##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10955718
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.26921585604887	32.0	28.0	33.0	18.0	33.0
2	31.250244392927968	33.0	31.0	33.0	27.0	34.0
3	31.635412485060314	33.0	31.0	33.0	29.0	34.0
4	32.12606476362389	33.0	33.0	34.0	30.0	34.0
5	32.38216564172243	33.0	33.0	34.0	31.0	34.0
6	35.75728190521151	38.0	36.0	38.0	31.0	38.0
7	36.094693109114345	38.0	37.0	38.0	33.0	38.0
8	36.27944129266562	38.0	37.0	38.0	33.0	38.0
9	36.705434185144235	38.0	38.0	38.0	34.0	38.0
10-11	36.78594679965293	38.0	38.0	38.0	34.5	38.0
12-13	36.85652606246345	38.0	38.0	38.0	35.0	38.0
14-15	36.85756643243282	38.0	38.0	38.0	35.0	38.0
16-17	36.86978785872364	38.0	38.0	38.0	35.0	38.0
18-19	36.89571030397095	38.0	38.0	38.0	35.0	38.0
20-21	36.91156187499392	38.0	38.0	38.0	35.0	38.0
22-23	36.93402045775214	38.0	38.0	38.0	35.0	38.0
24-25	36.92498648539869	38.0	38.0	38.0	35.0	38.0
26-27	36.90307783303005	38.0	38.0	38.0	35.0	38.0
28-29	36.88541946406065	38.0	38.0	38.0	35.0	38.0
30-31	36.876376826599014	38.0	38.0	38.0	35.0	38.0
32-33	36.85523888343714	38.0	38.0	38.0	35.0	38.0
34-35	36.833646159225864	38.0	38.0	38.0	35.0	38.0
36-37	36.806059811960246	38.0	38.0	38.0	35.0	38.0
38-39	36.784413460450125	38.0	38.0	38.0	35.0	38.0
40-41	36.77871471444614	38.0	38.0	38.0	35.0	38.0
42-43	36.73267236221396	38.0	38.0	38.0	35.0	38.0
44-45	36.690976931293505	38.0	38.0	38.0	34.5	38.0
46-47	36.64217288505121	38.0	38.0	38.0	34.0	38.0
48-49	36.60558554248584	38.0	38.0	38.0	34.0	38.0
50-51	36.57062624475447	38.0	38.0	38.0	34.0	38.0
52-53	36.543021118287015	38.0	38.0	38.0	34.0	38.0
54-55	36.528872313977864	38.0	38.0	38.0	34.0	38.0
56-57	36.510474446611624	38.0	38.0	38.0	34.0	38.0
58-59	36.49714245304378	38.0	38.0	38.0	34.0	38.0
60-61	36.49528656233845	38.0	38.0	38.0	34.0	38.0
62-63	36.47789370847852	38.0	38.0	38.0	34.0	38.0
64-65	36.46741357069705	38.0	38.0	38.0	34.0	38.0
66-67	36.43987421345655	38.0	38.0	38.0	34.0	38.0
68-69	36.4262418601472	38.0	38.0	38.0	34.0	38.0
70-71	36.43029504037192	38.0	38.0	38.0	34.0	38.0
72-73	36.41107614583729	38.0	38.0	38.0	34.0	38.0
74-75	36.39996045486384	38.0	38.0	38.0	34.0	38.0
76-77	36.38495607437886	38.0	38.0	38.0	34.0	38.0
78-79	36.37468078762867	38.0	38.0	38.0	34.0	38.0
80-81	36.369939060660805	38.0	38.0	38.0	34.0	38.0
82-83	36.35083215742414	38.0	38.0	38.0	33.5	38.0
84-85	36.337891830009866	38.0	38.0	38.0	34.0	38.0
86-87	36.31837139408059	38.0	37.5	38.0	33.0	38.0
88-89	36.320594749485075	38.0	37.5	38.0	33.0	38.0
90-91	36.32653552072365	38.0	38.0	38.0	33.5	38.0
92-93	36.29619531947328	38.0	37.0	38.0	33.0	38.0
94-95	36.28677827068858	38.0	37.0	38.0	33.0	38.0
96-97	36.27928077856226	38.0	37.0	38.0	33.0	38.0
98-99	36.31092759716248	38.0	37.0	38.0	33.0	38.0
100-101	35.75998543040413	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	20.0
20	81.0
21	536.0
22	1918.0
23	6086.0
24	13714.0
25	26690.0
26	44729.0
27	68648.0
28	99230.0
29	134216.0
30	173148.0
31	218230.0
32	279509.0
33	362151.0
34	500964.0
35	779375.0
