FastQCFastQC Report
Fri 27 May 2016
SRR2139330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10628103
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153950.053.1816251
GTATCAA284050.037.986931
TCAACGC353350.030.4007534
ATCAACG354950.030.2152353
TATCAAC365700.029.481772
CAACGCA365400.029.4101245
AACGCAG377650.028.4844046
TGGTATC68050.025.5849362
ACGCAGA428950.025.077837
GTGGTAT73500.024.8129961
CGCAGAG433250.024.798938
GCAGAGT498950.021.5683639
GAGTACT292350.020.46595612-13
GTACATG335350.019.9882561
CAGAGTA486950.019.4959510-11
TACATGG343000.019.3394152
AGAGTAC455200.019.28861410-11
ACATGGG345050.018.6064053
GTACTTT321600.018.52356714-15
AGTACTT315700.017.75351712-13