##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8582031
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.940280453426467	18.0	18.0	32.0	18.0	33.0
2	27.01228322293406	28.0	25.0	31.0	18.0	33.0
3	29.249872436955773	31.0	27.0	33.0	25.0	33.0
4	29.268777169413628	31.0	29.0	33.0	15.0	33.0
5	31.433953687652725	33.0	32.0	33.0	28.0	33.0
6	35.55155580304942	37.0	36.0	38.0	29.0	38.0
7	36.08978166124079	38.0	36.0	38.0	33.0	38.0
8	36.49373557378201	38.0	37.0	38.0	34.0	38.0
9	36.703399230322056	38.0	38.0	38.0	34.0	38.0
10-11	36.83658075809794	38.0	38.0	38.0	35.0	38.0
12-13	36.90710963407147	38.0	38.0	38.0	35.0	38.0
14-15	36.9300361417944	38.0	38.0	38.0	35.0	38.0
16-17	36.956746835335366	38.0	38.0	38.0	35.0	38.0
18-19	36.97013195361331	38.0	38.0	38.0	35.0	38.0
20-21	36.98178228063907	38.0	38.0	38.0	35.0	38.0
22-23	37.00040553031884	38.0	38.0	38.0	36.0	38.0
24-25	37.0051369223014	38.0	38.0	38.0	36.0	38.0
26-27	36.978062265193884	38.0	38.0	38.0	35.5	38.0
28-29	36.948934214054646	38.0	38.0	38.0	35.0	38.0
30-31	36.93971094805016	38.0	38.0	38.0	35.0	38.0
32-33	36.905300278365274	38.0	38.0	38.0	35.0	38.0
34-35	36.88180348982425	38.0	38.0	38.0	35.0	38.0
36-37	36.8551919460635	38.0	38.0	38.0	35.0	38.0
38-39	36.82679280960749	38.0	38.0	38.0	35.0	38.0
40-41	36.843074419317716	38.0	38.0	38.0	35.0	38.0
42-43	36.7946303968134	38.0	38.0	38.0	35.0	38.0
44-45	36.7582074029654	38.0	38.0	38.0	35.0	38.0
46-47	36.714915542935174	38.0	38.0	38.0	35.0	38.0
48-49	36.67907543286533	38.0	38.0	38.0	34.5	38.0
50-51	36.64643251118071	38.0	38.0	38.0	34.0	38.0
52-53	36.62207311308129	38.0	38.0	38.0	34.0	38.0
54-55	36.611321077717506	38.0	38.0	38.0	34.0	38.0
56-57	36.59700968010772	38.0	38.0	38.0	34.0	38.0
58-59	36.58356546510713	38.0	38.0	38.0	34.0	38.0
60-61	36.58524575597782	38.0	38.0	38.0	34.0	38.0
62-63	36.57524288251996	38.0	38.0	38.0	34.0	38.0
64-65	36.5684049237351	38.0	38.0	38.0	34.0	38.0
66-67	36.539992589228646	38.0	38.0	38.0	34.0	38.0
68-69	36.52549415152903	38.0	38.0	38.0	34.0	38.0
70-71	36.53114713833459	38.0	38.0	38.0	34.0	38.0
72-73	36.511705218698246	38.0	38.0	38.0	34.0	38.0
74-75	36.50642459929087	38.0	38.0	38.0	34.0	38.0
76-77	36.500570037671864	38.0	38.0	38.0	34.0	38.0
78-79	36.489080470794065	38.0	38.0	38.0	34.0	38.0
80-81	36.488668621065145	38.0	38.0	38.0	34.0	38.0
82-83	36.470505255698725	38.0	38.0	38.0	34.0	38.0
84-85	36.46479103945636	38.0	38.0	38.0	34.0	38.0
86-87	36.45271387253026	38.0	38.0	38.0	34.0	38.0
88-89	36.45402336868638	38.0	38.0	38.0	34.0	38.0
90-91	36.460522360619926	38.0	38.0	38.0	34.0	38.0
92-93	36.42924060247005	38.0	38.0	38.0	34.0	38.0
94-95	36.42526710704264	38.0	38.0	38.0	34.0	38.0
96-97	36.426292181159084	38.0	38.0	38.0	34.0	38.0
98-99	36.45572754504907	38.0	38.0	38.0	34.0	38.0
100-101	35.93705870887677	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	9.0
18	52.0
19	125.0
20	202.0
21	560.0
22	1627.0
23	4673.0
24	10503.0
25	20054.0
26	34004.0
27	52637.0
28	76186.0
29	102106.0
30	132759.0
31	169209.0
32	215835.0
33	283974.0
34	405339.0
35	671691.0
36	1705674.0
37	4694812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.458638869983105	25.36858699298569	13.675329301420607	23.497444835610594
