##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139327_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10259537
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.29147543402787	32.0	28.0	33.0	18.0	33.0
2	31.271643837338857	33.0	31.0	33.0	27.0	34.0
3	31.66297855351562	33.0	31.0	33.0	29.0	34.0
4	32.140345124736136	33.0	33.0	34.0	30.0	34.0
5	32.39715944296512	33.0	33.0	34.0	31.0	34.0
6	35.797257225155484	38.0	36.0	38.0	31.0	38.0
7	36.12926031652306	38.0	37.0	38.0	33.0	38.0
8	36.31640190000777	38.0	37.0	38.0	33.0	38.0
9	36.72300767568751	38.0	38.0	38.0	34.0	38.0
10-11	36.796721333526065	38.0	38.0	38.0	35.0	38.0
12-13	36.863487748033855	38.0	38.0	38.0	35.0	38.0
14-15	36.86288596649147	38.0	38.0	38.0	35.0	38.0
16-17	36.873884854647926	38.0	38.0	38.0	35.0	38.0
18-19	36.90082437443327	38.0	38.0	38.0	35.0	38.0
20-21	36.91592642840706	38.0	38.0	38.0	35.0	38.0
22-23	36.936354275508634	38.0	38.0	38.0	35.0	38.0
24-25	36.926123637831125	38.0	38.0	38.0	35.0	38.0
26-27	36.90873513651488	38.0	38.0	38.0	35.0	38.0
28-29	36.89009265786207	38.0	38.0	38.0	35.0	38.0
30-31	36.883212747690244	38.0	38.0	38.0	35.0	38.0
32-33	36.85467971573335	38.0	38.0	38.0	35.0	38.0
34-35	36.82696000575456	38.0	38.0	38.0	35.0	38.0
36-37	36.80173666642689	38.0	38.0	38.0	35.0	38.0
38-39	36.765001939627126	38.0	38.0	38.0	35.0	38.0
40-41	36.78161590744213	38.0	38.0	38.0	35.0	38.0
42-43	36.73792394530055	38.0	38.0	38.0	35.0	38.0
44-45	36.69269835363051	38.0	38.0	38.0	34.5	38.0
46-47	36.63723061650445	38.0	38.0	38.0	34.0	38.0
48-49	36.59443384158274	38.0	38.0	38.0	34.0	38.0
50-51	36.56064546427301	38.0	38.0	38.0	34.0	38.0
52-53	36.52937394265653	38.0	38.0	38.0	34.0	38.0
54-55	36.515869367737665	38.0	38.0	38.0	34.0	38.0
56-57	36.497851485362	38.0	38.0	38.0	34.0	38.0
58-59	36.48349519110894	38.0	38.0	38.0	34.0	38.0
60-61	36.480054273690115	38.0	38.0	38.0	34.0	38.0
62-63	36.46247373156601	38.0	38.0	38.0	34.0	38.0
64-65	36.45116727745402	38.0	38.0	38.0	34.0	38.0
66-67	36.42005725986228	38.0	38.0	38.0	34.0	38.0
68-69	36.40709307761513	38.0	38.0	38.0	34.0	38.0
70-71	36.40705470579171	38.0	38.0	38.0	34.0	38.0
72-73	36.386737666362436	38.0	38.0	38.0	34.0	38.0
74-75	36.37729888013602	38.0	38.0	38.0	34.0	38.0
76-77	36.366209902006254	38.0	38.0	38.0	34.0	38.0
78-79	36.353584420501235	38.0	38.0	38.0	34.0	38.0
80-81	36.35640923313204	38.0	38.0	38.0	34.0	38.0
82-83	36.33662542302784	38.0	38.0	38.0	33.5	38.0
84-85	36.326547711020964	38.0	38.0	38.0	33.0	38.0
86-87	36.31011134979918	38.0	38.0	38.0	33.0	38.0
88-89	36.31461016177701	38.0	37.5	38.0	33.0	38.0
90-91	36.322496815720534	38.0	38.0	38.0	33.5	38.0
92-93	36.2948166549284	38.0	37.0	38.0	33.0	38.0
94-95	36.28583000992113	38.0	37.0	38.0	33.0	38.0
96-97	36.281885079427994	38.0	37.0	38.0	33.0	38.0
98-99	36.31368126696118	38.0	37.0	38.0	33.0	38.0
100-101	35.760695507976635	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	7.0
19	25.0
20	81.0
21	470.0
22	1908.0
23	5780.0
24	13270.0
25	25546.0
26	42417.0
27	65257.0
28	94095.0
29	127476.0
30	164175.0
31	206339.0
32	262319.0
33	338900.0
34	467378.0
35	726853.0
36	1542453.0
37	6174785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78050861359533	20.2409328997985	15.99421104480641	23.984347441799763
