##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18073499
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.362332827749626	31.0	31.0	34.0	30.0	34.0
2	31.52479970812514	31.0	31.0	34.0	30.0	34.0
3	31.564959834285546	31.0	31.0	34.0	30.0	34.0
4	35.38350476573463	37.0	35.0	37.0	33.0	37.0
5	35.15464974435775	37.0	35.0	37.0	32.0	37.0
6	35.18081927578052	37.0	35.0	37.0	32.0	37.0
7	35.05957900017036	37.0	35.0	37.0	32.0	37.0
8	35.033325423040665	37.0	35.0	37.0	32.0	37.0
9	36.56419772397143	39.0	35.0	39.0	32.0	39.0
10-11	36.46427191547137	38.0	35.0	39.0	32.0	39.0
12-13	36.3900708988337	38.0	35.0	39.0	32.0	39.0
14-15	37.44550097908545	39.0	36.0	41.0	32.0	41.0
16-17	37.430162001281545	39.0	36.0	41.0	32.0	41.0
18-19	37.4474773534444	39.0	36.0	41.0	32.0	41.0
20-21	37.42717921710473	39.0	36.0	41.0	32.0	41.0
22-23	37.33491719075539	39.0	36.0	41.0	31.0	41.0
24-25	37.22805296166317	39.0	36.0	41.0	31.0	41.0
26-27	37.00197028582749	39.0	36.0	40.5	31.0	41.0
28-29	36.82866073718908	39.0	36.0	40.0	30.0	41.0
30-31	36.66720518805276	38.5	35.0	40.0	30.0	41.0
32-33	36.46421637050172	38.0	35.0	40.0	30.0	41.0
34-35	36.30376282560192	38.0	35.0	40.0	30.0	41.0
36-37	36.15607582347117	38.0	35.0	40.0	30.0	41.0
38-39	35.994258828857824	38.0	34.0	40.0	29.0	41.0
40-41	35.84573953972184	38.0	34.0	40.0	29.0	41.0
42-43	35.68204099382746	38.0	34.0	40.0	28.0	41.0
44-45	35.51595993566052	38.0	33.5	40.0	27.5	41.0
46-47	35.820957299140105	38.0	34.0	40.0	28.5	41.0
48-49	36.19402346961373	38.0	34.5	40.0	29.0	41.0
50-51	36.20397222047782	38.0	34.5	40.0	29.0	41.0
52-53	36.10853503085983	38.0	34.0	40.0	29.0	41.0
54-55	35.99314224393892	38.0	34.0	40.0	28.0	41.0
56-57	35.84298769034265	38.0	34.0	40.0	28.0	41.0
58-59	35.670387981937665	38.0	34.0	40.0	28.0	41.0
60-61	35.476333487478314	37.5	33.5	40.0	28.0	41.0
62-63	35.26575617310375	37.0	33.0	40.0	28.0	41.0
64-65	34.97881157064247	36.5	33.0	39.5	27.5	41.0
66-67	34.708686320803906	36.0	33.0	39.0	27.0	41.0
68-69	34.43981641852338	36.0	32.0	39.0	26.5	40.5
70-71	34.171221227454254	35.0	32.0	39.0	26.0	40.0
72-73	33.88219694679584	35.0	32.0	38.0	26.0	40.0
74-75	33.550733103287484	35.0	31.5	37.0	26.0	39.5
76-77	31.80817207547614	33.5	29.5	35.5	25.0	38.5
78-79	32.85517169191479	34.0	31.0	36.0	26.0	39.0
80-81	32.91272978217505	34.0	31.0	36.0	26.0	38.5
82-83	32.69681305929477	34.0	31.0	35.5	26.0	37.0
84-85	32.50013891129367	34.0	31.0	35.0	26.0	37.0
86-87	32.299418848921604	34.0	31.0	35.0	26.0	36.5
88-89	32.14034959114803	34.0	31.0	35.0	26.0	36.0
90-91	32.0649307389821	34.0	31.0	35.0	26.0	36.0
92-93	32.034174064961775	34.0	31.0	35.0	26.0	35.5
94-95	32.022579712259095	34.0	31.0	35.0	26.0	35.0
96-97	32.12095428136444	34.0	31.0	35.0	26.0	35.0
98-99	32.31435102331828	34.0	31.0	35.0	27.0	35.0
100	30.83290821724699	32.0	29.0	34.0	24.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	43.0
20	273.0
21	1902.0
22	9028.0
23	30378.0
24	74366.0
25	148535.0
26	249789.0
27	369653.0
28	496256.0
29	622250.0
30	743251.0
31	872298.0
32	1018582.0
33	1204003.0
34	1455968.0
35	1796842.0
36	2314706.0
37	2932524.0
38	2682717.0
39	1017894.0
