##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11455853
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.597798871895442	32.0	25.0	33.0	18.0	33.0
2	30.750043143884614	33.0	30.0	33.0	27.0	33.0
3	30.545493469582755	33.0	29.0	33.0	25.0	33.0
4	31.813020208970908	33.0	32.0	33.0	29.0	34.0
5	31.930829506977787	33.0	32.0	33.0	30.0	34.0
6	35.1136063809478	37.0	35.0	38.0	29.0	38.0
7	36.15715093411202	38.0	37.0	38.0	33.0	38.0
8	36.24008818898078	38.0	37.0	38.0	33.0	38.0
9	36.63601453335688	38.0	38.0	38.0	34.0	38.0
10-11	36.77062895272836	38.0	38.0	38.0	34.5	38.0
12-13	36.83826289495859	38.0	38.0	38.0	35.0	38.0
14-15	36.878935946541915	38.0	38.0	38.0	35.0	38.0
16-17	36.885733519799885	38.0	38.0	38.0	35.0	38.0
18-19	36.90537221453523	38.0	38.0	38.0	35.0	38.0
20-21	36.92047539555276	38.0	38.0	38.0	35.0	38.0
22-23	36.93530063137712	38.0	38.0	38.0	35.0	38.0
24-25	36.92805838499843	38.0	38.0	38.0	35.0	38.0
26-27	36.9047349582476	38.0	38.0	38.0	35.0	38.0
28-29	36.88313043935737	38.0	38.0	38.0	35.0	38.0
30-31	36.87416947412615	38.0	38.0	38.0	35.0	38.0
32-33	36.843421872062734	38.0	38.0	38.0	35.0	38.0
34-35	36.817695196351096	38.0	38.0	38.0	35.0	38.0
36-37	36.78701588165042	38.0	38.0	38.0	35.0	38.0
38-39	36.74268378627726	38.0	38.0	38.0	35.0	38.0
40-41	36.76800652634553	38.0	38.0	38.0	35.0	38.0
42-43	36.72193659887965	38.0	38.0	38.0	35.0	38.0
44-45	36.682645329195736	38.0	38.0	38.0	34.0	38.0
46-47	36.63841748672145	38.0	38.0	38.0	34.0	38.0
48-49	36.601211696227	38.0	38.0	38.0	34.0	38.0
50-51	36.57057559068518	38.0	38.0	38.0	34.0	38.0
52-53	36.545049994017276	38.0	38.0	38.0	34.0	38.0
54-55	36.53138187115033	38.0	38.0	38.0	34.0	38.0
56-57	36.51480900948692	38.0	38.0	38.0	34.0	38.0
58-59	36.499519452860476	38.0	38.0	38.0	34.0	38.0
60-61	36.498360441240166	38.0	38.0	38.0	34.0	38.0
62-63	36.481395202132205	38.0	38.0	38.0	34.0	38.0
64-65	36.47134792687886	38.0	38.0	38.0	34.0	38.0
66-67	36.438610948118836	38.0	38.0	38.0	34.0	38.0
68-69	36.42250929306333	38.0	38.0	38.0	34.0	38.0
70-71	36.42277912743418	38.0	38.0	38.0	34.0	38.0
72-73	36.40153753422959	38.0	38.0	38.0	34.0	38.0
74-75	36.3925897383858	38.0	38.0	38.0	34.0	38.0
76-77	36.38133526138316	38.0	38.0	38.0	34.0	38.0
78-79	36.37259254936126	38.0	38.0	38.0	34.0	38.0
80-81	36.369514355982446	38.0	38.0	38.0	34.0	38.0
82-83	36.35252107456984	38.0	38.0	38.0	34.0	38.0
84-85	36.34072491632345	38.0	38.0	38.0	34.0	38.0
86-87	36.32525527672397	38.0	38.0	38.0	33.5	38.0
88-89	36.328992767145124	38.0	38.0	38.0	33.5	38.0
90-91	36.33644118727355	38.0	38.0	38.0	34.0	38.0
92-93	36.306935211538914	38.0	37.0	38.0	33.0	38.0
94-95	36.2994729473464	38.0	37.0	38.0	33.0	38.0
96-97	36.29364067759198	38.0	37.0	38.0	33.0	38.0
98-99	36.324686170613674	38.0	37.0	38.0	33.0	38.0
100-101	35.78445100394401	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	12.0
18	51.0
19	124.0
20	224.0
21	656.0
22	2369.0
23	6701.0
24	15690.0
25	29592.0
26	48852.0
27	74167.0
28	104987.0
29	141838.0
30	184223.0
31	232813.0
32	296363.0
33	385650.0
34	535152.0
35	841443.0
36	1829558.0
37	6725388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.37015427834139	20.397320042427218	14.461376206555723	25.77114947267567
