##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18259709
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622476185135262	31.0	31.0	34.0	30.0	34.0
2	31.7155823786677	31.0	31.0	34.0	30.0	34.0
3	31.79780000875151	31.0	31.0	34.0	30.0	34.0
4	35.56907489599095	37.0	35.0	37.0	33.0	37.0
5	35.42172325966421	37.0	35.0	37.0	33.0	37.0
6	35.451417161138764	37.0	35.0	37.0	33.0	37.0
7	35.357672348447615	37.0	35.0	37.0	32.0	37.0
8	35.33152412231761	37.0	35.0	37.0	32.0	37.0
9	36.93217334405494	39.0	37.0	39.0	33.0	39.0
10-11	36.827012659402186	39.0	37.0	39.0	32.0	39.0
12-13	36.78635508375298	39.0	37.0	39.0	32.0	39.0
14-15	37.90795324284741	40.0	37.0	41.0	32.5	41.0
16-17	37.87037838335759	40.0	37.0	41.0	32.0	41.0
18-19	37.896824122443576	40.0	37.0	41.0	32.0	41.0
20-21	37.8626010058053	40.0	37.0	41.0	32.0	41.0
22-23	37.77975283532843	40.0	37.0	41.0	32.0	41.0
24-25	37.678436116259	39.5	36.5	41.0	32.0	41.0
26-27	37.461769856575444	39.0	36.0	41.0	32.0	41.0
28-29	37.31594627855486	39.0	36.0	41.0	31.0	41.0
30-31	37.138697106239306	39.0	36.0	40.5	31.0	41.0
32-33	36.94889337930137	39.0	35.5	40.0	30.5	41.0
34-35	36.80820551682878	39.0	35.0	40.0	30.0	41.0
36-37	36.63816448662763	38.0	35.0	40.0	30.0	41.0
38-39	36.49034513411806	38.0	35.0	40.0	30.0	41.0
40-41	36.34500248271269	38.0	35.0	40.0	30.0	41.0
42-43	36.15261558959757	38.0	35.0	40.0	30.0	41.0
44-45	36.23747442752443	38.0	34.5	40.0	29.5	41.0
46-47	36.69634527133175	39.0	35.0	40.0	30.0	41.0
48-49	36.79603615496709	39.0	35.0	40.0	30.0	41.0
50-51	36.76238261261052	39.0	35.0	40.0	30.0	41.0
52-53	36.65204048315796	39.0	35.0	40.0	30.0	41.0
54-55	36.501911762999704	38.5	35.0	40.0	30.0	41.0
56-57	36.34127922803463	38.0	34.5	40.0	29.0	41.0
58-59	36.17848861597056	38.0	34.0	40.0	29.0	41.0
60-61	35.99129090443844	38.0	34.0	40.0	28.5	41.0
62-63	35.776328852985856	38.0	34.0	40.0	28.0	41.0
64-65	35.509344264079104	37.0	34.0	40.0	28.0	41.0
66-67	35.24704369728357	37.0	33.5	39.5	28.0	41.0
68-69	34.96863125610784	36.0	33.0	39.0	28.0	41.0
70-71	34.66270076976814	36.0	33.0	39.0	28.0	40.5
72-73	34.3599793402982	35.0	32.5	38.5	27.5	40.0
74-75	34.0457018891759	35.0	32.0	38.0	27.0	40.0
76-77	32.21405151697766	33.5	30.0	36.0	25.5	38.5
78-79	33.33993826991008	34.5	31.5	36.5	26.5	39.0
80-81	33.40142298773165	35.0	32.0	36.0	27.0	39.0
82-83	33.188999091518674	35.0	32.0	36.0	27.0	37.5
84-85	32.956672408821206	34.5	32.0	35.5	26.5	37.0
86-87	32.74610463126288	34.0	32.0	35.0	26.0	37.0
88-89	32.574299229365245	34.0	31.0	35.0	26.0	36.0
90-91	32.43876676825924	34.0	31.0	35.0	26.0	36.0
92-93	32.36124016241036	34.0	31.0	35.0	26.0	36.0
94-95	32.367825435721414	34.0	31.0	35.0	27.0	35.5
96-97	32.45437624176412	34.0	31.0	35.0	27.0	35.0
98-99	32.60134223167067	34.0	31.5	35.0	28.0	35.0
100	31.023614873158962	33.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	7.0
19	40.0
20	266.0
21	1436.0
22	6679.0
23	21972.0
24	55309.0
25	111563.0
26	190773.0
27	288802.0
28	396820.0
29	508546.0
30	625177.0
31	748297.0
32	896586.0
33	1087105.0
34	1352884.0
35	1719583.0
36	2307822.0
37	3130312.0
38	3192019.0
39	1556462.0
40	61248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.09325121227288	21.79784464254058	14.987434903809255	23.121469241377284
