##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13406758
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.17531374848416	32.0	27.0	33.0	18.0	33.0
2	31.23304843721353	33.0	31.0	33.0	27.0	34.0
3	31.638700347988678	33.0	31.0	33.0	29.0	34.0
4	32.13080999895724	33.0	33.0	34.0	30.0	34.0
5	32.394004277544205	33.0	33.0	34.0	31.0	34.0
6	35.79473001601133	38.0	36.0	38.0	31.0	38.0
7	36.14202210556795	38.0	37.0	38.0	33.0	38.0
8	36.328734210015575	38.0	37.0	38.0	33.0	38.0
9	36.72839697710662	38.0	38.0	38.0	34.0	38.0
10-11	36.8088795963946	38.0	38.0	38.0	35.0	38.0
12-13	36.87987625345367	38.0	38.0	38.0	35.0	38.0
14-15	36.879909184606745	38.0	38.0	38.0	35.0	38.0
16-17	36.89630401324466	38.0	38.0	38.0	35.0	38.0
18-19	36.917993410487455	38.0	38.0	38.0	35.0	38.0
20-21	36.93221777822801	38.0	38.0	38.0	35.0	38.0
22-23	36.95441990224586	38.0	38.0	38.0	35.0	38.0
24-25	36.94841911527698	38.0	38.0	38.0	35.0	38.0
26-27	36.930038614816	38.0	38.0	38.0	35.0	38.0
28-29	36.91153888213833	38.0	38.0	38.0	35.0	38.0
30-31	36.90575720940764	38.0	38.0	38.0	35.0	38.0
32-33	36.88424006832223	38.0	38.0	38.0	35.0	38.0
34-35	36.86521463685831	38.0	38.0	38.0	35.0	38.0
36-37	36.84193539120489	38.0	38.0	38.0	35.0	38.0
38-39	36.83293095376491	38.0	38.0	38.0	35.0	38.0
40-41	36.80865512121288	38.0	38.0	38.0	35.0	38.0
42-43	36.758822437231984	38.0	38.0	38.0	35.0	38.0
44-45	36.72049044943645	38.0	38.0	38.0	35.0	38.0
46-47	36.67402255195303	38.0	38.0	38.0	34.0	38.0
48-49	36.63951477006407	38.0	38.0	38.0	34.0	38.0
50-51	36.6083233572101	38.0	38.0	38.0	34.0	38.0
52-53	36.582975442183155	38.0	38.0	38.0	34.0	38.0
54-55	36.56958350594	38.0	38.0	38.0	34.0	38.0
56-57	36.55257276283866	38.0	38.0	38.0	34.0	38.0
58-59	36.5426924660562	38.0	38.0	38.0	34.0	38.0
60-61	36.5453184342116	38.0	38.0	38.0	34.0	38.0
62-63	36.52916039998169	38.0	38.0	38.0	34.0	38.0
64-65	36.5193750030715	38.0	38.0	38.0	34.0	38.0
66-67	36.49553002239011	38.0	38.0	38.0	34.0	38.0
68-69	36.48610937077773	38.0	38.0	38.0	34.0	38.0
70-71	36.49189046319529	38.0	38.0	38.0	34.0	38.0
72-73	36.47354110106886	38.0	38.0	38.0	34.0	38.0
74-75	36.46305332234175	38.0	38.0	38.0	34.0	38.0
76-77	36.44853078329582	38.0	38.0	38.0	34.0	38.0
78-79	36.43659138744895	38.0	38.0	38.0	34.0	38.0
80-81	36.4338378585018	38.0	38.0	38.0	34.0	38.0
82-83	36.41253407741351	38.0	38.0	38.0	34.0	38.0
84-85	36.40240317619765	38.0	38.0	38.0	34.0	38.0
86-87	36.38401337619871	38.0	38.0	38.0	34.0	38.0
88-89	36.38359258281018	38.0	38.0	38.0	34.0	38.0
90-91	36.38804916196607	38.0	38.0	38.0	34.0	38.0
92-93	36.358812757172146	38.0	38.0	38.0	34.0	38.0
94-95	36.34501385882688	38.0	38.0	38.0	34.0	38.0
96-97	36.33966904235249	38.0	37.0	38.0	33.5	38.0
98-99	36.36816783939548	38.0	37.5	38.0	34.0	38.0
100-101	35.83135702378017	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	12.0
19	31.0
20	101.0
21	556.0
22	2347.0
23	6994.0
24	16491.0
25	31296.0
26	52384.0
27	80559.0
28	116796.0
29	158449.0
30	204164.0
31	259912.0
32	330818.0
33	431391.0
34	597731.0
35	932149.0
36	1990675.0
