##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9044260
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.343108778385407	32.0	18.0	33.0	18.0	33.0
2	28.35444436581876	30.0	27.0	33.0	18.0	33.0
3	28.58099756088392	31.0	27.0	33.0	18.0	33.0
4	31.345521690000066	33.0	31.0	33.0	28.0	33.0
5	31.92758313007366	33.0	32.0	33.0	30.0	34.0
6	35.95521269844078	38.0	36.0	38.0	31.0	38.0
7	36.26218231231743	38.0	37.0	38.0	33.0	38.0
8	36.60742990581872	38.0	37.0	38.0	34.0	38.0
9	36.84672897506264	38.0	38.0	38.0	35.0	38.0
10-11	36.89570633750024	38.0	38.0	38.0	35.0	38.0
12-13	36.96000900018355	38.0	38.0	38.0	35.0	38.0
14-15	36.95674051829558	38.0	38.0	38.0	35.0	38.0
16-17	36.97256082863606	38.0	38.0	38.0	35.0	38.0
18-19	36.98413015547983	38.0	38.0	38.0	35.5	38.0
20-21	36.99654081163122	38.0	38.0	38.0	36.0	38.0
22-23	37.010888598262454	38.0	38.0	38.0	36.0	38.0
24-25	37.000348541104444	38.0	38.0	38.0	36.0	38.0
26-27	36.971931471274274	38.0	38.0	38.0	36.0	38.0
28-29	36.94809002708234	38.0	38.0	38.0	35.0	38.0
30-31	36.94632748825295	38.0	38.0	38.0	35.0	38.0
32-33	36.919575537434156	38.0	38.0	38.0	35.0	38.0
34-35	36.895512966680414	38.0	38.0	38.0	35.0	38.0
36-37	36.86454511954722	38.0	38.0	38.0	35.0	38.0
38-39	36.839891230636155	38.0	38.0	38.0	35.0	38.0
40-41	36.83737568647213	38.0	38.0	38.0	35.0	38.0
42-43	36.78291058916474	38.0	38.0	38.0	35.0	38.0
44-45	36.742052765527205	38.0	38.0	38.0	35.0	38.0
46-47	36.69679378719883	38.0	38.0	38.0	35.0	38.0
48-49	36.65775132075926	38.0	38.0	38.0	34.0	38.0
50-51	36.62644236740188	38.0	38.0	38.0	34.0	38.0
52-53	36.60388936848413	38.0	38.0	38.0	34.0	38.0
54-55	36.58804223943302	38.0	38.0	38.0	34.0	38.0
56-57	36.56895043565778	38.0	38.0	38.0	34.0	38.0
58-59	36.5560437206264	38.0	38.0	38.0	34.0	38.0
60-61	36.5570166622125	38.0	38.0	38.0	34.0	38.0
62-63	36.545126973396215	38.0	38.0	38.0	34.0	38.0
64-65	36.53912039534687	38.0	38.0	38.0	34.0	38.0
66-67	36.511914603017	38.0	38.0	38.0	34.0	38.0
68-69	36.49888641845368	38.0	38.0	38.0	34.0	38.0
70-71	36.499932075123915	38.0	38.0	38.0	34.0	38.0
72-73	36.48499148714255	38.0	38.0	38.0	34.0	38.0
74-75	36.476204388924515	38.0	38.0	38.0	34.0	38.0
76-77	36.463700388975305	38.0	38.0	38.0	34.0	38.0
78-79	36.447661288936544	38.0	38.0	38.0	34.0	38.0
80-81	36.44643243825925	38.0	38.0	38.0	34.0	38.0
82-83	36.42487173623347	38.0	38.0	38.0	34.0	38.0
84-85	36.4161955268231	38.0	38.0	38.0	34.0	38.0
86-87	36.40154520647148	38.0	38.0	38.0	34.0	38.0
88-89	36.40100374933263	38.0	38.0	38.0	34.0	38.0
90-91	36.40750662645988	38.0	38.0	38.0	34.0	38.0
92-93	36.37811672446466	38.0	38.0	38.0	34.0	38.0
94-95	36.36755819722319	38.0	38.0	38.0	34.0	38.0
96-97	36.359464648415205	38.0	38.0	38.0	34.0	38.0
98-99	36.38942234434559	38.0	38.0	38.0	34.0	38.0
100-101	35.84865118937999	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	44.0
20	94.0
21	391.0
22	1504.0
23	4692.0
24	10856.0
25	20997.0
26	35800.0
27	54773.0
28	78616.0
29	106479.0
30	137276.0
31	173272.0
32	222372.0
33	292622.0
34	411046.0
35	664007.0
36	1580160.0
37	5249250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91987183031006	19.071632173334248	13.732289872250467	22.276206124105233
2	14.592017478489119	20.276153051769853	41.180372965836895	23.951456503904133
3	19.71271149999851	25.75040504087228	29.486776478361865	25.050106980767346