2	14.344835588304353	22.434118468806517	41.52667619256618	21.69436975032295
3	17.4347859231922	27.938458818728094	31.652012178946126	22.974743079133578
4	12.32478020323325	19.047674856154607	39.881303694379795	28.746241246232344
5	12.198929347669232	38.974761317390104	36.51203313262002	12.31427620232064
6	28.445946208671778	39.88019195705693	19.085194621666755	12.588667212604538
7	24.877091374377777	33.72965047679034	24.606358602875414	16.786899545956473
8	23.701259423137575	37.7499444268252	21.675630208564662	16.87316594147256
9	25.32408375182759	16.669438707922453	21.896844029661153	36.10963351058881
10-11	23.473074198073252	27.320337887648517	29.952191382991998	19.25439653128623
12-13	24.31517321361645	25.084347932356042	29.928289094270255	20.672189759757252
14-15	21.641200841049425	26.45686489095115	27.389066521778254	24.512867746221172
16-17	20.968094510613515	29.515064721756783	28.09460590552504	21.42223486210466
18-19	20.992269369806387	28.68391713193798	29.853299712492245	20.470513785763387
20-21	21.809172238335314	27.85200370853069	29.69606555739361	20.64275849574038
22-23	21.59113209890796	27.658811170222602	29.599101399461876	21.150955331407566
24-25	21.345803315684403	27.90735286639505	29.55777011061926	21.189073707301294
26-27	21.320767261134254	28.019010863213328	29.47316013037446	21.187061745277955
28-29	21.314617583843425	28.0211231425276	29.483547737031156	21.180711536597823
30-31	21.628020441160785	27.918863057375866	29.26927937204884	21.183837129414503
32-33	21.41309117087083	28.133468823484662	29.22663952453871	21.226800481105794
34-35	21.516066246604247	28.131754768916117	29.258713137656063	21.09346584682357
36-37	21.544065568373085	28.076690651965723	29.175022223959303	21.204221555701892
38-39	21.44129617255176	28.21310202019351	29.18500974815955	21.16059205909518
40-41	21.612733381709038	28.052718475725825	29.094357136309988	21.240191006255145
42-43	21.523171139124596	28.187353575871338	28.780316704887998	21.509158580116072
44-45	21.624746662655074	28.333649470392075	28.554583126205337	21.487020740747514
46-47	21.742067526819362	28.28386342157264	28.292197745909988	21.681871305698014
48-49	21.626391928936854	28.422203272724634	28.30230345753662	21.649101340801895
50-51	21.649181054424478	28.47778804597522	28.165393075971323	21.707637823628975
52-53	21.795900451281035	28.36764830892806	28.069661826377118	21.766789413413786
54-55	21.767237791949665	28.267723503366327	28.158953683970832	21.806085020713176
56-57	21.74626599330152	28.386778519046235	28.084125075634308	21.782830412017937
58-59	21.762568037884268	28.406513974189203	28.09819595553848	21.73272203238805
60-61	21.891708905194378	28.40613026196811	28.059401322976242	21.64275950986127
62-63	21.852162677905493	28.452508649689985	28.038273686132282	21.657054986272247
64-65	21.792627050491685	28.35566547034426	28.01279769300923	21.838909786154826
66-67	21.85742417214006	28.376559752537524	27.893064632555365	21.87295144276705
68-69	21.756689334141385	28.40656154570469	27.96523401522914	21.871515104924786
70-71	21.818280151109608	28.116868316003192	28.062715399615513	22.002136133271687
72-73	21.864548689380754	28.247566556992105	27.92538980206485	21.96249495156229
74-75	21.677234005741997	28.370202733490018	27.98488595135525	21.96767730941274