40	32238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.52749276717253	21.033099346175305	14.627117859137293	23.81229002751487
2	14.791753161716075	22.02652873109311	40.71972523687594	22.461992870314877
3	17.788022120121845	27.093331512619667	31.140959478848007	23.977686888410485
4	12.546804729622284	18.692367950546807	39.0724173200821	29.688409999748806
5	12.697557899552267	38.52655758577794	36.016617479548366	12.759267035121422
6	29.442948484961324	39.03533565913275	18.719535160291873	12.80218069561406
7	25.720382090927718	33.17670806300429	23.828224960756078	17.274684885311913
8	24.34846180034093	37.09623133849178	21.229895771704197	17.325411089463085
9	25.692363166645265	16.33717411332471	21.179595605698708	36.79086711433132
10-11	23.96064812906455	26.88641529789002	29.46895617721837	19.68398039582706
12-13	24.793533209662648	24.59461174028979	29.361387107349007	21.250467942698553
14-15	22.01485473441923	25.9195570276422	26.844780543742207	25.220807694196363
16-17	21.51866085640376	28.959501759304562	27.529870552753756	21.991966831537926
18-19	21.583081394477073	28.16386024643042	29.14744400074385	21.10561435834865
20-21	22.466726976531888	27.380569064469825	28.945185658676305	21.207518300321993
22-23	22.145959772458625	27.23508547661438	28.852116229943185	21.766838520983818
24-25	21.918805149044392	27.4264555300617	28.87966722737405	21.775072093519857
26-27	21.907112082922218	27.54560433390675	28.75406773247171	21.793215850699323
28-29	21.89699900968157	27.553496518249755	28.810168526015723	21.739335946052957
30-31	22.210348530905545	27.45332049449587	28.60033443757552	21.73599653702306
32-33	21.9841188353345	27.639567917561358	28.569694594978312	21.80661865212583
34-35	22.10515148864001	27.623212755293196	28.562609564157736	21.709026191909057
36-37	22.09991939007551	27.544654689031283	28.523202956355515	21.832222964537692
38-39	21.912937909697465	27.67506275609285	28.551837004834308	21.860162329375378
40-41	22.249287926068696	27.455975459790196	28.536684882205982	21.758051731935126
42-43	21.979838077743594	27.611558921925695	28.282386713993375	22.12621628633734
44-45	22.08660233757616	27.66347296280678	28.102051076165512	22.14787362345155
46-47	22.16986490333595	27.63865582113059	27.987742439933694	22.203736835599763
48-49	22.010659435471037	27.905602120962776	27.9201620088534	22.16357643471279
50-51	22.015644208419598	27.9949455742039	27.676701207632092	22.31270900974441
52-53	22.080574231151164	27.76036384699318	27.616050992563334	22.543010929292322
54-55	22.192140505107076	27.497163396073216	27.925250592750555	22.38544550606915
56-57	22.00659947294171	27.312277183717164	28.0588549917134	22.62226835162772
58-59	21.878200487703914	27.131498051971754	28.075277312103346	22.915024148220983
60-61	22.050927394159004	27.334149084972548	28.33996433461694	22.274959186251508
62-63	21.653627676450206	27.564681328104374	28.290219364384207	22.49147163106122
64-65	21.551699093755396	27.856497464862645	27.927380032395526	22.66442340898643
66-67	21.59322047329312	28.41208786769075	27.495418650586963	22.49927300842917
68-69	21.664378758993138	28.42693222695008	27.269907339581284	22.638781674475496
70-71	21.67689283942171	27.86290058288481	27.285894935308136	23.174311642385348
