##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9849412
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.226392803956216	32.0	28.0	33.0	18.0	33.0
2	31.22471229754629	33.0	31.0	33.0	27.0	34.0
3	31.613539265085063	33.0	31.0	33.0	29.0	34.0
4	32.110030629239596	33.0	33.0	34.0	29.0	34.0
5	32.374089945673916	33.0	33.0	34.0	31.0	34.0
6	35.750410278298844	38.0	36.0	38.0	31.0	38.0
7	36.099405121848896	38.0	37.0	38.0	33.0	38.0
8	36.28786713359133	38.0	37.0	38.0	33.0	38.0
9	36.70387501304646	38.0	38.0	38.0	34.0	38.0
10-11	36.783962941138014	38.0	38.0	38.0	34.5	38.0
12-13	36.855167343999824	38.0	38.0	38.0	35.0	38.0
14-15	36.85406844591332	38.0	38.0	38.0	35.0	38.0
16-17	36.86897857455857	38.0	38.0	38.0	35.0	38.0
18-19	36.893422977940205	38.0	38.0	38.0	35.0	38.0
20-21	36.910528473857894	38.0	38.0	38.0	35.0	38.0
22-23	36.93412611340967	38.0	38.0	38.0	35.0	38.0
24-25	36.9274071788179	38.0	38.0	38.0	35.0	38.0
26-27	36.907453660170106	38.0	38.0	38.0	35.0	38.0
28-29	36.89071243978091	38.0	38.0	38.0	35.0	38.0
30-31	36.88567861058547	38.0	38.0	38.0	35.0	38.0
32-33	36.861725404564424	38.0	38.0	38.0	35.0	38.0
34-35	36.839639635982124	38.0	38.0	38.0	35.0	38.0
36-37	36.81379676008902	38.0	38.0	38.0	35.0	38.0
38-39	36.797616195248075	38.0	38.0	38.0	35.0	38.0
40-41	36.78382072187407	38.0	38.0	38.0	35.0	38.0
42-43	36.73688184361168	38.0	38.0	38.0	35.0	38.0
44-45	36.69781885796465	38.0	38.0	38.0	34.5	38.0
46-47	36.647019878907386	38.0	38.0	38.0	34.0	38.0
48-49	36.61007833595494	38.0	38.0	38.0	34.0	38.0
50-51	36.578758579417794	38.0	38.0	38.0	34.0	38.0
52-53	36.5518139760095	38.0	38.0	38.0	34.0	38.0
54-55	36.53864878893162	38.0	38.0	38.0	34.0	38.0
56-57	36.52095721155388	38.0	38.0	38.0	34.0	38.0
58-59	36.511115178491096	38.0	38.0	38.0	34.0	38.0
60-61	36.508315408006325	38.0	38.0	38.0	34.0	38.0
62-63	36.49545563690894	38.0	38.0	38.0	34.0	38.0
64-65	36.48490590955053	38.0	38.0	38.0	34.0	38.0
66-67	36.4604905635933	38.0	38.0	38.0	34.0	38.0
68-69	36.44888499297262	38.0	38.0	38.0	34.0	38.0
70-71	36.452937843657466	38.0	38.0	38.0	34.0	38.0
72-73	36.434519046314804	38.0	38.0	38.0	34.0	38.0
74-75	36.42418961737131	38.0	38.0	38.0	34.0	38.0
76-77	36.4115193712216	38.0	38.0	38.0	34.0	38.0
78-79	36.39932104855396	38.0	38.0	38.0	34.0	38.0
80-81	36.395222861284275	38.0	38.0	38.0	34.0	38.0
82-83	36.373143001726284	38.0	38.0	38.0	34.0	38.0
84-85	36.361581808712785	38.0	38.0	38.0	34.0	38.0
86-87	36.34713635496459	38.0	38.0	38.0	33.5	38.0
88-89	36.3466370354618	38.0	38.0	38.0	34.0	38.0
90-91	36.35223488108238	38.0	38.0	38.0	34.0	38.0
92-93	36.32058770558679	38.0	37.0	38.0	33.0	38.0
94-95	36.30951014482858	38.0	37.0	38.0	33.0	38.0
96-97	36.30518954264093	38.0	37.0	38.0	33.0	38.0
98-99	36.33713965750446	38.0	37.0	38.0	34.0	38.0
100-101	35.80841839574647	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	10.0
19	14.0
20	61.0
21	399.0
22	1782.0
23	5418.0
24	12176.0
25	23251.0
26	39245.0
27	60678.0
28	87519.0
29	118979.0
30	153377.0
31	195558.0
32	249565.0
33	324911.0
34	448721.0
35	697778.0
36	1486370.0
37	5943597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99131115644264	19.92912876423486	16.074756543842415	24.004803535480086