2	14.17104884947537	21.5335350903262	42.04746276561993	22.247953294578497
3	17.740208340806195	26.444068938520825	31.177051866084227	24.638670854588753
4	12.252658185257289	18.870188781423565	37.95695653712248	30.92019649619666
5	13.250221560199273	37.480183443164975	35.39416746859414	13.875427528041618
6	29.289362668120404	38.31458476147608	18.63787810307619	13.758174467327327
7	26.176863536824325	33.0619110784434	22.462651092344615	18.29857429238766
8	24.298162772842478	36.900281172532445	20.872014009988952	17.929542044636126
9	25.336737905423995	17.15257715820899	20.93842051546771	36.5722644208993
10-11	24.05272284704466	27.131955369915815	28.48620264247792	20.329119140561605
12-13	25.16918648473123	24.521705999013406	28.69517893448798	21.613928581767382
14-15	22.124245604137577	25.882067353156586	26.581109824286365	25.412577218419475
16-17	21.402418062335563	29.235700402464555	27.2088594251378	22.153022110062082
18-19	21.44954007183755	27.83412155928674	29.494518124940726	21.221820243934985
20-21	22.384420705896435	26.797622198647968	29.449696395259352	21.368260700196245
22-23	21.961838382669622	27.011733551711465	29.351225601281104	21.675202464337808
24-25	21.745465932140906	27.205547772974235	29.53326235827146	21.515723936613398
26-27	21.595976018944985	27.262023130334523	29.5899356596923	21.552065191028188
28-29	21.350084602492647	27.108964938064243	29.81359165440729	21.727358805035816
30-31	21.78394368481122	26.941943669483663	29.4626965179069	21.81141612779821
32-33	21.732556045899823	27.09718508616657	29.671712540962947	21.49854632697066
34-35	21.781421247144863	27.393573116097595	29.49423090119499	21.33077473556255
36-37	21.549532498109457	27.18928062467884	29.51609305026688	21.74509382694482
38-39	21.451124856545594	27.1677384388206	29.69647084365823	21.684665860975578
40-41	21.737818574269216	27.062428893334182	29.636830752165093	21.562921780231505
42-43	21.85971500938884	27.017650015127153	29.4678041620931	21.65483081339091
44-45	21.65101347347888	27.378462024729	29.260043213882835	21.710481287909282
46-47	21.952961143176157	27.14829082308986	28.814429123603304	22.084318910130683
48-49	21.807688267845528	27.25463494456125	28.83982396536965	22.097852822223572
50-51	21.644842738657314	27.44800472849052	28.888316430189718	22.018836102662448
52-53	21.875317420998666	27.42235074627834	28.95298367304463	21.749348159678366
54-55	21.80768683476281	27.453532779207123	28.682675995388852	22.056104390641217
56-57	22.00496160557161	27.72619271407904	28.347398018366338	21.92144766198302
58-59	21.682521225862246	27.99379149323328	28.723704232458786	21.59998304844569
60-61	21.981404931771685	28.23378127188875	28.238718081789436	21.546095714550127
62-63	21.683427981192484	28.478356544305978	28.26742395707899	21.570791517422546
64-65	21.661024289354895	28.419882801870344	28.24779711083483	21.671295797939923
66-67	21.758480748918153	28.33300369167686	28.15759315938273	21.750922400022255
68-69	21.781620612248954	28.169874914744696	28.275900505682582	21.772603967323768
70-71	21.868804417258442	27.671765325114624	28.45770052760781	22.001729730019125
72-73	21.904463366728294	27.58607445259651	28.471013110110555	22.03844907056464
