##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10963539
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.77798117925243	32.0	30.0	33.0	18.0	34.0
2	31.454014073375394	33.0	31.0	33.0	27.0	34.0
3	31.778890283511554	33.0	31.0	33.0	29.0	34.0
4	32.17920363123623	33.0	33.0	34.0	30.0	34.0
5	32.4321366485767	33.0	33.0	34.0	31.0	34.0
6	35.881307121724106	38.0	36.0	38.0	31.0	38.0
7	36.21400534991484	38.0	37.0	38.0	33.0	38.0
8	36.41313293089029	38.0	37.0	38.0	34.0	38.0
9	36.76269533040381	38.0	38.0	38.0	34.0	38.0
10-11	36.821528842101074	38.0	38.0	38.0	35.0	38.0
12-13	36.88027556612879	38.0	38.0	38.0	35.0	38.0
14-15	36.87551560677625	38.0	38.0	38.0	35.0	38.0
16-17	36.89341475412273	38.0	38.0	38.0	35.0	38.0
18-19	36.92069002536498	38.0	38.0	38.0	35.0	38.0
20-21	36.93876302962843	38.0	38.0	38.0	35.0	38.0
22-23	36.95900996181048	38.0	38.0	38.0	35.0	38.0
24-25	36.951049604883096	38.0	38.0	38.0	35.0	38.0
26-27	36.92606644994109	38.0	38.0	38.0	35.0	38.0
28-29	36.898421227299394	38.0	38.0	38.0	35.0	38.0
30-31	36.891671940111195	38.0	38.0	38.0	35.0	38.0
32-33	36.857175354876475	38.0	38.0	38.0	35.0	38.0
34-35	36.8216953520904	38.0	38.0	38.0	35.0	38.0
36-37	36.79186710149345	38.0	38.0	38.0	35.0	38.0
38-39	36.734362754834	38.0	38.0	38.0	35.0	38.0
40-41	36.77651444280184	38.0	38.0	38.0	35.0	38.0
42-43	36.73862363547467	38.0	38.0	38.0	35.0	38.0
44-45	36.69356003390571	38.0	38.0	38.0	34.5	38.0
46-47	36.64161606392379	38.0	38.0	38.0	34.0	38.0
48-49	36.604684756492205	38.0	38.0	38.0	34.0	38.0
50-51	36.57493148525944	38.0	38.0	38.0	34.0	38.0
52-53	36.559499801540156	38.0	38.0	38.0	34.0	38.0
54-55	36.547454183821465	38.0	38.0	38.0	34.0	38.0
56-57	36.53488507507112	38.0	38.0	38.0	34.0	38.0
58-59	36.518708771177614	38.0	38.0	38.0	34.0	38.0
60-61	36.51840615534694	38.0	38.0	38.0	34.0	38.0
62-63	36.501108229889326	38.0	38.0	38.0	34.0	38.0
64-65	36.494184112630805	38.0	38.0	38.0	34.0	38.0
66-67	36.465432608777704	38.0	38.0	38.0	34.0	38.0
68-69	36.45012187045768	38.0	38.0	38.0	34.0	38.0
70-71	36.4562682548005	38.0	38.0	38.0	34.0	38.0
72-73	36.43885495610831	38.0	38.0	38.0	34.0	38.0
74-75	36.43565281189919	38.0	38.0	38.0	34.0	38.0
76-77	36.43374256353633	38.0	38.0	38.0	34.0	38.0
78-79	36.429000014923076	38.0	38.0	38.0	34.0	38.0
80-81	36.434331178736784	38.0	38.0	38.0	34.0	38.0
82-83	36.41927529591295	38.0	38.0	38.0	34.0	38.0
84-85	36.416264926307406	38.0	38.0	38.0	34.0	38.0
86-87	36.40478738494134	38.0	38.0	38.0	34.0	38.0
88-89	36.41294847953763	38.0	38.0	38.0	34.0	38.0
90-91	36.42266709822478	38.0	38.0	38.0	34.0	38.0
92-93	36.39793651974804	38.0	38.0	38.0	34.0	38.0
94-95	36.39092244393693	38.0	38.0	38.0	34.0	38.0
96-97	36.38905850077476	38.0	38.0	38.0	34.0	38.0
98-99	36.41949278695609	38.0	38.0	38.0	34.0	38.0
100-101	35.881281657683886	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	12.0
18	22.0
19	30.0
20	111.0
21	489.0
22	2030.0
23	6289.0
24	14311.0
25	27541.0
26	46192.0
27	70422.0
28	100780.0
29	134396.0
30	172183.0
31	215836.0
32	269891.0
33	351882.0
34	485202.0
35	754657.0
36	1616591.0