2	13.75022008400422	20.310194273617164	43.60911404851302	22.330471593865596
3	17.69043289706965	25.88955105838973	30.086368163720657	26.333647880819967
4	12.128498855563178	18.671721782742846	38.81629766024407	30.383481701449906
5	13.548805064143817	36.85011540227283	35.51623737580193	14.084842157781424
6	29.37921776754642	38.14434420553406	18.69022760679628	13.786210420123233
7	26.174977978505837	33.035130600925136	22.498324655527615	18.291566765041416
8	23.987231679736116	37.03530413667145	20.90737372415655	18.07009045943589
9	24.992717696360106	17.535464185862022	21.117519577110496	36.35429854066738
10-11	23.92473524232547	27.082588262960428	28.560592563469523	20.43208393124458
12-13	24.966080657634137	24.29844813825736	28.80325018137017	21.932221022738332
14-15	22.07594469532792	25.75480325575157	26.53098314533549	25.638268903585022
16-17	21.568891465349633	29.230481571298096	27.110512853124074	22.090114110228196
18-19	21.533678024674373	27.88213588285394	29.220176795215515	21.36400929725617
20-21	22.300186537637455	26.89855011484779	29.248414552292644	21.55284879522211
22-23	21.938032219697917	27.13245369474745	29.12719286912031	21.802321216434322
24-25	21.844666657557465	27.28660141014812	29.24460254681937	21.624129385475047
26-27	21.778847705071012	27.408710639665486	29.226798229476703	21.585643425786806
28-29	21.369365256791525	27.282571329378168	29.40736170344164	21.940701710388662
30-31	21.957869669817505	26.986830703788513	29.501726489133762	21.553573137260223
32-33	21.567644078700702	27.42941471353491	29.2478292320927	21.75511197567169
34-35	21.635169077309012	27.477399514928408	29.505545217558137	21.381886190204447
36-37	21.871238971189218	27.29946965380121	29.207470924029295	21.621820450980277
38-39	21.576320917733966	27.212967136703135	29.409035602741817	21.801676342821082
40-41	21.80402105790122	27.0828778419935	29.35166398780301	21.761437112302268
42-43	21.90921285401493	27.10963152860512	29.21204075054014	21.76911486683981
44-45	21.75087537508676	27.382392048782133	29.065880160668733	21.800852415462373
46-47	22.085165928544765	27.150740821167275	28.594840070360032	22.169253179927924
48-49	21.90084302326512	27.26841789951868	28.636488992009077	22.194250085207116
50-51	21.702759822817093	27.490363283927504	28.719316364359997	22.08756052889541
52-53	21.931151140126577	27.472222966575764	28.798470898985702	21.798154994311954
54-55	21.866738403538285	27.4477466453005	28.523281848038106	22.162233103123103
56-57	22.055254756815227	27.715234979692095	28.225844938411154	22.003665325081528
58-59	21.738337389095665	28.023605350389673	28.596738398883044	21.641318861631618
60-61	22.011199048112843	28.294546470292442	28.08354977350216	21.610704708092562
62-63	21.73617460836208	28.541513989157323	28.04429630117636	21.678015101304236
64-65	21.721332969504147	28.444354454778743	28.063451422997876	21.770861152719238
66-67	21.8325056243682	28.37881970291114	27.95911710414627	21.82955756857439
68-69	21.882591317603957	28.14349889830632	28.098080679809446	21.875829104280278
70-71	21.913825887645828	27.739129841273684	28.26056685400516	22.08647741707533
