FastQCFastQC Report
Fri 27 May 2016
SRR2139324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11198288
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152700.050.2193451
GTATCAA271250.037.3028341
TCAACGC328900.031.0159284
ATCAACG331250.030.8097653
CAACGCA340250.030.0069735
TATCAAC349800.029.488022
AACGCAG350150.029.190986
ACGCAGA401400.025.4421847
CGCAGAG409550.024.9146868
GTGGTAT73300.024.6054671
TGGTATC69550.024.5507722
GCAGAGT475100.021.4496259
GAGTACT290750.020.15173512-13
GTACATG323800.019.758151
TACATGG320950.019.4845852
CAGAGTA463000.019.35210-11
ACATGGG323900.018.8487153
AGAGTAC432000.018.8009810-11
TATACCG7550.018.5088275
GTACTTT321750.018.17006714-15
Produced by FastQC (version 0.11.5)