##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10054768
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.505909932481785	32.0	28.0	33.0	18.0	34.0
2	31.34033286496516	33.0	31.0	33.0	27.0	34.0
3	31.688463125156144	33.0	31.0	33.0	29.0	34.0
4	32.15043887636194	33.0	33.0	34.0	30.0	34.0
5	32.400984885976484	33.0	33.0	34.0	31.0	34.0
6	35.80361864142465	38.0	36.0	38.0	31.0	38.0
7	36.13927631149719	38.0	37.0	38.0	33.0	38.0
8	36.3309815800822	38.0	37.0	38.0	33.0	38.0
9	36.734182230758584	38.0	38.0	38.0	34.0	38.0
10-11	36.81032675244222	38.0	38.0	38.0	35.0	38.0
12-13	36.87689248523685	38.0	38.0	38.0	35.0	38.0
14-15	36.87353487420097	38.0	38.0	38.0	35.0	38.0
16-17	36.88460653691861	38.0	38.0	38.0	35.0	38.0
18-19	36.910187932730025	38.0	38.0	38.0	35.0	38.0
20-21	36.925362914267794	38.0	38.0	38.0	35.0	38.0
22-23	36.94765850561153	38.0	38.0	38.0	35.0	38.0
24-25	36.938770781920255	38.0	38.0	38.0	35.0	38.0
26-27	36.91942725913188	38.0	38.0	38.0	35.0	38.0
28-29	36.89965556901576	38.0	38.0	38.0	35.0	38.0
30-31	36.893825024889765	38.0	38.0	38.0	35.0	38.0
32-33	36.86886166438753	38.0	38.0	38.0	35.0	38.0
34-35	36.845219472506585	38.0	38.0	38.0	35.0	38.0
36-37	36.81975075965693	38.0	38.0	38.0	35.0	38.0
38-39	36.795960359263646	38.0	38.0	38.0	35.0	38.0
40-41	36.79433003579972	38.0	38.0	38.0	35.0	38.0
42-43	36.74918037689487	38.0	38.0	38.0	35.0	38.0
44-45	36.71069801233965	38.0	38.0	38.0	35.0	38.0
46-47	36.66404291496822	38.0	38.0	38.0	34.0	38.0
48-49	36.628365552825876	38.0	38.0	38.0	34.0	38.0
50-51	36.59609133308251	38.0	38.0	38.0	34.0	38.0
52-53	36.56563845380474	38.0	38.0	38.0	34.0	38.0
54-55	36.54896804846571	38.0	38.0	38.0	34.0	38.0
56-57	36.529398987522285	38.0	38.0	38.0	34.0	38.0
58-59	36.51380211955042	38.0	38.0	38.0	34.0	38.0
60-61	36.51129051484261	38.0	38.0	38.0	34.0	38.0
62-63	36.495150764972124	38.0	38.0	38.0	34.0	38.0
64-65	36.48266069164282	38.0	38.0	38.0	34.0	38.0
66-67	36.45589979367263	38.0	38.0	38.0	34.0	38.0
68-69	36.44423892625127	38.0	38.0	38.0	34.0	38.0
70-71	36.44662702134919	38.0	38.0	38.0	34.0	38.0
72-73	36.42827828988751	38.0	38.0	38.0	34.0	38.0
74-75	36.41538440177622	38.0	38.0	38.0	34.0	38.0
76-77	36.40110553931092	38.0	38.0	38.0	34.0	38.0
78-79	36.38516886604605	38.0	38.0	38.0	34.0	38.0
80-81	36.383040249747495	38.0	38.0	38.0	34.0	38.0
82-83	36.36128397269009	38.0	38.0	38.0	34.0	38.0
84-85	36.348373591224146	38.0	38.0	38.0	34.0	38.0
86-87	36.33353452981271	38.0	38.0	38.0	33.5	38.0
88-89	36.33347409649576	38.0	38.0	38.0	33.5	38.0
90-91	36.34186241780606	38.0	38.0	38.0	34.0	38.0
92-93	36.31345602504297	38.0	37.0	38.0	33.0	38.0
94-95	36.30081953948661	38.0	37.0	38.0	33.0	38.0
96-97	36.2904912714244	38.0	37.0	38.0	33.0	38.0
98-99	36.318801312120684	38.0	37.0	38.0	33.0	38.0
100-101	35.76762356769426	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	8.0
19	23.0
20	76.0
21	400.0
22	1737.0
23	5386.0
24	12714.0
25	24267.0
26	40739.0
27	62151.0
28	89393.0
29	120726.0
30	155536.0
31	197050.0
32	250345.0
33	326928.0
34	452936.0
35	702147.0
36	1500153.0
37	6112052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.64289379924032	20.366447042835798	16.017206960916454	23.97345219700743
