##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21445624
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.615612024159333	31.0	31.0	34.0	30.0	34.0
2	31.71340857230361	31.0	31.0	34.0	30.0	34.0
3	31.800901946243204	31.0	31.0	34.0	30.0	34.0
4	35.56945239737487	37.0	35.0	37.0	33.0	37.0
5	35.42440509075418	37.0	35.0	37.0	33.0	37.0
6	35.45390621415353	37.0	35.0	37.0	33.0	37.0
7	35.36105501056999	37.0	35.0	37.0	32.0	37.0
8	35.3337145610685	37.0	35.0	37.0	32.0	37.0
9	36.93918596166752	39.0	37.0	39.0	33.0	39.0
10-11	36.83268460735859	39.0	37.0	39.0	32.5	39.0
12-13	36.793954911267676	39.0	37.0	39.0	32.0	39.0
14-15	37.92241780887327	40.0	37.0	41.0	33.0	41.0
16-17	37.88315089362753	40.0	37.0	41.0	32.0	41.0
18-19	37.89931290877803	40.0	37.0	41.0	32.0	41.0
20-21	37.86231181241696	40.0	37.0	41.0	32.0	41.0
22-23	37.78120108761172	40.0	37.0	41.0	32.0	41.0
24-25	37.68202834198351	39.5	37.0	41.0	32.0	41.0
26-27	37.46717690049868	39.0	36.0	41.0	32.0	41.0
28-29	37.32325293459879	39.0	36.0	41.0	31.0	41.0
30-31	37.14889494096518	39.0	36.0	40.5	31.0	41.0
32-33	36.96624637437459	39.0	36.0	40.0	30.5	41.0
34-35	36.82342816684117	39.0	35.0	40.0	30.0	41.0
36-37	36.65272187276337	38.0	35.0	40.0	30.0	41.0
38-39	36.503496715412325	38.0	35.0	40.0	30.0	41.0
40-41	36.35690594098897	38.0	35.0	40.0	30.0	41.0
42-43	36.16336325707957	38.0	35.0	40.0	30.0	41.0
44-45	36.24998511819044	38.0	34.5	40.0	29.5	41.0
46-47	36.71529818955794	39.0	35.0	40.0	30.0	41.0
48-49	36.81657007750107	39.0	35.0	40.0	30.0	41.0
50-51	36.783243382606585	39.0	35.0	40.0	30.0	41.0
52-53	36.67234839268255	39.0	35.0	40.0	30.0	41.0
54-55	36.528319010077	38.5	35.0	40.0	30.0	41.0
56-57	36.370092005439105	38.0	35.0	40.0	29.5	41.0
58-59	36.20923749810138	38.0	34.0	40.0	29.0	41.0
60-61	36.027132187524806	38.0	34.0	40.0	28.5	41.0
62-63	35.82027218038007	38.0	34.0	40.0	28.0	41.0
64-65	35.555548733184125	37.0	34.0	40.0	28.0	41.0
66-67	35.29263456962708	37.0	33.5	40.0	28.0	41.0
68-69	35.01635989485541	36.0	33.0	39.0	28.0	41.0
70-71	34.711474574843294	36.0	33.0	39.0	28.0	40.5
72-73	34.40561926413866	35.5	32.5	39.0	27.5	40.0
74-75	34.08660077541811	35.0	32.0	38.0	27.0	40.0
76-77	32.25155963573373	33.5	30.0	36.0	25.5	38.5
78-79	33.37653124386249	35.0	31.5	37.0	26.5	39.0
80-81	33.43065560056414	35.0	32.0	36.0	27.0	39.0
82-83	33.21222928786679	35.0	32.0	36.0	27.0	38.0
84-85	32.973520899323525	34.5	32.0	36.0	26.5	37.0
86-87	32.75150749105068	34.0	31.5	35.0	26.0	37.0
88-89	32.57717809379924	34.0	31.0	35.0	26.0	36.0
90-91	32.43528350604533	34.0	31.0	35.0	26.0	36.0
92-93	32.35468088099991	34.0	31.0	35.0	26.0	36.0
94-95	32.357402860169195	34.0	31.0	35.0	26.5	35.5
96-97	32.44616502173434	34.0	31.0	35.0	27.0	35.0
98-99	32.59314420855489	34.0	31.5	35.0	28.0	35.0
100	31.013583807417863	33.0	29.0	34.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	6.0
19	39.0
20	254.0
21	1705.0
22	7780.0
23	25590.0
24	64297.0
25	130130.0
26	224585.0
27	340554.0
28	467820.0
29	601136.0
30	737741.0
31	883870.0
32	1059099.0
33	1281666.0
34	1589085.0
35	2022490.0
36	2712738.0
37	3699376.0
38	3810385.0
39	1722452.0
40	62823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.9137651578709	21.806961644016514	14.955825020526332	23.323448177586254
2	14.247345689658472	22.39670383038858	41.784218153248226	21.57173232670472