74-75	21.769017302908328	27.70754142928699	28.461384707113407	22.062056560691275
76-77	21.92577447449234	27.639666274604924	28.37903827407404	22.055520976828692
78-79	21.910056832649115	27.714194764638613	28.29556116348519	22.08018723922709
80-81	21.925157381690763	27.721032544285862	28.256438556242287	22.09737151778109
82-83	21.970671360176492	27.679004441377998	28.342752488776117	22.007571709669392
84-85	22.07729191477096	27.680124902338566	28.26052894571162	21.982054237178854
86-87	21.906861102721038	27.759648444384815	28.288910587490847	22.044579865403303
88-89	21.97577671760101	27.746797846443872	28.22661097319158	22.050814462763537
90-91	22.028792170138598	27.767121346702034	28.246578473061234	21.95750801009813
92-93	22.068220878354765	27.715378263774358	28.19564041232767	22.0207604455432
94-95	21.93990863409483	27.84245560782236	28.19130848970717	22.02632726837564
96-97	21.980954502983273	27.873917915978634	28.203530456965414	21.941597124072672
98-99	22.23978664992519	28.39058706941336	28.247702965018657	21.121923315642796
100-101	22.82613723187166	30.93724985263981	25.624889606329305	20.611723309159228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3533.0
1	3255.5
2	3559.5
3	4398.0
4	5468.5
5	7525.0
6	9672.5
7	13650.0
8	18089.0
9	19758.5
10	18781.0
11	17280.0
12	18256.5
13	22676.0
14	32122.5
15	43724.5
16	52348.0
17	56169.0
18	54834.5
19	49630.0
20	43360.5
21	37977.0
22	35945.0
23	39437.0
24	47745.5
25	60836.5
26	78287.5
27	98000.5
28	119174.5
29	141299.5
30	162449.0
31	185812.5
32	212389.5
33	238587.0
34	264542.0
35	289347.5
36	315101.5
37	336009.5
38	350928.0
39	364806.0
40	380565.0
41	394859.0
42	402407.5
43	410072.0
44	420515.0
45	440757.0
46	448657.0
47	437349.5
48	429164.5
49	415796.0
50	398211.0
51	382428.0
52	362988.5
53	342407.0
54	321932.5
55	298180.5
56	272669.5
57	246007.5
58	218802.0
59	189790.0
60	158258.5
61	127205.0
62	98515.0
63	74573.5
64	56050.5
65	41597.5
66	30430.0
67	22601.5
68	16806.0
69	12246.0
70	9062.0
71	6471.5
72	4509.5
73	3080.5
74	2040.0
75	1359.5
76	871.5
77	521.5
78	327.5
79	208.5
80	140.5
81	92.0
82	67.5
83	47.5
84	30.5
85	23.0
86	20.5
87	19.5
88	18.0
89	12.5
90	9.0
91	8.0
92	5.5
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018811765092323369
4	0.0
5	0.059612826582720053
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.9241085635735807E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.750377181153282E-6
28-29	4.876926849413569E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	2.9659758085126474E-5
40-41	0.0
42-43	0.0
44-45	1.0072460779222689E-5
46-47	2.4374352270630872E-4
48-49	0.0
50-51	6.70312479052735E-5
52-53	2.0761307814987058E-5
54-55	0.001802176684670847
56-57	0.0024404537182153257
58-59	7.310304356046149E-4
60-61	0.002796557885546065
62-63	0.004822896467091232
64-65	0.00237820940442594
66-67	0.0075195271354104535
68-69	0.004436389785179413
70-71	0.001979758834144003
72-73	7.490212741752952E-4
74-75	7.945225163708527E-4
76-77	0.0
78-79	0.0
80-81	1.979401993548896E-4
82-83	0.0
84-85	9.718640613440596E-4
86-87	9.984286407207148E-4
88-89	1.0861905475112418E-4
90-91	3.7174639631481094E-4
92-93	2.3394632606380013E-4
