##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9554458
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.967546563080816	32.0	25.0	33.0	18.0	33.0
2	30.706899438984397	32.0	30.0	33.0	27.0	33.0
3	31.600385495440975	33.0	31.0	33.0	29.0	33.0
4	31.823600145607422	33.0	31.0	33.0	29.0	34.0
5	32.48374664475996	33.0	33.0	33.0	31.0	34.0
6	36.32484333491235	38.0	37.0	38.0	34.0	38.0
7	36.94943124978936	38.0	38.0	38.0	35.0	38.0
8	36.860842446531244	38.0	38.0	38.0	35.0	38.0
9	37.12066241748093	38.0	38.0	38.0	36.0	38.0
10-11	37.226272437431824	38.0	38.0	38.0	36.0	38.0
12-13	37.25120749915903	38.0	38.0	38.0	36.0	38.0
14-15	37.24983185859418	38.0	38.0	38.0	36.0	38.0
16-17	37.25199645024344	38.0	38.0	38.0	36.0	38.0
18-19	37.264486431360105	38.0	38.0	38.0	36.0	38.0
20-21	37.237940580555346	38.0	38.0	38.0	36.5	38.0
22-23	37.25646544289394	38.0	38.0	38.0	36.5	38.0
24-25	37.279061763327064	38.0	38.0	38.0	37.0	38.0
26-27	37.25520994725531	38.0	38.0	38.0	37.0	38.0
28-29	37.23384432665559	38.0	38.0	38.0	36.5	38.0
30-31	37.23267226432543	38.0	38.0	38.0	36.5	38.0
32-33	37.173072848130886	38.0	38.0	38.0	36.5	38.0
34-35	37.18128232728141	38.0	38.0	38.0	36.0	38.0
36-37	37.178613122189724	38.0	38.0	38.0	36.0	38.0
38-39	37.16688738487548	38.0	38.0	38.0	36.0	38.0
40-41	37.1603181416554	38.0	38.0	38.0	36.0	38.0
42-43	37.130043722221565	38.0	38.0	38.0	36.0	38.0
44-45	37.10354070208966	38.0	38.0	38.0	36.0	38.0
46-47	37.07517618356047	38.0	38.0	38.0	36.0	38.0
48-49	37.028128226478074	38.0	38.0	38.0	36.0	38.0
50-51	36.997797310481666	38.0	38.0	38.0	36.0	38.0
52-53	36.96140734609304	38.0	38.0	38.0	36.0	38.0
54-55	36.933299113796735	38.0	38.0	38.0	36.0	38.0
56-57	36.899535234041565	38.0	38.0	38.0	36.0	38.0
58-59	36.903843424940234	38.0	38.0	38.0	36.0	38.0
60-61	36.92853414047167	38.0	38.0	38.0	36.0	38.0
62-63	36.937941420136866	38.0	38.0	38.0	36.0	38.0
64-65	36.934723391237185	38.0	38.0	38.0	36.0	38.0
66-67	36.93805771445649	38.0	38.0	38.0	36.0	38.0
68-69	36.938816169752926	38.0	38.0	38.0	36.0	38.0
70-71	36.92292392278103	38.0	38.0	38.0	35.5	38.0
72-73	36.89799076040491	38.0	38.0	38.0	35.0	38.0
74-75	36.890452484836956	38.0	38.0	38.0	35.0	38.0
76-77	36.88756574475538	38.0	38.0	38.0	35.0	38.0
78-79	36.85779168878319	38.0	38.0	38.0	35.0	38.0
80-81	36.828410558148676	38.0	38.0	38.0	35.0	38.0
82-83	36.82291823775168	38.0	38.0	38.0	35.0	38.0
84-85	36.77411980818957	38.0	38.0	38.0	35.0	38.0
86-87	36.75482034525879	38.0	38.0	38.0	35.0	38.0
88-89	36.75630074893075	38.0	38.0	38.0	35.0	38.0
90-91	36.73163048710282	38.0	38.0	38.0	35.0	38.0
92-93	36.73787959344983	38.0	38.0	38.0	35.0	38.0
94-95	36.68144005834694	38.0	38.0	38.0	34.5	38.0
96-97	36.64928299449905	38.0	38.0	38.0	34.0	38.0
98-99	36.73834141648308	38.0	38.0	38.0	34.0	38.0
100	35.080057049742614	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	4.0
19	31.0
20	125.0
21	374.0
22	1071.0
23	2684.0
24	6046.0
25	11525.0
26	19772.0
27	31840.0
28	47660.0
29	67216.0
30	92635.0
31	122577.0
32	160326.0
33	213849.0
34	310936.0
35	535896.0
36	1458758.0
