##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139330_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10628103
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.39109547583421	32.0	28.0	33.0	18.0	34.0
2	31.285953288183226	33.0	31.0	33.0	27.0	34.0
3	31.65562781994115	33.0	31.0	33.0	29.0	34.0
4	32.128290815397634	33.0	33.0	34.0	30.0	34.0
5	32.38315116065397	33.0	33.0	34.0	31.0	34.0
6	35.74384826718371	38.0	36.0	38.0	31.0	38.0
7	36.08831218515665	38.0	37.0	38.0	33.0	38.0
8	36.26577668658273	38.0	37.0	38.0	33.0	38.0
9	36.69897262004329	38.0	38.0	38.0	34.0	38.0
10-11	36.78383861165064	38.0	38.0	38.0	34.5	38.0
12-13	36.85386474895849	38.0	38.0	38.0	35.0	38.0
14-15	36.852114671828076	38.0	38.0	38.0	35.0	38.0
16-17	36.86410204154025	38.0	38.0	38.0	35.0	38.0
18-19	36.89001169823062	38.0	38.0	38.0	35.0	38.0
20-21	36.90599317868453	38.0	38.0	38.0	35.0	38.0
22-23	36.92918403882295	38.0	38.0	38.0	35.0	38.0
24-25	36.919130902030744	38.0	38.0	38.0	35.0	38.0
26-27	36.89971503813325	38.0	38.0	38.0	35.0	38.0
28-29	36.87828513310296	38.0	38.0	38.0	35.0	38.0
30-31	36.87620488465314	38.0	38.0	38.0	35.0	38.0
32-33	36.85347516426738	38.0	38.0	38.0	35.0	38.0
34-35	36.83373109958919	38.0	38.0	38.0	35.0	38.0
36-37	36.80830944404045	38.0	38.0	38.0	35.0	38.0
38-39	36.78901385907456	38.0	38.0	38.0	35.0	38.0
40-41	36.77675354587274	38.0	38.0	38.0	35.0	38.0
42-43	36.72878334894665	38.0	38.0	38.0	35.0	38.0
44-45	36.69113474217947	38.0	38.0	38.0	34.5	38.0
46-47	36.64349392710801	38.0	38.0	38.0	34.0	38.0
48-49	36.60808546371364	38.0	38.0	38.0	34.0	38.0
50-51	36.574692446766996	38.0	38.0	38.0	34.0	38.0
52-53	36.54284493778809	38.0	38.0	38.0	34.0	38.0
54-55	36.525950511504334	38.0	38.0	38.0	34.0	38.0
56-57	36.50429041421692	38.0	38.0	38.0	34.0	38.0
58-59	36.489218952488066	38.0	38.0	38.0	34.0	38.0
60-61	36.48283827868113	38.0	38.0	38.0	34.0	38.0
62-63	36.46391756102514	38.0	38.0	38.0	34.0	38.0
64-65	36.45290239301153	38.0	38.0	38.0	34.0	38.0
66-67	36.42239431220477	38.0	38.0	38.0	34.0	38.0
68-69	36.41176781140542	38.0	38.0	38.0	34.0	38.0
70-71	36.41347193504423	38.0	38.0	38.0	34.0	38.0
72-73	36.39204818209956	38.0	38.0	38.0	34.0	38.0
74-75	36.379849576296465	38.0	38.0	38.0	34.0	38.0
76-77	36.36464567456819	38.0	38.0	38.0	34.0	38.0
78-79	36.34982521470303	38.0	38.0	38.0	34.0	38.0
80-81	36.34777036126243	38.0	38.0	38.0	34.0	38.0
82-83	36.32428564540747	38.0	38.0	38.0	33.5	38.0
84-85	36.31211533781165	38.0	37.5	38.0	33.0	38.0
86-87	36.29442543652792	38.0	37.0	38.0	33.0	38.0
88-89	36.293706051244286	38.0	37.0	38.0	33.0	38.0
90-91	36.301359315557	38.0	37.0	38.0	33.0	38.0
92-93	36.27117643704126	38.0	37.0	38.0	33.0	38.0
94-95	36.261519404694226	38.0	37.0	38.0	33.0	38.0
96-97	36.25160585658888	38.0	37.0	38.0	33.0	38.0
98-99	36.27869099856765	38.0	37.0	38.0	33.0	38.0
100-101	35.72817178229934	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	11.0
19	24.0
20	95.0
21	475.0
22	2006.0
23	6129.0
24	14210.0
25	26829.0
26	44261.0
27	67028.0
28	95950.0
29	129612.0
30	167341.0
31	212488.0
32	269434.0
33	351946.0
34	484836.0
35	758020.0
36	1612171.0
37	6385233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71821688216608	20.096361504964715	16.065275242439782	24.120146370429417