2	15.339107957079158	21.0490733487213	41.38351399569636	22.228304698503187
3	18.119084399273213	27.412486076741143	29.67626027122549	24.79216925276015
4	13.437098980416174	18.993545933357733	37.23594100277662	30.33341408344948
5	13.857167007689034	37.357712060532926	35.23011723008225	13.555003701695783
6	29.89890155372312	37.83499500293112	18.331045413375925	13.935058029969829
7	26.365413967859126	33.05081279711061	22.545106164263448	18.038667070766813
8	24.234880997283742	37.227900947922464	20.702803334082574	17.834414720711216
9	25.225462364328443	17.374197319958412	21.10486433805704	36.2954759776561
10-11	24.42199288257057	27.008921314779684	28.16150978713547	20.407576015514277
12-13	25.3053443875931	24.367699207798246	28.56837734564231	21.758579058966347
14-15	22.467457575295725	25.709523436651573	26.16519079173361	25.657828196319098
16-17	21.788338914180105	29.06462934007113	26.890563550749235	22.256468194999528
18-19	21.85520537038377	27.758720517322764	28.960836892805442	21.42523721948802
20-21	22.5979477353782	26.793371922755206	28.989456920626594	21.619223421240005
22-23	22.406835050346253	26.908795261606024	28.860086141388546	21.82428354665918
24-25	22.17871459382946	27.16236228028272	28.913014875285274	21.74590825060254
26-27	22.120380937017785	27.201182668477635	28.992558028183428	21.685878366321152
28-29	21.945454684303144	27.02971837524011	29.09466309068281	21.930163849773933
30-31	22.228672734309647	27.21883711327119	28.80806671443585	21.744423437983315
32-33	22.30989851745248	27.050022195819544	28.862331167003425	21.77774811972455
34-35	22.133198116529393	27.035693453324	28.97987810694679	21.85123032319982
36-37	22.03190383636089	27.188765226782923	28.901677698498357	21.877653238357826
38-39	21.958513905935177	27.02287656360967	29.03195374980002	21.98665578065513
40-41	22.1622025600083	26.8875488907994	29.01236088579334	21.937887663398957
42-43	22.206723787082623	26.947657023437642	28.747142347508692	22.098476841971042
44-45	22.05811152275513	27.109920011885354	28.654432497392268	22.177535967967252
46-47	22.421798214702985	27.037281907984156	28.271823257037465	22.26909662027539
48-49	22.222598096872016	27.183549130840078	28.156906389341195	22.436946382946708
50-51	22.104357246257415	27.28489934027226	28.27381748629383	22.336925927176495
52-53	22.312216797069066	27.174418739265505	28.33557513244176	22.177789331223664
54-55	22.22783591004928	27.202201684488532	28.119856860099617	22.45010554536257
56-57	22.389340586431402	27.450871499417257	27.914035878061057	22.245752036090288
58-59	22.125288886944926	27.76834029156565	28.06917333267261	22.03719748881681
60-61	22.363524114451604	27.93817480718157	27.761503923238696	21.93679715512813
62-63	22.102460084271794	28.18599029368472	27.723471326563757	21.98807829547972
64-65	22.166201055643604	28.113996371447357	27.652245235641594	22.067557337267445
66-67	22.212230902367132	27.979974584883717	27.614434089533635	22.19336042321552
68-69	22.152249624402906	27.780830716034753	27.775742119414993	22.29117754014735
70-71	22.272635675826912	27.494644912125267	27.871504537377362	22.36121487467046
72-73	22.3531735664887	27.431159829571207	27.787624825486358	22.42804177845374
74-75	22.140269505712666	27.566405846226722	27.874821653025162	22.41850299503545