37	6471130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24176651360025	18.765690319639273	13.633614800546509	23.35892836621397
2	14.382525312808307	20.484048257456834	42.853664974785055	22.279761454949806
3	17.438529741823135	27.096858869440844	30.725751267104844	24.738860121631177
4	11.97168902725827	18.50673266866629	39.454378259865706	30.06720004420973
5	13.129923501513893	37.71280746764556	36.07038430106375	13.086884729776802
6	29.351089534652246	38.55074381078715	19.10238437686497	12.99578227769564
7	25.97808269187012	33.1070061744999	23.68652413355106	17.228387000078914
8	24.307668734322764	36.74259701596888	21.391260498502373	17.55847375120598
9	25.806665328373413	16.571185932263244	21.192295784857706	36.429852954505634
10-11	23.95752328389533	26.680885509151853	29.45316730682159	19.90842390013123
12-13	24.885252517725235	24.39239358213726	29.397774316449976	21.32457958368753
14-15	22.162633401078324	25.665537490457336	26.862795356889947	25.309033751574393
16-17	21.648412709543546	28.806992505488015	27.48017731618057	22.06441746878787
18-19	21.69549020991039	27.92474989162127	29.228062962859852	21.151696935608488
20-21	22.57685057271801	27.09881558698951	29.03563433337663	21.288699506915854
22-23	22.304028957431562	26.990980424091305	28.94057978083473	21.7644108376424
24-25	22.034467822209695	27.13538645191289	29.012177354843043	21.817968371034368
26-27	21.970957286845394	27.272342777255904	28.92534067750139	21.831359258397317
28-29	21.93132762253881	27.262336298481067	28.97000335325388	21.836332725726244
30-31	22.229056791374	27.12529391646248	28.846641037818465	21.799008254345058
32-33	22.026557832071994	27.318321502173603	28.8189306255583	21.836190040196108
34-35	22.169774858770722	27.313568855695692	28.795838902986194	21.720817382547395
36-37	22.130655032180975	27.209625640160073	28.803904892630396	21.855814435028563
38-39	21.94428000234717	27.349150122329714	28.864623024258705	21.84194685106441
40-41	22.181702712491752	27.1489998136476	28.791835193416237	21.87746228044441
42-43	22.087620446419695	27.25994183087303	28.61123583401063	22.041201888696644
44-45	22.130478092759496	27.34789522587711	28.518531619879354	22.00309506148404
46-47	22.22315349034151	27.301408131821127	28.328588401321653	22.146849976515718
48-49	22.110700647219637	27.409309974788087	28.302105957817663	22.177883420174606
50-51	22.105429840203136	27.5689342282603	28.18935968653909	22.136276244997475
52-53	22.323243643389663	27.452480978069683	28.073674471583693	22.15060090695696
54-55	22.248196125568438	27.343337699389924	28.13292271464033	22.275543460401313
56-57	22.253304910974006	27.43897252460797	28.123158696886268	22.184563867531754
58-59	22.203247814870554	27.449568607000135	28.15499712222961	22.1921864558997
60-61	22.264753307655212	27.46178311198939	28.13969513015479	22.133768450200602
62-63	22.184400500432357	27.567387241973577	28.102777203512712	22.145435054081354
64-65	22.239310974085978	27.49585340764461	28.050921432681076	22.21391418558834
66-67	22.263377114080352	27.55178625213444	27.910419599023516	22.274417034761687
68-69	22.202330849743074	27.518384229857134	27.975381943420068	22.30390297697972