2	14.338852380335418	21.475761008337987	41.635407560502564	22.549979050824028
3	17.788782989367412	26.029013838489547	31.094100110520483	25.088103061622558
4	12.586564130964858	18.620519579081986	37.95710297500881	30.83581331494435
5	13.625288605546121	37.126215570219784	35.321835431478235	13.926660392755855
6	29.92827600560514	38.0640929053849	18.458571581400747	13.549059507609213
7	26.420970891983263	32.89329243421897	22.5767100676386	18.109026606159162
8	24.435941202301105	36.989554956326636	20.677801109003177	17.89670273236908
9	25.365401520854665	17.237742238666677	20.96628156501682	36.43057467546184
10-11	24.23989963213567	26.962088154395946	28.466627581610755	20.33138463185763
12-13	25.199041879771105	24.338902452248647	28.70089933739348	21.76115633058676
14-15	22.233730499041716	25.758236456103933	26.56134335212048	25.44668969273387
16-17	21.64456817928844	28.973223161273467	27.16341759201995	22.218791067418145
18-19	21.68334744215407	27.816252815765903	29.247627728109144	21.252772013970887
20-21	22.509245386316174	26.839417518197557	29.26072147016121	21.39061562532506
22-23	22.17749015677087	26.80592507722237	29.149564951950214	21.86701981405655
24-25	21.96726365643986	27.00419429343453	29.21511056134154	21.813431488784065
26-27	21.854619271394093	27.147725260254212	29.176817934459947	21.820837533891748
28-29	21.714383237564352	27.12490142582396	29.2128472459258	21.947868090685887
30-31	22.069344306666256	26.936775417361865	29.16543366202567	21.828446613946216
32-33	21.81282125449171	27.096329523467155	29.168383233668603	21.922465988372533
34-35	21.975906373210886	27.22321518965783	29.1793132029555	21.621565234175787
36-37	21.94108699842799	27.13278511561213	29.067782880567243	21.858345005392636
38-39	21.855795368132608	27.17071197333809	29.154745741527183	21.81874691700212
40-41	22.138250143239606	27.048419903458065	29.025392682776495	21.787937270525834
42-43	22.03292749018776	27.08616817129566	28.881857413331026	21.999046925185556
44-45	22.02267804682208	27.261727186411903	28.724698476535522	21.990896290230495
46-47	22.211196050128375	27.186882042856553	28.361571771989492	22.24035013502558
48-49	22.044655671429407	27.340127416704984	28.43096537493393	22.184251536931672
50-51	22.03408638489511	27.491941959837867	28.33034007236327	22.14363158290375
52-53	22.243718108134292	27.383177588800805	28.260161359929604	22.112942943135298
54-55	22.11269618384449	27.374753176168394	28.257523198444094	22.255027441543024
56-57	22.19460836073626	27.51251190614385	28.117184335747424	22.17569539737246
58-59	22.096792961848095	27.597089484520087	28.25124605869822	22.054871494933597
60-61	22.270728482214874	27.622033577689365	28.159130788844788	21.948107151250973
62-63	22.143321356279262	27.765528616032576	28.08671753871565	22.004432488972515
64-65	22.045122436815692	27.69543915139859	28.106207071567148	22.15323134021858
66-67	22.139523825554193	27.719277574859873	27.955702647657844	22.185495951928093
68-69	22.10697162897851	27.645573574502748	28.04577533068559	22.20167946583316
70-71	22.1288835902329	27.37189738566224	28.10690832649927	22.392310697605588
72-73	22.158838807781848	27.38111653505057	28.059248495517082	22.4007961616505