76-77	21.80585314504358	28.21350426108754	28.08143696420476	21.899205629664117
78-79	21.795855900743895	28.249912446975927	27.937796182456502	22.016435469823673
80-81	21.85229491247122	28.28694849716637	27.92020191211914	21.940554678243267
82-83	21.874959842554944	28.18088864340033	28.05770958331205	21.886441930732676
84-85	21.907401884243583	28.126853378537703	28.02212904319959	21.94361569401913
86-87	21.732324762697274	28.226793961248838	28.147978885743875	21.892902390310013
88-89	21.813797394590022	28.099973033134496	28.106610913551823	21.97961865872367
90-91	21.87837281394365	28.080880470033037	28.194088497500026	21.84665821852329
92-93	21.919374403519022	28.027359524010027	28.15837760283106	21.89488846963989
94-95	21.684509501357592	27.99123070059136	28.294124638199442	22.03013515985161
96-97	21.72349850872733	28.176307969738566	28.38213303971387	21.71806048182023
98-99	22.703120369493508	30.127403269676456	28.670041974621114	18.499434386208918
100	22.185839415169795	30.61695893974163	22.529019338109528	24.66818230697905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4292.0
1	3850.0
2	5500.0
3	8045.5
4	10574.0
5	17016.0
6	23025.0
7	27905.0
8	31429.5
9	31185.5
10	28157.5
11	24723.0
12	26106.5
13	33561.0
14	50732.5
15	77987.5
16	101228.0
17	113688.5
18	118872.0
19	116840.0
20	111085.5
21	105468.5
22	101301.0
23	105392.0
24	121588.5
25	149962.5
26	189481.0
27	234490.5
28	288222.5
29	352750.5
30	415173.5
31	473503.5
32	535182.5
33	590584.5
34	637666.5
35	680555.0
36	722449.5
37	753298.0
38	759088.0
39	747686.5
40	736097.5
41	722147.0
42	702441.5
43	691402.5
44	684677.0
45	680569.0
46	673279.0
47	663687.0
48	657017.5
49	644122.5
50	630948.5
51	617247.5
52	597257.0
53	572727.5
54	546573.0
55	516770.5
56	483356.5
57	443751.5
58	397554.5
59	346837.5
60	290756.0
61	237710.5
62	191202.5
63	149264.0
64	112425.0
65	84333.5
66	63968.5
67	49294.0
68	38121.0
69	27868.5
70	19855.5
71	14421.0
72	10196.0
73	6961.0
74	4831.5
75	3358.5
76	2371.5
77	1662.5
78	1167.5
79	824.5
80	585.0
81	423.0
82	315.5
83	209.0
84	137.0
85	106.5
86	87.5
87	72.0
88	58.0
89	42.5
90	30.0
91	21.5
92	15.0
93	8.0
94	3.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.12656828211227242
3	5.476538536293212E-6
4	0.0
5	0.0
6	0.0
7	3.285923121775928E-5
8	0.0
9	0.0
10-11	1.6429615608879638E-4
12-13	4.0252558241755113E-4
14-15	7.667153950810498E-4
16-17	2.491825034013412E-4
18-19	0.0011035225150630823
20-21	6.572297241343956E-5
22-23	0.0018898520286945272
24-25	1.369916384139678E-4
26-27	0.005430831926690294
28-29	0.002721838927059875
30-31	0.004713028244587423
32-33	0.017173100443458395
34-35	0.0038607560261879835
36-37	0.0022154667935718663
38-39	0.0011280099882905285
40-41	6.404036111315802E-4
42-43	0.001203500736112832
44-45	4.0253906691961835E-5
46-47	2.3562955694108966E-4
48-49	1.352273829335811E-4
50-51	5.552575376507665E-4
52-53	2.997428865467779E-5
54-55	0.0
56-57	0.0
58-59	1.6388683162814238E-4
60-61	0.0
62-63	1.2651871083633074E-5
64-65	4.478250114811137E-5
66-67	0.0
68-69	2.19733512443476E-4
70-71	9.972290660760323E-5
72-73	9.906397349236761E-4
74-75	1.1953087750656677E-4
76-77	0.0020322959172421407
78-79	0.0026989961243608965
80-81	0.0027898856916490065
82-83	8.687574932596183E-5
84-85	0.0
