##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139336_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7312676
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.809461406467346	32.0	25.0	33.0	18.0	33.0
2	30.62693834650954	32.0	30.0	33.0	25.0	33.0
3	31.55542745227602	33.0	31.0	33.0	29.0	33.0
4	31.771548746313936	33.0	31.0	33.0	29.0	34.0
5	32.47020598205089	33.0	33.0	33.0	31.0	34.0
6	36.312440890311564	38.0	37.0	38.0	34.0	38.0
7	36.93385513046113	38.0	37.0	38.0	35.0	38.0
8	36.844794162902886	38.0	38.0	38.0	35.0	38.0
9	37.10772390298709	38.0	38.0	38.0	36.0	38.0
10-11	37.212072639345706	38.0	38.0	38.0	36.0	38.0
12-13	37.240008650731966	38.0	38.0	38.0	36.0	38.0
14-15	37.23614091476226	38.0	38.0	38.0	36.0	38.0
16-17	37.236823906870754	38.0	38.0	38.0	36.0	38.0
18-19	37.24938271571173	38.0	38.0	38.0	36.0	38.0
20-21	37.22381910102137	38.0	38.0	38.0	36.0	38.0
22-23	37.24454370575751	38.0	38.0	38.0	36.0	38.0
24-25	37.267802090380655	38.0	38.0	38.0	37.0	38.0
26-27	37.24555772129933	38.0	38.0	38.0	36.5	38.0
28-29	37.22543387936306	38.0	38.0	38.0	36.0	38.0
30-31	37.22373262234895	38.0	38.0	38.0	36.0	38.0
32-33	37.164420096069875	38.0	38.0	38.0	36.0	38.0
34-35	37.17478779752628	38.0	38.0	38.0	36.0	38.0
36-37	37.17488709639428	38.0	38.0	38.0	36.0	38.0
38-39	37.163132837010394	38.0	38.0	38.0	36.0	38.0
40-41	37.155697847062086	38.0	38.0	38.0	36.0	38.0
42-43	37.125544729800744	38.0	38.0	38.0	36.0	38.0
44-45	37.0971554241234	38.0	38.0	38.0	36.0	38.0
46-47	37.064503789478	38.0	38.0	38.0	36.0	38.0
48-49	37.015269284495936	38.0	38.0	38.0	36.0	38.0
50-51	36.981279250762505	38.0	38.0	38.0	36.0	38.0
52-53	36.94459960677362	38.0	38.0	38.0	36.0	38.0
54-55	36.91451746386501	38.0	38.0	38.0	36.0	38.0
56-57	36.88066856647582	38.0	38.0	38.0	35.5	38.0
58-59	36.883523142596516	38.0	38.0	38.0	35.0	38.0
60-61	36.91056127459531	38.0	38.0	38.0	36.0	38.0
62-63	36.91890963804752	38.0	38.0	38.0	36.0	38.0
64-65	36.916794486571746	38.0	38.0	38.0	35.5	38.0
66-67	36.91995499661149	38.0	38.0	38.0	35.5	38.0
68-69	36.92166203861636	38.0	38.0	38.0	35.5	38.0
70-71	36.90571578276703	38.0	38.0	38.0	35.0	38.0
72-73	36.878381142584416	38.0	38.0	38.0	35.0	38.0
74-75	36.87147662555866	38.0	38.0	38.0	35.0	38.0
76-77	36.86863967467037	38.0	38.0	38.0	35.0	38.0
78-79	36.83690671289669	38.0	38.0	38.0	35.0	38.0
80-81	36.805703176575236	38.0	38.0	38.0	35.0	38.0
82-83	36.80277182587777	38.0	38.0	38.0	35.0	38.0
84-85	36.74744741181297	38.0	38.0	38.0	35.0	38.0
86-87	36.72871133514866	38.0	38.0	38.0	35.0	38.0
88-89	36.73044952260145	38.0	38.0	38.0	35.0	38.0
90-91	36.7047548222026	38.0	38.0	38.0	34.0	38.0
92-93	36.711532442175354	38.0	38.0	38.0	34.0	38.0
94-95	36.65300201936568	38.0	38.0	38.0	34.0	38.0
96-97	36.620641017446864	38.0	38.0	38.0	34.0	38.0
98-99	36.71224305549836	38.0	38.0	38.0	34.0	38.0
100	35.04856278596764	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	8.0
19	28.0
20	104.0
21	322.0
22	871.0
23	2149.0
24	4847.0
25	9012.0
26	15003.0
27	24098.0
28	35650.0
29	51456.0
30	70816.0
31	94178.0
32	123993.0
33	165975.0
34	244203.0
35	423219.0
36	1155577.0