94-95	2.487940485975635E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	138.0
20-21	495.0
22-23	1060.0
24-25	1334.0
26-27	2834.0
28-29	6540.0
30-31	17408.0
32-33	22122.0
34-35	30150.0
36-37	48183.0
38-39	58179.0
40-41	57013.0
42-43	66718.0
44-45	80231.0
46-47	80313.0
48-49	73104.0
50-51	65579.0
52-53	60711.0
54-55	64282.0
56-57	67405.0
58-59	68898.0
60-61	69913.0
62-63	69138.0
64-65	79501.0
66-67	86604.0
68-69	84648.0
70-71	101572.0
72-73	68339.0
74-75	69049.0
76-77	73472.0
78-79	76225.0
80-81	78588.0
82-83	74416.0
84-85	73679.0
86-87	75862.0
88-89	80793.0
90-91	83198.0
92-93	82078.0
94-95	88695.0
96-97	146247.0
98-99	863559.0
100-101	6961264.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.14711831731059
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.55618495953699	73.63191588560262
2	7.843504573651569	13.043296056155473
3	1.953720006121467	4.873385655236351
4	0.7469790921077784	2.484366358081708
5	0.33560226995802744	1.3952180823879057
6	0.19831537594193382	0.9893611216551541
7	0.11105488870054728	0.6463725779350115
8	0.061481064695368334	0.4089578688400014
9	0.044341535863981096	0.3318183835968336
>10	0.1449694235515047	1.80087222721984
>50	0.002959244851399311	0.16273193529848867
>100	7.661960829907714E-4	0.12000833915244258
>500	6.068488348450786E-5	0.0350008060438585
>1k	6.0684052971259686E-5	0.07669470279429914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9494057090490535E-5	0.0	0.0	0.0
3	0.0	1.9494057090490535E-5	0.0	9.747028545245267E-6	0.0
4	0.0	2.9241085635735804E-5	0.0	9.747028545245267E-6	0.0
5	0.0	2.9241085635735804E-5	0.0	9.747028545245267E-6	0.0
6	0.0	2.9241085635735804E-5	0.0	9.747028545245267E-6	9.747028545245267E-6
7	9.747028545245267E-6	2.9241085635735804E-5	0.0	1.9494057090490535E-5	9.747028545245267E-6
8	1.9494057090490535E-5	3.898811418098107E-5	0.0	1.9494057090490535E-5	9.747028545245267E-6
9	2.9241085635735804E-5	1.0721731399769795E-4	0.0	3.898811418098107E-5	9.747028545245267E-6
10-11	2.9241085635735804E-5	1.2183785681556584E-4	0.0	4.3861628453603704E-5	9.747028545245267E-6
12-13	2.9241085635735804E-5	1.2671137108818847E-4	0.0	5.848217127147161E-5	9.747028545245267E-6
14-15	2.9241085635735804E-5	1.7057299954179218E-4	0.0	6.822919981671687E-5	1.9494057090490535E-5
16-17	3.898811418098107E-5	1.7544651381441482E-4	0.0	8.284974263458477E-5	2.9241085635735804E-5
18-19	3.898811418098107E-5	1.9006705663228272E-4	0.0	9.747028545245268E-5	2.9241085635735804E-5
20-21	3.898811418098107E-5	2.0468759945015062E-4	0.0	1.0721731399769795E-4	3.898811418098107E-5
22-23	3.898811418098107E-5	2.4854922790375434E-4	0.0	1.315848853608111E-4	5.360865699884897E-5
24-25	3.898811418098107E-5	2.729167992668675E-4	0.0	1.3645839963343374E-4	5.848217127147161E-5
26-27	3.898811418098107E-5	2.8266382781211275E-4	0.0	1.7057299954179218E-4	6.822919981671687E-5
28-29	3.898811418098107E-5	2.8753734208473536E-4	0.0	3.8013411326456544E-4	6.822919981671687E-5
30-31	3.898811418098107E-5	3.4114599908358436E-4	0.0	0.001077046654249602	7.797622836196214E-5
32-33	3.898811418098107E-5	3.4601951335620697E-4	0.0	0.0024367571363113167	9.747028545245268E-5
34-35	3.898811418098107E-5	4.483633130812823E-4	0.0	0.004239957417181692	1.169643425429432E-4