70-71	22.253940043174108	27.29735403437316	28.032027560013635	22.4166783624391
72-73	22.336532693461308	27.3054907090871	27.920343642114954	22.437632955336642
74-75	22.177572296189922	27.483765780147664	27.92971630662842	22.408945617033996
76-77	22.409472086027783	27.37681942973528	27.80516781681545	22.408540667421484
78-79	22.334171296989496	27.465225188585112	27.702247250425955	22.498356263999433
80-81	22.359612847233173	27.465258182921332	27.673299746522133	22.501829223323362
82-83	22.401762592624795	27.416700026652258	27.74124975827278	22.440287622450168
84-85	22.477713277139866	27.404200770999797	27.69236956597964	22.425716385880694
86-87	22.367324174651205	27.43695966759723	27.77488698506176	22.420829172689803
88-89	22.408673331164085	27.51618539637021	27.649602006741446	22.42553926572426
90-91	22.476820932584953	27.494249662435678	27.635093376361958	22.393836028617407
92-93	22.563230306556356	27.479432947844494	27.62724306520999	22.330093680389158
94-95	22.452731067820622	27.530275377276336	27.62244766915785	22.394545885745192
96-97	22.532408474235506	27.618095149740498	27.752894106879882	22.09660226914412
98-99	23.562662688958	29.558602927804223	27.942281585768423	18.936452797469354
100	22.47629635408136	30.329757830742277	21.973754507709764	25.2201913074666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1542.0
1	1533.0
2	2378.0
3	3499.0
4	4424.5
5	6276.5
6	7979.0
7	9785.0
8	12242.0
9	13733.5
10	13240.0
11	12506.0
12	13655.0
13	16368.0
14	21180.0
15	26791.5
16	31075.0
17	33868.0
18	34312.5
19	32469.5
20	30284.5
21	29004.5
22	30190.0
23	35086.0
24	43532.5
25	56214.0
26	73581.0
27	93149.0
28	113812.0
29	138009.0
30	161939.5
31	185424.5
32	210873.5
33	233307.5
34	253156.5
35	272531.5
36	292772.5
37	312115.5
38	326496.5
39	336408.0
40	347352.5
41	358530.5
42	365935.0
43	373827.0
44	381752.0
45	390233.0
46	393824.5
47	392319.0
48	390055.0
49	383128.0
50	373900.0
51	362885.0
52	348982.5
53	331933.5
54	313713.5
55	293287.0
56	271135.0
57	247740.0
58	221783.0
59	193936.0
60	162854.0
61	132008.5
62	104488.5
63	80551.0
64	60527.5
65	44518.5
66	32721.0
67	24829.0
68	18925.5
69	13855.0
70	10081.0
71	7314.0
72	5205.0
73	3633.5
74	2401.5
75	1539.0
76	940.0
77	525.5
78	324.0
79	199.5
80	127.0
81	88.5
82	64.0
83	43.5
84	28.5
85	20.5
86	16.5
87	14.0
88	9.5
89	5.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002135128962835987
3	0.0
4	0.0
5	0.0029515018015673937
6	1.2559582134329337E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.901569967833224E-5
44-45	1.620168024385473E-5
46-47	0.0
48-49	0.0
50-51	2.210023140047288E-5
52-53	0.0011123405564094314
54-55	0.002088094758523877
56-57	0.0024294112302155175
58-59	0.0016182937834635473
60-61	0.003965612588147613
62-63	0.0015108469294659959
64-65	0.0014877531185281288
66-67	2.2853286762632112E-4
68-69	2.4831308212050626E-4
70-71	2.0288543668046965E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	8.040090613058146E-5
80-81	3.247223826579763E-4
82-83	6.305241799717777E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	5.896602678918322E-5