37	4891166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45905985715763	18.734277301496743	13.698801916015423	23.107860925330208
2	14.553730781375407	20.286966036116496	42.79251577742942	22.36678740507867
3	17.51583141383537	27.14329200418561	30.537138524939433	24.803738057039585
4	12.143817119752057	18.327681959381216	39.002890323597	30.525610597269726
5	13.272637886130054	37.672139285452985	35.837272383525935	13.217950444891018
6	29.70398765539472	38.26303887992727	18.86366234594542	13.169311118732589
7	26.28965101147651	33.00277490757145	23.460741320961027	17.24683275999101
8	24.619222839901564	36.69725009011749	21.045018266910773	17.638508803070177
9	25.969371540596082	16.5394583323533	21.100046002311604	36.391124124739015
10-11	24.30984908944414	26.54888579775721	29.061269773199307	20.07999533959935
12-13	25.22693470899025	24.28340186273807	29.093638224912468	21.39602520335921
14-15	22.478214541434628	25.43429382075727	26.57287154524554	25.514620092562556
16-17	21.906713766615667	28.59314428808278	27.203687131769545	22.29645481353201
18-19	21.970213092990857	27.718300386889833	28.967084005909737	21.344402514209573
20-21	22.789431266260102	26.835204779934056	28.82043719379351	21.554926760012332
22-23	22.652302760172237	26.70049884640956	28.726073879158044	21.921124514260164
24-25	22.319256242712704	26.89591432703848	28.768724718851402	22.016104711397418
26-27	22.242055082535988	26.97556411200078	28.724019441249094	22.05836136421414
28-29	22.234565921282705	26.929275234125843	28.775550362337782	22.060608482253674
30-31	22.454054919537683	26.813298102670906	28.605155727074816	22.127491250716595
32-33	22.243258735506284	27.026921149322693	28.638135419085785	22.091684696085235
34-35	22.397741958022337	27.001306884981467	28.61694378716618	21.98400736983002
36-37	22.409531137325803	26.875157420409966	28.599898560875552	22.11541288138868
38-39	22.244207885276317	27.03574141891525	28.675850149105525	22.04420054670291
40-41	22.39959499470161	26.837454785060018	28.631805195577204	22.131145024661173
42-43	22.342317935386244	26.97748684981185	28.38629144866663	22.29390376613528
44-45	22.360627114904204	27.05244743333551	28.325824903636203	22.261100548124084
46-47	22.523786670486075	27.015586526474504	28.084884878037307	22.37574192500211
48-49	22.458864303373556	27.161015643135546	27.952925535984903	22.427194517506
50-51	22.38795183410135	27.291238114907657	27.861007077503785	22.459802973487207
52-53	22.630595617494865	27.145007522682736	27.760117842356458	22.46427901746594
54-55	22.559064376619524	27.04722697116152	27.799016826350005	22.59469182586895
56-57	22.51563933926728	27.165425204976028	27.855437729469894	22.463497726286793
58-59	22.558157815291104	27.11610561749218	27.830329111990675	22.495407455226044
60-61	22.58913124765547	27.178995297252563	27.841532783411	22.390340671680963
62-63	22.545306016111823	27.27598419283294	27.723503626323776	22.45520616473146
64-65	22.55735907044093	27.226946304046333	27.683337756427346	22.532356869085383
66-67	22.616826378463028	27.209992852269732	27.60275951268377	22.57042125658347
68-69	22.481090412359542	27.207761714100965	27.686818866751455	22.624329006788038
