FastQCFastQC Report
Fri 27 May 2016
SRR2139328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10955718
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159100.053.543551
GTATCAA296200.038.2144281
TCAACGC367550.030.90184
ATCAACG370350.030.6810673
TATCAAC379100.030.1800232
CAACGCA378200.030.066215
AACGCAG388600.029.323796
CTTATAC82750.028.148051
ACGCAGA444450.025.5898957
CGCAGAG448800.025.3515788
TGGTATC70550.024.792622
GTGGTAT75200.024.4196431
GCAGAGT513550.022.1891259
TATACAC133850.021.1169743
GAGTACT323800.021.09645512-13
CAGAGTA504750.019.73886710-11
GTACATG332350.019.3847371
AGAGTAC471650.019.35415310-11
TACATGG328800.019.3419742
GTACTTT356550.019.12249414-15
Produced by FastQC (version 0.11.5)