92-93	2.6484451872156107E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	54.0
20-21	334.0
22-23	771.0
24-25	855.0
26-27	2284.0
28-29	5934.0
30-31	15657.0
32-33	20496.0
34-35	27527.0
36-37	43670.0
38-39	52171.0
40-41	51500.0
42-43	58019.0
44-45	70092.0
46-47	70844.0
48-49	68516.0
50-51	61580.0
52-53	57469.0
54-55	61251.0
56-57	63179.0
58-59	67099.0
60-61	67983.0
62-63	66625.0
64-65	69514.0
66-67	74646.0
68-69	77264.0
70-71	79418.0
72-73	71232.0
74-75	70678.0
76-77	74087.0
78-79	77203.0
80-81	78055.0
82-83	73336.0
84-85	71869.0
86-87	73268.0
88-89	78213.0
90-91	80183.0
92-93	80724.0
94-95	96836.0
96-97	298859.0
98-99	660077.0
100-101	6435086.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.35782770237611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1233418074285	74.29128170643598
2	7.310213906070715	12.187271024995129
3	1.9217008249838523	4.80566418793554
4	0.7137546190287904	2.3798813821910807
5	0.35295674428042095	1.4710853738059477
6	0.19171713727479137	0.9588674457926908
7	0.10843118145070416	0.6327011418653012
8	0.07008246528849742	0.46735376531810574
9	0.045238710789064544	0.33939005934892186
>10	0.15746435588586427	2.0314888080370457
>50	0.004079609087926097	0.2265365523031157
>100	8.83411920075641E-4	0.11099353692188331
>500	9.015175452651542E-5	0.04798214270250946
>1k	4.50747562412355E-5	0.04950287234677436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.2331592226372235E-5	0.0	0.0	0.0
3	0.0	5.2331592226372235E-5	0.0	0.0	0.0
4	0.0	7.326422911692112E-5	0.0	2.0932636890548894E-5	0.0
5	0.0	7.326422911692112E-5	0.0	2.0932636890548894E-5	0.0
6	0.0	7.326422911692112E-5	0.0	3.139895533582334E-5	0.0
7	0.0	7.326422911692112E-5	0.0	3.139895533582334E-5	0.0
8	0.0	8.373054756219558E-5	0.0	3.139895533582334E-5	0.0
9	0.0	1.8839373201494003E-4	0.0	3.139895533582334E-5	0.0
10-11	1.0466318445274447E-5	1.8839373201494003E-4	0.0	3.139895533582334E-5	0.0
12-13	1.0466318445274447E-5	1.8839373201494003E-4	0.0	3.139895533582334E-5	0.0
14-15	1.569947766791167E-5	2.5119164268658673E-4	0.0	3.139895533582334E-5	0.0
16-17	2.0932636890548894E-5	2.5119164268658673E-4	0.0	3.663211455846056E-5	0.0
18-19	2.0932636890548894E-5	2.564248019092239E-4	0.0	4.186527378109779E-5	0.0
20-21	2.0932636890548894E-5	2.6165796113186116E-4	0.0	5.2331592226372235E-5	0.0
22-23	2.0932636890548894E-5	3.0352323491295897E-4	0.0	5.7564751449009456E-5	5.2331592226372235E-6
24-25	2.6165796113186118E-5	3.506216679166939E-4	0.0	8.373054756219558E-5	1.0466318445274447E-5
26-27	3.139895533582334E-5	3.7678746402988007E-4	0.0	1.3606213978856782E-4	1.0466318445274447E-5
28-29	3.139895533582334E-5	3.7678746402988007E-4	0.0	3.9248694169779174E-4	1.569947766791167E-5
30-31	3.663211455846056E-5	4.291190562562523E-4	0.0	0.001266424531878208	2.0932636890548894E-5
32-33	4.186527378109779E-5	4.291190562562523E-4	0.0	0.003275957673370902	2.0932636890548894E-5
34-35	4.7098433003735015E-5	5.023832853731735E-4	0.0	0.006133262608930826	2.0932636890548894E-5
36-37	5.2331592226372235E-5	5.756475144900946E-4	0.0	0.010403520534602799	2.0932636890548894E-5