37	8193900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.803478215986296	20.144385391307875	16.179623739012815	23.872512653693008
2	14.25245387438186	21.568704380283435	41.664576924562965	22.514264820771736
3	17.665743334284116	26.30447800292873	31.11571451246976	24.914064150317397
4	12.535125941707905	18.495478176006458	38.293292084484555	30.67610379780108
5	13.487068827510553	37.2692058848707	35.36259429038247	13.881130997236266
6	29.77813875658828	38.23097276761466	18.514319420101412	13.476569055695641
7	26.32074808838945	32.96460635748031	22.906604266296146	17.808041287834094
8	24.373998546106375	36.919611736110994	20.76181281112108	17.94457690666155
9	25.39856391828658	17.169117246690067	21.19416938830402	36.23814944671933
10-11	24.255539631579833	26.982488980557417	28.594105301221965	20.16786608664078
12-13	25.15668405118404	24.471827899916438	28.847352868408073	21.52413518049145
14-15	22.171966458100147	25.80666058316577	26.809416358351918	25.21195660038217
16-17	21.516048100517665	28.94699076391175	27.418026789176025	22.118934346394557
18-19	21.507302511166383	27.851979576270413	29.550041106134685	21.09067680642852
20-21	22.47009747757425	26.951428370353693	29.485094919676953	21.093379232395105
22-23	22.12843028634696	26.776647431657217	29.40432141531079	21.69060086668503
24-25	21.838162885287385	26.964980588289713	29.466875505435574	21.729981020987328
26-27	21.7547761201547	27.14730336703483	29.395769992626224	21.702150520184247
28-29	21.690998568471638	27.19322438272197	29.450186074802076	21.665590974004314
30-31	22.03928865616373	26.981385992223217	29.31679237951436	21.662532972098692
32-33	21.80395741427283	27.158011276698158	29.265880925864852	21.772150383164163
34-35	21.904256606149872	27.204443143411233	29.262225668310183	21.62907458212871
36-37	21.892888035557	27.11047826091864	29.27248189021291	21.72415181331145
38-39	21.748145220634317	27.271901245413034	29.304986495897158	21.674967038055488
40-41	22.064952872941568	27.08742784837353	29.14503978079398	21.702579497890927
42-43	21.958029690455252	27.18941899021973	28.94332048912547	21.90923083019955
44-45	22.00241782906226	27.319781008484362	28.786970998764506	21.890830163688875
46-47	22.079600585529878	27.320746341622954	28.523684276558846	22.07596879628832
48-49	21.976888248094312	27.478794180837323	28.505942695826036	22.038374875242326
50-51	22.005961885847807	27.613053959283096	28.34560369389079	22.035380460978303
52-53	22.224024909162843	27.505631609885207	28.181583623152978	22.088759857798973
54-55	22.169582796430642	27.414523146468543	28.266557535315656	22.149336521785152
56-57	22.136366911789274	27.559711649825307	28.2408578204337	22.06306361795172
58-59	22.11013984858458	27.593536344326914	28.23854592604388	22.057777881044625
60-61	22.16246845916245	27.533779084495315	28.29012598932333	22.013626467018906
62-63	22.150993250396137	27.5843167650815	28.240683575396126	22.024006409126233
64-65	22.109954089758507	27.532780464408408	28.213297323545678	22.143968122287408
66-67	22.216303660709873	27.588483341444153	28.04670111144182	22.148511886404158