72-73	21.903050690510717	27.467082422356988	27.249618880167027	23.380248006965267
74-75	21.897350332462697	27.512072484634327	27.485079497190945	23.105497685712034
76-77	22.27401203861998	27.372541591522868	26.980943820850552	23.3725025490066
78-79	22.45283173598603	27.469465541815048	26.474346256181295	23.603356466017626
80-81	22.33298459799834	27.762040358552436	26.622240867101016	23.28273417634821
82-83	22.266964281335667	27.52081014703851	26.751736130977072	23.46048944064875
84-85	22.303250690161406	27.87066281111677	26.764086682668285	23.06199981605354
86-87	22.703731547165916	27.77717138006554	26.884999065133535	22.634098007635007
88-89	22.476865168579625	27.894612670960655	27.165415023712203	22.463107136747517
90-91	22.441236138440185	27.44087872950245	27.22662403480894	22.89126109724843
92-93	22.43306480838564	26.97835694257553	27.422265051138435	23.166313197900397
94-95	21.678439273662796	27.40955733222045	28.078739379397984	22.833264014718765
96-97	21.7723782048012	27.3660399587215	28.233292143811834	22.62828969266547
98-99	22.91000684620725	29.279576978145045	28.649794625234858	19.160621550412845
100	22.409149958456403	30.035658212018728	22.20230913609242	25.352882693432445
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3897.0
1	3504.5
2	4972.0
3	7223.0
4	9469.0
5	15449.5
6	20983.5
7	25054.5
8	27925.0
9	27727.0
10	25246.0
11	22311.5
12	23474.5
13	29986.5
14	46833.5
15	71335.5
16	90923.5
17	102408.5
18	106768.5
19	104722.0
20	99163.5
21	91906.0
22	86714.5
23	89532.0
24	101801.5
25	122593.0
26	152278.5
27	186179.5
28	221556.5
29	264292.5
30	306196.0
31	347297.0
32	394295.5
33	438311.5
34	478113.0
35	515724.5
36	557770.0
37	596502.0
38	620899.5
39	637201.5
40	658732.5
41	678666.0
42	690935.0
43	706143.0
44	719510.5
45	731894.0
46	736332.5
47	734785.5
48	735879.5
49	726651.0
50	712781.5
51	695881.5
52	669395.5
53	638011.0
54	604251.5
55	566965.5
56	525668.0
57	478925.5
58	427985.0
59	375755.0
60	317315.5
61	258408.0
62	205552.5
63	159707.0
64	120463.0
65	89894.5
66	67872.0
67	51778.5
68	39900.0
69	29472.0
70	21454.5
71	15895.0
72	11451.0
73	7939.5
74	5475.5
75	3871.0
76	2739.0
77	1919.5
78	1342.0
79	950.5
80	677.5
81	478.5
82	367.0
83	261.5
84	178.5
85	137.0
86	109.0
87	85.5
88	60.5
89	39.0
90	26.5
91	18.5
92	13.5
93	8.5
94	4.0
95	3.5
96	4.0
97	2.5
98	1.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.12910062406842193
3	0.0
4	2.76648146548712E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.005757047929678697
14-15	7.552494400779838E-4
16-17	0.0016958531383436046
18-19	0.0
20-21	2.213340945750626E-5
22-23	1.99237082470676E-4
24-25	5.121136894605147E-4
26-27	4.154773281916075E-5
28-29	0.009150501127845696
30-31	0.0026984373049740527
32-33	0.007414078304968036
34-35	0.006603746505040459
36-37	0.008863613006129133
38-39	0.0030441644846631185
40-41	2.8346319916339804E-5
42-43	2.1984663498743277E-4
44-45	0.0013736605513717944
46-47	7.274221688831026E-4
48-49	1.1452903941058776E-4
50-51	0.0
52-53	1.7836216622158864E-5
54-55	2.681521820839125E-5
56-57	0.0
58-59	0.001053875334631616
60-61	0.0
62-63	0.0
64-65	0.0
