FastQCFastQC Report
Fri 27 May 2016
SRR2139333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10313852
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA194000.052.604921
CTTATAC106000.039.442611
GTATCAA360550.036.4528241
TATACAC146700.031.6051463
TATCAAC459200.030.2071152
TTATACA147600.029.8259472
ATCAACG463500.029.7799053
TCAACGC464000.029.7561244
CAACGCA475800.029.06295
AACGCAG490950.028.2455626
GGACCGA33550.028.155526
ACCGAAC33300.027.344678
CCGAACC34650.025.788099
GTGGTAT85250.025.3705181
AGGACCG41150.025.3479965
GACCGAA36350.025.2843887
TGGTATC88900.024.9993552
ACGCAGA561350.024.6804897
CGCAGAG570250.024.317688
TACACAT212500.021.6377565
Produced by FastQC (version 0.11.5)