76-77	22.44386878173464	27.42408995222328	27.782394844426054	22.349646421616026
78-79	22.28603404870476	27.4726465448338	27.72781409844214	22.5135053080193
80-81	22.38183632381315	27.497091637557496	27.70982115922332	22.411250879406037
82-83	22.358479279245316	27.469315786508503	27.787483630385406	22.384721303860772
84-85	22.3932633582598	27.483734525537358	27.75365277990035	22.369349336302484
86-87	22.366654150251858	27.484492219368118	27.78811737287689	22.360736257503135
88-89	22.375066976142385	27.52602610054662	27.662481625421808	22.43642529788919
90-91	22.451247255371534	27.45789254930317	27.712717751976957	22.378142443348338
92-93	22.454690109995962	27.4119802205681	27.713335781348647	22.41999388808729
94-95	22.364325651581314	27.488708641213318	27.726114209382423	22.420851497822948
96-97	22.455513279658046	27.50955287779381	27.653239886280463	22.38169395626768
98-99	22.663343287273584	28.128790062818382	27.712954902934676	21.49491174697336
100-101	23.337443012577943	30.554228896380646	25.06393340463024	21.044394686411174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2284.0
1	2270.0
2	2783.0
3	3475.5
4	4388.0
5	6076.5
6	7549.5
7	9609.0
8	11729.0
9	12677.5
10	12513.0
11	12194.0
12	13304.5
13	16535.5
14	23793.5
15	32617.5
16	38761.0
17	41522.0
18	40967.0
19	37689.5
20	33290.0
21	29330.5
22	27653.5
23	29726.5
24	35731.5
25	46010.0
26	59170.5
27	74039.0
28	92395.5
29	113129.5
30	133192.5
31	154775.0
32	176852.5
33	197886.5
34	216749.5
35	232947.5
36	251262.5
37	270231.5
38	285820.5
39	296661.5
40	305824.0
41	317262.5
42	327550.0
43	340025.5
44	362605.5
45	374354.5
46	366132.5
47	354699.5
48	345437.5
49	337382.5
50	330246.5
51	323560.0
52	313316.0
53	299904.5
54	285496.5
55	269196.0
56	249747.0
57	226628.5
58	202074.5
59	175641.0
60	147455.5
61	120940.0
62	95960.0
63	74052.0
64	56145.0
65	41667.0
66	30525.5
67	23208.0
68	17889.0
69	12985.5
70	9225.5
71	6366.5
72	4303.5
73	2826.0
74	1789.5
75	1123.5
76	719.5
77	419.0
78	251.5
79	158.5
80	103.5
81	71.5
82	48.5
83	31.0
84	18.0
85	9.5
86	8.0
87	8.0
88	5.5
89	3.5
90	2.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0019575785731839003
4	0.0
5	0.056874648903039386
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.495676023542679E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.7483368217509416E-5
28-29	1.7488561023710408E-5
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	4.132432171881786E-5
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.5445152338006617E-4
48-49	0.0
50-51	4.3052310402237734E-5
52-53	1.8572456601121578E-5
54-55	0.0018693480262426555
56-57	0.0022652867953483436
58-59	7.331941781348348E-4
60-61	0.002725679582609298
62-63	0.004401326839643944
64-65	0.00221114280200283
66-67	0.007203149498823258
68-69	0.004231529521938009
70-71	0.0017453068399307817
72-73	6.862423809435066E-4
74-75	6.646848207012696E-4
76-77	0.0
78-79	0.0
80-81	2.368764853549023E-4
82-83	0.0
84-85	9.937458604274002E-4
86-87	0.0010316801017924368
88-89	1.4469575041603646E-4
90-91	4.678218259641406E-4
92-93	2.216062062147688E-4
94-95	1.4930226572160323E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	94.0
20-21	318.0
22-23	689.0
24-25	1003.0