2	14.20419645684515	21.607460261639055	41.78313214188532	22.40521113963047
3	17.649421227896354	26.390418598045347	31.19843509841602	24.76172507564228
4	12.43890460724703	18.75354060879376	38.2226919606698	30.58486282328941
5	13.406623392015735	37.2693502204583	35.52923875086465	13.79478763666131
6	29.45252441428783	38.37037314038474	18.624179095927424	13.552923349400006
7	26.04713505075403	32.99239723880253	22.892502343166942	18.0679653672765
8	24.147876907751627	37.00731831903033	20.942034664549197	17.902770108668843
9	25.114393489735416	17.243938398180845	21.300451686204994	36.34121642587875
10-11	24.025074472131035	27.091888146996528	28.676385173680785	20.206652207191652
12-13	24.948427452527994	24.533301016990148	28.919130704955105	21.599140825526757
14-15	22.013047745327487	25.90455131517756	26.743646096517033	25.338754842977917
16-17	21.41460648321274	29.123307469650218	27.403252864710552	22.058833182426486
18-19	21.434054967752616	27.964762588256637	29.472902805912575	21.128279638078173
20-21	22.320065160552442	27.00395974976303	29.45432811286075	21.221646976823774
22-23	21.979182922859188	27.044067945638	29.331373290321	21.64537584118181
24-25	21.73520114743149	27.22980715604797	29.417659222059793	21.617332474460753
26-27	21.676413181663246	27.397813814468723	29.361889637307865	21.563883366560162
28-29	21.57700711672776	27.330826250137406	29.38569660783393	21.706470025300902
30-31	21.96489493753111	27.231421328984624	29.315776319507926	21.48790741397634
32-33	21.74272090864435	27.3385135787476	29.31951712801486	21.59924838459318
34-35	21.822329240908882	27.357735434441988	29.34253428625845	21.47740103839069
36-37	21.824208519863046	27.231773004140646	29.329203710998776	21.61481476499753
38-39	21.59044753156411	27.301261283059258	29.444227145471963	21.66406403990467
40-41	21.85902930652692	27.170274176423604	29.31277213087608	21.657924386173395
42-43	21.84901185556905	27.250113016759148	29.138892684328333	21.761982443343474
44-45	21.792559718041737	27.408337807008877	28.992759277731295	21.80634319721809
46-47	21.975744235009532	27.33672600471519	28.634740631423867	22.052789128851416
48-49	21.834549160238602	27.470782595031544	28.655323200545073	22.039345044184778
50-51	21.811129017399825	27.600568022100212	28.606615621366217	21.98168733913375
52-53	21.966359284319843	27.52251143622165	28.553636742613648	21.957492536844864
54-55	21.917593357191425	27.508337853178322	28.461104988291808	22.11296380133844
56-57	21.98634462490544	27.67959480180781	28.316174693844303	22.01788587944245
58-59	21.84373853126748	27.766291423310186	28.48805157924734	21.901918466174994
60-61	22.002652302934	27.816054284963336	28.340423954047107	21.840869458055554
62-63	21.844754421399635	28.01074716883578	28.29096735730748	21.853531052457104
64-65	21.832553617670772	27.958487458504656	28.241939868988275	21.9670190548363
66-67	21.905954353158382	28.00019061510018	28.076604253442998	22.017250778298436
68-69	21.867294530879846	27.89569999931203	28.23473923082536	22.002266238982763
70-71	21.89904599260912	27.591185869498425	28.280161782194313	22.229606355698145