3	17.59258205776619	27.735980076867893	31.49605719096819	23.175380674397722
4	12.293305151671035	19.06685018817825	40.034078747253986	28.60576591289673
5	12.181184375889458	38.925008663772154	36.675407533023986	12.218399427314402
6	28.436626511776947	40.03007793105018	19.103846080673613	12.429449476499261
7	24.892482702411154	33.59743078528067	24.63639310201305	16.87369341029513
8	23.375048448112302	38.07912047697936	21.759693259566614	16.78613781534172
9	25.022699269557275	16.882754262594553	21.908152451054814	36.18639401679336
10-11	23.32723798011165	27.39871026519391	30.120195211398304	19.15385654329613
12-13	24.123095211988122	25.0538206575874	30.112546300150804	20.71053783027367
14-15	21.3845086055725	26.567061321743736	27.438351624987984	24.610078447695784
16-17	20.897446592367274	29.65255026712501	28.12452190025011	21.325481240257606
18-19	20.95886914345486	28.877314901166724	29.72061198369367	20.443203971684753
20-21	21.7159630425006	28.092083922689415	29.54086930667103	20.651083728138957
22-23	21.476193945348083	27.928289727199353	29.417552779912615	21.177963547539957
24-25	21.272843403616314	28.13421967993931	29.405048553418766	21.187888363025607
26-27	21.275844758758677	28.239610180162344	29.296212907363493	21.188332153715482
28-29	21.266027448224044	28.27620162008297	29.33118906832196	21.126581863371026
30-31	21.564065845359927	28.143512833319285	29.150256419419307	21.142164901901484
32-33	21.37506386389722	28.339193173812255	29.116081940148923	21.1696610221416
34-35	21.48325682485678	28.315570872971268	29.117183635714206	21.08398866645775
36-37	21.501755807595046	28.257688925331365	29.08033914531696	21.16021612175663
38-39	21.34510090346432	28.377936657521435	29.13833662764481	21.138625811369433
40-41	21.522102825745776	28.25858654124608	29.014379481767826	21.204931151240324
42-43	21.447833462283974	28.36045100397364	28.767359600090703	21.424355933651682
44-45	21.53094203749838	28.46826105228482	28.58609476449365	21.414702145723147
46-47	21.64575201615826	28.39555966144789	28.37476997559632	21.58391834679753
48-49	21.555124222664578	28.488143976093166	28.417657034371697	21.539074766870563
50-51	21.559324938883066	28.585451628302366	28.308007427508507	21.54721600530606
52-53	21.697542509311496	28.496354691544784	28.188591932777328	21.617510866366395
54-55	21.665005819592995	28.408532272007236	28.284931414149106	21.641530494250667
56-57	21.673451463676997	28.485882926883697	28.253903325068986	21.586762284370316
58-59	21.65030668393716	28.507411670996248	28.241356698028774	21.600924947037818
60-61	21.740067680231512	28.49876026566741	28.220539050131784	21.540633003969294
62-63	21.678988403687075	28.5540933372825	28.174116364060165	21.59280189497026
64-65	21.67630058568927	28.489885411315342	28.159444165014254	21.674369837981132
66-67	21.732409865693462	28.476766144978587	28.077399460700782	21.71342452862717
68-69	21.662624578469007	28.51810690584628	28.124554758927513	21.694713756757203
70-71	21.694447586355384	28.28668358601699	28.19198307019593	21.826885757431693
72-73	21.726466103777906	28.360210875682284	28.109341663500814	21.803981357038992
74-75	21.556967736525635	28.469680197811243	28.145200921434594	21.82815114422852