26-27	1978.0
28-29	4624.0
30-31	12721.0
32-33	16914.0
34-35	23992.0
36-37	38589.0
38-39	46687.0
40-41	46204.0
42-43	53169.0
44-45	64967.0
46-47	66261.0
48-49	60396.0
50-51	54346.0
52-53	51293.0
54-55	55452.0
56-57	57239.0
58-59	58806.0
60-61	58607.0
62-63	58302.0
64-65	69226.0
66-67	76295.0
68-69	73454.0
70-71	83433.0
72-73	60104.0
74-75	61299.0
76-77	64119.0
78-79	67429.0
80-81	68576.0
82-83	65341.0
84-85	64686.0
86-87	67042.0
88-89	70125.0
90-91	71846.0
92-93	70677.0
94-95	75068.0
96-97	123564.0
98-99	716832.0
100-101	5800264.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.43068910696648
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.10479928232013	72.62539318472615
2	7.978867570647728	13.154071042834396
3	2.087012103814173	5.1610153767594635
4	0.8070428919953863	2.661004069042352
5	0.39555496492690656	1.6302934169303434
6	0.21639839039317676	1.0702721065048748
7	0.12346785179261875	0.712427807407566
8	0.07733608916703849	0.5099893698301431
9	0.050888173064008835	0.3775272455754736
>10	0.15572319113602415	1.9062537997388909
>50	0.0025671894723855596	0.1382692759147297
>100	3.17380699889308E-4	0.04089093736213859
>500	2.4920570507997264E-5	0.012592367373466447
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.165225341180893E-5
2	0.0	1.165225341180893E-5	0.0	2.330450682361786E-5	1.165225341180893E-5
3	0.0	1.165225341180893E-5	0.0	3.495676023542679E-5	1.165225341180893E-5
4	0.0	4.660901364723572E-5	0.0	3.495676023542679E-5	1.165225341180893E-5
5	1.165225341180893E-5	4.660901364723572E-5	0.0	5.826126705904465E-5	1.165225341180893E-5
6	1.165225341180893E-5	5.826126705904465E-5	0.0	5.826126705904465E-5	3.495676023542679E-5
7	1.165225341180893E-5	5.826126705904465E-5	0.0	6.991352047085358E-5	3.495676023542679E-5
8	1.165225341180893E-5	8.156577388266251E-5	0.0	8.156577388266251E-5	3.495676023542679E-5
9	1.165225341180893E-5	1.3982704094170717E-4	0.0	1.165225341180893E-4	5.826126705904465E-5
10-11	2.330450682361786E-5	1.3982704094170717E-4	0.0	1.5730542105942056E-4	5.826126705904465E-5
12-13	3.495676023542679E-5	1.3982704094170717E-4	0.0	1.7478380117713394E-4	5.826126705904465E-5
14-15	4.0782886941331256E-5	1.8643605458894285E-4	0.0	1.8643605458894285E-4	1.2234866082399377E-4
16-17	5.2435140353140184E-5	1.980883080007518E-4	0.0	2.0391443470665628E-4	1.7478380117713394E-4
18-19	5.826126705904465E-5	2.2139281482436965E-4	0.0	2.2139281482436965E-4	2.0391443470665628E-4
20-21	5.826126705904465E-5	2.4469732164798753E-4	0.0	2.4469732164798753E-4	2.3887119494208308E-4
22-23	5.826126705904465E-5	2.854802085893188E-4	0.0	2.5634957505979645E-4	2.621757017657009E-4
24-25	8.739190058856697E-5	2.971324620011277E-4	0.0	2.854802085893188E-4	2.971324620011277E-4
26-27	9.321802729447144E-5	3.0295858870703216E-4	0.0	3.2626309553065004E-4	3.087847154129366E-4
28-29	9.321802729447144E-5	3.0295858870703216E-4	0.0	4.719162631782617E-4	3.320892222365545E-4
30-31	1.1069640741218482E-4	3.495676023542679E-4	0.0	0.0010953118207100393	3.495676023542679E-4
32-33	1.2817478752989822E-4	3.5539372906017233E-4	0.0	0.002406190329538544	3.670459824719813E-4
34-35	1.3982704094170714E-4	4.020027427074081E-4	0.0	0.004299681508957495	3.7287210917788576E-4
