FastQCFastQC Report
Fri 27 May 2016
SRR2139332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8582031
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135150.052.7210271
GTATCAA240200.035.469961
CTTATAC72600.035.0086481
TCAACGC301750.029.8612124
TATCAAC303100.029.8005752
ATCAACG305950.029.4661733
TATACAC99150.029.314973
CAACGCA311650.028.8847485
AACGCAG321100.028.1022516
TTATACA104150.026.1972942
TGGTATC59900.025.6763042
GTGGTAT61450.024.392331
ACGCAGA376050.023.9842877
CGCAGAG384500.023.4797828
GAGTACT242950.021.1626812-13
GCAGAGT441100.020.427589
TACACAT139850.020.4102765
ACACATC135500.019.7093246
TACATGG305200.019.4314042
GTACATG294550.019.3522241
Produced by FastQC (version 0.11.5)