FastQCFastQC Report
Fri 27 May 2016
SRR2139329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13406758
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA196550.052.7810251
GTATCAA375550.039.8765451
TCAACGC470250.031.891574
ATCAACG471750.031.8002243
TATCAAC486250.031.0130942
CAACGCA483450.031.0028025
AACGCAG497250.030.1522316
ACGCAGA565950.026.4613347
CGCAGAG575600.026.0252678
GTGGTAT90250.023.3466741
GCAGAGT656500.022.9308579
TGGTATC87900.022.5345732
GAGTACT399050.021.01576612-13
AGAGTAC597600.020.28016710-11
CAGAGTA641250.019.75810-11
TACATGG427050.019.5216662
GTACATG428650.019.418331
GTACTTT439250.019.07281314-15
AGTACTT431900.018.73733712-13
ACATGGG430550.018.7158553
Produced by FastQC (version 0.11.5)