70-71	22.62982977087475	26.964076900297645	27.690158619611893	22.71593470921571
72-73	22.671469696738917	27.024921747673375	27.5440097939165	22.75959876167121
74-75	22.45188479310037	27.243946156178588	27.589395235178493	22.714773815542554
76-77	22.768573965889846	27.041277167095295	27.525147066497336	22.66500180051752
78-79	22.592303667761033	27.118122514791466	27.426907190472132	22.862666626975372
80-81	22.70751173120947	27.14521783895269	27.36604109004836	22.78122933978948
82-83	22.718025457456882	27.09278305898959	27.436293937583123	22.752897545970402
84-85	22.72356905511444	27.100676289695834	27.39601368460457	22.77974097058516
86-87	22.662359363760977	27.160488172305687	27.51679491877367	22.66035754515967
88-89	22.690866456168408	27.145203106090076	27.37581340911659	22.788117028624924
90-91	22.77198699466078	27.107654297680938	27.414887900609347	22.705470807048933
92-93	22.79986778538328	27.07577604945352	27.388573067182097	22.7357830979811
94-95	22.70906689770466	27.09401726814701	27.392059465649943	22.804856368498392
96-97	22.828684746134364	27.20018730150186	27.438220794157285	22.532907158206488
98-99	23.866589454237722	29.182260444450154	27.563572312416902	19.38757778889523
100	22.79695720539564	29.829041621065677	21.57292092459025	25.801080248948434
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1909.0
1	1802.0
2	2343.5
3	3138.0
4	3768.5
5	5193.5
6	6465.5
7	8047.5
8	10352.5
9	11482.0
10	10757.0
11	10008.5
12	10967.0
13	13208.0
14	17020.0
15	21554.5
16	25236.0
17	27571.0
18	27693.0
19	25655.0
20	22882.5
21	20999.0
22	21147.0
23	24203.0
24	30503.5
25	39948.5
26	52422.0
27	66914.0
28	83947.5
29	104386.5
30	124275.5
31	143739.0
32	164189.0
33	181859.0
34	197218.5
35	210834.0
36	225264.0
37	240340.5
38	250132.5
39	253198.5
40	255312.5
41	258517.0
42	260575.5
43	264843.0
44	269879.5
45	275488.5
46	280067.0
47	282728.0
48	284953.0
49	284184.0
50	282659.0
51	280923.5
52	276111.0
53	267575.0
54	257141.0
55	244641.5
56	230233.0
57	212627.0
58	191537.5
59	167801.0
60	140976.5
61	114747.0
62	91191.0
63	70895.5
64	53652.5
65	39422.5
66	29186.5
67	22422.5
68	17177.5
69	12620.5
70	9178.0
71	6677.5
72	4759.5
73	3227.0
74	2116.0
75	1362.5
76	836.0
77	496.5
78	321.0
79	197.5
80	126.0
81	77.0
82	54.0
83	42.0
84	29.5
85	24.0
86	16.5
87	13.0
88	9.5
89	5.0
90	3.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002365755025930316
3	0.0
4	0.0
5	0.002885400638562409
6	1.0939907634359842E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	8.422289673844727E-5
44-45	2.1202928350569783E-5
46-47	0.0
48-49	0.0
50-51	2.1724790498793222E-5
52-53	0.0011887951029226711
54-55	0.0022693531205257847
56-57	0.0027006066080366205
58-59	0.0019017158921475017
60-61	0.004316606707946662
62-63	0.0018424522842768912
64-65	0.0017885693906068922
66-67	2.467121177205373E-4
68-69	2.0231398959997155E-4
70-71	2.671375201168303E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	1.1418030489240586E-4
80-81	4.8612023730906903E-4
82-83	0.0
84-85	8.402679244693247E-6
86-87	0.0
88-89	0.0
90-91	4.329915298196937E-5
92-93	3.8525965055723866E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	54.0
