FastQCFastQC Report
Fri 27 May 2016
SRR2139336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7312676
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93850.053.6475141
GTATCAA176350.038.6753541
TCAACGC209700.031.6433244
ATCAACG209900.031.5518263
CAACGCA213000.031.1530765
AACGCAG219400.030.3021856
TATCAAC221300.030.2378252
ACGCAGA249150.026.6666977
CGCAGAG255450.025.9586288
GTACATG231450.023.886061
TACATGG233650.022.9447082
ACATGGG231650.022.4564633
GCAGAGT305200.021.8256079
GAGTACT169550.020.9979912-13
CATGGGG181600.020.1606374
CAGAGTA295050.019.62345510-11
AGAGTAC268950.019.57289910-11
GTGGTAT42350.019.1555351
GTACTTT194100.018.26499714-15
AGTACTT188200.017.1839412-13
Produced by FastQC (version 0.11.5)