FastQCFastQC Report
Fri 27 May 2016
SRR2139327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10259537
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165450.052.853271
CTTATAC95250.041.3403241
GTATCAA318600.039.8881
TCAACGC401100.031.9430984
ATCAACG401500.031.9118943
TATCAAC412750.031.2000452
CAACGCA412700.031.0557865
AACGCAG425050.030.1772756
TATACAC145500.029.9305973
GTGGTAT75150.028.2228931
TGGTATC72650.027.8779562
TTATACA148550.027.7654192
ACGCAGA477650.026.8449867
CGCAGAG484900.026.4615278
ACATCTC174200.023.5136578
ACACATC172900.023.4392266
GCAGAGT551850.023.2276239
TACACAT196800.022.1502195
GAGTACT312650.020.95074712-13
CACATCT192900.020.6712657
Produced by FastQC (version 0.11.5)