76-77	21.664253178344577	28.354965278294546	28.16992342696427	21.810858116396602
78-79	21.653155106717783	28.41032798091908	28.05534891807568	21.88116799428746
80-81	21.703658451510094	28.403501927478143	28.084936053165737	21.807903567846022
82-83	21.686152646910124	28.317458660173926	28.227441454928144	21.768947237987806
84-85	21.78541743258459	28.266518690997096	28.164513404066838	21.78355047235148
86-87	21.608761282387363	28.305390383095418	28.285175654698165	21.80067267981905
88-89	21.695632252539234	28.271371660047567	28.210129924995076	21.822866162418126
90-91	21.75916723102408	28.268665922668728	28.2805444538769	21.691622392430297
92-93	21.74824660659246	28.145729944556397	28.30832916362207	21.79769428522907
94-95	21.547776925737143	28.181374391668047	28.428182162946925	21.842666519647892
96-97	21.547685738009623	28.309669097800565	28.544286114601313	21.5983590495885
98-99	22.532810371102045	30.299122353560648	28.824580249784287	18.34348702555302
100	22.05242923016203	30.882458891741116	22.648418449373008	24.41669342872385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4169.0
1	3709.5
2	5200.0
3	7582.5
4	10081.5
5	16536.0
6	22605.5
7	27361.0
8	30795.5
9	30509.5
10	27353.0
11	23889.0
12	24963.5
13	32137.5
14	48606.5
15	73955.5
16	95819.5
17	108525.0
18	114138.5
19	112873.5
20	108475.5
21	104207.5
22	102080.0
23	108527.5
24	127489.0
25	160429.0
26	207597.5
27	262293.5
28	325580.5
29	399294.0
30	470885.0
31	543603.0
32	621160.0
33	689575.0
34	749272.0
35	801264.0
36	850649.0
37	888332.5
38	896362.0
39	886356.5
40	880467.0
41	871984.0
42	854835.5
43	843516.0
44	831529.0
45	821391.0
46	808937.0
47	793112.0
48	779521.0
49	759692.0
50	738242.5
51	714616.0
52	683432.5
53	645587.0
54	604496.0
55	561038.5
56	516945.5
57	469340.0
58	418079.5
59	363789.5
60	305386.0
61	248875.5
62	198616.0
63	154419.5
64	116292.0
65	86909.5
66	65509.5
67	50211.0
68	38521.5
69	28409.0
70	20467.5
71	14793.0
72	10411.0
73	7199.5
74	4952.0
75	3409.0
76	2422.0
77	1710.0
78	1176.5
79	836.5
80	620.5
81	465.5
82	353.0
83	251.5
84	189.0
85	139.0
86	100.0
87	79.0
88	59.5
89	41.0
90	27.0
91	19.5
92	15.0
93	9.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.1262075657019819
3	0.0
4	0.0
5	0.0
6	0.0
7	2.3314779742478E-5
8	0.0
9	0.0
10-11	2.44805187296019E-4
12-13	4.406493371328342E-4
14-15	8.789671962914205E-4
16-17	2.1915892957929318E-4
18-19	0.0010071984848750496
20-21	5.36272554603563E-5
22-23	0.0019005603061660401
24-25	1.5629146389209973E-4
26-27	0.005288802580319488
28-29	0.0026766781592545024
30-31	0.004808129892845119
32-33	0.017377382470774593
34-35	0.003929423603850177
36-37	0.0021777240348936984
38-39	0.001219148805010028
40-41	6.801245415423649E-4
42-43	0.001311630256715589
44-45	3.148884363592182E-5
46-47	2.3957953107782895E-4
48-49	1.1833686120991897E-4
50-51	5.269048424021403E-4
52-53	2.2543414106887044E-5
54-55	0.0
56-57	0.0
58-59	1.5430161661032215E-4
60-61	0.0
62-63	1.3112600020618251E-5
64-65	3.707881740925694E-5
66-67	2.677007410893466E-6
68-69	2.0582095791944475E-4
70-71	8.772366038157709E-5
72-73	9.297636293842276E-4
74-75	1.2926324949712382E-4
76-77	0.0020403275724575243
78-79	0.002576317869441605
80-81	0.0026770016073991857
82-83	8.601873950867995E-5
84-85	0.0
86-87	0.0016248632100797577
88-89	4.3610404458154585E-4