37	6694672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.52005825856049	20.845103027407482	16.131415230063944	23.503423483968085
2	13.898878820059837	21.6716062213123	42.625241721673994	21.80427323695387
3	17.04954565861395	27.48089952862913	32.06891657671538	23.40063823604153
4	11.999063441102367	19.350485276697608	38.40313789187962	30.247313390320407
5	12.5858376482223	38.0245015479571	35.74300571815955	13.646655085661049
6	28.25897732474888	38.91630248225504	18.84928762509989	13.975432567896187
7	25.049028420476272	33.90752748724659	22.946139927992228	18.097304164284907
8	24.065477397398777	36.72902518064651	21.30804660794293	17.89745081401179
9	25.20156128418023	16.946772388003545	21.249881083106466	36.60178524470976
10-11	23.764912953746048	27.220042725255052	28.686854673477242	20.328189647521665
12-13	25.105539602930204	24.780056183503163	28.87303674812653	21.241367465440103
14-15	22.076325936509157	26.009957190810916	27.01664901116738	24.897067861512536
16-17	21.014441595911684	29.480056576621838	27.444573326186006	22.060928501280472
18-19	21.10248342255179	27.74947031246024	30.12412324159197	21.023923023396005
20-21	22.09701582212908	26.583401331197916	30.09835944715934	21.221223399513665
22-23	21.761027736872357	26.95395431459061	29.93177666464728	21.353241283889744
24-25	21.61181238724273	27.240302851942843	30.067842918610594	21.08004184220383
26-27	21.462188235574626	27.279289301738036	30.130223700328866	21.128298762358476
28-29	20.79568034659917	27.116774253594546	30.32234845291869	21.765196946887595
30-31	21.674191311490535	26.763563304885363	30.54794874923622	21.014296634387883
32-33	21.094822425751207	27.350891541983202	30.13579577264906	21.418490259616533
34-35	21.109622734554883	27.512285096340783	30.580130048571817	20.797962120532514
36-37	21.582620120811885	27.212446685591	29.99625131072161	21.2086818828755
38-39	21.225408307414607	27.045600042243684	30.19747056609709	21.531521084244616
40-41	21.40776812320859	26.939847560820677	30.208404500413216	21.443979815557515
42-43	21.704808053802623	26.927019508548106	29.907298352870292	21.46087408477898
44-45	21.395330617999896	27.39561627602524	29.639422188321706	21.569630917653154
46-47	21.841869305092338	27.109630692918575	28.93878829024765	22.109711711741433
48-49	21.720042498143552	27.24656733687197	28.905439275694732	22.127950889289746
50-51	21.451927566581844	27.503994828889095	28.993117757707658	22.050959846821403
52-53	21.743809954961787	27.579814196307524	28.928808361647768	21.747567487082918
54-55	21.63462888739469	27.744267983085173	28.495838421034602	22.125264708485535
56-57	22.017510132689242	28.0582153363008	27.955125337818014	21.969149193191946
58-59	21.47515228856647	28.54788845906532	28.515649972839864	21.46130927952835
60-61	21.907755906873767	28.95488794351308	27.789398157197926	21.34795799241523
62-63	21.508390829092924	29.290792156935417	27.648672620787423	21.552144393184236
64-65	21.417361914775398	29.173067240402066	27.753396624029275	21.65617422079326
66-67	21.63462493855908	29.011396681428288	27.67210175903462	21.681876620978013