86-87	0.001709357591997663
88-89	4.382048811298766E-4
90-91	5.604904447082988E-4
92-93	7.572141787478197E-5
94-95	2.457257641497905E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	774.0
20-21	2517.0
22-23	4887.0
24-25	9964.0
26-27	13711.0
28-29	22550.0
30-31	46944.0
32-33	60058.0
34-35	84358.0
36-37	115571.0
38-39	140075.0
40-41	144860.0
42-43	169016.0
44-45	209171.0
46-47	183454.0
48-49	171978.0
50-51	160395.0
52-53	156715.0
54-55	169230.0
56-57	178700.0
58-59	181912.0
60-61	181140.0
62-63	175723.0
64-65	183701.0
66-67	196151.0
68-69	202851.0
70-71	204773.0
72-73	198297.0
74-75	202514.0
76-77	190028.0
78-79	210273.0
80-81	219981.0
82-83	218092.0
84-85	223110.0
86-87	239450.0
88-89	267760.0
90-91	294615.0
92-93	324993.0
94-95	389191.0
96-97	772913.0
98-99	1280093.0
100-101	9857220.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.40883249157865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.81724664953421	73.1932560150469
2	7.418246579867435	12.226580795630435
3	1.9374390426234482	4.789862685786008
4	0.7689976524712406	2.5348879491567873
5	0.38406818966618944	1.5825305553772433
6	0.2035388679698864	1.0064040285633546
7	0.1298767765409692	0.7492095465757647
8	0.08421306791019724	0.5551920485610706
9	0.05428157806940724	0.4025953327050298
>10	0.19666390758325122	2.5336438648582806
>50	0.004257952454013921	0.23871354488910324
>100	0.0011031038317935755	0.139083698867434
>500	3.9990691385487674E-5	0.024526813920990768
>1k	2.6640786537721224E-5	0.02351312006159565
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.190615414517285E-5	0.0	0.0	0.0
3	0.0	3.285923121775927E-5	0.0	0.0	0.0
4	0.0	7.119500097181177E-5	0.0	1.0953077072586425E-5	0.0
5	0.0	8.214807804439819E-5	0.0	1.0953077072586425E-5	0.0
6	5.476538536293212E-6	8.214807804439819E-5	0.0	1.0953077072586425E-5	0.0
7	1.0953077072586425E-5	8.214807804439819E-5	0.0	2.7382692681466063E-5	0.0
8	2.190615414517285E-5	9.310115511698461E-5	0.0	2.7382692681466063E-5	0.0
9	2.190615414517285E-5	1.8072577169767602E-4	0.0	4.38123082903457E-5	0.0
10-11	2.190615414517285E-5	1.8072577169767602E-4	0.0	4.38123082903457E-5	0.0
12-13	2.190615414517285E-5	1.8072577169767602E-4	0.0	4.38123082903457E-5	0.0
14-15	3.285923121775927E-5	2.984713502279801E-4	0.0	4.9288846826638915E-5	8.214807804439818E-6
16-17	4.9288846826638915E-5	3.1216269656871313E-4	0.0	6.0241923899225335E-5	1.0953077072586425E-5
18-19	4.9288846826638915E-5	3.395453892501792E-4	0.0	6.0241923899225335E-5	1.0953077072586425E-5
20-21	4.9288846826638915E-5	3.6966635119979184E-4	0.0	6.0241923899225335E-5	1.0953077072586425E-5
22-23	5.750365463107873E-5	4.1895519802643075E-4	0.0	7.119500097181177E-5	1.0953077072586425E-5
24-25	6.571846243551854E-5	4.627675063167765E-4	0.0	1.0131596292142443E-4	1.0953077072586425E-5
26-27	6.571846243551854E-5	4.8467366046194926E-4	0.0	1.6429615608879638E-4	1.0953077072586425E-5
28-29	6.571846243551854E-5	4.983650068026823E-4	0.0	4.4907615997604345E-4	1.0953077072586425E-5
30-31	7.119500097181177E-5	5.55868661433761E-4	0.0	0.0012678186711518786	1.0953077072586425E-5
32-33	7.667153950810498E-5	5.586069307019077E-4	0.0	0.0030285258105701464	1.6429615608879636E-5
34-35	7.667153950810498E-5	6.078957775285465E-4	0.0	0.005213664686551139	1.6429615608879636E-5
