FastQCFastQC Report
Fri 27 May 2016
SRR2139323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11455853
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164750.051.426611
CTTATAC115450.042.3125651
GTATCAA315550.040.6062161
TCAACGC380200.033.5398144
ATCAACG380600.033.4601253
TATCAAC390600.032.6815152
CAACGCA391450.032.5647775
TATACAC162950.032.4899943
AACGCAG402700.031.6801596
TTATACA174100.029.433922
ACGCAGA456600.027.9023117
CGCAGAG462700.027.5157748
GTGGTAT78550.026.9931261
TGGTATC74550.025.7549922
ACACATC203400.024.108936
GCAGAGT533300.023.8894639
TACACAT221350.023.7203525
ACATCTC210950.023.638078
CACATCT229650.021.2963337
GAGTACT320950.021.16276612-13
Produced by FastQC (version 0.11.5)