FastQCFastQC Report
Fri 27 May 2016
SRR2139325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10963539
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA233200.050.1886141
CTTATAC135200.047.9414941
GTATCAA429150.037.3871651
TATACAC193050.036.032443
TTATACA190200.035.0273552
TATCAAC554950.029.3734192
ATCAACG551500.029.366873
TCAACGC551650.029.3203014
CAACGCA558700.028.950325
AACGCAG576150.028.1004146
ACATCTC245050.026.8622118
ACACATC247750.026.4509246
TACACAT262350.026.3059375
GTGGTAT101750.025.827411
ACGCAGA644500.025.1073117
CGCAGAG648100.024.9613728
CACATCT261750.024.9560247
TGGTATC107850.023.821672
ATACACA306900.023.7183914
CATCTCC284050.022.9672559
Produced by FastQC (version 0.11.5)