FastQCFastQC Report
Fri 27 May 2016
SRR2139321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9044260
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142650.050.170971
GTATCAA261350.039.2020871
TCAACGC294350.034.3141564
ATCAACG296400.034.0475583
CAACGCA303900.033.2069245
TATCAAC308750.032.6856582
AACGCAG314250.032.1529776
ACGCAGA366100.027.563237
CGCAGAG369400.027.3178
TGGTATC67900.024.9111732
GTGGTAT72900.024.829781
GCAGAGT428650.023.500149
GAGTACT264200.021.75638212-13
CAGAGTA419550.020.2890610-11
GTACTTT288100.019.76901214-15
GTACATG292750.018.9543821
TACATGG282400.018.7934022
AGAGTAC387250.018.60788510-11
ACATGGG285750.018.1118263
GTGTAGC58850.017.917111
Produced by FastQC (version 0.11.5)