72-73	21.94833054966557	27.56944781035481	28.265580360661918	22.216641279317702
74-75	21.859930678293495	27.652197721213277	28.242611593069128	22.245260007424093
76-77	22.073943830858518	27.583471668006144	28.1433147210319	22.19926978010344
78-79	22.072728273196205	27.657698486058635	28.012827385745908	22.256745854999245
80-81	22.0680891628043	27.695196772869497	28.0064158683346	22.2302981959916
82-83	22.068641362580276	27.6640011087443	28.06448200611435	22.202875522561076
84-85	22.168829541267833	27.678455843553913	28.056489395672646	22.09622521950561
86-87	22.041306347401722	27.6989373183318	28.07560379333412	22.184152540932356
88-89	22.09988118813354	27.74002002327391	28.022231524513014	22.137867264079535
90-91	22.14217229538141	27.756204107745948	28.01955713888696	22.08206645798568
92-93	22.196162804596366	27.749428501262337	27.982183314257508	22.07222537988379
94-95	22.145014550435757	27.78274722243552	27.98722633194975	22.085011895178965
96-97	22.13538148508297	27.791086809310283	28.032815603106886	22.040716102499857
98-99	22.387322188678613	28.35599561795967	28.07832144440077	21.17836074896094
100-101	22.922484968097915	30.885166668084175	25.462752202244342	20.729596161573568
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3439.0
1	3219.5
2	3417.0
3	4056.5
4	5056.5
5	7148.5
6	9136.5
7	12271.5
8	15476.5
9	16494.0
10	15774.5
11	14781.5
12	15901.5
13	19951.5
14	28650.5
15	39246.0
16	46808.0
17	50231.0
18	49332.0
19	45339.5
20	40584.5
21	36399.0
22	35040.5
23	38754.0
24	47592.0
25	61259.5
26	78662.0
27	98842.0
28	121291.0
29	144251.0
30	166735.0
31	190429.5
32	215620.5
33	241417.0
34	265382.0
35	285560.5
36	308719.5
37	330823.0
38	347047.5
39	360068.5
40	373391.0
41	386455.0
42	393877.5
43	400984.5
44	409922.0
45	421971.0
46	426604.5
47	421052.0
48	414815.5
49	404499.5
50	392930.0
51	380515.0
52	363478.5
53	342142.5
54	320834.5
55	297916.5
56	272106.0
57	245432.0
58	218098.5
59	189239.5
60	157322.5
61	126349.0
62	98715.0
63	75481.0
64	56660.5
65	41742.0
66	30572.0
67	22965.0
68	17354.5
69	12726.0
70	9244.5
71	6519.5
72	4484.5
73	3004.5
74	1929.0
75	1242.5
76	788.5
77	485.5
78	321.0
79	219.0
80	137.0
81	78.0
82	47.0
83	30.0
84	21.5
85	20.5
86	19.0
87	12.0
88	5.0
89	1.5
90	0.5
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0020487792458264574
4	0.0
5	0.058967049264587706
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.978212127818364E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.974572720268981E-6
28-29	4.976280063449561E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	2.5224948523447548E-5
40-41	0.0
42-43	0.0
44-45	1.0282340737852546E-5
46-47	2.0738824780572866E-4
48-49	0.0
50-51	8.954014184633246E-5
52-53	4.2424611068400726E-5
54-55	0.001814487153617738
56-57	0.002568370092458637
58-59	7.141910685104555E-4
60-61	0.0027481988936972675
62-63	0.004627164312143449
64-65	0.002231902537966711
66-67	0.007340265433829797
68-69	0.004308081995656957
70-71	0.0017939534542545288
72-73	7.129450408491636E-4
74-75	6.957866523303692E-4
76-77	0.0
78-79	0.0
80-81	1.967322416959321E-4
82-83	0.0
84-85	0.0010331999978606683
86-87	0.0011475183289973096
88-89	9.918025658304303E-5
90-91	4.636034341236435E-4