2	14.419114562371846	21.418060286238408	41.43969203440774	22.72313311698201
3	17.880514517378515	26.017851141662852	30.94131216348892	25.160322177469713
4	12.635292340294018	18.362111362586926	37.7534212194596	31.24917507765946
5	13.69668452509509	37.067752245645394	35.10701952250156	14.128543706757954
6	30.083054704179297	37.8038607786942	18.389351567382906	13.7237329497436
7	26.6427579636226	32.7455080567246	22.488641961570906	18.12309201808189
8	24.692265893639135	36.67466646739927	20.539642366468172	18.093425272493423
9	25.526640575092195	17.084634087801383	20.889054087695794	36.499671249410625
10-11	24.47887752081038	26.798543913078266	28.290551760856385	20.432026805254974
12-13	25.429573866947592	24.24884856070596	28.524946463809208	21.79663110853724
14-15	22.444605559582307	25.54772575694643	26.478050611970726	25.529618071500533
16-17	21.803301557493988	28.706652742315985	27.050386358089195	22.43965934210083
18-19	21.816408938929552	27.53425280615736	29.235933068897918	21.413405186015165
20-21	22.74029820196115	26.63374429942805	29.160520247722893	21.465437250887913
22-23	22.38926018715707	26.578407993155594	29.065546808255284	21.96678501143205
24-25	22.114470889267864	26.760532487163974	29.181185323559657	21.94381130000851
26-27	22.07623410998041	26.885026262067363	29.124206911362215	21.91453271659001
28-29	21.967671245986768	26.938152789071157	29.12851631928547	21.9656596456566
30-31	22.396964215445532	26.751117727147477	29.02180444935224	21.830113608054752
32-33	22.01721035065402	26.98682895877277	29.004945760609296	21.991014929963914
34-35	22.189354095287754	26.950919342395665	29.054621801562824	21.805104760753764
36-37	22.134186279728578	26.88484532479022	29.030125382526194	21.950843012955012
38-39	21.976590395612035	26.95721083280032	29.110618485882046	21.955580285705597
40-41	22.320605899817103	26.796126418134747	28.94932787715488	21.933939804893274
42-43	22.180647917932774	26.869830633636266	28.825641269194097	22.123880179236867
44-45	22.206648253385765	27.051245410362206	28.635207598770013	22.106898737482013
46-47	22.340598381464225	26.963340863583664	28.31847975795654	22.377580996995572
48-49	22.213429882971155	27.120007362512787	28.313649428421105	22.35291332609495
50-51	22.179205140047213	27.247779464885596	28.274578047429276	22.29843734763791
52-53	22.442894780664705	27.164949710046514	28.13946585744867	22.25268965184011
54-55	22.35384126233569	27.119688004799723	28.128823397425563	22.39764733543902
56-57	22.366991723074413	27.30233350232157	28.018839236662885	22.31183553794114
58-59	22.290236736160594	27.380292082342862	28.134575378347172	22.19489580314938
60-61	22.404416521720762	27.37998481623805	28.083954192749488	22.131644469291697
62-63	22.341131527319373	27.498114709444078	27.969088272258407	22.191665490978142
64-65	22.272013786806273	27.468864818330385	27.97215570054693	22.286965694316407
66-67	22.377445014133354	27.49265831633254	27.815476361595902	22.314420307938203
68-69	22.31925251703459	27.360377872259058	27.957093218882314	22.363276391824037
70-71	22.342466621948564	27.07536634699974	28.062589496538287	22.519577534513406
72-73	22.42377923975715	27.11853222655517	27.958525437061986	22.499163096625697