66-67	6.551538661612373E-4
68-69	0.001323513223207978
70-71	0.0014273637928499572
72-73	5.772789741506151E-4
74-75	1.8076362178781402E-4
76-77	0.003994036460522864
78-79	0.0020147056639889414
80-81	1.4040715540890658E-4
82-83	3.4780517025602184E-5
84-85	4.346007799635331E-4
86-87	0.0010527017338500554
88-89	1.0614059580962051E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	762.0
20-21	2680.0
22-23	5248.0
24-25	10233.0
26-27	12423.0
28-29	21892.0
30-31	42208.0
32-33	54405.0
34-35	76235.0
36-37	98617.0
38-39	78408.0
40-41	124860.0
42-43	142464.0
44-45	175406.0
46-47	164518.0
48-49	146845.0
50-51	87285.0
52-53	37880.0
54-55	39008.0
56-57	41275.0
58-59	105663.0
60-61	176357.0
62-63	171731.0
64-65	181885.0
66-67	195423.0
68-69	204576.0
70-71	207418.0
72-73	203152.0
74-75	209579.0
76-77	187405.0
78-79	211481.0
80-81	224268.0
82-83	224211.0
84-85	230355.0
86-87	249188.0
88-89	277288.0
90-91	310453.0
92-93	351651.0
94-95	423745.0
96-97	861277.0
98-99	1414340.0
100-101	1.0089401E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.32101242231411
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.16298072311135	71.69502850572376
2	7.597143473690235	12.356147975961324
3	2.1909858203366843	5.345195553381407
4	0.8802675773433773	2.863370023684045
5	0.43163650467036346	1.7550558779111431
6	0.22891817780122184	1.1169514788400001
7	0.14089547616144504	0.8020433937020912
8	0.09669292331128061	0.6290533134197214
9	0.06601769214703317	0.4831763006863252
>10	0.19936887529080732	2.516851699789906
>50	0.0038980859108203266	0.2088415418969455
>100	0.0011007051206880678	0.1490114429041955
>500	5.3728960874406E-5	0.03111931906231979
>1k	4.02361437941543E-5	0.04815357303677472
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.7664814654871202E-5	0.0	0.0	5.5329629309742406E-6
3	0.0	3.319777758584544E-5	0.0	0.0	5.5329629309742406E-6
4	0.0	7.192851810266512E-5	0.0	5.5329629309742406E-6	5.5329629309742406E-6
5	0.0	7.192851810266512E-5	0.0	5.5329629309742406E-6	1.1065925861948481E-5
6	0.0	8.29944439646136E-5	0.0	5.5329629309742406E-6	2.2131851723896962E-5
7	0.0	8.29944439646136E-5	0.0	1.1065925861948481E-5	2.2131851723896962E-5
8	0.0	9.406036982656208E-5	0.0	1.659888879292272E-5	2.2131851723896962E-5
9	0.0	1.7152185086020144E-4	0.0	3.319777758584544E-5	3.319777758584544E-5
10-11	0.0	1.7152185086020144E-4	0.0	3.596425905133256E-5	3.319777758584544E-5
12-13	0.0	1.7152185086020144E-4	0.0	3.873074051681968E-5	3.319777758584544E-5
14-15	0.0	2.4345036896286656E-4	0.0	4.14972219823068E-5	6.9162036637178E-5
16-17	0.0	2.655822206867635E-4	0.0	7.469499956815225E-5	9.959333275753632E-5
18-19	0.0	2.98779998272609E-4	0.0	1.0512629568851056E-4	1.244916659469204E-4
20-21	0.0	3.402772202549158E-4	0.0	1.5768944353276583E-4	1.38324073274356E-4
22-23	0.0	3.7347499784076124E-4	0.0	4.094392568920938E-4	1.6322240646374008E-4
24-25	0.0	4.315711086159907E-4	0.0	0.0012144853633488458	1.9088722111861129E-4
26-27	0.0	4.5923592327086195E-4	0.0	0.0034387364616004904	1.9918666551507264E-4
28-29	0.0	4.620024047363491E-4	0.0	0.012606856038224807	1.9918666551507264E-4
30-31	0.0	5.698951818903467E-4	0.0	0.018516060448505296	1.9918666551507264E-4