92-93	2.2803522003973512E-4
94-95	1.9213142404225046E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	523.0
22-23	1143.0
24-25	1346.0
26-27	2779.0
28-29	6038.0
30-31	16769.0
32-33	22243.0
34-35	30318.0
36-37	48118.0
38-39	55689.0
40-41	58036.0
42-43	66734.0
44-45	80354.0
46-47	80729.0
48-49	73835.0
50-51	67170.0
52-53	58456.0
54-55	62705.0
56-57	62857.0
58-59	70603.0
60-61	72385.0
62-63	69552.0
64-65	76141.0
66-67	82734.0
68-69	82788.0
70-71	89904.0
72-73	73277.0
74-75	73880.0
76-77	78491.0
78-79	81201.0
80-81	82716.0
82-83	79040.0
84-85	78066.0
86-87	80894.0
88-89	84523.0
90-91	87758.0
92-93	85792.0
94-95	91859.0
96-97	149025.0
98-99	843653.0
100-101	6744513.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.13680939370762
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.29172222498866	73.4029208166106
2	8.075940012360764	13.42815770965307
3	2.009032302192495	5.010736067195373
4	0.7545458200540147	2.5092212808259773
5	0.3376416442362044	1.403522451012168
6	0.18102584795042068	0.9029946849833054
7	0.10014249903208929	0.582786949796825
8	0.07000201012174896	0.46557950181345875
9	0.041741501525824606	0.31232297305436724
>10	0.1349058699301572	1.7164531465214434
>50	0.0027224234958584123	0.15163277006852313
>100	5.161035813968895E-4	0.06922162410310395
>500	4.116143883748892E-5	0.025681670647374928
>1k	2.0579091534762714E-5	0.01876835371432089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.94553031954591E-6	0.0
3	0.0	9.94553031954591E-6	0.0	1.989106063909182E-5	0.0
4	0.0	4.9727651597729556E-5	0.0	2.983659095863773E-5	0.0
5	0.0	4.9727651597729556E-5	0.0	2.983659095863773E-5	0.0
6	0.0	4.9727651597729556E-5	0.0	3.978212127818364E-5	0.0
7	0.0	4.9727651597729556E-5	0.0	6.961871223682137E-5	9.94553031954591E-6
8	0.0	7.956424255636728E-5	0.0	6.961871223682137E-5	1.989106063909182E-5
9	0.0	1.3923742447364274E-4	0.0	9.945530319545911E-5	1.989106063909182E-5
10-11	9.94553031954591E-6	1.3923742447364274E-4	0.0	1.1437359867477798E-4	1.989106063909182E-5
12-13	9.94553031954591E-6	1.3923742447364274E-4	0.0	1.2929189415409684E-4	1.989106063909182E-5
14-15	9.94553031954591E-6	1.9393784123114527E-4	0.0	1.3923742447364274E-4	1.989106063909182E-5
16-17	9.94553031954591E-6	1.9891060639091822E-4	0.0	1.3923742447364274E-4	1.989106063909182E-5
18-19	9.94553031954591E-6	2.486382579886478E-4	0.0	1.5415571995296162E-4	4.475488643795659E-5
20-21	9.94553031954591E-6	2.8842037926683143E-4	0.0	1.5912848511273457E-4	6.961871223682137E-5
22-23	9.94553031954591E-6	3.4312079602433394E-4	0.0	1.8399231091159937E-4	6.961871223682137E-5
24-25	9.94553031954591E-6	3.6798462182319875E-4	0.0	2.3869272766910188E-4	6.961871223682137E-5
26-27	9.94553031954591E-6	3.8787568246229055E-4	0.0	3.1328420506569624E-4	6.961871223682137E-5
28-29	9.94553031954591E-6	3.8787568246229055E-4	0.0	7.558603042854893E-4	7.459147739659433E-5
30-31	9.94553031954591E-6	4.674399250186578E-4	0.0	0.0019244601168321338	7.956424255636728E-5
32-33	9.94553031954591E-6	4.7241269017843075E-4	0.0	0.004555052886352027	7.956424255636728E-5
34-35	9.94553031954591E-6	5.420314024152522E-4	0.0	0.00817522592266674	8.95097728759132E-5
