GCCGTCT79650.031.25892890-91
TATGCCG72950.031.06922386-87
ATGCCGT79000.030.45472588-89
TGCCGTC80200.028.83658288-89
TCAACGC491400.027.6737524
TCGTATG89000.027.57339984-85
ATCAACG495800.027.4111463
CAACGCA504050.026.9624945
TCTCGTA87200.026.69535882-83
CTCGTAT84700.026.49350282-83
TATCAAC520600.026.3176732
AACGCAG519500.026.2661786
CGTATGC90700.025.73521884-85
CGCTATC257500.025.30106294
CTATCTC269150.023.55930194
Produced by FastQC (version 0.11.5)