36	1659008.0
37	6587456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.63744776928358	20.18030219470782	16.12826288518927	24.053987150819324
2	14.267928400493696	21.470486918337986	41.78345955965643	22.47812512151189
3	17.752931855356934	26.062925356350043	31.19306066713401	24.991082121159014
4	12.49958240984297	18.663925084599658	38.01349213260144	30.823000372955928
5	13.515370462139492	37.14007316214675	35.46572234514143	13.878834030572328
6	29.77893370384305	38.17105369086718	18.474215929982865	13.575796675306902
7	26.341395424745322	32.87674983967276	22.62781864228342	18.154036093298494
8	24.28659627785235	37.11542228450933	20.688383910575283	17.90959752706304
9	25.21820112565877	17.233101472673905	21.06832249607009	36.48037490559724
10-11	24.18835077719233	26.99831722576284	28.56075704029622	20.25257495674861
12-13	25.113001498988996	24.422124794656305	28.78424895494065	21.680624751414047
14-15	22.117362683983902	25.84293456364414	26.608895595485606	25.430807156886353
16-17	21.525960233733656	29.034231257138966	27.284537626835593	22.155270882291784
18-19	21.53750215184436	27.833447337728117	29.414416289283825	21.2146342211437
20-21	22.3704758948215	26.893478888110085	29.422644808196495	21.313400408871917
22-23	22.02587430374988	26.93497137864701	29.284342713915954	21.754811603687155
24-25	21.887927067965705	27.09421239829976	29.383106805334425	21.63475372840011
26-27	21.842519274523106	27.21115401617563	29.30289498933846	21.643431719962805
28-29	21.69283234367668	27.14593220861452	29.40940503006646	21.75183041764234
30-31	21.96457317177121	27.063744624966972	29.387654719802658	21.584027483459163
32-33	21.61770461814821	27.113521179502182	29.458192393772887	21.810581808576725
34-35	21.752973736154964	27.17408184355011	29.3487956526277	21.72414876766722
36-37	21.774788730585744	27.16887685525381	29.387640074766374	21.668694339394076
38-39	21.63981261446188	27.23756083571401	29.40572711422562	21.71689943559849
40-41	21.944356041872005	27.104648764231236	29.28209867438302	21.66889651951374
42-43	21.91572576489361	27.166501035034035	29.105326380358687	21.812446819713664
44-45	21.887903190912812	27.372656971564375	28.9332846633743	21.80615517414851
46-47	22.06698048991049	27.280057348448583	28.583822468094088	22.069139693546838
48-49	21.94236797431358	27.410344698292867	28.561945489056225	22.08534183833733
50-51	21.86506414674764	27.53522876359103	28.546831985778155	22.052875103883178
52-53	22.067718130937468	27.44008368896197	28.545230406924876	21.946967773175686
54-55	22.00326488384056	27.414568084030773	28.42165940158369	22.160507630544977
56-57	22.103259195427427	27.614487474704497	28.254658327114367	22.027595002753703
58-59	21.938350348277773	27.730690389665064	28.472630341233597	21.858328920823567
60-61	22.09695325568782	27.85776510146527	28.27124107787157	21.774040564975344
62-63	21.942457834339617	28.031280945658928	28.214283724283245	21.811977495718214
64-65	21.93024334569042	27.927288262201095	28.192179013851483	21.950289378257008