72-73	22.00630914064638	27.59171547050655	28.27058431727178	22.131391071575294
74-75	21.871564113247537	27.699451657444136	28.278052071299438	22.15093215800889
76-77	22.00371330580806	27.67031711304527	28.16561875398301	22.160350827163654
78-79	21.993100420066085	27.714939758949203	28.086695075852752	22.205264745131952
80-81	22.017038503690202	27.718798386373354	28.07957368639325	22.18458942354319
82-83	22.052631399454395	27.697795355448072	28.184003439748135	22.065569805349398
84-85	22.147154843254583	27.677963237565535	28.126957991346114	22.047923927833775
86-87	22.018334908405546	27.73648774914172	28.122893007213296	22.122284335239435
88-89	22.07393419891864	27.719337386958447	28.06238120566516	22.144347208457752
90-91	22.11950027194626	27.744325237494472	28.057354592561158	22.078819897998113
92-93	22.117220745499612	27.693564396931308	28.095396524942345	22.093818332626736
94-95	22.028164252452495	27.82636538671704	28.041089101873233	22.104381258957233
96-97	22.0641117462612	27.8433688751809	28.074328798358323	22.01819058019958
98-99	22.28652995034594	28.421801006860324	28.097673151636084	21.193995891157652
100-101	22.878795224281802	30.900183012981103	25.52562400928139	20.6953977534557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2971.0
1	2820.0
2	3298.0
3	4216.5
4	5389.0
5	7630.5
6	9668.5
7	12337.0
8	15865.0
9	17660.0
10	17120.0
11	16377.5
12	17809.5
13	22233.0
14	31078.0
15	41932.0
16	50801.0
17	56410.0
18	56450.5
19	51697.5
20	46031.0
21	41332.5
22	39909.5
23	43679.0
24	52651.0
25	67097.0
26	86343.0
27	108250.5
28	132298.0
29	157681.0
30	182395.5
31	208578.0
32	236376.0
33	265083.5
34	293737.5
35	317792.0
36	344476.5
37	369881.0
38	388046.5
39	404247.0
40	422375.5
41	440754.5
42	453511.0
43	467499.0
44	502467.0
45	522343.0
46	509177.0
47	497323.0
48	480605.5
49	462587.5
50	450253.5
51	433544.5
52	410206.5
53	384795.0
54	358800.5
55	331098.0
56	301422.0
57	269495.5
58	238714.0
59	208288.5
60	174225.0
61	140093.5
62	109396.0
63	83915.0
64	63254.5
65	46469.0
66	34230.5
67	26323.5
68	20113.5
69	14778.5
70	10584.5
71	7382.5
72	5060.0
73	3374.0
74	2154.5
75	1299.0
76	812.5
77	463.5
78	284.0
79	184.0
80	117.5
81	81.0
82	58.0
83	43.5
84	31.5
85	20.5
86	14.0
87	11.5
88	6.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.002121186436313385
4	0.0
5	0.06289361429480633
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.055206801274423E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.32720741579798E-5
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.6780753280857884E-4
48-49	0.0
50-51	8.291622632776286E-5
52-53	2.3180554778363065E-5
54-55	0.0018894955802598939
56-57	0.00235794264525279
58-59	7.426521546443976E-4
60-61	0.0026783738610536845
62-63	0.0046239279677490865
64-65	0.0021722839882495405
66-67	0.007350075660390389
68-69	0.004184314056377546
70-71	0.0016888726013151686
72-73	7.748378161306844E-4
74-75	7.300770342810713E-4
76-77	0.0
78-79	0.0
80-81	2.3400911699519815E-4
82-83	0.0
84-85	0.0010214064565801173
86-87	0.0010411385891611064
88-89	1.7244852438492192E-4
90-91	5.170054240399576E-4
92-93	2.4188481928675204E-4
94-95	2.7765750580151474E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	153.0
