##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139322_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11089420
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.151503775670864	31.0	25.0	33.0	18.0	33.0
2	30.577648154727658	33.0	29.0	33.0	25.0	33.0
3	30.376648192601596	33.0	29.0	33.0	25.0	33.0
4	31.753701095278203	33.0	32.0	33.0	29.0	34.0
5	31.85647130327826	33.0	32.0	33.0	30.0	34.0
6	34.990518530274805	37.0	34.0	38.0	29.0	38.0
7	36.11654450818889	38.0	37.0	38.0	33.0	38.0
8	36.195438174404075	38.0	37.0	38.0	33.0	38.0
9	36.619329234531655	38.0	38.0	38.0	34.0	38.0
10-11	36.76623863105554	38.0	38.0	38.0	34.5	38.0
12-13	36.841888890492015	38.0	38.0	38.0	35.0	38.0
14-15	36.88523236562417	38.0	38.0	38.0	35.0	38.0
16-17	36.89141699926597	38.0	38.0	38.0	35.0	38.0
18-19	36.909588238158534	38.0	38.0	38.0	35.0	38.0
20-21	36.924155189829705	38.0	38.0	38.0	35.0	38.0
22-23	36.93884784203907	38.0	38.0	38.0	35.0	38.0
24-25	36.932416326981084	38.0	38.0	38.0	35.0	38.0
26-27	36.912436100607465	38.0	38.0	38.0	35.0	38.0
28-29	36.892890573837235	38.0	38.0	38.0	35.0	38.0
30-31	36.88386885411522	38.0	38.0	38.0	35.0	38.0
32-33	36.855082182438366	38.0	38.0	38.0	35.0	38.0
34-35	36.83176719364584	38.0	38.0	38.0	35.0	38.0
36-37	36.802531625282896	38.0	38.0	38.0	35.0	38.0
38-39	36.7754546116829	38.0	38.0	38.0	35.0	38.0
40-41	36.78008782479763	38.0	38.0	38.0	35.0	38.0
42-43	36.73172857043695	38.0	38.0	38.0	35.0	38.0
44-45	36.69547946342835	38.0	38.0	38.0	34.5	38.0
46-47	36.65291892323845	38.0	38.0	38.0	34.0	38.0
48-49	36.618388673316524	38.0	38.0	38.0	34.0	38.0
50-51	36.59020864053144	38.0	38.0	38.0	34.0	38.0
52-53	36.564495270048965	38.0	38.0	38.0	34.0	38.0
54-55	36.55009871917756	38.0	38.0	38.0	34.0	38.0
56-57	36.5330787478467	38.0	38.0	38.0	34.0	38.0
58-59	36.519069976777395	38.0	38.0	38.0	34.0	38.0
60-61	36.5184892720928	38.0	38.0	38.0	34.0	38.0
62-63	36.50349439001094	38.0	38.0	38.0	34.0	38.0
64-65	36.49201918156763	38.0	38.0	38.0	34.0	38.0
66-67	36.46370926821331	38.0	38.0	38.0	34.0	38.0
68-69	36.44984027771821	38.0	38.0	38.0	34.0	38.0
70-71	36.450676526239164	38.0	38.0	38.0	34.0	38.0
72-73	36.43077545448586	38.0	38.0	38.0	34.0	38.0
74-75	36.41931005173994	38.0	38.0	38.0	34.0	38.0
76-77	36.40815489820777	38.0	38.0	38.0	34.0	38.0
78-79	36.395025212999556	38.0	38.0	38.0	34.0	38.0
80-81	36.38834269185299	38.0	38.0	38.0	34.0	38.0
82-83	36.365292759023006	38.0	38.0	38.0	34.0	38.0
84-85	36.35421348264915	38.0	38.0	38.0	34.0	38.0
86-87	36.33756208478371	38.0	38.0	38.0	33.5	38.0
88-89	36.33817386058436	38.0	38.0	38.0	33.5	38.0
90-91	36.340892465243485	38.0	38.0	38.0	34.0	38.0
92-93	36.310268768425985	38.0	37.0	38.0	33.0	38.0
94-95	36.29957732180668	38.0	37.0	38.0	33.0	38.0
96-97	36.29498759329084	38.0	37.0	38.0	33.0	38.0
98-99	36.32131057010368	38.0	37.0	38.0	33.0	38.0
100-101	35.77745827297881	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	35.0
19	81.0
20	156.0
21	554.0
22	2286.0
23	6469.0
24	14658.0
25	27996.0
26	46188.0
27	70124.0
28	99839.0
29	134673.0
30	173894.0
31	220676.0
32	283860.0
33	371583.0
34	520226.0
35	824329.0
36	1820276.0
37	6471513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.392294637591505	20.241780002921704	14.27972788477666	26.086197474710126