74-75	22.053179507444966	27.439370247459944	28.07857785127361	22.428872393821482
76-77	22.203398371129424	27.391678416021236	27.965511294255762	22.439411918593578
78-79	22.176358155617383	27.42801189506283	27.929171405091147	22.46645854422864
80-81	22.234017387735573	27.47600539768531	27.87214748442482	22.417829730154292
82-83	22.235010286194505	27.47603154526983	27.94312488807859	22.345833280457075
84-85	22.340892239617855	27.493319974325303	27.885775928666266	22.280011857390576
86-87	22.188991042225325	27.545813230240107	27.88060779751645	22.38458793001812
88-89	22.226058301250863	27.545809016022538	27.863181265169786	22.364951417556817
90-91	22.287460782297423	27.493916776252142	27.917054529764695	22.301567911685737
92-93	22.304747019238096	27.44793695633549	27.925942817273818	22.321373207152597
94-95	22.207995538443942	27.56883398861043	27.902201141539912	22.320969331405717
96-97	22.19277448773509	27.615412480016577	27.874451147979546	22.31736188426879
98-99	22.426921730681258	28.165593818387723	27.943540456087725	21.46394399484329
100-101	23.08918426193728	30.689515939088245	25.26230000166298	20.9589997973115
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4590.0
1	4163.5
2	3763.0
3	4029.5
4	4956.5
5	6819.0
6	8766.5
7	12222.5
8	15896.0
9	17091.0
10	16278.0
11	15229.0
12	16350.0
13	20294.5
14	28946.0
15	39814.5
16	47760.5
17	51380.5
18	50884.5
19	46914.5
20	41714.0
21	37281.0
22	35566.5
23	38567.0
24	46202.0
25	58698.0
26	75595.5
27	94384.0
28	115091.0
29	138018.0
30	159442.0
31	183070.5
32	210550.5
33	237405.0
34	263418.5
35	287500.5
36	313131.0
37	336446.0
38	354781.5
39	371518.0
40	388074.0
41	403658.5
42	413604.5
43	423589.5
44	441009.0
45	451976.0
46	452346.5
47	452122.0
48	447974.5
49	437835.0
50	426127.0
51	412596.5
52	393288.0
53	372548.5
54	350423.5
55	325761.5
56	299705.5
57	271788.0
58	242253.5
59	208870.5
60	172916.5
61	138904.5
62	108599.0
63	82903.5
64	62297.5
65	46232.5
66	34186.5
67	25861.5
68	19353.0
69	14088.5
70	10361.5
71	7435.5
72	5241.0
73	3645.0
74	2502.5
75	1649.5
76	1066.5
77	660.0
78	402.0
79	229.0
80	130.0
81	86.0
82	61.0
83	45.5
84	36.0
85	23.5
86	15.5
87	10.5
88	6.5
89	4.0
90	3.0
91	2.5
92	2.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001966484517509851
4	0.0
5	0.05964375768657869
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.704508415095337E-6
14-15	3.293155890566736E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.7062880903426595E-6
28-29	4.708020126409399E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.4311786953976108E-5
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.840470554310972E-4
48-49	0.0
50-51	6.960296381351854E-5
52-53	2.5021071495359817E-5
54-55	0.001812719307565394
56-57	0.0023520258901277465
58-59	7.706467885387572E-4
60-61	0.002694180072311999
62-63	0.004987816365072109
64-65	0.002279934060341872
66-67	0.007394969085768283
68-69	0.004419256158018595
70-71	0.0018846079199041634
72-73	7.129368054435965E-4
74-75	7.567042362154074E-4
76-77	0.0
78-79	5.537429089067442E-6
80-81	2.3471057587413346E-4
82-83	0.0
84-85	0.001177985667271973
86-87	0.001205955698356024
88-89	1.565349697383118E-4
90-91	5.504619605604546E-4
92-93	2.544214453374637E-4