68-69	21.629806853190704	28.629620087849045	27.92459047699846	21.815982581961787
70-71	21.668883022577013	28.048956293402327	28.165382181584814	22.11677850243585
72-73	21.870155620796105	27.910198340252368	28.199381211811424	22.020264827140103
74-75	21.761499997672587	28.008517646380298	28.114653305884254	22.115329050062858
76-77	21.8410665323956	27.95935423894508	28.03567347827257	22.163905750386746
78-79	21.865100633484026	27.945327631008105	28.068691561915248	22.12088017359262
80-81	21.873039140790322	27.975089138905428	28.03063764161492	22.121234078689326
82-83	21.952359996253033	27.935236869797098	28.11018657339186	22.002216560558015
84-85	22.084308540715394	27.96786251155624	27.93811403945713	22.009714908271235
86-87	21.81260769511886	28.10837736029014	27.975232008315366	22.103782936275636
88-89	21.972854784289776	27.974285476089232	27.977056225399593	22.075803514221395
90-91	22.106639556313905	27.938847536010332	27.9658647546226	21.988648153053166
92-93	22.081145925638463	27.99301209357828	27.889015157739856	22.036826823043405
94-95	21.893978453165	28.136674644841015	27.92042491624198	22.04892198575201
96-97	21.884493523678945	28.138830171792335	27.938850597892213	22.037825706636507
98-99	22.11095192896684	28.723844609765887	27.935990226000275	21.229213235267
100-101	22.756403477863167	31.149593777242035	25.354151587992263	20.739851156902535
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7909.0
1	7398.0
2	6229.5
3	5957.5
4	8208.5
5	12678.5
6	15965.0
7	16899.5
8	16085.0
9	14427.0
10	13281.5
11	13667.5
12	16831.5
13	26819.5
14	45895.0
15	68191.0
16	86341.5
17	95332.0
18	95321.0
19	86243.5
20	74145.5
21	63760.5
22	57164.0
23	58151.0
24	65970.5
25	80743.0
26	101721.0
27	124493.0
28	148596.5
29	175450.0
30	198802.5
31	222972.0
32	251490.5
33	280533.0
34	307038.5
35	331160.5
36	358310.0
37	382161.0
38	395214.0
39	401984.0
40	412427.5
41	422760.5
42	428979.5
43	444214.5
44	491227.5
45	519035.5
46	500297.0
47	482874.5
48	464872.5
49	445590.5
50	430225.5
51	409465.5
52	386685.5
53	366399.0
54	342695.0
55	315107.5
56	289007.0
57	257589.5
58	224439.5
59	196486.5
60	167654.5
61	136735.5
62	104713.0
63	78972.0
64	62171.5
65	47429.0
66	33442.5
67	23979.5
68	17661.5
69	12501.5
70	8700.0
71	6270.0
72	4610.0
73	3316.5
74	2171.0
75	1372.0
76	899.0
77	513.0
78	305.5
79	202.5
80	148.5
81	106.0
82	73.0
83	49.0
84	33.0
85	25.5
86	21.0
87	17.5
88	13.5
89	9.0
90	6.5
91	3.5
92	2.5
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0017968650451282199
4	0.0
5	0.05944248476700817
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.5605711805284775E-6
14-15	4.560571180528477E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.827053241290654E-5
28-29	1.8285172626657735E-5
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	3.731772855196205E-5
40-41	0.0
42-43	0.0
44-45	1.438358430290678E-5
46-47	3.104903331260487E-4
48-49	0.0
50-51	6.938688705792313E-5
52-53	2.508365272766412E-5
54-55	0.001968496167100124
56-57	0.0024409677732506393
58-59	7.733464795360664E-4