20-21	565.0
22-23	1246.0
24-25	1480.0
26-27	2947.0
28-29	6857.0
30-31	18067.0
32-33	23632.0
34-35	32241.0
36-37	51208.0
38-39	62144.0
40-41	61738.0
42-43	70905.0
44-45	85137.0
46-47	86604.0
48-49	78611.0
50-51	70799.0
52-53	66868.0
54-55	71182.0
56-57	74546.0
58-59	76683.0
60-61	78903.0
62-63	78457.0
64-65	88610.0
66-67	98496.0
68-69	95601.0
70-71	115392.0
72-73	76066.0
74-75	76865.0
76-77	81798.0
78-79	85182.0
80-81	86396.0
82-83	82355.0
84-85	82388.0
86-87	85305.0
88-89	89932.0
90-91	92771.0
92-93	90520.0
94-95	98010.0
96-97	164212.0
98-99	961010.0
100-101	7803971.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.91264744709903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07366985936815	72.14347468562616
2	7.924229437339584	12.981892243814425
3	2.157392401422867	5.301531695484051
4	0.7951662353129302	2.6053668598010016
5	0.39955950634015974	1.6364488488489217
6	0.20706004200863998	1.0176501732861145
7	0.12181221904748558	0.6984572947509842
8	0.0831404798312787	0.5448205450401744
9	0.059596377626484565	0.4393527362678169
>10	0.17314457023253285	2.1688851370066824
>50	0.004129432864457385	0.22771785313561646
>100	0.001040601150267463	0.15584356228563465
>500	1.961248800392164E-5	0.01503694123238448
>1k	3.9224967225679346E-5	0.06352142341996402
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7458324578710986E-5	0.0	0.0	0.0
3	0.0	1.7458324578710986E-5	0.0	8.729162289355493E-6	0.0
4	0.0	6.110413602548846E-5	0.0	8.729162289355493E-6	0.0
5	0.0	6.110413602548846E-5	0.0	8.729162289355493E-6	0.0
6	0.0	6.983329831484394E-5	0.0	8.729162289355493E-6	8.729162289355493E-6
7	0.0	6.983329831484394E-5	0.0	1.7458324578710986E-5	8.729162289355493E-6
8	0.0	6.983329831484394E-5	0.0	1.7458324578710986E-5	8.729162289355493E-6
9	0.0	1.396665966296879E-4	0.0	3.491664915742197E-5	8.729162289355493E-6
10-11	0.0	1.483957589190434E-4	0.0	3.9281230302099716E-5	8.729162289355493E-6
12-13	0.0	1.483957589190434E-4	0.0	4.364581144677747E-5	8.729162289355493E-6
14-15	4.3645811446777465E-6	2.5751028753598706E-4	0.0	5.237497373613296E-5	3.491664915742197E-5
16-17	8.729162289355493E-6	2.6187486868066483E-4	0.0	5.237497373613296E-5	4.364581144677747E-5
18-19	8.729162289355493E-6	3.0988526127212E-4	0.0	6.54687171701662E-5	4.364581144677747E-5
20-21	8.729162289355493E-6	3.5789565386357526E-4	0.0	6.983329831484394E-5	4.364581144677747E-5
22-23	8.729162289355493E-6	4.2772895217841917E-4	0.0	6.983329831484394E-5	6.983329831484394E-5
24-25	8.729162289355493E-6	4.888330882039077E-4	0.0	7.41978794595217E-5	1.0038536632758817E-4
26-27	8.729162289355493E-6	5.324788996506851E-4	0.0	1.396665966296879E-4	1.0474994747226592E-4
28-29	8.729162289355493E-6	5.412080619400406E-4	0.0	4.015414653103527E-4	1.0911452861694367E-4
30-31	8.729162289355493E-6	6.066767791102068E-4	0.0	0.0010387703124333037	1.1347910976162141E-4
32-33	8.729162289355493E-6	6.110413602548845E-4	0.0	0.002239030127219684	1.396665966296879E-4
34-35	8.729162289355493E-6	7.026975642931172E-4	0.0	0.003928123030209972	1.571249212083989E-4
36-37	8.729162289355493E-6	7.9435376833135E-4	0.0	0.006533777973582587	1.6585408349775437E-4