68-69	22.136530308962392	27.53436306764841	28.181836934877513	22.147269688511685
70-71	22.130291516373145	27.31673614001235	28.229988441767954	22.32298390184655
72-73	22.185816778735376	27.390663946700716	28.149173929531244	22.27434534503266
74-75	22.009657652173544	27.545100288381995	28.14664164428551	22.298600415158955
76-77	22.173905517096077	27.48566997634091	28.041999534786378	22.298424971776633
78-79	22.173273739263454	27.5614514484218	27.983384027138335	22.28189078517641
80-81	22.185438337204662	27.532420595671336	27.97016821217876	22.311972854945243
82-83	22.20338389203894	27.53184565642568	28.019360071189404	22.245410380345977
84-85	22.29013762879493	27.491820821097985	27.980102956137255	22.237938593969826
86-87	22.14711391708351	27.548069383531832	28.00878279004811	22.296033909336554
88-89	22.201695757281655	27.55459763344617	27.9631995143458	22.280507094926374
90-91	22.28394144578659	27.572436771091564	27.9736722853493	22.169949497772556
92-93	22.28936617182223	27.55711701078442	27.935838068908787	22.217678748484563
94-95	22.17266030061192	27.67580628006316	27.91092246294584	22.24061095637908
96-97	22.189785765387064	27.668572980781835	27.955838025474435	22.18580322835666
98-99	22.454709323450977	28.256187685741217	27.95833488930658	21.330768101501224
100-101	23.07059192150664	30.753441188476348	25.330191321616013	20.845775568400988
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3684.0
1	3556.0
2	4245.0
3	5390.0
4	6815.5
5	9583.0
6	12321.0
7	16673.0
8	21176.5
9	22763.5
10	21816.0
11	20378.0
12	21717.0
13	27304.0
14	39430.5
15	54109.0
16	64728.0
17	69389.5
18	68082.0
19	62135.0
20	54750.5
21	48729.5
22	46840.5
23	51307.0
24	61688.5
25	78649.5
26	101856.5
27	127760.5
28	155992.0
29	185911.0
30	213566.5
31	244083.0
32	277784.5
33	310959.5
34	343733.5
35	372820.0
36	405015.5
37	433620.5
38	451796.0
39	467105.0
40	488003.5
41	508734.0
42	519010.0
43	527969.5
44	537684.0
45	545564.0
46	548138.5
47	546992.5
48	544954.0
49	536674.0
50	524685.0
51	509555.5
52	486607.0
53	461512.5
54	435673.0
55	405594.0
56	373147.5
57	338694.0
58	303355.5
59	265016.5
60	222507.0
61	180368.0
62	140955.5
63	107329.0
64	81503.5
65	61254.0
66	45189.0
67	34133.5
68	26081.5
69	19011.5
70	13878.5
71	10309.0
72	7271.0
73	4903.5
74	3252.0
75	2074.5
76	1321.0
77	796.0
78	489.0
79	305.0
80	185.5
81	118.0
82	80.0
83	57.5
84	39.5
85	26.5
86	16.5
87	12.5
88	12.5
89	9.0
90	5.0
91	4.0
92	3.5
93	2.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018348955056845212
4	0.0
5	0.05860477230960684
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	7.458924819855778E-6
14-15	2.983569927942311E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.461448467860575E-6
28-29	7.464040120708457E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.512492564681083E-5
40-41	0.0
42-43	0.0
44-45	1.5409998022126755E-5
46-47	2.641294290135457E-4
48-49	0.0
50-51	6.70485912183857E-5
52-53	7.941351530675655E-6
54-55	0.0019506395459550692
56-57	0.002507925022944696
58-59	8.109895901781699E-4
60-61	0.002920788143838726
62-63	0.00463274986331125
