##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139333_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10313852
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.532951510260183	25.0	18.0	32.0	18.0	33.0
2	27.309954806409866	29.0	25.0	31.0	18.0	33.0
3	29.52671349171968	31.0	29.0	33.0	25.0	33.0
4	29.518698057718883	31.0	29.0	33.0	25.0	33.0
5	31.50454786436726	33.0	32.0	33.0	29.0	33.0
6	35.553442108729115	37.0	36.0	38.0	29.0	38.0
7	36.05717563137419	38.0	36.0	38.0	33.0	38.0
8	36.47866878446578	38.0	37.0	38.0	34.0	38.0
9	36.69666919789037	38.0	38.0	38.0	34.0	38.0
10-11	36.831385790682276	38.0	38.0	38.0	35.0	38.0
12-13	36.9030182418751	38.0	38.0	38.0	35.0	38.0
14-15	36.93187234992319	38.0	38.0	38.0	35.0	38.0
16-17	36.96512083943031	38.0	38.0	38.0	35.0	38.0
18-19	36.981608083963195	38.0	38.0	38.0	35.0	38.0
20-21	36.99246562069568	38.0	38.0	38.0	35.0	38.0
22-23	37.01287550697886	38.0	38.0	38.0	36.0	38.0
24-25	37.018070942453576	38.0	38.0	38.0	36.0	38.0
26-27	36.98849165804001	38.0	38.0	38.0	36.0	38.0
28-29	36.95884517668437	38.0	38.0	38.0	35.0	38.0
30-31	36.94989216800576	38.0	38.0	38.0	35.0	38.0
32-33	36.91261895891185	38.0	38.0	38.0	35.0	38.0
34-35	36.88380700538667	38.0	38.0	38.0	35.0	38.0
36-37	36.859507330980584	38.0	38.0	38.0	35.0	38.0
38-39	36.81774855767594	38.0	38.0	38.0	35.0	38.0
40-41	36.84738117896843	38.0	38.0	38.0	35.0	38.0
42-43	36.79909366721735	38.0	38.0	38.0	35.0	38.0
44-45	36.753605930279676	38.0	38.0	38.0	35.0	38.0
46-47	36.70311605175347	38.0	38.0	38.0	35.0	38.0
48-49	36.661209000729116	38.0	38.0	38.0	34.5	38.0
50-51	36.62980766292707	38.0	38.0	38.0	34.0	38.0
52-53	36.61292968137991	38.0	38.0	38.0	34.0	38.0
54-55	36.6016961115678	38.0	38.0	38.0	34.0	38.0
56-57	36.590561287707075	38.0	38.0	38.0	34.0	38.0
58-59	36.578578405262306	38.0	38.0	38.0	34.0	38.0
60-61	36.58396115811182	38.0	38.0	38.0	34.0	38.0
62-63	36.57156921532233	38.0	38.0	38.0	34.0	38.0
64-65	36.56558692732929	38.0	38.0	38.0	34.0	38.0
66-67	36.5377048008468	38.0	38.0	38.0	34.0	38.0
68-69	36.52302843656381	38.0	38.0	38.0	34.0	38.0
70-71	36.5280197720427	38.0	38.0	38.0	34.0	38.0
72-73	36.511606676243126	38.0	38.0	38.0	34.0	38.0
74-75	36.50958386631582	38.0	38.0	38.0	34.0	38.0
76-77	36.50783834548217	38.0	38.0	38.0	34.0	38.0
78-79	36.50040751754144	38.0	38.0	38.0	34.0	38.0
80-81	36.50291608088273	38.0	38.0	38.0	34.0	38.0
82-83	36.48989331195595	38.0	38.0	38.0	34.0	38.0
84-85	36.488980298818575	38.0	38.0	38.0	34.0	38.0
86-87	36.4815806945808	38.0	38.0	38.0	34.0	38.0
88-89	36.48742525976576	38.0	38.0	38.0	34.0	38.0
90-91	36.497313226300236	38.0	38.0	38.0	34.0	38.0
92-93	36.46968819970628	38.0	38.0	38.0	34.0	38.0
94-95	36.46908696093975	38.0	38.0	38.0	34.0	38.0
96-97	36.46952856228364	38.0	38.0	38.0	34.0	38.0
98-99	36.50221125939382	38.0	38.0	38.0	34.0	38.0
100-101	35.9813783441962	38.0	37.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	11.0
18	94.0
19	189.0
20	289.0
21	708.0
22	1836.0
23	5294.0
24	12234.0
25	23926.0
26	40830.0
27	63727.0
28	92685.0
29	124201.0
30	161583.0
31	204310.0
32	260096.0