38-39	5.2331592226372235E-5	5.913469921580062E-4	0.0	0.023439320158192126	2.0932636890548894E-5
40-41	6.279791067164668E-5	6.593780620522901E-4	0.0	0.03807646650390843	2.0932636890548894E-5
42-43	6.279791067164668E-5	6.855438581654762E-4	0.0	0.046161697502882945	2.0932636890548894E-5
44-45	6.803106989428391E-5	7.117096542786623E-4	0.0	0.056371591146248165	2.0932636890548894E-5
46-47	7.326422911692112E-5	7.221759727239368E-4	0.0	0.06696350541286591	2.0932636890548894E-5
48-49	7.326422911692112E-5	7.221759727239368E-4	0.0	0.0775187875649252	2.0932636890548894E-5
50-51	7.326422911692112E-5	8.059065202861324E-4	0.0	0.08858168616158027	2.0932636890548894E-5
52-53	8.373054756219558E-5	8.059065202861324E-4	0.0	0.12249779108349212	2.6165796113186118E-5
54-55	8.373054756219558E-5	8.111396795087696E-4	0.0	0.16346819463751894	3.139895533582334E-5
56-57	8.373054756219558E-5	8.268391571766813E-4	0.0	0.21848439754510407	3.663211455846056E-5
58-59	8.373054756219558E-5	8.477717940672301E-4	0.0	0.2551217452627873	4.186527378109779E-5
60-61	8.373054756219558E-5	8.739375901804162E-4	0.0	0.27106718141416286	4.186527378109779E-5
62-63	8.373054756219558E-5	9.315023416294258E-4	0.0	0.2857043277598792	4.186527378109779E-5
64-65	8.373054756219558E-5	0.0010884971183085426	0.0	0.29286852273566955	4.186527378109779E-5
66-67	8.373054756219558E-5	0.0010884971183085426	0.0	0.29878722581647227	5.2331592226372235E-5
68-69	8.373054756219558E-5	0.0011146629144217286	0.0	0.30507748320208217	6.279791067164668E-5
70-71	8.896370678483279E-5	0.0011408287105349147	0.0	0.31013271501114975	7.326422911692112E-5
72-73	9.419686600747002E-5	0.0011408287105349147	0.0	0.3139162891291165	7.326422911692112E-5
74-75	9.419686600747002E-5	0.001198393461983924	0.0	0.316909656204465	7.326422911692112E-5
76-77	9.419686600747002E-5	0.0012245592580971102	0.0	0.3198035932545834	7.326422911692112E-5
78-79	1.0466318445274447E-4	0.0012350255765423846	0.0	0.3216456653009517	7.326422911692112E-5
80-81	1.0466318445274447E-4	0.0012350255765423846	0.0	0.3222108464969965	7.326422911692112E-5
82-83	1.0466318445274447E-4	0.0012350255765423846	0.0	0.3226190329163622	7.326422911692112E-5
84-85	1.0466318445274447E-4	0.0012350255765423846	0.0	0.3228178929668224	7.849738833955835E-5
86-87	1.0466318445274447E-4	0.0012350255765423846	0.0	0.3229173229920525	8.373054756219558E-5
88	1.0466318445274447E-4	0.001287357168768757	0.0	0.32293825562894307	9.419686600747002E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11575	0.0	51.59412	1
GTATCAA	21440	0.0	39.611366	1
TCAACGC	25205	0.0	32.839485	4
ATCAACG	25465	0.0	32.504192	3
CAACGCA	25970	0.0	31.872131	5
TATCAAC	26800	0.0	31.206764	2
AACGCAG	26545	0.0	31.180271	6
ACGCAGA	30415	0.0	27.241228	7
CGCAGAG	30900	0.0	26.785772	8
GTACATG	27695	0.0	23.726105	1
TACATGG	27940	0.0	22.885767	2
GCAGAGT	36735	0.0	22.624939	9
ACATGGG	27965	0.0	22.248877	3
GAGTACT	21500	0.0	21.001875	12-13
CAGAGTA	35590	0.0	19.878353	10-11
AGAGTAC	32635	0.0	19.552696	10-11
CATGGGG	20865	0.0	19.494373	4
GTACTTT	24115	0.0	18.670956	14-15
GTGGTAT	5510	0.0	17.438583	1
AGTACTT	23825	0.0	16.990225	12-13
>>END_MODULE