32-33	0.0	5.726616633558338E-4	0.0	0.031120150005264614	2.047196284460469E-4
34-35	0.0	6.445901814584991E-4	0.0	0.04412537937451956	2.1025259137702113E-4
36-37	0.0	7.082192551647028E-4	0.0	0.0690956410820063	2.1301907284250823E-4
38-39	0.0	7.303511068885997E-4	0.0	0.22023958946742964	2.1578555430799536E-4
40-41	0.0	7.801477732673679E-4	0.0	0.38066231668809675	2.185520357734825E-4
42-43	0.0	7.939801805948036E-4	0.0	0.43471936452371507	2.2961796163543097E-4
44-45	0.0	8.880405504213656E-4	0.0	0.5128309687017439	2.4068388749737946E-4
46-47	0.0	9.627355499895178E-4	0.0	0.5939331393439643	2.489833318938408E-4
48-49	0.0	9.682685129204921E-4	0.0	0.6684787489129802	2.5451629482481505E-4
50-51	0.0	0.0010678618456780283	0.0	0.7450909201367151	2.600492577557893E-4
52-53	0.0	0.0010678618456780283	0.0	1.2221706488599688	2.821811094796862E-4
54-55	0.0	0.0010733948086090025	0.0	1.8506239439302816	3.126124056000445E-4
56-57	0.0	0.0011121255491258223	0.0	2.5423107058572336	3.2367833146199304E-4
58-59	0.0	0.0011121255491258223	0.0	3.265419164269188	3.292112943929673E-4
60-61	0.0	0.0011370238823152064	0.0	3.4661744247751916	3.375107387894287E-4
62-63	2.7664814654871203E-6	0.0011591557340391032	0.0	3.4758626428673276	3.596425905133256E-4
64-65	5.5329629309742406E-6	0.0012781144370550496	0.0	3.483442802082762	3.67942034909787E-4
66-67	5.5329629309742406E-6	0.0012836473999860236	0.0	3.488950866680547	3.8454092370270965E-4
68-69	5.5329629309742406E-6	0.0013002462887789464	0.0	3.4947134475731567	3.9284036809917103E-4
70-71	5.5329629309742406E-6	0.0013168451775718692	0.0	3.4997595097662053	4.1773870128855513E-4
72-73	5.5329629309742406E-6	0.001336210547830279	0.0	3.5038788006683155	4.537029603398877E-4
74-75	5.5329629309742406E-6	0.001380474251278073	0.0	3.507549921573017	4.537029603398877E-4
76-77	5.5329629309742406E-6	0.0014413368435187896	0.0	3.5107728724803096	4.564694418053748E-4
78-79	5.5329629309742406E-6	0.001471768139639148	0.0	3.5130552196893365	4.5923592327086195E-4
80-81	5.5329629309742406E-6	0.001471768139639148	0.0	3.513879631166051	4.7583481206378466E-4
82-83	5.5329629309742406E-6	0.0014745346211046352	0.0	3.514502089495786	5.31164441373527E-4
84-85	5.5329629309742406E-6	0.0014800675840356092	0.0	3.5147704381979383	5.31164441373527E-4
86-87	8.299444396461361E-6	0.001493899991363045	0.0	3.5148949298638854	5.31164441373527E-4
88	1.1065925861948481E-5	0.00156582850946571	0.0	3.5149087622712125	5.31164441373527E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21460	0.0	52.56722	1
ATCTCGT	13885	0.0	45.94037	94
TATCTCG	16830	0.0	36.103073	94
GTATCAA	38770	0.0	35.763985	1
CCGTCTT	7420	0.0	31.491926	90-91
GCCGTCT	7965	0.0	31.258928	90-91
TATGCCG	7295	0.0	31.069223	86-87
ATGCCGT	7900	0.0	30.454725	88-89
TGCCGTC	8020	0.0	28.836582	88-89
TCAACGC	49140	0.0	27.673752	4
TCGTATG	8900	0.0	27.573399	84-85
ATCAACG	49580	0.0	27.411146	3
CAACGCA	50405	0.0	26.962494	5
TCTCGTA	8720	0.0	26.695358	82-83
CTCGTAT	8470	0.0	26.493502	82-83
TATCAAC	52060	0.0	26.317673	2
AACGCAG	51950	0.0	26.266178	6
CGTATGC	9070	0.0	25.735218	84-85
CGCTATC	25750	0.0	25.301062	94
CTATCTC	26915	0.0	23.559301	94
>>END_MODULE