4	12.855214246383895	18.1869716262027	39.2449575753019	29.712856552111504
5	13.698549669705681	38.06134408741917	34.574234775293114	13.665871467582038
6	29.766570178212483	37.79945512402341	18.69467485454863	13.739299843215477
7	26.40107648386933	33.22351414046036	22.599416646580263	17.775992729090053
8	24.095470497309897	36.996503859906724	20.878203412993436	18.029822229789943
9	25.322027451665473	17.708436068843664	21.40081112219242	35.56872535729844
10-11	24.17536647553255	27.078124689029288	28.405049169307382	20.341459666130785
12-13	25.05160178942224	24.485983375090942	28.802959003832264	21.659455831654554
14-15	22.2676820070636	25.81653054438134	26.687005539150118	25.228781909404947
16-17	21.593568738625383	29.102596564008554	27.325170881863194	21.978663815502873
18-19	21.503251786215788	27.960302998808086	29.449164442419836	21.087280772556298
20-21	22.357810459648235	27.08416767192719	29.429533540687896	21.128488327736676
22-23	22.0163312293946	27.05040313897346	29.335147582650634	21.598118048981306
24-25	21.81254030527254	27.18443311192285	29.423701852979526	21.579324729825082
26-27	21.7217071961659	27.333726487872784	29.349592839514422	21.594973476446892
28-29	21.607875662504135	27.360133897901633	29.411385935221645	21.620604504372587
30-31	22.090403972041585	27.18623526047769	29.29560037767279	21.42776038980793
32-33	21.800780248547596	27.290768663025418	29.30928642586641	21.599164662560575
34-35	21.89586401870945	27.410898334478528	29.287471122042536	21.405766524769486
36-37	21.887390720952258	27.277312894646254	29.241352035241647	21.593944349159838
38-39	21.660557272547994	27.412624956994357	29.299053349828995	21.62776442062865
40-41	21.99003102086145	27.21277281566564	29.184129360022755	21.613066803450153
42-43	21.879050649985754	27.26232598416138	29.076254303599285	21.782369062253583
44-45	21.889800236584904	27.45881808631159	28.897401437690775	21.753980239412734
46-47	22.026122215736027	27.380452112849852	28.581177357768883	22.012248313645237
48-49	21.868663714439897	27.527247631575197	28.627854577278633	21.976234076706273
50-51	21.83830703807969	27.67096460288166	28.539135919561154	21.951592439477494
52-53	22.041038449380817	27.634597107752086	28.415933406512007	21.90843103635509
54-55	21.995867445716815	27.514195389663126	28.419417655242384	22.07051950937767
56-57	22.021885775131032	27.71440538236691	28.318676942952454	21.9450318995496
58-59	21.917386305422706	27.799779732572418	28.4538714652578	21.828962496747074
60-61	22.024402686369033	27.806466000007408	28.408016872042964	21.7611144415806
62-63	21.89327255348518	27.933333080865413	28.343383059294435	21.830011306354972
64-65	21.872220167064366	27.884857754235632	28.27784467629207	21.965077402407935
66-67	21.964709009670226	27.984531246028443	28.084971842437607	21.965787901863724
68-69	21.892530493143337	27.87086756053993	28.21734718039415	22.019254765922586
70-71	21.905170616094367	27.556930576831174	28.309000140385	22.228898666689464
72-73	22.004308712230618	27.53455965784101	28.285664735675297	22.17546689425307
74-75	21.84698759705536	27.650756015455514	28.258388903541547	22.243867483947575
76-77	22.03981848302585	27.615257941938044	28.151121919053086	22.19380165598302
78-79	22.063028516667025	27.70767644951679	28.015792749363584	22.213502284452606