36-37	3.898811418098107E-5	5.360865699884897E-4	0.0	0.0067157026676739895	1.46205428178679E-4
38-39	3.898811418098107E-5	5.945687412599614E-4	0.0	0.010653502199953077	1.46205428178679E-4
40-41	4.873514272622634E-5	6.627979410766782E-4	0.0	0.014810609874500186	1.46205428178679E-4
42-43	4.873514272622634E-5	6.774184838945461E-4	0.0	0.01887512077786746	1.46205428178679E-4
44-45	6.335568554409424E-5	7.553947122565083E-4	0.0	0.023524453393949454	1.46205428178679E-4
46-47	9.259677117983004E-5	8.138768835279799E-4	0.0	0.028305370895392257	1.5595245672392428E-4
48-49	1.0721731399769795E-4	8.187503978006025E-4	0.0	0.0331155294824708	1.7544651381441482E-4
50-51	1.3645839963343374E-4	9.45461768888791E-4	0.0	0.038534877353627164	1.7544651381441482E-4
52-53	1.3645839963343374E-4	9.552087974340363E-4	0.0	0.043471747311793894	1.9494057090490535E-4
54-55	1.46205428178679E-4	9.600823117066589E-4	0.0	0.048350135098689156	2.1930814226801853E-4
56-57	1.46205428178679E-4	9.747028545245268E-4	0.0	0.05360378348457635	2.2418165654064116E-4
58-59	1.46205428178679E-4	9.795763687971495E-4	0.0	0.058803823213464695	2.3392868508588643E-4
60-61	1.46205428178679E-4	9.844498830697721E-4	0.0	0.06413057431344124	2.4854922790375434E-4
62-63	1.46205428178679E-4	0.0010039439401602626	0.0	0.069286752413876	2.5342274217637694E-4
64-65	1.5107894245130164E-4	0.0011111612541579606	0.0	0.07391171745859487	2.5342274217637694E-4
66-67	1.608259709965469E-4	0.0011355288255210736	0.0	0.07898504581639504	2.5342274217637694E-4
68-69	1.6569948526916955E-4	0.001145275854066319	0.0	0.08418995905955601	2.5342274217637694E-4
70-71	1.7544651381441482E-4	0.0011550228826115643	0.0	0.08875156841873079	2.5342274217637694E-4
72-73	1.7544651381441482E-4	0.0011598963968841869	0.0	0.09208992569547729	3.021578849026033E-4
74-75	1.7544651381441482E-4	0.0011940109967925453	0.0	0.0952382159155915	3.021578849026033E-4
76-77	1.7544651381441482E-4	0.0012329991109735264	0.0	0.09789428119417085	3.216519419930938E-4
78-79	1.7544651381441482E-4	0.0012476196537913942	0.0	0.10015559181666775	3.2652545626571643E-4
80-81	1.7544651381441482E-4	0.0012476196537913942	0.0	0.10106693898564818	3.4114599908358436E-4
82-83	1.7544651381441482E-4	0.0012671137108818849	0.0	0.10146169364173062	3.8013411326456544E-4
84-85	1.8519354235966009E-4	0.0012963547965176205	0.0	0.10177847206945109	3.8013411326456544E-4
86-87	1.8519354235966009E-4	0.0013548369677890922	0.0	0.10194417155472027	3.8013411326456544E-4
88-89	1.99814085177528E-4	0.0013743310248795827	0.0	0.10199290669744648	3.8500762753718805E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16545	0.0	52.85327	1
CTTATAC	9525	0.0	41.340324	1
GTATCAA	31860	0.0	39.888	1
TCAACGC	40110	0.0	31.943098	4
ATCAACG	40150	0.0	31.911894	3
TATCAAC	41275	0.0	31.200045	2
CAACGCA	41270	0.0	31.055786	5
AACGCAG	42505	0.0	30.177275	6
TATACAC	14550	0.0	29.930597	3
GTGGTAT	7515	0.0	28.222893	1
TGGTATC	7265	0.0	27.877956	2
TTATACA	14855	0.0	27.765419	2
ACGCAGA	47765	0.0	26.844986	7
CGCAGAG	48490	0.0	26.461527	8
ACATCTC	17420	0.0	23.513657	8
ACACATC	17290	0.0	23.439226	6
GCAGAGT	55185	0.0	23.227623	9
TACACAT	19680	0.0	22.150219	5
GAGTACT	31265	0.0	20.950747	12-13
CACATCT	19290	0.0	20.671265	7
>>END_MODULE