33	341897.0
34	485358.0
35	806424.0
36	2026429.0
37	5661731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.102359816681485	24.745245520296393	14.146344159291797	23.006050503730325
2	14.784156297763435	21.30060621385686	42.22098591292565	21.69425157545406
3	17.51448588992246	28.283032875729724	30.391702962392486	23.81077827195533
4	12.739498298017073	19.597265890571244	38.154619631928014	29.50861617948367
5	12.970670299874493	38.23196799263941	35.6725859898045	13.124775717681592
6	28.64123898617122	38.74246983571221	18.7905159003639	13.825775277752678
7	25.540079496971646	33.8241716092106	22.9101891320527	17.72555976176505
8	23.90428910556405	37.336205716351174	21.123746976396404	17.635758201688372
9	25.06355530407068	17.296050011188836	21.40080156279148	36.23959312194901
10-11	23.82259314948479	27.357756345543837	28.627698943130074	20.191951561841297
12-13	25.05593933285062	24.972827804781375	28.876558438108287	21.094674424259725
14-15	22.1121776221553	26.19406325388627	26.82964559737328	24.864113526585154
16-17	21.010598174183613	29.565820800996562	27.52727593919323	21.89630508562659
18-19	21.19936857732688	28.10788830400126	29.826746592834567	20.86599652583729
20-21	22.092051799504798	26.9349076728837	29.855253370715783	21.117787156895716
22-23	21.804602155988555	27.238203156051675	29.738666408609216	21.21852827935055
24-25	21.691951040104428	27.489435549213248	29.78775575911064	21.030857651571687
26-27	21.44462267616751	27.532130814809964	29.900633995700616	21.12261251332191
28-29	20.9026426299663	27.27528426527706	30.157990116520256	21.66408298823638
30-31	21.636271606412212	27.024140429457532	30.278996776739486	21.06059118739077
32-33	21.16085391204381	27.523648258947887	29.951581604319305	21.363916224689
34-35	21.210281514163558	27.617007984694375	30.350540772699762	20.8221697284423
36-37	21.509992898408033	27.428835599616047	29.84083358537484	21.220337916601085
38-39	21.212235664257015	27.278566572883	30.01707059582104	21.492127167038944
40-41	21.352917290151936	27.13363343412581	30.146738111351883	21.36671116437038
42-43	21.657241369166176	27.113932780053013	29.768965890919503	21.459859959861312
44-45	21.319384232323184	27.40244072095462	29.66556763058259	21.612607416139607
46-47	21.720289005225354	27.368317121169984	29.16287087005775	21.748523003546914
48-49	21.589680468143865	27.508316345993144	28.96995079655068	21.932052389312314
50-51	21.3852238817367	27.620186842252487	29.032440709060776	21.96214856695004
52-53	21.53215011104891	27.537179954985213	29.315324833520943	21.61534510044493
54-55	21.537327530080383	27.74288287349013	28.893603983496146	21.826185612933337
56-57	21.73535239080046	27.95811847907953	28.507225078424398	21.799304051695607
58-59	21.24794145067468	28.446310954429237	28.8648680999949	21.44087949490118
60-61	21.615052494843138	28.779481746622835	28.304841121797388	21.30062463673664
62-63	21.297859571344993	28.994205660516165	28.264083634319025	21.443851133819816
64-65	21.32893597122008	28.892987906204198	28.292520184970826	21.485555937604893
