FastQCFastQC Report
Fri 27 May 2016
SRR2139337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9554458
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115750.051.594121
GTATCAA214400.039.6113661
TCAACGC252050.032.8394854
ATCAACG254650.032.5041923
CAACGCA259700.031.8721315
TATCAAC268000.031.2067642
AACGCAG265450.031.1802716
ACGCAGA304150.027.2412287
CGCAGAG309000.026.7857728
GTACATG276950.023.7261051
TACATGG279400.022.8857672
GCAGAGT367350.022.6249399
ACATGGG279650.022.2488773
GAGTACT215000.021.00187512-13
CAGAGTA355900.019.87835310-11
AGAGTAC326350.019.55269610-11
CATGGGG208650.019.4943734
GTACTTT241150.018.67095614-15
GTGGTAT55100.017.4385831
AGTACTT238250.016.99022512-13
Produced by FastQC (version 0.11.5)