60-61	0.0027872156720917343
62-63	0.004385166723459752
64-65	0.0021756645378489562
66-67	0.0070382496287821434
68-69	0.004116202020793153
70-71	0.0015851316864775797
72-73	7.922601687997382E-4
74-75	6.073940518865517E-4
76-77	0.0
78-79	0.0
80-81	1.8202964581787184E-4
82-83	0.0
84-85	9.980188124141275E-4
86-87	0.001082718079798269
88-89	1.416158753120244E-4
90-91	4.802206470430632E-4
92-93	2.5913840525340544E-4
94-95	4.483317798637161E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1736.0
20-21	5441.0
22-23	3764.0
24-25	4565.0
26-27	7285.0
28-29	13596.0
30-31	28610.0
32-33	35985.0
34-35	47301.0
36-37	73187.0
38-39	93431.0
40-41	89373.0
42-43	101326.0
44-45	120904.0
46-47	118156.0
48-49	106473.0
50-51	124705.0
52-53	85354.0
54-55	88098.0
56-57	92169.0
58-59	97460.0
60-61	101158.0
62-63	98207.0
64-65	114011.0
66-67	124121.0
68-69	119530.0
70-71	146014.0
72-73	91056.0
74-75	92303.0
76-77	96195.0
78-79	100178.0
80-81	104651.0
82-83	97403.0
84-85	95751.0
86-87	98230.0
88-89	102454.0
90-91	106794.0
92-93	103477.0
94-95	106228.0
96-97	157586.0
98-99	836417.0
100-101	6732856.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.84536596547261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.77891985336794	67.55336774073845
2	8.1034909314207	12.616384343086656
3	2.4572694869392246	5.738611274597192
4	1.0447150035075898	3.253048871106733
5	0.5387626425883305	2.0970087540406857
6	0.3256970508341314	1.5212403669634855
7	0.20806289588800098	1.1337712591966225
8	0.12867936968245874	0.8013674100109873
9	0.08755084266895144	0.6133884649335034
>10	0.3156985254468537	3.760469615289655
>50	0.00804982287568871	0.4211584446248951
>100	0.002917948935137525	0.39053971203570964
>500	1.5910799458549536E-4	0.07481031339709926
>1k	2.6517850397045256E-5	0.024833429978370958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.824228472211391E-5	0.0	0.0	9.121142361056955E-6
3	0.0	1.824228472211391E-5	0.0	0.0	9.121142361056955E-6
4	0.0	4.560571180528477E-5	0.0	0.0	2.7363427083170863E-5
5	0.0	6.384799652739869E-5	0.0	0.0	2.7363427083170863E-5
6	0.0	6.384799652739869E-5	0.0	0.0	4.560571180528477E-5
7	0.0	8.209028124951259E-5	0.0	9.121142361056955E-6	7.296913888845564E-5
8	0.0	8.209028124951259E-5	0.0	9.121142361056955E-6	8.209028124951259E-5
9	0.0	1.185748506937404E-4	0.0	9.121142361056955E-6	9.121142361056955E-5
10-11	0.0	1.6874113367955368E-4	0.0	9.121142361056955E-6	9.121142361056955E-5
12-13	0.0	1.778622760406106E-4	0.0	9.121142361056955E-6	1.003325659716265E-4
14-15	0.0	2.4627084374853777E-4	0.0	9.121142361056955E-6	1.4593827777691128E-4
16-17	0.0	2.8731598437329404E-4	0.0	9.121142361056955E-6	1.7330170486008214E-4
18-19	0.0	3.283611249980503E-4	0.0	1.3681713541585433E-5	2.052257031237815E-4
20-21	0.0	3.92209121525449E-4	0.0	1.824228472211391E-5	2.3258913020695232E-4
22-23	0.0	4.332542621502053E-4	0.0	1.824228472211391E-5	2.8731598437329404E-4
24-25	0.0	5.655108263855312E-4	0.0	5.016628298581325E-5	3.557245520812212E-4
26-27	0.0	6.11116538190816E-4	0.0	1.3681713541585433E-4	3.6940626562280665E-4