66-67	21.44183927049367	28.79538038636939	28.213703794195627	21.549076548941308
68-69	21.4280359079926	28.455807051377697	28.414257929457253	21.701899111172448
70-71	21.55351619180213	28.03239879962396	28.569729640153824	21.84435536842009
72-73	21.692249717255546	27.88759611526792	28.54039091658883	21.8797632508877
74-75	21.519052090214903	28.005548711623728	28.57868316241407	21.896716035747296
76-77	21.700374577938476	27.883218607718646	28.532086973287875	21.884319841055007
78-79	21.656380603766024	27.904887935894156	28.512206755994967	21.92652470434485
80-81	21.616496851802633	27.9788593468865	28.531080759515998	21.873563041794867
82-83	21.655816626654186	27.930202291481454	28.568721190035127	21.84525989182923
84-85	21.843087467744123	27.962146175173046	28.420292225628312	21.774474131454514
86-87	21.651269658954988	28.02186812078866	28.518090754364984	21.808771465891372
88-89	21.727819210584016	27.982499187638567	28.40710397815056	21.882577623626855
90-91	21.881060880278337	27.89611874990512	28.419975450782648	21.80284491903389
92-93	21.835083108227945	27.963396907569148	28.373563727116174	21.827956257086726
94-95	21.66625006836611	27.99660733374527	28.442423324409795	21.894719273478827
96-97	21.766173401006526	28.04714893167261	28.340402092280765	21.846275575040096
98-99	22.03378683367287	28.660777512516027	28.3522782857035	20.953157368107604
100-101	22.548575515191615	31.077895868453638	25.867496625622767	20.506031990731984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4929.0
1	4875.5
2	5213.5
3	5855.5
4	7497.0
5	10689.5
6	13506.5
7	17479.5
8	21280.5
9	22148.5
10	20757.0
11	18895.5
12	19810.0
13	24648.0
14	35844.5
15	49861.0
16	59894.5
17	64478.0
18	63601.0
19	58186.5
20	51106.0
21	44463.5
22	41344.5
23	44919.5
24	54063.0
25	68154.0
26	85379.0
27	105364.5
28	130244.0
29	157124.5
30	184144.5
31	213036.0
32	240825.0
33	269494.0
34	295180.5
35	317341.0
36	342253.5
37	363614.0
38	379402.5
39	389147.0
40	396623.5
41	406059.5
42	412038.0
43	422132.0
44	457267.0
45	476561.5
46	458962.0
47	439861.5
48	419412.0
49	400538.0
50	388062.5
51	373868.5
52	354696.0
53	333957.0
54	314055.0
55	289665.0
56	261555.0
57	231180.5
58	202850.5
59	178200.5
60	151532.5
61	125032.5
62	97387.0
63	74119.0
64	59606.5
65	45685.0
66	31012.5
67	21466.0
68	15770.5
69	11230.5
70	7955.5
71	5458.0
72	3667.0
73	2541.0
74	1744.0
75	1112.5
76	669.0
77	384.0
78	230.5
79	141.0
80	103.0
81	69.5
82	49.0
83	35.5
84	24.0
85	20.0
86	18.5
87	13.5
88	7.5
89	3.5
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018906612194939387
4	0.0
5	0.05791240750788357
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.3934944965275826E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.699684668101282E-6
28-29	9.703955604014954E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	3.451492721294921E-5
40-41	0.0
42-43	0.0
44-45	1.0092003242964322E-5
46-47	2.5996211791239407E-4
48-49	0.0
50-51	4.675027902124862E-5
52-53	1.0479277072400172E-5
54-55	0.0016848142463244905
56-57	0.002332626089623968
58-59	8.434789887234917E-4
60-61	0.0027493108421269255