66-67	22.007478401976925	27.921698339247968	28.09619914243485	21.97462411634026
68-69	21.98999547763589	27.807870887841464	28.217073888519508	21.985059746003138
70-71	22.01351859374906	27.48500661471354	28.313003096553647	22.188471694983757
72-73	22.06013793692777	27.46891947521595	28.281667462025666	22.18927512583061
74-75	21.907162694451	27.61357740871024	28.280222827938385	22.199037068900378
76-77	22.072942694129434	27.54772457275466	28.201139221613104	22.1781935115028
78-79	22.028656565750858	27.60928969224386	28.132565587335023	22.22948815467026
80-81	22.058307033546438	27.627015790698806	28.08988301782611	22.224794157928642
82-83	22.09633009306643	27.601728538068244	28.158629326623196	22.14331204224213
84-85	22.22178632409923	27.576783745167983	28.106669952065104	22.094759978667685
86-87	22.056983660515655	27.644057712636734	28.157025946027854	22.14193268081976
88-89	22.126490310875223	27.64268291642858	28.07280320961376	22.15802356308244
90-91	22.16438934239209	27.67489801702189	28.088552466657156	22.072160173928868
92-93	22.205380090879974	27.613245702614293	28.059959310275516	22.121414896230217
94-95	22.065374373134723	27.73854413466864	28.042561507089925	22.15351998510671
96-97	22.13225070342105	27.764930692720636	28.029106617975465	22.07371198588285
98-99	22.345870991886926	28.345046068470815	28.08266304036296	21.226419899279296
100-101	22.965206479488256	30.855457239025764	25.465568483834456	20.713767797651524
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3355.0
1	3094.5
2	3391.0
3	4233.5
4	5222.5
5	7149.5
6	9392.5
7	13200.5
8	17308.0
9	19013.0
10	18125.0
11	16939.5
12	18170.5
13	22194.5
14	31325.0
15	42797.5
16	51139.0
17	54981.0
18	54302.0
19	50210.5
20	45001.5
21	40420.0
22	39185.0
23	43140.5
24	51592.5
25	64993.0
26	83420.0
27	104150.0
28	126617.0
29	150851.5
30	173442.5
31	198462.0
32	226312.0
33	254879.5
34	282779.0
35	305328.5
36	330391.5
37	352851.5
38	368997.0
39	383991.0
40	399322.5
41	415358.0
42	424949.0
43	432638.0
44	442074.0
45	454712.0
46	459577.5
47	454888.0
48	449518.0
49	438879.5
50	426468.0
51	412188.0
52	393172.0
53	371769.5
54	349772.0
55	325491.0
56	299217.0
57	271063.5
58	241729.0
59	210950.5
60	177328.5
61	143452.5
62	111969.5
63	85379.5
64	64405.0
65	48202.0
66	35622.5
67	26792.0
68	20409.0
69	15123.5
70	11062.0
71	7927.5
72	5642.0
73	3961.0
74	2677.0
75	1694.0
76	1022.5
77	622.5
78	380.5
79	223.5
80	144.0
81	96.5
82	74.0
83	56.0
84	39.0
85	31.5
86	24.5
87	15.5
88	9.5
89	5.0
90	3.5
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	1.5
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018072754337050295
4	0.0
5	0.057869324493383265
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.563826852790479E-6
14-15	3.651061482232383E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.565285731646844E-6
28-29	4.566767650077457E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.849935370195423E-5
40-41	0.0
42-43	0.0
44-45	9.41634614601429E-6
46-47	2.9422028918437673E-4
48-49	0.0