90-91	5.277906245689843E-4
92-93	6.832031988484707E-5
94-95	2.6730679515926105E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	798.0
20-21	2676.0
22-23	5243.0
24-25	10993.0
26-27	14118.0
28-29	21410.0
30-31	42752.0
32-33	55031.0
34-35	76762.0
36-37	104225.0
38-39	127319.0
40-41	133634.0
42-43	157150.0
44-45	196172.0
46-47	174520.0
48-49	165574.0
50-51	157183.0
52-53	156684.0
54-55	171691.0
56-57	183156.0
58-59	186406.0
60-61	190341.0
62-63	185108.0
64-65	196619.0
66-67	212540.0
68-69	222001.0
70-71	225195.0
72-73	221247.0
74-75	226773.0
76-77	213745.0
78-79	237299.0
80-81	252039.0
82-83	250922.0
84-85	259528.0
86-87	281524.0
88-89	314626.0
90-91	349983.0
92-93	388937.0
94-95	471006.0
96-97	945810.0
98-99	1581330.0
100-101	1.2075554E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.08900525636739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30633372114225	72.48979093005379
2	7.675272112012271	12.601109054940506
3	2.122864079106993	5.227914016451023
4	0.8325332977735032	2.733673210281199
5	0.4065578224661427	1.6686963612720236
6	0.20795699833470482	1.0242589877637582
7	0.1314010759342321	0.7550608529140256
8	0.08126809586000822	0.5336973718581747
9	0.057074055421025956	0.42166371919129225
>10	0.17422119039950468	2.1790525159691234
>50	0.0036066739173979774	0.19704589681336812
>100	8.448005649526612E-4	0.11282145699956742
>500	4.4122732386969575E-5	0.02446128423401401
>1k	2.195433463449226E-5	0.030754341258080256
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6629559484956E-6
2	0.0	1.39888678454868E-5	0.0	4.6629559484956E-6	4.6629559484956E-6
3	0.0	1.39888678454868E-5	0.0	4.6629559484956E-6	4.6629559484956E-6
4	0.0	4.6629559484956E-5	0.0	4.6629559484956E-6	4.6629559484956E-6
5	0.0	6.0618427330442795E-5	0.0	4.6629559484956E-6	4.6629559484956E-6
6	5.12925154334516E-5	6.0618427330442795E-5	0.0	4.6629559484956E-6	1.86518237939824E-5
7	5.12925154334516E-5	6.52813832789384E-5	0.0	4.6629559484956E-6	1.86518237939824E-5
8	5.12925154334516E-5	6.52813832789384E-5	0.0	4.6629559484956E-6	1.86518237939824E-5
9	5.12925154334516E-5	1.1657389871239E-4	0.0	9.3259118969912E-6	6.52813832789384E-5
10-11	5.12925154334516E-5	1.1657389871239E-4	0.0	9.3259118969912E-6	7.69387731501774E-5
12-13	5.12925154334516E-5	1.328942445321246E-4	0.0	9.3259118969912E-6	8.85961630214164E-5
14-15	5.12925154334516E-5	1.8651823793982398E-4	0.0	9.3259118969912E-6	9.09276409956642E-5
16-17	8.393320707292079E-5	1.98175627811063E-4	0.0	2.3314779742478E-5	1.025850308669032E-4
18-19	8.393320707292079E-5	2.3314779742478E-4	0.0	2.3314779742478E-5	1.025850308669032E-4
20-21	8.393320707292079E-5	2.611255331157536E-4	0.0	2.56462577167258E-5	1.04916508841151E-4
22-23	8.393320707292079E-5	2.7977735690973597E-4	0.0	3.4972169613716996E-5	1.1657389871238999E-4
24-25	8.393320707292079E-5	3.077550926007096E-4	0.0	5.828694935619499E-5	1.2123685466088559E-4
26-27	8.62646850471686E-5	3.3106987234318757E-4	0.0	9.092764099566419E-5	1.258998106093812E-4
28-29	9.09276409956642E-5	3.3340135031743537E-4	0.0	3.007606586779662E-4	1.258998106093812E-4
30-31	9.3259118969912E-5	3.8702534372513477E-4	0.0	8.906245861626597E-4	1.258998106093812E-4
32-33	9.3259118969912E-5	3.96351255622126E-4	0.0	0.0023734445777842603	1.352257225063724E-4