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	139.0
20-21	586.0
22-23	1229.0
24-25	1507.0
26-27	3064.0
28-29	6632.0
30-31	17133.0
32-33	22116.0
34-35	30929.0
36-37	48992.0
38-39	57613.0
40-41	57967.0
42-43	68399.0
44-45	81301.0
46-47	81620.0
48-49	74777.0
50-51	67552.0
52-53	60766.0
54-55	65178.0
56-57	66104.0
58-59	70855.0
60-61	72931.0
62-63	69064.0
64-65	75714.0
66-67	81063.0
68-69	82156.0
70-71	86897.0
72-73	72898.0
74-75	74498.0
76-77	78618.0
78-79	81707.0
80-81	83040.0
82-83	79457.0
84-85	78591.0
86-87	81605.0
88-89	85528.0
90-91	87887.0
92-93	86645.0
94-95	94082.0
96-97	154314.0
98-99	904845.0
100-101	7232104.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.68709059466939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.13904338730532	74.59787199475586
2	7.4782496651346495	12.516659144313588
3	1.8485243453615277	4.6409287307016625
4	0.701113095293329	2.3469646049168755
5	0.3120270522879283	1.305631809640375
6	0.16491832215328212	0.8280920740057556
7	0.10325411874657658	0.6048725752872255
8	0.06004977209019901	0.4020312573681387
9	0.04159248291137368	0.31326784969152305
>10	0.14529444937704594	1.9180416133934477
>50	0.00446977784753894	0.25293115532525745
>100	0.0013254331238167975	0.1902240755084715
>500	1.1837117560333711E-4	0.06481836428463846
>1k	1.9727191836388544E-5	0.017664750807175895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.409016830190674E-6	0.0	0.0	0.0
3	0.0	9.409016830190674E-6	0.0	0.0	0.0
4	0.0	2.8227050490572023E-5	0.0	9.409016830190674E-6	0.0
5	0.0	2.8227050490572023E-5	0.0	9.409016830190674E-6	0.0
6	9.409016830190674E-6	2.8227050490572023E-5	0.0	2.8227050490572023E-5	9.409016830190674E-6
7	9.409016830190674E-6	2.8227050490572023E-5	0.0	2.8227050490572023E-5	9.409016830190674E-6
8	9.409016830190674E-6	3.76360673207627E-5	0.0	2.8227050490572023E-5	9.409016830190674E-6
9	9.409016830190674E-6	8.468115147171607E-5	0.0	4.704508415095337E-5	9.409016830190674E-6
10-11	1.4113525245286011E-5	8.468115147171607E-5	0.0	6.586311781133472E-5	9.409016830190674E-6
12-13	1.881803366038135E-5	9.879467671700207E-5	0.0	8.468115147171607E-5	1.4113525245286011E-5
14-15	1.881803366038135E-5	1.5995328611324148E-4	0.0	8.468115147171607E-5	1.881803366038135E-5
16-17	4.704508415095337E-5	1.6936230294343214E-4	0.0	8.468115147171607E-5	2.8227050490572023E-5
18-19	5.6454100981144045E-5	1.8347582818871815E-4	0.0	9.879467671700207E-5	2.8227050490572023E-5
20-21	5.6454100981144045E-5	1.9758935343400417E-4	0.0	1.1290820196228809E-4	2.8227050490572023E-5
22-23	5.6454100981144045E-5	2.2111189550948085E-4	0.0	1.1761271037738343E-4	4.704508415095337E-5
24-25	5.6454100981144045E-5	2.493389460000529E-4	0.0	1.4583976086795546E-4	6.586311781133472E-5
26-27	5.6454100981144045E-5	2.540434544151482E-4	0.0	2.587479628302436E-4	6.586311781133472E-5
28-29	5.6454100981144045E-5	2.634524712453389E-4	0.0	6.633356865284425E-4	6.586311781133472E-5
30-31	5.6454100981144045E-5	3.0579304698119693E-4	0.0	0.001754781638830561	6.586311781133472E-5
32-33	5.6454100981144045E-5	3.1990657222648295E-4	0.0	0.003961196085510274	9.409016830190674E-5
34-35	5.6454100981144045E-5	3.7165616479253167E-4	0.0	0.0072026023835109615	1.1290820196228809E-4