74-75	22.214975250428132	27.288977643277732	27.978270814272644	22.517776292021487
76-77	22.3986809822009	27.20823075810529	27.881629221608495	22.511459038085317
78-79	22.35753906986692	27.285684583179805	27.80367173426896	22.55310461268432
80-81	22.400846269760642	27.291435412737368	27.760432374705484	22.547285942796503
82-83	22.4270293405906	27.291970766123214	27.829621338439075	22.451378554847107
84-85	22.544601115018683	27.215184831981286	27.77884596767098	22.46136808532905
86-87	22.330821527069077	27.322303904901347	27.849694198941684	22.497180369087893
88-89	22.41644931386826	27.33020037436998	27.768991444532055	22.48435886722971
90-91	22.468263506187977	27.32796837975154	27.79329641506139	22.410471698999093
92-93	22.538959338035394	27.303894233787478	27.712668506928743	22.444477921248378
94-95	22.41081386455638	27.398336165231463	27.714484198806133	22.476365771406027
96-97	22.442161784576065	27.391286940371018	27.752825629763162	22.41372564528976
98-99	22.672049428288474	27.99202677763759	27.758951070071475	21.576972724002463
100-101	23.296208482757883	30.517406795630613	25.08236727841603	21.104017443195477
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3051.0
1	2888.0
2	3285.0
3	4084.5
4	5029.5
5	6940.0
6	8993.5
7	12598.5
8	16507.5
9	17963.0
10	16954.5
11	15589.5
12	16593.0
13	20262.5
14	28645.5
15	39069.5
16	46252.5
17	49341.0
18	48348.5
19	44078.5
20	38936.0
21	34539.0
22	32681.0
23	35285.5
24	42152.0
25	53587.0
26	69401.0
27	87407.0
28	106268.5
29	126093.0
30	144589.5
31	164856.0
32	188407.0
33	212710.5
34	237117.5
35	257320.0
36	280127.0
37	301759.0
38	316085.0
39	328250.0
40	343767.5
41	358853.0
42	368084.0
43	378873.0
44	394359.0
45	403391.0
46	402931.0
47	402036.0
48	400252.0
49	396339.0
50	390875.5
51	383317.0
52	370662.5
53	355934.0
54	340726.0
55	320472.5
56	295640.5
57	268713.0
58	241820.0
59	211801.0
60	177949.5
61	144436.0
62	113057.0
63	86436.0
64	64646.5
65	47775.0
66	35332.0
67	26808.5
68	20348.5
69	14659.0
70	10572.0
71	7720.5
72	5412.5
73	3624.5
74	2423.0
75	1523.5
76	903.0
77	531.0
78	331.5
79	205.0
80	125.0
81	75.0
82	49.5
83	37.5
84	28.5
85	21.5
86	15.5
87	11.5
88	6.5
89	4.0
90	3.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0018478260428135202
4	0.0
5	0.05781055762516585
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.0458671035387696E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	2.5722458988883063E-5
40-41	0.0
42-43	0.0
44-45	5.2372944807086355E-6
46-47	2.586559340290122E-4
48-49	0.0
50-51	6.429341112550331E-5
52-53	4.314140155196878E-5
54-55	0.0017530806049636932
56-57	0.002218877752843033
58-59	8.091803547964111E-4
60-61	0.0025839786117547183
62-63	0.004484729440405737
64-65	0.002019423024403799
66-67	0.0068435100731705605
68-69	0.003968224017338509
70-71	0.0017133889171312814
72-73	6.982631925047032E-4
74-75	6.392621062020799E-4
76-77	0.0
78-79	0.0
80-81	1.8657767433216026E-4
82-83	0.0
84-85	8.884483274470057E-4
86-87	9.458440016397103E-4
88-89	1.310511626235095E-4
90-91	3.5292582123160147E-4
92-93	1.9101267534645243E-4
94-95	1.9298889361783156E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	91.0
20-21	485.0
22-23	919.0
