FastQCFastQC Report
Fri 27 May 2016
SRR2139326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9849412
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141900.053.6576271
GTATCAA265850.039.8273741
TCAACGC341850.031.3043144
ATCAACG345800.030.9596563
TATCAAC350550.030.7018682
CAACGCA350850.030.4888675
AACGCAG364400.029.4205446
ACGCAGA415400.025.7770147
CGCAGAG423800.025.2352498
TGGTATC63000.023.3270032
GTGGTAT66850.023.157761
GCAGAGT482450.022.1403759
TACATGG324900.020.951872
GTACATG329300.020.6984041
GAGTACT287250.020.57281112-13
ACATGGG330000.020.1919863
AGAGTAC437500.019.76321210-11
CAGAGTA469500.019.48367110-11
GTACTTT314000.018.72336814-15
AGTACTT310650.018.1954412-13
Produced by FastQC (version 0.11.5)