64-65	0.0021962769624594525
66-67	0.00734534417510031
68-69	0.004335978560901344
70-71	0.001702208078000353
72-73	6.248230648726827E-4
74-75	7.031132690889106E-4
76-77	0.0
78-79	0.0
80-81	1.4612377463037992E-4
82-83	0.0
84-85	9.919386498569737E-4
86-87	0.0011009758148824724
88-89	1.515600349965899E-4
90-91	4.7778605631028744E-4
92-93	2.999464783002783E-4
94-95	4.735567116127234E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	132.0
20-21	635.0
22-23	1382.0
24-25	1733.0
26-27	3666.0
28-29	8063.0
30-31	21353.0
32-33	28181.0
34-35	38456.0
36-37	61056.0
38-39	75453.0
40-41	75236.0
42-43	87365.0
44-45	104602.0
46-47	104721.0
48-49	95584.0
50-51	86453.0
52-53	82683.0
54-55	86868.0
56-57	90000.0
58-59	90685.0
60-61	88492.0
62-63	85749.0
64-65	91075.0
66-67	95238.0
68-69	98513.0
70-71	97022.0
72-73	92064.0
74-75	94063.0
76-77	98996.0
78-79	102904.0
80-81	104990.0
82-83	99443.0
84-85	98417.0
86-87	102302.0
88-89	106770.0
90-91	110860.0
92-93	108950.0
94-95	117629.0
96-97	194225.0
98-99	1131077.0
100-101	9143672.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.46252691224318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.84023665328631	71.55687642346146
2	8.092646645168747	13.18494890246667
3	2.143895153541718	5.239413499272598
4	0.823434592223941	2.6831625051805914
5	0.4227240840710809	1.721808603754689
6	0.21321732433449256	1.042153321305305
7	0.12694348708232825	0.7238796062944711
8	0.08406230324680322	0.5478342112438284
9	0.05980352491822561	0.43845716322881634
>10	0.1871310666805497	2.34267475107196
>50	0.004496714949156672	0.254291710438424
>100	0.001303303733230507	0.17836210372354383
>500	7.010098203770949E-5	0.04015874563076992
>1k	3.504578141616467E-5	0.0459784529268439
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.458924819855777E-6
2	0.0	7.458924819855777E-6	0.0	0.0	7.458924819855777E-6
3	0.0	7.458924819855777E-6	0.0	0.0	7.458924819855777E-6
4	0.0	6.713032337870199E-5	0.0	7.458924819855777E-6	7.458924819855777E-6
5	0.0	6.713032337870199E-5	0.0	1.4917849639711554E-5	7.458924819855777E-6
6	0.0	6.713032337870199E-5	0.0	1.4917849639711554E-5	2.2376774459567332E-5
7	0.0	6.713032337870199E-5	0.0	3.729462409927889E-5	3.729462409927889E-5
8	0.0	7.458924819855778E-5	0.0	3.729462409927889E-5	4.4753548919134664E-5
9	0.0	1.939320453162502E-4	0.0	8.204817301841355E-5	5.221247373899044E-5
10-11	0.0	1.939320453162502E-4	0.0	8.950709783826933E-5	5.221247373899044E-5
12-13	0.0	1.939320453162502E-4	0.0	9.69660226581251E-5	5.221247373899044E-5
14-15	0.0	2.983569927942311E-4	0.0	1.1934279711769245E-4	6.713032337870199E-5
16-17	0.0	3.0954538002401474E-4	0.0	1.2680172193754822E-4	8.577763542834144E-5
18-19	0.0	3.4684000412329367E-4	0.0	1.3426064675740399E-4	1.0069548506805298E-4
20-21	0.0	3.8786409063250045E-4	0.0	1.5290795880704345E-4	1.0815440988790878E-4
22-23	0.0	4.139703275019956E-4	0.0	1.67825808446755E-4	1.1561333470776455E-4
24-25	0.0	4.512649516012745E-4	0.0	1.976615077261781E-4	1.3426064675740399E-4
26-27	0.0	4.69912263650914E-4	0.0	2.312266694155291E-4	1.3426064675740399E-4