36-37	1.5147929435351608E-4	4.5443788306054827E-4	0.0	0.0071311790880270644	3.7287210917788576E-4
38-39	1.5147929435351608E-4	4.602640097664527E-4	0.0	0.012007647140869101	3.786982358837902E-4
40-41	1.6313154776532502E-4	5.243514035314018E-4	0.0	0.017140464768770937	3.8452436258969467E-4
42-43	1.6313154776532502E-4	5.301775302373063E-4	0.0	0.021830496767024032	3.9035048929559913E-4
44-45	1.6313154776532502E-4	6.000910507081599E-4	0.0	0.0272720991103388	4.020027427074081E-4
46-47	1.6313154776532502E-4	6.64178444473109E-4	0.0	0.033966318695423034	4.3695950294283484E-4
48-49	1.6313154776532502E-4	6.700045711790135E-4	0.0	0.039786619274621585	4.660901364723572E-4
50-51	1.6313154776532502E-4	7.340919649439626E-4	0.0	0.045286482884995406	4.7191626317826164E-4
52-53	1.6313154776532502E-4	7.340919649439626E-4	0.0	0.05121165374490025	5.01046896707784E-4
54-55	1.6313154776532502E-4	7.399180916498671E-4	0.0	0.05735239129292355	5.301775302373063E-4
56-57	1.6313154776532502E-4	7.573964717675804E-4	0.0	0.06255512244129624	5.476559103550196E-4
58-59	1.6313154776532502E-4	7.632225984734849E-4	0.0	0.06739080760719694	5.593081637668287E-4
60-61	1.6313154776532502E-4	8.098316121207207E-4	0.0	0.07197596932474376	5.651342904727331E-4
62-63	1.6313154776532502E-4	8.506144990620519E-4	0.0	0.07656113104229056	5.942649240022554E-4
64-65	1.6313154776532502E-4	9.321802729447144E-4	0.0	0.08069768100348274	5.942649240022554E-4
66-67	1.6313154776532502E-4	9.554847797683322E-4	0.0	0.0852012769471469	6.000910507081599E-4
68-69	1.7478380117713394E-4	9.787892865919501E-4	0.0	0.08969904676410514	6.117433041199688E-4
70-71	1.7478380117713394E-4	9.787892865919501E-4	0.0	0.09429003460835786	6.408739376494912E-4
72-73	1.7478380117713394E-4	9.787892865919501E-4	0.0	0.09822267013484337	6.874829512967268E-4
74-75	1.7478380117713394E-4	9.962676667096635E-4	0.0	0.10124060376850189	6.874829512967268E-4
76-77	1.7478380117713394E-4	0.0010428766803568992	0.0	0.10392644817992384	6.933090780026313E-4
78-79	1.7478380117713394E-4	0.0010720073138864215	0.0	0.10670551061864028	6.991352047085357E-4
80-81	1.7478380117713394E-4	0.0010720073138864215	0.0	0.10776003955240898	7.049613314144402E-4
82-83	1.806099278830384E-4	0.0010720073138864215	0.0	0.10836595672982305	7.107874581203447E-4
84-85	1.8643605458894288E-4	0.001077833440592326	0.0	0.10873300271229502	7.224397115321536E-4
86-87	1.8643605458894288E-4	0.0010953118207100393	0.0	0.10883787299300131	7.51570345061676E-4
88-89	1.8643605458894288E-4	0.001165225341180893	0.0	0.10889030813335444	7.690487251793893E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13515	0.0	52.721027	1
GTATCAA	24020	0.0	35.46996	1
CTTATAC	7260	0.0	35.008648	1
TCAACGC	30175	0.0	29.861212	4
TATCAAC	30310	0.0	29.800575	2
ATCAACG	30595	0.0	29.466173	3
TATACAC	9915	0.0	29.31497	3
CAACGCA	31165	0.0	28.884748	5
AACGCAG	32110	0.0	28.102251	6
TTATACA	10415	0.0	26.197294	2
TGGTATC	5990	0.0	25.676304	2
GTGGTAT	6145	0.0	24.39233	1
ACGCAGA	37605	0.0	23.984287	7
CGCAGAG	38450	0.0	23.479782	8
GAGTACT	24295	0.0	21.16268	12-13
GCAGAGT	44110	0.0	20.42758	9
TACACAT	13985	0.0	20.410276	5
ACACATC	13550	0.0	19.709324	6
TACATGG	30520	0.0	19.431404	2
GTACATG	29455	0.0	19.352224	1
>>END_MODULE