FastQCFastQC Report
Fri 27 May 2016
SRR2139331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10054768
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147350.051.9503861
GTATCAA270650.038.196941
TCAACGC341900.030.0464484
ATCAACG342350.029.9945893
TATCAAC353500.029.4041062
CAACGCA353150.029.1015575
AACGCAG361900.028.3936716
ACGCAGA413600.024.8025677
CGCAGAG418150.024.5326828
TGGTATC66100.024.0664942
GTGGTAT70950.023.6432271
GCAGAGT480800.021.3359859
GAGTACT288400.020.00052312-13
GTACATG330800.019.8515911
TACATGG331550.019.715172
AGAGTAC439150.019.47779310-11
CAGAGTA471550.018.95711110-11
ACATGGG336450.018.8611763
AGTACTT309400.018.06895812-13
GTACTTT318950.018.03763414-15
Produced by FastQC (version 0.11.5)