20-21	304.0
22-23	655.0
24-25	789.0
26-27	2034.0
28-29	4849.0
30-31	12388.0
32-33	15961.0
34-35	22042.0
36-37	34731.0
38-39	42223.0
40-41	41660.0
42-43	46848.0
44-45	56541.0
46-47	58168.0
48-49	55984.0
50-51	50223.0
52-53	46902.0
54-55	49815.0
56-57	52466.0
58-59	55069.0
60-61	55349.0
62-63	52689.0
64-65	55035.0
66-67	58821.0
68-69	61565.0
70-71	62479.0
72-73	56810.0
74-75	56122.0
76-77	57984.0
78-79	62019.0
80-81	61656.0
82-83	58097.0
84-85	55685.0
86-87	57095.0
88-89	61456.0
90-91	64005.0
92-93	63241.0
94-95	76144.0
96-97	228428.0
98-99	504594.0
100-101	4853696.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.91953174090956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.58975885006804	73.45821320887738
2	7.643724630266124	12.676281341962481
3	2.0242947992022002	5.0356073056621495
4	0.7741020657584209	2.5675272324943617
5	0.37538628824591525	1.5563427621654695
6	0.1854460187493479	0.9226258222747117
7	0.11469806326666289	0.6657496788362633
8	0.07625551963191736	0.5058457584430658
9	0.05458259993172791	0.40733672647862523
>10	0.15856183993155165	1.9838970163763439
>50	0.0027665804845830675	0.1470165886193759
>100	3.9372868845735735E-4	0.049544932487230485
>500	2.9015775061721227E-5	0.0240116253225505
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.469953817179922E-5	0.0	0.0	0.0
5	0.0	8.204930725769882E-5	0.0	0.0	0.0
6	4.102465362884941E-5	8.204930725769882E-5	0.0	0.0	1.3674884542949804E-5
7	4.102465362884941E-5	8.204930725769882E-5	0.0	0.0	1.3674884542949804E-5
8	4.102465362884941E-5	8.204930725769882E-5	0.0	0.0	1.3674884542949804E-5
9	4.102465362884941E-5	1.6409861451539763E-4	0.0	1.3674884542949804E-5	1.3674884542949804E-5
10-11	4.102465362884941E-5	1.6409861451539763E-4	0.0	1.3674884542949804E-5	1.3674884542949804E-5
12-13	4.102465362884941E-5	1.6409861451539763E-4	0.0	1.3674884542949804E-5	2.7349769085899606E-5
14-15	4.102465362884941E-5	2.461479217730965E-4	0.0	1.3674884542949804E-5	4.786209590032431E-5
16-17	1.2307396088654825E-4	2.5982280631604626E-4	0.0	1.3674884542949804E-5	5.469953817179922E-5
18-19	1.2307396088654825E-4	2.5982280631604626E-4	0.0	1.3674884542949804E-5	6.837442271474902E-5
20-21	1.2307396088654825E-4	2.5982280631604626E-4	0.0	1.3674884542949804E-5	6.837442271474902E-5
22-23	1.2307396088654825E-4	3.076849022163706E-4	0.0	2.0512326814424708E-5	7.521186498622392E-5
24-25	1.2307396088654825E-4	3.692218826596447E-4	0.0	3.418721135737451E-5	8.888674952917371E-5
26-27	1.2307396088654825E-4	3.828967672025945E-4	0.0	9.572419180064863E-5	1.0256163407212354E-4
28-29	1.2307396088654825E-4	3.897342094740694E-4	0.0	3.8973420947406947E-4	1.0939907634359844E-4
30-31	1.2307396088654825E-4	4.3759630537439374E-4	0.0	0.0012307396088654823	1.0939907634359844E-4
32-33	1.2307396088654825E-4	4.3759630537439374E-4	0.0	0.002858050869476509	1.0939907634359844E-4
34-35	1.2307396088654825E-4	5.401579394465172E-4	0.0	0.004950308204547829	1.0939907634359844E-4
36-37	1.2307396088654825E-4	6.29044688975691E-4	0.0	0.00838270422482823	1.0939907634359844E-4