2	13.882222875497547	20.34044161011126	43.313581774339866	22.463753740051327
3	17.878017405215253	25.69215737286848	29.75532511122303	26.674500110693238
4	12.363072189528397	18.401963312779205	38.90898712466477	30.325977373027623
5	13.82238966261695	36.64149827230844	35.3640063025957	14.172105762478909
6	29.660947100930436	37.94776462610308	18.69773171184787	13.693556561118617
7	26.355968120965752	32.809497701412695	22.677020078597437	18.157514099024112
8	23.99565531831241	36.964331768478424	20.952556580957346	18.087456332251822
9	25.124875782502603	17.517814277031622	21.243284139296737	36.11402580116904
10-11	24.114985274252394	26.967348157072234	28.521176039865026	20.396490528810343
12-13	25.068312860366003	24.221861918837956	28.788015964766416	21.92180925602962
14-15	22.14941706578597	25.63970456153868	26.557597803166548	25.6532805695088
16-17	21.67900575503498	28.968237292843092	27.14858847441976	22.204168477702172
18-19	21.661673919826285	27.831865868548583	29.165344084722193	21.34111612690294
20-21	22.445646143275237	26.959372258173342	29.115432359306798	21.479549239244623
22-23	22.089669933739287	26.965298303071382	29.03588020224695	21.909151560942377
24-25	21.938099195531162	27.11176282859095	29.12385712126937	21.826280854608513
26-27	21.882272438071116	27.30509071059614	29.07186061255546	21.740776238777283
28-29	21.74975632852216	27.232873239176104	29.22160694375176	21.79576348854998
30-31	22.094680795638183	27.251074576022955	28.946689308994543	21.70755531934432
32-33	22.058078572902982	27.195443353122723	28.97009932225914	21.776378751715153
34-35	22.020182769823325	27.19435049552514	28.99206633264184	21.793400402009695
36-37	21.903354509048288	27.259762431231273	28.985479646101687	21.85140341361876
38-39	21.76007697807009	27.23638517967577	29.13455746194477	21.868980380309367
40-41	22.07259235579731	27.106221893714665	29.013031061499166	21.80815468898886
42-43	22.060069004548538	27.135340211697084	28.845883949614375	21.958706834140003
44-45	22.003983169538692	27.336944222620495	28.716184956706858	21.94288765113395
46-47	22.19050175256895	27.205041557845306	28.357630303074266	22.24682638651148
48-49	22.04399207063466	27.305953949730366	28.434592471553493	22.215461508081482
50-51	21.958148807404772	27.485790910169694	28.391323128040817	22.164737154384717
52-53	22.184320716847576	27.429613749156072	28.338434769185977	22.04763076481037
54-55	22.127734399197113	27.301786498891122	28.268502059670297	22.301977042241468
56-57	22.238042078053734	27.53339588927453	28.093230766358012	22.135331266313724
58-59	22.03241673400243	27.68523578363756	28.33881931685124	21.94352816550877
60-61	22.19942309985409	27.767028950514483	28.125527750523528	21.908020199107902
62-63	22.036820303728536	27.968079244663492	28.035324558531922	21.95977589307605
64-65	22.014090776833033	27.92530843780387	27.998224353219413	22.062376432143683
66-67	22.103961676744696	27.93903005440372	27.86633266645596	22.090675602395624
68-69	22.083059351796642	27.78245349334614	28.031523633344356	22.102963521512862
70-71	22.086597949930166	27.4755464442248	28.120764895853632	22.3170907099914
72-73	22.17491698572001	27.428714090345036	28.08943536903113	22.306933554903825