38-39	1.3093743434033239E-5	8.161766740547387E-4	0.0	0.011400285949898274	1.8331240807646537E-4
40-41	1.7458324578710986E-5	8.729162289355494E-4	0.0	0.01626679392621396	2.0949989494453185E-4
42-43	1.7458324578710986E-5	9.165620403823268E-4	0.0	0.02091943742644044	2.2259363837856509E-4
44-45	1.7458324578710986E-5	0.001051864055867337	0.0	0.025947434905109205	2.4005196295727606E-4
46-47	1.7458324578710986E-5	0.0011609785844842806	0.0	0.031734869502951894	2.4878112524663154E-4
48-49	1.7458324578710986E-5	0.0011653431656289584	0.0	0.037661970697424274	2.793331932593758E-4
50-51	1.7458324578710986E-5	0.0013093743434033239	0.0	0.043720009326236986	2.793331932593758E-4
52-53	1.7458324578710986E-5	0.001326832667982035	0.0	0.04911463162105868	3.011560989827645E-4
54-55	1.7458324578710986E-5	0.0013355618302713906	0.0	0.055006816166373644	3.317081669955088E-4
56-57	1.7458324578710986E-5	0.001361749317139457	0.0	0.06090772987397795	3.4916649157421974E-4
58-59	1.7458324578710986E-5	0.001361749317139457	0.0	0.06612340434186786	3.5789565386357526E-4
60-61	1.7458324578710986E-5	0.0013704784794288127	0.0	0.07129106841716631	3.5789565386357526E-4
62-63	1.7458324578710986E-5	0.0014097597097309123	0.0	0.07592625359281409	3.6662481615293074E-4
64-65	1.7458324578710986E-5	0.0015799783743733443	0.0	0.08050906379472572	3.6662481615293074E-4
66-67	1.7458324578710986E-5	0.001601801280096733	0.0	0.08536247802760738	3.6662481615293074E-4
68-69	1.7458324578710986E-5	0.0016367179292541552	0.0	0.09005876733928063	3.6662481615293074E-4
70-71	1.7458324578710986E-5	0.0016629054161222214	0.0	0.09489035866643888	3.6662481615293074E-4
72-73	1.7458324578710986E-5	0.0016803637407009325	0.0	0.09868317968116386	4.364581144677747E-4
74-75	1.7458324578710986E-5	0.001706551227568999	0.0	0.10169037608984682	4.364581144677747E-4
76-77	1.7458324578710986E-5	0.001758926201305132	0.0	0.10425674980291733	4.364581144677747E-4
78-79	1.7458324578710986E-5	0.001793842850462554	0.0	0.10653506116043912	4.364581144677747E-4
80-81	1.7458324578710986E-5	0.0018113011750412649	0.0	0.10747344610654484	4.626456013358412E-4
82-83	1.7458324578710986E-5	0.0018331240807646536	0.0	0.1078749875718552	4.888330882039077E-4
84-85	1.7458324578710986E-5	0.0018505824053433647	0.0	0.10823288322571877	4.888330882039077E-4
86-87	1.7458324578710986E-5	0.001911686541368853	0.0	0.1084205602149399	4.975622504932632E-4
88-89	1.7458324578710986E-5	0.0019727906773943414	0.0	0.10846857060753136	5.193851562166518E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16475	0.0	51.42661	1
CTTATAC	11545	0.0	42.312565	1
GTATCAA	31555	0.0	40.606216	1
TCAACGC	38020	0.0	33.539814	4
ATCAACG	38060	0.0	33.460125	3
TATCAAC	39060	0.0	32.681515	2
CAACGCA	39145	0.0	32.564777	5
TATACAC	16295	0.0	32.489994	3
AACGCAG	40270	0.0	31.680159	6
TTATACA	17410	0.0	29.43392	2
ACGCAGA	45660	0.0	27.902311	7
CGCAGAG	46270	0.0	27.515774	8
GTGGTAT	7855	0.0	26.993126	1
TGGTATC	7455	0.0	25.754992	2
ACACATC	20340	0.0	24.10893	6
GCAGAGT	53330	0.0	23.889463	9
TACACAT	22135	0.0	23.720352	5
ACATCTC	21095	0.0	23.63807	8
CACATCT	22965	0.0	21.296333	7
GAGTACT	32095	0.0	21.162766	12-13
>>END_MODULE