FastQCFastQC Report
Fri 27 May 2016
SRR2139335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18259709
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246400.047.807971
GTATCAA430200.034.0958671
TCAACGC536250.026.7191094
ATCAACG539300.026.5909523
CAACGCA546400.026.2152165
AACGCAG555700.025.8210436
TATCAAC563000.025.6717322
ACGCAGA642800.022.2902897
CGCAGAG648700.022.0875598
GTACATG544150.019.8714181
GTGGTAT116600.018.9582841
TACATGG561950.018.8228822
GCAGAGT756000.018.7836289
ACATGGG554450.018.7712083
GAGTACT444600.018.36497712-13
AGAGTAC689650.017.9069710-11
TGGTATC116850.017.2557932
CAGAGTA749200.017.21073510-11
CATGGGG397000.016.912454
GTACTTT506500.016.04408614-15
Produced by FastQC (version 0.11.5)