FastQCFastQC Report
Fri 27 May 2016
SRR2139334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21445624
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246850.047.3179471
GTATCAA434000.034.005111
TCAACGC521900.027.5576274
ATCAACG525900.027.347963
CAACGCA535650.026.8268345
AACGCAG548250.026.2256136
TATCAAC563900.025.7839642
ACGCAGA634800.022.6170927
CGCAGAG641800.022.3965858
GTACATG576800.020.0754591
TACATGG578350.019.6581552
ACATGGG566000.019.4454563
GCAGAGT757050.018.7931469
GAGTACT442500.018.00768312-13
AGAGTAC683350.017.88873710-11
CAGAGTA749250.017.20929110-11
CATGGGG406600.016.9476594
GTGGTAT132900.016.1034741
GTACTTT515550.015.42041114-15
AGTACTT507250.015.34888812-13
Produced by FastQC (version 0.11.5)