62-63	0.004647035232573166
64-65	0.0019949430128778822
66-67	0.007270079543387594
68-69	0.004141726485475186
70-71	0.0017144658601681644
72-73	8.067154944278014E-4
74-75	6.62091089906228E-4
76-77	0.0
78-79	0.0
80-81	2.7219339425344093E-4
82-83	0.0
84-85	0.0011259116946220641
86-87	0.001325944735624132
88-89	1.7085664102678007E-4
90-91	7.686383756928874E-4
92-93	3.1127187438779146E-4
94-95	2.6263085336052263E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	175.0
20-21	503.0
22-23	1158.0
24-25	1754.0
26-27	3581.0
28-29	7829.0
30-31	19888.0
32-33	26245.0
34-35	36121.0
36-37	57369.0
38-39	75295.0
40-41	71504.0
42-43	80114.0
44-45	97933.0
46-47	97547.0
48-49	90224.0
50-51	85548.0
52-53	75369.0
54-55	76890.0
56-57	80969.0
58-59	84599.0
60-61	86539.0
62-63	86202.0
64-65	99544.0
66-67	108738.0
68-69	104783.0
70-71	121655.0
72-73	81974.0
74-75	83716.0
76-77	87567.0
78-79	91823.0
80-81	96338.0
82-83	90394.0
84-85	87571.0
86-87	91634.0
88-89	95018.0
90-91	95761.0
92-93	94684.0
94-95	98412.0
96-97	149767.0
98-99	810763.0
100-101	6580354.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.99559457053807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.62612024192948	68.43081873850143
2	8.421813969193574	13.305724037178193
3	2.4687659086112346	5.850648924186575
4	1.0369656249307233	3.276628643624483
5	0.5216670496862101	2.06046993789103
6	0.29263849191501395	1.3870290997831274
7	0.17280330771012528	0.9555490025421896
8	0.11837876144461836	0.7481120515873212
9	0.07620510814526556	0.5417881044522666
>10	0.2595674912068877	3.062764437693289
>50	0.003918621040167141	0.20425742154391888
>100	0.0011053055507830125	0.1281850940108992
>500	0.0	0.0
>1k	5.011863591640758E-5	0.04802450700533662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.695698561507379E-6
2	0.0	9.695698561507379E-6	0.0	9.695698561507379E-6	9.695698561507379E-6
3	0.0	9.695698561507379E-6	0.0	1.9391397123014758E-5	9.695698561507379E-6
4	0.0	1.9391397123014758E-5	0.0	1.9391397123014758E-5	9.695698561507379E-6
5	0.0	1.9391397123014758E-5	0.0	1.9391397123014758E-5	9.695698561507379E-6
6	0.0	1.9391397123014758E-5	0.0	1.9391397123014758E-5	9.695698561507379E-6
7	0.0	1.9391397123014758E-5	0.0	1.9391397123014758E-5	9.695698561507379E-6
8	0.0	1.9391397123014758E-5	0.0	4.847849280753689E-5	3.8782794246029516E-5
9	0.0	6.786988993055165E-5	0.0	1.4543547842261068E-4	3.8782794246029516E-5
10-11	0.0	6.786988993055165E-5	0.0	1.6482687554562545E-4	3.8782794246029516E-5
12-13	0.0	6.786988993055165E-5	0.0	1.8906612194939388E-4	4.36306435267832E-5
14-15	0.0	1.0180483489582747E-4	0.0	2.0360966979165494E-4	7.756558849205903E-5
16-17	0.0	1.1150053345733485E-4	0.0	2.230010669146697E-4	8.726128705356641E-5
18-19	0.0	1.40587629141857E-4	0.0	2.4724031331843817E-4	1.2604408129959592E-4
20-21	0.0	1.6482687554562545E-4	0.0	3.005666554067287E-4	1.5513117698411806E-4
22-23	0.0	1.842182726686402E-4	0.0	3.3450160037200457E-4	1.5513117698411806E-4
24-25	0.0	2.18153217633916E-4	0.0	3.5389299749501933E-4	1.5513117698411806E-4
26-27	0.0	2.4239246403768446E-4	0.0	4.0721933958330994E-4	1.5513117698411806E-4