28-29	0.0	7.342519600650848E-4	0.0	4.8798111631654707E-4	3.8764855034492055E-4
30-31	0.0	9.303565208278093E-4	0.0	0.00158707877082391	4.10451406247563E-4
32-33	0.0	9.394776631888663E-4	0.0	0.0037807135086581076	4.332542621502053E-4
34-35	0.0	0.0011127793680489485	0.0	0.006507935074614137	4.606176892333762E-4
36-37	0.0	0.001158385079854233	0.0	0.01069453941833928	4.6517826041390464E-4
38-39	0.0	0.001185748506937404	0.0	0.017695016180450492	4.742994027749616E-4
40-41	0.0	0.001290641644089559	0.0	0.025192595201239308	4.7885997395549014E-4
42-43	0.0	0.0013499290694364292	0.0	0.03179174169946401	5.016628298581325E-4
44-45	0.0	0.0014730644913106981	0.0	0.03913882187129539	5.472685416634173E-4
46-47	0.0	0.0015733970572823245	0.0	0.04696476201708226	5.700713975660596E-4
48-49	0.0	0.0016281239114486665	0.0	0.055014170150715025	6.01995395829759E-4
50-51	0.0	0.0017649410468645209	0.0	0.0634329845499706	6.065559670102874E-4
52-53	0.0	0.0018105467586698055	0.0	0.07123156126867428	6.749645347182146E-4
54-55	0.0	0.0018196679010308624	0.0	0.07856495972696408	7.388125312456133E-4
56-57	0.0	0.0018698341840166757	0.0	0.08563384505678322	7.889788142314265E-4
58-59	0.0	0.0020659387447794003	0.0	0.09245189897167329	8.482662395782967E-4
60-61	0.0	0.0021252261701262703	0.0	0.09909209061052275	8.665085243004107E-4
62-63	0.0	0.0021480290260289126	0.0	0.10530814912958306	8.801902378419961E-4
64-65	0.0	0.0022802855902642385	0.0	0.11145579908093545	8.89311380203053E-4
66-67	0.0	0.002330451873250052	0.0	0.11781779587777268	8.9843252256411E-4
68-69	0.0	0.0024262238680411497	0.0	0.12395632468676401	9.257959496472808E-4
70-71	0.0	0.0024444661527632636	0.0	0.12998083921624212	9.85083374994151E-4
72-73	0.0	0.002458147866304849	0.0	0.1347147121016307	0.0010534919427020782
74-75	0.0	0.0025174352916517194	0.0	0.13852278903737197	0.001071734227424192
76-77	0.0	0.002590404430540175	0.0	0.14176991571790826	0.0010762947986047205
78-79	0.0	0.002599525572901232	0.0	0.14471604470052962	0.0010762947986047205
80-81	0.0	0.002608646715262289	0.0	0.146056852627605	0.0011127793680489485
82-83	0.0	0.002677055282970216	0.0	0.14657219717100473	0.001158385079854233
84-85	0.0	0.0028731598437329404	0.0	0.14713314742620975	0.0011629456510347616
86-87	0.0	0.0030419009774124944	0.0	0.1474478268376662	0.001185748506937404
88-89	0.0	0.0031239912586620068	0.0	0.1475435988324573	0.001231354218742689
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23320	0.0	50.188614	1
CTTATAC	13520	0.0	47.941494	1
GTATCAA	42915	0.0	37.387165	1
TATACAC	19305	0.0	36.03244	3
TTATACA	19020	0.0	35.027355	2
TATCAAC	55495	0.0	29.373419	2
ATCAACG	55150	0.0	29.36687	3
TCAACGC	55165	0.0	29.320301	4
CAACGCA	55870	0.0	28.95032	5
AACGCAG	57615	0.0	28.100414	6
ACATCTC	24505	0.0	26.862211	8
ACACATC	24775	0.0	26.450924	6
TACACAT	26235	0.0	26.305937	5
GTGGTAT	10175	0.0	25.82741	1
ACGCAGA	64450	0.0	25.107311	7
CGCAGAG	64810	0.0	24.961372	8
CACATCT	26175	0.0	24.956024	7
TGGTATC	10785	0.0	23.82167	2
ATACACA	30690	0.0	23.718391	4
CATCTCC	28405	0.0	22.967255	9
>>END_MODULE