36-37	5.6454100981144045E-5	4.3281477418877104E-4	0.0	0.01199649645849311	1.1290820196228809E-4
38-39	5.6454100981144045E-5	4.5163280784915236E-4	0.0	0.02034229438687224	1.1290820196228809E-4
40-41	5.6454100981144045E-5	4.986778920001057E-4	0.0	0.041950101537405124	1.1290820196228809E-4
42-43	5.6454100981144045E-5	5.316094509057732E-4	0.0	0.05048407980238807	1.1761271037738343E-4
44-45	5.6454100981144045E-5	6.068815855472985E-4	0.0	0.06193485328473011	1.2231721879247878E-4
46-47	5.6454100981144045E-5	6.680401949435379E-4	0.0	0.07504631823760083	1.3172623562266947E-4
48-49	5.6454100981144045E-5	6.680401949435379E-4	0.0	0.08682170280058445	1.4113525245286013E-4
50-51	5.6454100981144045E-5	7.574258548303492E-4	0.0	0.09790552462654906	1.4113525245286013E-4
52-53	5.6454100981144045E-5	7.621303632454447E-4	0.0	0.11533572830447729	1.4113525245286013E-4
54-55	5.6454100981144045E-5	7.621303632454447E-4	0.0	0.15363513131176842	1.4113525245286013E-4
56-57	5.6454100981144045E-5	7.621303632454447E-4	0.0	0.1956275734249094	1.4113525245286013E-4
58-59	5.6454100981144045E-5	7.668348716605401E-4	0.0	0.24351946909057995	1.4113525245286013E-4
60-61	6.115860939623938E-5	7.715393800756354E-4	0.0	0.2602110649473382	1.4113525245286013E-4
62-63	7.52721346415254E-5	7.80948396905826E-4	0.0	0.26907435880137787	1.7877131977362282E-4
64-65	7.52721346415254E-5	9.314926661888768E-4	0.0	0.2777776993693042	1.7877131977362282E-4
66-67	7.52721346415254E-5	9.361971746039721E-4	0.0	0.28349367709364504	1.7877131977362282E-4
68-69	7.52721346415254E-5	9.550152082643535E-4	0.0	0.2891720187506651	1.7877131977362282E-4
70-71	7.52721346415254E-5	9.597197166794488E-4	0.0	0.29404588946870386	1.8347582818871815E-4
72-73	8.468115147171607E-5	9.644242250945441E-4	0.0	0.29781420070919523	2.1640738709438552E-4
74-75	8.468115147171607E-5	9.973557840002114E-4	0.0	0.3010556070071959	2.1640738709438552E-4
76-77	8.468115147171607E-5	0.0010491053765662602	0.0	0.30383126697210217	2.2111189550948085E-4
78-79	8.468115147171607E-5	0.0010773324270568322	0.0	0.30613177158708377	2.2581640392457618E-4
80-81	8.468115147171607E-5	0.0010820369354719275	0.0	0.3072420355730463	2.3522542075476687E-4
82-83	8.468115147171607E-5	0.0010820369354719275	0.0	0.30758546468734826	2.4463443758495756E-4
84-85	8.938565988681141E-5	0.0010820369354719275	0.0	0.30797593888580116	2.4463443758495756E-4
86-87	9.879467671700207E-5	0.0010820369354719275	0.0	0.30818764176448044	2.4463443758495756E-4
88-89	1.0349918513209742E-4	0.0010914459523021182	0.0	0.3082299823402163	2.4463443758495756E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15395	0.0	53.181625	1
GTATCAA	28405	0.0	37.98693	1
TCAACGC	35335	0.0	30.400753	4
ATCAACG	35495	0.0	30.215235	3
TATCAAC	36570	0.0	29.48177	2
CAACGCA	36540	0.0	29.410124	5
AACGCAG	37765	0.0	28.484404	6
TGGTATC	6805	0.0	25.584936	2
ACGCAGA	42895	0.0	25.07783	7
GTGGTAT	7350	0.0	24.812996	1
CGCAGAG	43325	0.0	24.79893	8
GCAGAGT	49895	0.0	21.568363	9
GAGTACT	29235	0.0	20.465956	12-13
GTACATG	33535	0.0	19.988256	1
CAGAGTA	48695	0.0	19.49595	10-11
TACATGG	34300	0.0	19.339415	2
AGAGTAC	45520	0.0	19.288614	10-11
ACATGGG	34505	0.0	18.606405	3
GTACTTT	32160	0.0	18.523567	14-15
AGTACTT	31570	0.0	17.753517	12-13
>>END_MODULE