24-25	1238.0
26-27	2550.0
28-29	5811.0
30-31	15282.0
32-33	20144.0
34-35	27091.0
36-37	43336.0
38-39	53194.0
40-41	52879.0
42-43	61566.0
44-45	73644.0
46-47	74359.0
48-49	68872.0
50-51	61702.0
52-53	58647.0
54-55	62448.0
56-57	65507.0
58-59	65812.0
60-61	65335.0
62-63	63320.0
64-65	68954.0
66-67	73634.0
68-69	74108.0
70-71	78829.0
72-73	67458.0
74-75	68532.0
76-77	71948.0
78-79	74882.0
80-81	76117.0
82-83	72686.0
84-85	71652.0
86-87	74861.0
88-89	77931.0
90-91	81047.0
92-93	79675.0
94-95	85817.0
96-97	142752.0
98-99	837793.0
100-101	6726504.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.23353162006464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.31233229645412	72.62234970342115
2	7.824634611882457	12.868946755433763
3	2.0515097269079123	5.061086699896559
4	0.7891677879685958	2.59584216981808
5	0.368538468395351	1.5153109896999644
6	0.20748047305909337	1.0237111225110551
7	0.13261378766504003	0.7633710070846725
8	0.07780279517289647	0.5118398893583835
9	0.05712922111139994	0.4228143849624574
>10	0.174319545423159	2.182088331040379
>50	0.003619949159198689	0.19725707722829836
>100	7.39518141169692E-4	0.11001432561313529
>500	2.2364219784360468E-5	0.010298897138853578
>1k	8.945443980609965E-5	0.11506864679308362
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0152890345129232E-5
2	0.0	5.076445172564616E-5	0.0	0.0	1.0152890345129232E-5
3	0.0	5.076445172564616E-5	0.0	0.0	1.0152890345129232E-5
4	0.0	1.0152890345129231E-4	0.0	0.0	1.0152890345129232E-5
5	0.0	1.2183468414155078E-4	0.0	0.0	1.0152890345129232E-5
6	9.137601310616309E-5	1.2183468414155078E-4	0.0	0.0	1.0152890345129232E-5
7	9.137601310616309E-5	1.2183468414155078E-4	0.0	0.0	2.0305780690258465E-5
8	9.137601310616309E-5	1.2183468414155078E-4	0.0	0.0	3.0458671035387696E-5
9	9.137601310616309E-5	1.8275202621232617E-4	0.0	2.0305780690258465E-5	3.0458671035387696E-5
10-11	9.137601310616309E-5	1.8275202621232617E-4	0.0	4.5688006553081544E-5	3.0458671035387696E-5
12-13	9.137601310616309E-5	1.8275202621232617E-4	0.0	5.584089689821078E-5	3.0458671035387696E-5
14-15	9.137601310616309E-5	2.538222586282308E-4	0.0	6.599378724334E-5	3.0458671035387696E-5
16-17	2.2844003276540774E-4	2.9443382000874773E-4	0.0	7.107023241590462E-5	3.0458671035387696E-5
18-19	2.3351647793797234E-4	3.2489249104413544E-4	0.0	8.122312276103385E-5	5.076445172564616E-5
20-21	2.3351647793797234E-4	3.5535116207952315E-4	0.0	1.2183468414155078E-4	5.076445172564616E-5
22-23	2.3351647793797234E-4	3.756569427697816E-4	0.0	1.3198757448668E-4	6.091734207077539E-5
24-25	2.3351647793797234E-4	3.858098331149108E-4	0.0	1.7767558103976157E-4	7.107023241590462E-5
26-27	2.3351647793797234E-4	3.9596272346004006E-4	0.0	2.233635875928431E-4	7.107023241590462E-5
28-29	2.3351647793797234E-4	3.9596272346004006E-4	0.0	4.924151817387678E-4	7.107023241590462E-5
30-31	2.3351647793797234E-4	4.568800655308154E-4	0.0	0.001309722854521671	7.107023241590462E-5
32-33	2.3859292311053694E-4	4.670329558759447E-4	0.0	0.0032590778007864835	7.614667758846925E-5
34-35	2.538222586282308E-4	5.736383044998017E-4	0.0	0.005680542148099805	8.122312276103386E-5
36-37	2.538222586282308E-4	6.599378724334001E-4	0.0	0.009366041343381717	8.122312276103386E-5
