FastQCFastQC Report
Fri 27 May 2016
SRR2139322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11089420
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148350.049.8381
GTATCAA282800.039.5326461
TCAACGC333000.033.401784
ATCAACG338650.032.819283
CAACGCA343700.032.336485
TATCAAC349650.032.0493772
AACGCAG354950.031.3046976
CTTATAC86050.028.5047051
ACGCAGA402800.027.5642097
CGCAGAG408900.027.1423178
GTGGTAT72850.024.8470781
TGGTATC71050.024.3073462
GCAGAGT476550.023.3534369
TATACAC131000.022.2283633
GAGTACT286300.021.15301112-13
CAGAGTA465650.019.88859710-11
AGAGTAC432450.019.44519610-11
TATGCCG73100.019.222942-43
GTACTTT313950.019.1377214-15
TACATGG322400.019.1082322
Produced by FastQC (version 0.11.5)