74-75	22.030043337902423	27.555299088796016	28.089397340464608	22.325260232836957
76-77	22.192329102975695	27.523748709729766	28.00841561507203	22.27550657222251
78-79	22.151688679289045	27.580255860626128	27.931066874542182	22.336988585542645
80-81	22.199139974926968	27.598359964057416	27.890841441763637	22.311658619251975
82-83	22.23488801890426	27.572556769876073	27.95911535897128	22.23343985224838
84-85	22.34448433385942	27.515849826728683	27.943109435458798	22.196556403953103
86-87	22.153591529560305	27.59041131335535	27.95167850787516	22.304318649209183
88-89	22.213627165024143	27.603016099943346	27.9282032052805	22.25515352975201
90-91	22.289389228345406	27.591162830761707	27.935057753918567	22.18439018697432
92-93	22.318435514371007	27.5676941436723	27.888915967250906	22.224954374705785
94-95	22.158413546747152	27.69914465785889	27.896830782683253	22.2456110127107
96-97	22.205173615750894	27.723229689385743	27.90455027536022	22.167046419503148
98-99	22.48878663155751	28.26198813388665	27.942384099389212	21.306841135166625
100-101	23.054448191377798	30.809830415535007	25.286135088436012	20.849586304651186
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2184.0
1	2128.0
2	2810.0
3	3771.0
4	4825.0
5	6927.5
6	8903.0
7	11253.0
8	14228.0
9	15914.0
10	15605.0
11	15028.5
12	16298.5
13	20029.0
14	27730.0
15	37301.0
16	44805.5
17	49327.5
18	49344.5
19	45252.5
20	40283.5
21	36213.5
22	35093.0
23	38855.0
24	47290.5
25	61112.5
26	80467.0
27	102222.5
28	125543.0
29	149726.5
30	172519.5
31	196070.0
32	221782.0
33	249803.5
34	276730.0
35	300096.5
36	328487.5
37	353988.5
38	370444.0
39	384363.0
40	403248.5
41	422438.5
42	432063.5
43	443803.5
44	466379.5
45	477958.0
46	471661.5
47	466631.5
48	459738.5
49	449844.5
50	439081.5
51	424318.5
52	403894.0
53	381222.5
54	359435.0
55	334240.0
56	306783.5
57	277418.5
58	247145.5
59	215665.0
60	180789.0
61	145963.0
62	113944.5
63	87001.0
64	65120.0
65	48063.0
66	35561.5
67	27114.5
68	20621.0
69	14873.0
70	10451.0
71	7269.5
72	4982.0
73	3323.5
74	2128.5
75	1279.5
76	786.0
77	466.5
78	293.0
79	196.5
80	144.5
81	95.5
82	59.0
83	37.5
84	28.5
85	22.5
86	16.0
87	14.0
88	11.0
89	7.0
90	5.0
91	4.0
92	2.5
93	2.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0019387848958737246
4	0.0
5	0.06314126437631545
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.607041666741814E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.510126316009758E-6
28-29	4.511390267920641E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.3699113671911797E-5
40-41	0.0
42-43	0.0
44-45	9.289973805060862E-6
46-47	2.8080336907882223E-4
48-49	0.0
50-51	8.54395852420774E-5
52-53	3.343219232604144E-5
54-55	0.001989492500165911
56-57	0.0025299734125432695
58-59	8.231708267479667E-4
60-61	0.0028938836885363016
62-63	0.004770905182951373
64-65	0.0023028166033375523
66-67	0.00755494391870379
68-69	0.0044195821876678995
70-71	0.0017964659228277028
72-73	7.980345901142461E-4
74-75	7.1067346997319E-4
76-77	0.0
78-79	5.271389863612803E-6
80-81	2.445935909992111E-4
82-83	0.0
84-85	0.0011250379632702149
86-87	0.0010965224526883295
88-89	1.4862277058275372E-4
90-91	4.779228539152107E-4
92-93	2.3568032971004756E-4
94-95	5.6669565881657175E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	135.0