38-39	2.538222586282308E-4	6.954729886413524E-4	0.0	0.014960283923547923	8.122312276103386E-5
40-41	2.538222586282308E-4	7.513138855395632E-4	0.0	0.02122461726649266	8.122312276103386E-5
42-43	2.842809296636185E-4	8.122312276103386E-4	0.0	0.0276463204097869	8.122312276103386E-5
44-45	2.893573748361831E-4	9.239130214067601E-4	0.0	0.03436753381826245	8.122312276103386E-5
46-47	2.9443382000874773E-4	9.949832538226648E-4	0.0	0.041951742906073994	8.629956793359847E-5
48-49	2.9443382000874773E-4	0.001005136144167794	0.0	0.049749162691133234	1.1168179379642156E-4
50-51	3.096631555264416E-4	0.0011625059445172971	0.0	0.05764811137964378	1.1168179379642156E-4
52-53	3.147396006990062E-4	0.0011828117252075557	0.0	0.06498357465399965	1.2183468414155078E-4
54-55	3.2489249104413544E-4	0.0012031175058978142	0.0	0.07209567434076267	1.3198757448668E-4
56-57	3.2489249104413544E-4	0.001228499731760637	0.0	0.07815187343163227	1.370640196592446E-4
58-59	3.2489249104413544E-4	0.0012386526221057662	0.0	0.08338061195937382	1.4721691000437387E-4
60-61	3.502747169069585E-4	0.0012589584027960247	0.0	0.08881240829401796	1.6244624552206772E-4
62-63	3.6550405242465235E-4	0.001309722854521671	0.0	0.0938583947955472	1.8275202621232617E-4
64-65	3.6550405242465235E-4	0.0014518633193534801	0.0	0.09861502392224022	1.8275202621232617E-4
66-67	3.858098331149108E-4	0.0014518633193534801	0.0	0.10343764683617662	1.8782847138489077E-4
68-69	3.858098331149108E-4	0.0014975513259065617	0.0	0.10844302177632534	1.929049165574554E-4
70-71	4.1119205897773394E-4	0.0015381628872870785	0.0	0.11354992561992533	2.1321069724771388E-4
72-73	4.6195651070338005E-4	0.0015432393324596432	0.0	0.11750955285452573	2.4366936828310157E-4
74-75	4.721094010485093E-4	0.0015432393324596432	0.0	0.12077878354565734	2.4366936828310157E-4
76-77	4.8733873656620313E-4	0.001588927339012725	0.0	0.12345914659677146	2.4366936828310157E-4
78-79	4.974916269113324E-4	0.0016193860100481125	0.0	0.12602782785408917	2.4366936828310157E-4
80-81	4.974916269113324E-4	0.0016295389003932416	0.0	0.12702281110791183	2.7920448449105385E-4
82-83	4.974916269113324E-4	0.0016447682359109355	0.0	0.12743400316688958	3.451982717343939E-4
84-85	4.974916269113324E-4	0.0016599975714286295	0.0	0.1277741249934514	3.604276072520878E-4
86-87	5.02568072083897E-4	0.0016752269069463233	0.0	0.12790611256793807	3.70580497597217E-4
88-89	5.076445172564616E-4	0.0017462971393622279	0.0	0.1279873356906991	3.858098331149108E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14190	0.0	53.657627	1
GTATCAA	26585	0.0	39.827374	1
TCAACGC	34185	0.0	31.304314	4
ATCAACG	34580	0.0	30.959656	3
TATCAAC	35055	0.0	30.701868	2
CAACGCA	35085	0.0	30.488867	5
AACGCAG	36440	0.0	29.420544	6
ACGCAGA	41540	0.0	25.777014	7
CGCAGAG	42380	0.0	25.235249	8
TGGTATC	6300	0.0	23.327003	2
GTGGTAT	6685	0.0	23.15776	1
GCAGAGT	48245	0.0	22.140375	9
TACATGG	32490	0.0	20.95187	2
GTACATG	32930	0.0	20.698404	1
GAGTACT	28725	0.0	20.572811	12-13
ACATGGG	33000	0.0	20.191986	3
AGAGTAC	43750	0.0	19.763212	10-11
CAGAGTA	46950	0.0	19.483671	10-11
GTACTTT	31400	0.0	18.723368	14-15
AGTACTT	31065	0.0	18.19544	12-13
>>END_MODULE