28-29	0.0	2.4239246403768446E-4	0.0	6.883945978670238E-4	1.745225741071328E-4
30-31	0.0	2.569360118799455E-4	0.0	0.001386484894295555	2.0360966979165494E-4
32-33	0.0	2.617838611606992E-4	0.0	0.0030492971975940707	2.1330536835316232E-4
34-35	0.0	3.2480590181049716E-4	0.0	0.0049399584170880095	2.230010669146697E-4
36-37	0.0	3.878279424602951E-4	0.0	0.008391627104984636	2.230010669146697E-4
38-39	0.0	3.878279424602951E-4	0.0	0.013738804861655955	2.230010669146697E-4
40-41	0.0	4.2661073670632464E-4	0.0	0.019856790653967112	2.278489161954234E-4
42-43	0.0	4.36306435267832E-4	0.0	0.02548029581964139	2.3269676547617708E-4
44-45	0.0	4.896327773561227E-4	0.0	0.031646760104760085	2.4239246403768446E-4
46-47	0.0	5.235677223213984E-4	0.0	0.03800713836110892	2.5208816259919184E-4
48-49	0.0	5.429591194444132E-4	0.0	0.04420268974191214	2.5208816259919184E-4
50-51	0.0	6.253725572172259E-4	0.0	0.05117389700763594	2.617838611606992E-4
52-53	0.0	6.302204064979796E-4	0.0	0.0582759962039401	2.617838611606992E-4
54-55	0.0	6.447639543402408E-4	0.0	0.06501450670418774	2.617838611606992E-4
56-57	0.0	6.593075021825018E-4	0.0	0.07145729839830939	2.7147955972220655E-4
58-59	0.0	6.641553514632555E-4	0.0	0.07742500086291718	2.860231075644677E-4
60-61	0.0	6.786988993055165E-4	0.0	0.08326181139694461	3.005666554067287E-4
62-63	0.0	6.786988993055165E-4	0.0	0.08864777194786197	3.199580525297435E-4
64-65	0.0	7.465687892360682E-4	0.0	0.09425673356569397	3.199580525297435E-4
66-67	0.0	7.465687892360682E-4	0.0	0.09970571615726112	3.199580525297435E-4
68-69	0.0	7.708080356398366E-4	0.0	0.10509652455745924	3.3934944965275824E-4
70-71	0.0	7.853515834820976E-4	0.0	0.11059398564183393	3.684365453372804E-4
72-73	0.0	7.998951313243587E-4	0.0	0.11464193979126325	3.732843946180341E-4
74-75	0.0	8.386779255703883E-4	0.0	0.11826328320398626	3.7813224389878777E-4
76-77	0.0	8.726128705356641E-4	0.0	0.12153073361921424	3.878279424602951E-4
78-79	0.0	8.823085690971715E-4	0.0	0.12447822598191247	4.023714903025562E-4
80-81	0.0	8.823085690971715E-4	0.0	0.12592773291685783	4.2661073670632464E-4
82-83	0.0	8.871564183779251E-4	0.0	0.12636888720140643	4.3630643526783205E-4
84-85	0.0	8.920042676586788E-4	0.0	0.12670338880177842	4.5084998311009314E-4
86-87	0.0	9.0654781550094E-4	0.0	0.12686821567732404	4.750892295138615E-4
88-89	0.0	9.501784590277231E-4	0.0	0.1269360855672546	4.896327773561227E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19400	0.0	52.60492	1
CTTATAC	10600	0.0	39.44261	1
GTATCAA	36055	0.0	36.452824	1
TATACAC	14670	0.0	31.605146	3
TATCAAC	45920	0.0	30.207115	2
TTATACA	14760	0.0	29.825947	2
ATCAACG	46350	0.0	29.779905	3
TCAACGC	46400	0.0	29.756124	4
CAACGCA	47580	0.0	29.0629	5
AACGCAG	49095	0.0	28.245562	6
GGACCGA	3355	0.0	28.15552	6
ACCGAAC	3330	0.0	27.34467	8
CCGAACC	3465	0.0	25.78809	9
GTGGTAT	8525	0.0	25.370518	1
AGGACCG	4115	0.0	25.347996	5
GACCGAA	3635	0.0	25.284388	7
TGGTATC	8890	0.0	24.999355	2
ACGCAGA	56135	0.0	24.680489	7
CGCAGAG	57025	0.0	24.31768	8
TACACAT	21250	0.0	21.637756	5
>>END_MODULE