38-39	1.2307396088654825E-4	6.769067848760152E-4	0.0	0.018064522481236692	1.0939907634359844E-4
40-41	1.2307396088654825E-4	7.999807457625635E-4	0.0	0.028799306847452286	1.2307396088654825E-4
42-43	1.2307396088654825E-4	8.478428416628878E-4	0.0	0.034686344643192174	1.2991140315802316E-4
44-45	1.2307396088654825E-4	9.572419180064862E-4	0.0	0.04209813206547097	1.4358628770097293E-4
46-47	1.2307396088654825E-4	0.0010119414561782854	0.0	0.04993384090858121	1.5726117224392275E-4
48-49	1.2307396088654825E-4	0.0010119414561782854	0.0	0.0575097269453754	1.7777349905834746E-4
50-51	1.3674884542949804E-4	0.0011623651861507333	0.0	0.06610439188061934	1.7777349905834746E-4
52-53	1.3674884542949804E-4	0.0011623651861507333	0.0	0.08671928032911619	1.7777349905834746E-4
54-55	1.3674884542949804E-4	0.001182877512965158	0.0	0.11073237758653603	1.7777349905834746E-4
56-57	1.3674884542949804E-4	0.0012170647243225326	0.0	0.1410085719646269	1.7777349905834746E-4
58-59	1.3674884542949804E-4	0.0012170647243225326	0.0	0.16539772854697787	1.7777349905834746E-4
60-61	1.3674884542949804E-4	0.0012307396088654823	0.0	0.17444366467213918	1.7777349905834746E-4
62-63	1.3674884542949804E-4	0.001244414493408432	0.0	0.18647756306993502	1.7777349905834746E-4
64-65	1.3674884542949804E-4	0.0013538135697520306	0.0	0.1918996547912146	1.7777349905834746E-4
66-67	1.3674884542949804E-4	0.0013674884542949804	0.0	0.19716448534025027	1.7777349905834746E-4
68-69	1.3674884542949804E-4	0.0014085131079238297	0.0	0.20288058707920328	1.7777349905834746E-4
70-71	1.5726117224392275E-4	0.0014427003192812042	0.0	0.20809071809006716	1.8461094132982234E-4
72-73	1.7777349905834746E-4	0.0014700500883671039	0.0	0.21174874970530622	2.0512326814424705E-4
74-75	1.7777349905834746E-4	0.0015042372997244784	0.0	0.21479141151611258	2.0512326814424705E-4
76-77	1.7777349905834746E-4	0.001593124049253652	0.0	0.2176221126165032	2.0512326814424705E-4
78-79	1.7777349905834746E-4	0.0016409861451539764	0.0	0.21948189691434433	2.0512326814424705E-4
80-81	1.7777349905834746E-4	0.0016409861451539764	0.0	0.22002205485379087	2.0512326814424705E-4
82-83	1.7777349905834746E-4	0.0016614984719684012	0.0	0.22052802558188	2.0512326814424705E-4
84-85	1.7777349905834746E-4	0.0016956856833257757	0.0	0.2207194739654813	2.1879815268719687E-4
86-87	1.7777349905834746E-4	0.0017230354524116753	0.0	0.22082203559955343	2.2563559495867176E-4
88	1.7777349905834746E-4	0.001818759644212324	0.0	0.22082203559955343	2.3247303723014667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9385	0.0	53.647514	1
GTATCAA	17635	0.0	38.675354	1
TCAACGC	20970	0.0	31.643324	4
ATCAACG	20990	0.0	31.551826	3
CAACGCA	21300	0.0	31.153076	5
AACGCAG	21940	0.0	30.302185	6
TATCAAC	22130	0.0	30.237825	2
ACGCAGA	24915	0.0	26.666697	7
CGCAGAG	25545	0.0	25.958628	8
GTACATG	23145	0.0	23.88606	1
TACATGG	23365	0.0	22.944708	2
ACATGGG	23165	0.0	22.456463	3
GCAGAGT	30520	0.0	21.825607	9
GAGTACT	16955	0.0	20.99799	12-13
CATGGGG	18160	0.0	20.160637	4
CAGAGTA	29505	0.0	19.623455	10-11
AGAGTAC	26895	0.0	19.572899	10-11
GTGGTAT	4235	0.0	19.155535	1
GTACTTT	19410	0.0	18.264997	14-15
AGTACTT	18820	0.0	17.18394	12-13
>>END_MODULE