50-51	8.188304413606862E-5
52-53	2.424023938884733E-5
54-55	0.001839433552048651
56-57	0.002402370063344456
58-59	7.620087758670435E-4
60-61	0.002870828563986632
62-63	0.004678259011007009
64-65	0.0023609776004398803
66-67	0.007379692035462274
68-69	0.004154068220905122
70-71	0.0018319089160356225
72-73	7.592243533620732E-4
74-75	7.28176779371195E-4
76-77	0.0
78-79	0.0
80-81	1.7863974280640763E-4
82-83	0.0
84-85	0.0010744283517729115
86-87	0.0010505952205588477
88-89	1.907702010212939E-4
90-91	4.3060490356999797E-4
92-93	2.3465100556254516E-4
94-95	1.734466680114565E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	121.0
20-21	509.0
22-23	1088.0
24-25	1316.0
26-27	2774.0
28-29	6178.0
30-31	16555.0
32-33	21940.0
34-35	30459.0
36-37	48790.0
38-39	59025.0
40-41	58670.0
42-43	68636.0
44-45	82141.0
46-47	83139.0
48-49	76109.0
50-51	68834.0
52-53	64760.0
54-55	69319.0
56-57	72280.0
58-59	72716.0
60-61	71842.0
62-63	70108.0
64-65	78155.0
66-67	84133.0
68-69	84428.0
70-71	94009.0
72-73	73573.0
74-75	74812.0
76-77	78963.0
78-79	82605.0
80-81	84055.0
82-83	79597.0
84-85	79025.0
86-87	82587.0
88-89	85667.0
90-91	88831.0
92-93	87418.0
94-95	94176.0
96-97	157692.0
98-99	926864.0
100-101	7491819.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.5039767725402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90958692889598	74.24304081766672
2	7.652840816728465	12.780852836080825
3	1.8800520755710612	4.7097547454895565
4	0.6968640769774289	2.327636867901636
5	0.3158011874414662	1.3185327510426403
6	0.17506548387511542	0.8771198459508689
7	0.0968030801137884	0.5658409507332495
8	0.07015390129412137	0.46865037953339117
9	0.043027855021381387	0.3233697305649893
>10	0.15579822798300474	2.00781274058638
>50	0.0032190594255536065	0.1808682812708607
>100	6.919352570690042E-4	0.10617829008464302
>500	3.814942018648263E-5	0.02140733887464257
>1k	5.722199537758166E-5	0.06893442421957176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.7382961116742874E-5	0.0	0.0	0.0
5	0.0	2.7382961116742874E-5	0.0	0.0	0.0
6	0.0	2.7382961116742874E-5	0.0	0.0	0.0
7	0.0	2.7382961116742874E-5	0.0	9.127653705580958E-6	0.0
8	0.0	3.651061482232383E-5	0.0	9.127653705580958E-6	0.0
9	0.0	7.302122964464766E-5	0.0	1.8255307411161916E-5	1.8255307411161916E-5
10-11	0.0	7.302122964464766E-5	0.0	6.389357593906671E-5	1.8255307411161916E-5
12-13	0.0	7.758505649743814E-5	0.0	6.845740279185719E-5	2.2819134263952393E-5
14-15	0.0	1.6429776670045723E-4	0.0	8.214888335022862E-5	5.020209538069527E-5
16-17	0.0	1.734254204060382E-4	0.0	9.127653705580957E-5	6.845740279185719E-5
18-19	0.0	1.734254204060382E-4	0.0	9.127653705580957E-5	8.67127102030191E-5
20-21	0.0	1.8255307411161914E-4	0.0	9.127653705580957E-5	9.127653705580957E-5
22-23	0.0	2.0537220837557157E-4	0.0	1.0040419076139053E-4	9.127653705580957E-5
24-25	0.0	2.19063688933943E-4	0.0	1.4147863243650486E-4	9.127653705580957E-5
26-27	0.0	2.2819134263952394E-4	0.0	1.7798924725882868E-4	9.584036390860006E-5
28-29	0.0	2.327551694923144E-4	0.0	2.4644665005068585E-4	1.1409567131976197E-4