20-21	448.0
22-23	975.0
24-25	1254.0
26-27	2447.0
28-29	5581.0
30-31	16162.0
32-33	21160.0
34-35	29532.0
36-37	47641.0
38-39	57699.0
40-41	57050.0
42-43	65878.0
44-45	79361.0
46-47	79934.0
48-49	73607.0
50-51	65914.0
52-53	63475.0
54-55	67433.0
56-57	70975.0
58-59	70784.0
60-61	70749.0
62-63	69942.0
64-65	78023.0
66-67	84872.0
68-69	84169.0
70-71	94526.0
72-73	72981.0
74-75	73756.0
76-77	77660.0
78-79	81253.0
80-81	82075.0
82-83	78738.0
84-85	78004.0
86-87	80678.0
88-89	85483.0
90-91	87510.0
92-93	86158.0
94-95	94114.0
96-97	158697.0
98-99	945297.0
100-101	7647290.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.03660240192421
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.07833505677175	72.25647353275848
2	8.033882584259214	13.181448626172331
3	2.06448459777903	5.080899063386842
4	0.8021751113099619	2.6323088265301857
5	0.3964007289723892	1.6259684497270406
6	0.19956173768543375	0.9822820157482216
7	0.11794383226123492	0.6772997891081921
8	0.07336777934898274	0.4815074674851683
9	0.056014052924619404	0.41356823298276085
>10	0.17278234602877918	2.1671747711516454
>50	0.003971402264411334	0.22287321393835752
>100	9.191413561551613E-4	0.12302746710785985
>500	1.2122217419432944E-4	0.07634160540570673
>1k	4.040686381058491E-5	0.07882693849714142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.017604166854534E-6
2	0.0	9.017604166854534E-6	0.0	0.0	9.017604166854534E-6
3	0.0	9.017604166854534E-6	0.0	1.803520833370907E-5	9.017604166854534E-6
4	0.0	6.312322916798174E-5	0.0	1.803520833370907E-5	9.017604166854534E-6
5	9.017604166854534E-6	7.214083333483627E-5	0.0	2.70528125005636E-5	9.017604166854534E-6
6	1.803520833370907E-5	7.214083333483627E-5	0.0	2.70528125005636E-5	9.017604166854534E-6
7	1.803520833370907E-5	7.214083333483627E-5	0.0	2.70528125005636E-5	9.017604166854534E-6
8	1.803520833370907E-5	8.11584375016908E-5	0.0	2.70528125005636E-5	2.70528125005636E-5
9	1.803520833370907E-5	1.5329927083652706E-4	0.0	4.508802083427267E-5	2.70528125005636E-5
10-11	4.508802083427267E-5	1.5329927083652706E-4	0.0	7.664963541826353E-5	2.70528125005636E-5
12-13	5.41056250011272E-5	1.5329927083652706E-4	0.0	8.11584375016908E-5	3.156161458399087E-5
14-15	5.41056250011272E-5	2.2544010417136333E-4	0.0	9.017604166854534E-5	4.959682291769993E-5
16-17	6.312322916798174E-5	2.3445770833821787E-4	0.0	9.919364583539987E-5	6.312322916798174E-5
18-19	7.664963541826353E-5	2.5249291667192694E-4	0.0	1.1722885416910893E-4	7.214083333483627E-5
20-21	1.082112500022544E-4	2.70528125005636E-4	0.0	1.1722885416910893E-4	8.566723958511807E-5
22-23	1.35264062502818E-4	3.2914255209019045E-4	0.0	1.487904687530998E-4	9.46848437519726E-5
24-25	1.35264062502818E-4	3.787393750078904E-4	0.0	1.5329927083652706E-4	1.1722885416910893E-4
26-27	1.623168750033816E-4	3.877569791747449E-4	0.0	1.9838729167079972E-4	1.1722885416910893E-4
28-29	1.6682567708680888E-4	3.877569791747449E-4	0.0	3.652129687576086E-4	1.1722885416910893E-4
30-31	1.7133447917023613E-4	4.463714062592994E-4	0.0	9.017604166854533E-4	1.217376562525362E-4
32-33	1.8035208333709067E-4	4.73424218759863E-4	0.0	0.002209313020879361	1.35264062502818E-4
34-35	1.8486088542051795E-4	5.275298437609902E-4	0.0	0.004071448281334822	1.487904687530998E-4