28-29	0.0	4.773711884707698E-4	0.0	3.9159355304242834E-4	1.3799010916733186E-4
30-31	0.0	5.258541997998324E-4	0.0	9.696602265812511E-4	1.4917849639711555E-4
32-33	0.0	5.370425870296159E-4	0.0	0.002140711423298608	1.5663742121697132E-4
34-35	0.0	6.079023728182459E-4	0.0	0.0037070856354683213	1.640963460368271E-4
36-37	0.0	6.750326961969479E-4	0.0	0.006284144160728492	1.67825808446755E-4
38-39	0.0	6.862210834267315E-4	0.0	0.01033434033791018	1.8647312049639443E-4
40-41	0.0	7.160567827061546E-4	0.0	0.01457100963558826	1.939320453162502E-4
42-43	0.0	7.34704094755794E-4	0.0	0.018927021730384033	2.0139097013610596E-4
44-45	0.0	8.35399579823847E-4	0.0	0.02359257920520382	2.0884989495596176E-4
46-47	0.0	9.211772152521885E-4	0.0	0.028925710451400702	2.1630881977581753E-4
48-49	0.0	9.286361400720443E-4	0.0	0.03427748900964722	2.2376774459567333E-4
50-51	0.0	0.001107650335748583	0.0	0.03986795316212913	2.274972070056012E-4
52-53	0.0	0.0011262976477982225	0.0	0.045197354945916084	2.6106236869495223E-4
54-55	0.0	0.0011300271102081504	0.0	0.05065728791405051	2.908980679743753E-4
56-57	0.0	0.0011561333470776454	0.0	0.056393201100519603	2.946275303843032E-4
58-59	0.0	0.001171051196717357	0.0	0.0617412501963562	3.02086455204159E-4
60-61	0.0	0.001204616358406708	0.0	0.06708556982978287	3.1327484243394264E-4
62-63	0.0	0.0012195342080464195	0.0	0.07226952257958262	3.281926920736542E-4
64-65	0.0	0.0013799010916733187	0.0	0.07724462543442642	3.281926920736542E-4
66-67	0.0	0.0013799010916733187	0.0	0.08240993087217655	3.281926920736542E-4
68-69	0.0	0.0014134662533626698	0.0	0.08786240491549113	3.281926920736542E-4
70-71	0.0	0.0014283841030023814	0.0	0.09276291852213638	3.3565161689351E-4
72-73	0.0	0.0014321135654123093	0.0	0.09666020674051101	3.953230154523562E-4
74-75	0.0	0.0014470314150520208	0.0	0.0996400472060434	3.953230154523562E-4
76-77	0.0	0.0015141617384307228	0.0	0.10247070917517867	4.0651140268213983E-4
78-79	0.0	0.001543997437710146	0.0	0.10515592211032675	4.102408650920677E-4
80-81	0.0	0.001543997437710146	0.0	0.10614422964895764	4.3634710196156296E-4
82-83	0.0	0.0015514563625300016	0.0	0.10654701158922986	4.549944140112024E-4
84-85	0.0	0.0015551858249399295	0.0	0.10687520428130351	4.549944140112024E-4
86-87	0.0	0.0015812920618094247	0.0	0.10700573546565098	4.549944140112024E-4
88-89	0.0	0.0016446929227781988	0.0	0.10707286578902968	4.587238764211303E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19655	0.0	52.781025	1
GTATCAA	37555	0.0	39.876545	1
TCAACGC	47025	0.0	31.89157	4
ATCAACG	47175	0.0	31.800224	3
TATCAAC	48625	0.0	31.013094	2
CAACGCA	48345	0.0	31.002802	5
AACGCAG	49725	0.0	30.152231	6
ACGCAGA	56595	0.0	26.461334	7
CGCAGAG	57560	0.0	26.025267	8
GTGGTAT	9025	0.0	23.346674	1
GCAGAGT	65650	0.0	22.930857	9
TGGTATC	8790	0.0	22.534573	2
GAGTACT	39905	0.0	21.015766	12-13
AGAGTAC	59760	0.0	20.280167	10-11
CAGAGTA	64125	0.0	19.758	10-11
TACATGG	42705	0.0	19.521666	2
GTACATG	42865	0.0	19.41833	1
GTACTTT	43925	0.0	19.072813	14-15
AGTACTT	43190	0.0	18.737337	12-13
ACATGGG	43055	0.0	18.715855	3
>>END_MODULE