34-35	9.3259118969912E-5	4.6629559484956E-4	0.0	0.00415469375010958	1.3988867845486798E-4
36-37	9.3259118969912E-5	5.292455001542506E-4	0.0	0.006712325087859416	1.492145903518592E-4
38-39	9.79220749184076E-5	5.409028900254896E-4	0.0	0.011006907516423864	1.608719802230982E-4
40-41	9.79220749184076E-5	5.968583614074368E-4	0.0	0.015404074975855215	1.6786641414584158E-4
42-43	9.79220749184076E-5	6.038527953301802E-4	0.0	0.020015738408917363	1.6786641414584158E-4
44-45	1.002535528926554E-4	6.737971345576143E-4	0.0	0.025480722780554205	1.6786641414584158E-4
46-47	1.025850308669032E-4	7.367470398623048E-4	0.0	0.03123247894302353	1.701978921200894E-4
48-49	1.025850308669032E-4	7.390785178365526E-4	0.0	0.03773963396914914	1.771923260428328E-4
50-51	1.142424207381422E-4	7.880395552957564E-4	0.0	0.04434237959221891	1.86518237939824E-4
52-53	1.1657389871239E-4	7.903710332700042E-4	0.0	0.050467172230567875	1.958441498368152E-4
54-55	1.1890537668663779E-4	8.230117249094734E-4	0.0	0.056636262950427556	2.075015397080542E-4
56-57	1.2123685466088559E-4	8.50989460600447E-4	0.0	0.06302218112189228	2.09833017682302E-4
58-59	1.2123685466088559E-4	8.719727623686771E-4	0.0	0.06900708508178638	2.09833017682302E-4
60-61	1.258998106093812E-4	8.906245861626596E-4	0.0	0.07447906388734597	2.09833017682302E-4
62-63	1.445516344033636E-4	9.046134540081464E-4	0.0	0.079757530021043	2.09833017682302E-4
64-65	1.445516344033636E-4	9.768892712098281E-4	0.0	0.0850989460600447	2.09833017682302E-4
66-67	1.51546068326107E-4	9.955410950038106E-4	0.0	0.09042171027525242	2.144959736307976E-4
68-69	1.538775463003548E-4	0.0010118614408235452	0.0	0.09591234090460599	2.1915892957929318E-4
70-71	1.655349361715938E-4	0.0010235188306947842	0.0	0.10102527210213141	2.284848414762844E-4
72-73	1.958441498368152E-4	0.0010351762205660232	0.0	0.10518229733021525	2.4247370932177118E-4
74-75	1.958441498368152E-4	0.001060822478282749	0.0	0.1084137258025227	2.4247370932177118E-4
76-77	2.028385837595586E-4	0.0011027890818192092	0.0	0.11139568613158563	2.4247370932177118E-4
78-79	2.051700617338064E-4	0.0011191094276389439	0.0	0.11354764030181635	2.4247370932177118E-4
80-81	2.051700617338064E-4	0.0011214409056131917	0.0	0.1142330948262452	2.471366652702668E-4
82-83	2.051700617338064E-4	0.0011237723835874395	0.0	0.1147157107669145	2.634570110900014E-4
84-85	2.051700617338064E-4	0.0011261038615616873	0.0	0.11499548812382424	2.68119967038497E-4
86-87	2.051700617338064E-4	0.0011424242073814219	0.0	0.11511439350051086	2.751144009612404E-4
88	2.144959736307976E-4	0.0011704019430723955	0.0	0.11512838236835636	2.7977735690973597E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	24685	0.0	47.317947	1
GTATCAA	43400	0.0	34.00511	1
TCAACGC	52190	0.0	27.557627	4
ATCAACG	52590	0.0	27.34796	3
CAACGCA	53565	0.0	26.826834	5
AACGCAG	54825	0.0	26.225613	6
TATCAAC	56390	0.0	25.783964	2
ACGCAGA	63480	0.0	22.617092	7
CGCAGAG	64180	0.0	22.396585	8
GTACATG	57680	0.0	20.075459	1
TACATGG	57835	0.0	19.658155	2
ACATGGG	56600	0.0	19.445456	3
GCAGAGT	75705	0.0	18.793146	9
GAGTACT	44250	0.0	18.007683	12-13
AGAGTAC	68335	0.0	17.888737	10-11
CAGAGTA	74925	0.0	17.209291	10-11
CATGGGG	40660	0.0	16.947659	4
GTGGTAT	13290	0.0	16.103474	1
GTACTTT	51555	0.0	15.420411	14-15
AGTACTT	50725	0.0	15.348888	12-13
>>END_MODULE