36-37	9.94553031954591E-6	6.066773494923006E-4	0.0	0.013918769682204502	8.95097728759132E-5
38-39	9.94553031954591E-6	6.265684101313924E-4	0.0	0.023735008107596316	8.95097728759132E-5
40-41	9.94553031954591E-6	7.160781830073056E-4	0.0	0.05502861925804753	8.95097728759132E-5
42-43	9.94553031954591E-6	7.558603042854893E-4	0.0	0.06541672567681323	8.95097728759132E-5
44-45	9.94553031954591E-6	8.90124963599359E-4	0.0	0.07960899744280525	8.95097728759132E-5
46-47	9.94553031954591E-6	9.84607501635045E-4	0.0	0.09559643743147529	8.95097728759132E-5
48-49	9.94553031954591E-6	9.84607501635045E-4	0.0	0.1112606476847601	8.95097728759132E-5
50-51	9.94553031954591E-6	0.001014444092593683	0.0	0.12614910657312034	8.95097728759132E-5
52-53	9.94553031954591E-6	0.001014444092593683	0.0	0.15304679332233226	8.95097728759132E-5
54-55	9.94553031954591E-6	0.001019416857753456	0.0	0.21725016430016086	8.95097728759132E-5
56-57	9.94553031954591E-6	0.0010343351532327747	0.0	0.28854469839582575	9.448253803568616E-5
58-59	9.94553031954591E-6	0.0010343351532327747	0.0	0.36696520496544527	9.945530319545911E-5
60-61	9.94553031954591E-6	0.0010442806835523207	0.0	0.3862843976111632	9.945530319545911E-5
62-63	9.94553031954591E-6	0.0010641717441914125	0.0	0.3988654934653888	9.945530319545911E-5
64-65	9.94553031954591E-6	0.0011934636383455093	0.0	0.40908949863388194	9.945530319545911E-5
66-67	9.94553031954591E-6	0.0011934636383455093	0.0	0.41555409334158677	9.945530319545911E-5
68-69	9.94553031954591E-6	0.00122827299446392	0.0	0.4220534974054101	9.945530319545911E-5
70-71	9.94553031954591E-6	0.001233245759623693	0.0	0.42821972620352855	9.945530319545911E-5
72-73	1.4918295479318866E-5	0.001238218524783466	0.0	0.43234712128614006	9.945530319545911E-5
74-75	1.989106063909182E-5	0.0012630823505823308	0.0	0.4356739011780282	1.0442806835523206E-4
76-77	1.989106063909182E-5	0.001337673827978925	0.0	0.4386724785693713	1.0940083351500503E-4
78-79	1.989106063909182E-5	0.0013625376537777898	0.0	0.4416511649000753	1.0940083351500503E-4
80-81	1.989106063909182E-5	0.0013675104189375628	0.0	0.4427600915307046	1.1437359867477798E-4
82-83	1.989106063909182E-5	0.0013774559492571088	0.0	0.4431976948647647	1.1934636383455093E-4
84-85	1.989106063909182E-5	0.0013874014795766546	0.0	0.44354081566078896	1.1934636383455093E-4
86-87	1.989106063909182E-5	0.0013973470098962004	0.0	0.44371486244138103	1.1934636383455093E-4
88-89	1.989106063909182E-5	0.0014222108356950654	0.0	0.44378945391877767	1.1934636383455093E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14735	0.0	51.950386	1
GTATCAA	27065	0.0	38.19694	1
TCAACGC	34190	0.0	30.046448	4
ATCAACG	34235	0.0	29.994589	3
TATCAAC	35350	0.0	29.404106	2
CAACGCA	35315	0.0	29.101557	5
AACGCAG	36190	0.0	28.393671	6
ACGCAGA	41360	0.0	24.802567	7
CGCAGAG	41815	0.0	24.532682	8
TGGTATC	6610	0.0	24.066494	2
GTGGTAT	7095	0.0	23.643227	1
GCAGAGT	48080	0.0	21.335985	9
GAGTACT	28840	0.0	20.000523	12-13
GTACATG	33080	0.0	19.851591	1
TACATGG	33155	0.0	19.71517	2
AGAGTAC	43915	0.0	19.477793	10-11
CAGAGTA	47155	0.0	18.957111	10-11
ACATGGG	33645	0.0	18.861176	3
AGTACTT	30940	0.0	18.068958	12-13
GTACTTT	31895	0.0	18.037634	14-15
>>END_MODULE