30-31	0.0	2.6470195746184776E-4	0.0	7.165208158881051E-4	1.1865949817255245E-4
32-33	0.0	2.6470195746184776E-4	0.0	0.0018529137022329344	1.3691480558371437E-4
34-35	0.0	2.9208491857859065E-4	0.0	0.003345285083095421	1.5517011299487628E-4
36-37	0.0	3.1946787969533354E-4	0.0	0.005709347392840888	1.6429776670045723E-4
38-39	0.0	3.422870139592859E-4	0.0	0.009574908737154424	1.6429776670045723E-4
40-41	0.0	4.0161676304556216E-4	0.0	0.013937927208422123	1.6429776670045723E-4
42-43	0.0	4.107444167511431E-4	0.0	0.01823248827689796	1.734254204060382E-4
44-45	0.0	4.335635510150955E-4	0.0	0.02266852797781031	1.8255307411161914E-4
46-47	0.0	4.746379926902098E-4	0.0	0.02762028011308798	2.19063688933943E-4
48-49	0.0	4.746379926902098E-4	0.0	0.032622234343746344	2.373189963451049E-4
50-51	0.0	5.157124343653241E-4	0.0	0.037505529076232155	2.4644665005068585E-4
52-53	0.0	5.202762612181146E-4	0.0	0.04276305761064679	2.601381306090573E-4
54-55	0.0	5.202762612181146E-4	0.0	0.047838033070949804	2.783934380202192E-4
56-57	0.0	5.202762612181146E-4	0.0	0.052844551128460954	2.9208491857859065E-4
58-59	0.0	5.33967741776486E-4	0.0	0.057586367228510266	3.0121257228417163E-4
60-61	0.0	5.750421834516004E-4	0.0	0.062378385423940266	3.1946787969533354E-4
62-63	0.0	5.978613177155527E-4	0.0	0.06707912708231445	3.2859553340091447E-4
64-65	0.0	6.937016816241528E-4	0.0	0.07164295393510495	3.2859553340091447E-4
66-67	0.0	7.165208158881051E-4	0.0	0.0762204722684538	3.422870139592859E-4
68-69	0.0	7.347761232992671E-4	0.0	0.08068389493048288	3.468508408120764E-4
70-71	0.0	7.484676038576385E-4	0.0	0.08523403030271498	3.605423213704478E-4
72-73	0.0	7.484676038576385E-4	0.0	0.08879381524789155	4.28999724162305E-4
74-75	0.0	7.986696992383338E-4	0.0	0.09178768566332211	4.335635510150955E-4
76-77	0.0	8.214888335022863E-4	0.0	0.09438906696941268	4.38127377867886E-4
78-79	0.0	8.351803140606577E-4	0.0	0.09689460791159465	4.38127377867886E-4
80-81	0.0	8.397441409134481E-4	0.0	0.09800818166367553	4.563826852790479E-4
82-83	0.0	8.397441409134481E-4	0.0	0.09837328781189877	4.837656463957908E-4
84-85	0.0	8.488717946190291E-4	0.0	0.09882967049717782	4.837656463957908E-4
86-87	0.0	8.534356214718196E-4	0.0	0.09902135122499502	4.883294732485812E-4
88-89	0.0	8.5799944832461E-4	0.0	0.09908980862778688	4.928933001013717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15910	0.0	53.54355	1
GTATCAA	29620	0.0	38.214428	1
TCAACGC	36755	0.0	30.9018	4
ATCAACG	37035	0.0	30.681067	3
TATCAAC	37910	0.0	30.180023	2
CAACGCA	37820	0.0	30.06621	5
AACGCAG	38860	0.0	29.32379	6
CTTATAC	8275	0.0	28.14805	1
ACGCAGA	44445	0.0	25.589895	7
CGCAGAG	44880	0.0	25.351578	8
TGGTATC	7055	0.0	24.79262	2
GTGGTAT	7520	0.0	24.419643	1
GCAGAGT	51355	0.0	22.189125	9
TATACAC	13385	0.0	21.116974	3
GAGTACT	32380	0.0	21.096455	12-13
CAGAGTA	50475	0.0	19.738867	10-11
GTACATG	33235	0.0	19.384737	1
AGAGTAC	47165	0.0	19.354153	10-11
TACATGG	32880	0.0	19.341974	2
GTACTTT	35655	0.0	19.122494	14-15
>>END_MODULE