36-37	7.667153950810498E-5	6.489698165507457E-4	0.0	0.008611856848321076	2.190615414517285E-5
38-39	7.667153950810498E-5	6.681377014277718E-4	0.0	0.013830998073408509	2.190615414517285E-5
40-41	9.857769365327783E-5	7.420709716677303E-4	0.0	0.01924455641653435	2.190615414517285E-5
42-43	1.0953077072586425E-4	7.776684721536362E-4	0.0	0.025049687265005154	2.190615414517285E-5
44-45	1.314369248710371E-4	8.707696272706208E-4	0.0	0.031065664847123246	2.190615414517285E-5
46-47	1.67034425356943E-4	9.310115511698461E-4	0.0	0.03842065610136503	2.190615414517285E-5
48-49	1.8072577169767602E-4	9.447028975105792E-4	0.0	0.045518250044401035	2.190615414517285E-5
50-51	2.0537019511099547E-4	0.0010487571297001503	0.0	0.0526541797571911	2.190615414517285E-5
52-53	2.0810846437914208E-4	0.0010624484760408833	0.0	0.059464255427071705	2.190615414517285E-5
54-55	2.1632327218358192E-4	0.001070663283845323	0.0	0.0664030297525552	3.0120961949612667E-5
56-57	2.2453807998802172E-4	0.0011172138614038154	0.0	0.07358551004290376	3.833576975405249E-5
58-59	2.3001461852431492E-4	0.0011226903999401086	0.0	0.0803736795586392	4.1074039022199094E-5
60-61	2.3549115706060814E-4	0.0011391200155489883	0.0	0.08712351330462056	4.38123082903457E-5
62-63	2.3549115706060814E-4	0.0011637644389623078	0.0	0.09341605608282147	4.38123082903457E-5
64-65	2.4644423413319456E-4	0.0012431742477385592	0.0	0.09931428808640927	4.6550577558492304E-5
66-67	2.519207726694878E-4	0.0012541273248111457	0.0	0.10546717913193468	5.202711609478552E-5
68-69	2.519207726694878E-4	0.0012760334789563184	0.0	0.11170221825550451	5.4765385362932125E-5
70-71	2.57397311205781E-4	0.0012924630945651981	0.0	0.11709934698302146	5.4765385362932125E-5
72-73	2.628738497420742E-4	0.0012952013638333447	0.0	0.12146414819644716	8.76246165806914E-5
74-75	2.6561211901022076E-4	0.0013390136721236905	0.0	0.12514985863137248	8.76246165806914E-5
76-77	2.683503882783674E-4	0.001377349441877743	0.0	0.12848780886924321	8.76246165806914E-5
78-79	2.738269268146606E-4	0.0014074704038273555	0.0	0.13069759216863752	8.76246165806914E-5
80-81	2.7930346535095385E-4	0.001412946942363649	0.0	0.1314478779481097	9.583942438513122E-5
82-83	2.7930346535095385E-4	0.0014156852116317955	0.0	0.13195719603198497	1.0405423218957104E-4
84-85	2.7930346535095385E-4	0.0014239000194362353	0.0	0.13216256622709596	1.0405423218957104E-4
86-87	2.7930346535095385E-4	0.0014512827121177013	0.0	0.13226114392074922	1.0405423218957104E-4
88	2.8478000388724707E-4	0.0014896184818717537	0.0	0.1322638821900174	1.0405423218957104E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24640	0.0	47.80797	1
GTATCAA	43020	0.0	34.095867	1
TCAACGC	53625	0.0	26.719109	4
ATCAACG	53930	0.0	26.590952	3
CAACGCA	54640	0.0	26.215216	5
AACGCAG	55570	0.0	25.821043	6
TATCAAC	56300	0.0	25.671732	2
ACGCAGA	64280	0.0	22.290289	7
CGCAGAG	64870	0.0	22.087559	8
GTACATG	54415	0.0	19.871418	1
GTGGTAT	11660	0.0	18.958284	1
TACATGG	56195	0.0	18.822882	2
GCAGAGT	75600	0.0	18.783628	9
ACATGGG	55445	0.0	18.771208	3
GAGTACT	44460	0.0	18.364977	12-13
AGAGTAC	68965	0.0	17.90697	10-11
TGGTATC	11685	0.0	17.255793	2
CAGAGTA	74920	0.0	17.210735	10-11
CATGGGG	39700	0.0	16.91245	4
GTACTTT	50650	0.0	16.044086	14-15
>>END_MODULE