36-37	1.9838729167079975E-4	5.771266666786902E-4	0.0	0.006839852760559163	1.5329927083652706E-4
38-39	2.0740489583765426E-4	5.861442708455447E-4	0.0	0.011276514010651596	1.623168750033816E-4
40-41	2.1191369792108154E-4	6.492675000135264E-4	0.0	0.016416548385758678	1.7133447917023613E-4
42-43	2.2093130208793605E-4	6.582851041803809E-4	0.0	0.02111472015668989	1.893696875039452E-4
44-45	2.2544010417136333E-4	7.259171354317899E-4	0.0	0.0262592633338804	2.0740489583765426E-4
46-47	2.2544010417136333E-4	7.93549166683199E-4	0.0	0.03192682755274848	2.0740489583765426E-4
48-49	2.3445770833821787E-4	7.93549166683199E-4	0.0	0.037747691042453076	2.2544010417136333E-4
50-51	2.434753125050724E-4	8.747076041848898E-4	0.0	0.04422233083425463	2.2544010417136333E-4
52-53	2.5249291667192694E-4	8.837252083517443E-4	0.0	0.050061229532292945	2.3896651042164512E-4
54-55	2.570017187553542E-4	8.837252083517443E-4	0.0	0.05587307541783069	2.5249291667192694E-4
56-57	2.70528125005636E-4	8.927428125185988E-4	0.0	0.06100409218877092	2.5249291667192694E-4
58-59	2.70528125005636E-4	9.017604166854533E-4	0.0	0.06564364953261757	2.570017187553542E-4
60-61	2.70528125005636E-4	9.378308333528715E-4	0.0	0.07006678437645973	2.6151052083878145E-4
62-63	2.750369270890633E-4	9.693924479368623E-4	0.0	0.07461165687655441	2.7954572917249053E-4
64-65	2.7954572917249053E-4	0.0011091653125231077	0.0	0.079007738907896	2.7954572917249053E-4
66-67	2.7954572917249053E-4	0.0011226917187733894	0.0	0.08370591067882721	2.7954572917249053E-4
68-69	2.840545312559178E-4	0.0011587621354408077	0.0	0.08825980078308875	2.840545312559178E-4
70-71	2.885633333393451E-4	0.0011813061458579438	0.0	0.09255668916859494	3.2012494792333594E-4
72-73	2.885633333393451E-4	0.0011948325521082257	0.0	0.09641622375200867	3.6972177084103585E-4
74-75	2.885633333393451E-4	0.001199341354191653	0.0	0.09932440109581925	3.6972177084103585E-4
76-77	2.885633333393451E-4	0.001235411770859071	0.0	0.1020747703667099	3.6972177084103585E-4
78-79	2.885633333393451E-4	0.0012624645833596347	0.0	0.10413078411675272	3.6972177084103585E-4
80-81	2.885633333393451E-4	0.0012624645833596347	0.0	0.10522191422094213	3.787393750078904E-4
82-83	2.885633333393451E-4	0.0012804997916933437	0.0	0.10558712718969973	4.1030098959188126E-4
84-85	2.975809375061996E-4	0.0012895173958601984	0.0	0.10590725213762306	4.148097916753085E-4
86-87	2.975809375061996E-4	0.0012985350000270528	0.0	0.10602448099179218	4.148097916753085E-4
88-89	2.975809375061996E-4	0.001370675833361889	0.0	0.10607407781470987	4.2833619792559033E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14835	0.0	49.838	1
GTATCAA	28280	0.0	39.532646	1
TCAACGC	33300	0.0	33.40178	4
ATCAACG	33865	0.0	32.81928	3
CAACGCA	34370	0.0	32.33648	5
TATCAAC	34965	0.0	32.049377	2
AACGCAG	35495	0.0	31.304697	6
CTTATAC	8605	0.0	28.504705	1
ACGCAGA	40280	0.0	27.564209	7
CGCAGAG	40890	0.0	27.142317	8
GTGGTAT	7285	0.0	24.847078	1
TGGTATC	7105	0.0	24.307346	2
GCAGAGT	47655	0.0	23.353436	9
TATACAC	13100	0.0	22.228363	3
GAGTACT	28630	0.0	21.153011	12-13
CAGAGTA	46565	0.0	19.888597	10-11
AGAGTAC	43245	0.0	19.445196	10-11
TATGCCG	7310	0.0	19.2229	42-43
GTACTTT	31395	0.0	19.13772	14-15
TACATGG	32240	0.0	19.108232	2
>>END_MODULE