80-81	22.05881746492406	27.70190248740421	28.023807768717905	22.215472278953822
82-83	22.069234252064835	27.68340679420391	28.123964057452394	22.123394896278857
84-85	22.22307551729066	27.68065685431215	28.023653077192794	22.0726145512044
86-87	22.044135357910918	27.7095588327609	28.094674732942387	22.15163107638579
88-89	22.114820105398923	27.785482533494353	27.979343698818766	22.120353662287958
90-91	22.16597612072924	27.767342627096646	28.039034155862186	22.027647096311927
92-93	22.181894257580794	27.7530806082004	28.023658549147246	22.04136658507156
94-95	22.128821288048204	27.88998980296832	27.940710789579338	22.040478119404145
96-97	22.15720528139035	27.86203237978752	28.019077486134584	21.96168485268754
98-99	22.300755755509634	28.417696583918765	28.093618591917185	21.187929068654416
100-101	22.93815499677496	30.958206279054814	25.476895276286943	20.626743447883282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1952.0
1	1802.0
2	2351.0
3	3366.5
4	4208.5
5	6001.5
6	8012.0
7	10332.0
8	13870.0
9	16135.5
10	15184.5
11	13803.0
12	14564.5
13	17536.5
14	23703.5
15	31280.5
16	37765.0
17	42487.5
18	43516.0
19	40718.5
20	36493.0
21	32588.5
22	31228.0
23	34240.5
24	41247.5
25	52813.0
26	68182.5
27	86306.0
28	106712.5
29	127355.0
30	145751.0
31	165172.0
32	187288.0
33	209595.5
34	231228.5
35	250054.5
36	270500.0
37	289988.0
38	303944.5
39	313919.0
40	326065.0
41	338768.0
42	347059.5
43	353359.5
44	359640.0
45	368773.0
46	371812.5
47	368577.5
48	367441.5
49	361541.0
50	350905.0
51	338838.5
52	321488.5
53	302234.5
54	284072.0
55	264662.0
56	244397.5
57	222321.0
58	199580.5
59	174343.5
60	144921.0
61	116689.5
62	90254.5
63	68148.0
64	51024.5
65	37479.0
66	27483.0
67	20649.5
68	15503.0
69	11352.0
70	8142.0
71	5652.0
72	3954.5
73	2576.0
74	1610.5
75	1012.5
76	610.5
77	345.0
78	213.5
79	141.0
80	88.5
81	63.0
82	45.5
83	31.0
84	21.0
85	11.5
86	9.0
87	10.5
88	9.0
89	5.0
90	2.0
91	3.5
92	5.5
93	4.5
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012272977557036175
4	0.0
5	0.05080570439151462
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.764184134467607E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.1060235257840075E-5
28-29	1.1063746597275586E-5
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.1192098378544747E-5
40-41	0.0
42-43	0.0
44-45	1.1370446356536604E-5
46-47	2.5766569765278E-4
48-49	0.0
50-51	4.642128722399716E-5
52-53	1.750592604773259E-5
54-55	0.0018067279734049642
56-57	0.0024067874236862567
58-59	7.357224456221014E-4
60-61	0.0028247475837210696
62-63	0.004676448940300441
64-65	0.0022144390136235193
66-67	0.007350555616065242
68-69	0.004245261081406229
70-71	0.0018585488227765904
72-73	7.74380640683823E-4
74-75	6.793365021026408E-4
76-77	0.0
78-79	0.0
80-81	2.2552262774842009E-4
82-83	0.0
84-85	9.82881416471035E-4
86-87	0.0011167060211929659
88-89	1.2667162374954273E-4
90-91	4.6434728115918306E-4
92-93	2.5819735845077237E-4
94-95	4.116961504008377E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	110.0
20-21	357.0
22-23	885.0
24-25	1100.0
26-27	2252.0
28-29	4889.0
30-31	12721.0
32-33	16872.0
34-35	23054.0
36-37	36134.0
38-39	42367.0
40-41	43628.0
42-43	50382.0
44-45	61144.0
46-47	62167.0
48-49	56612.0
50-51	50190.0
52-53	44180.0
54-55	46864.0
56-57	47927.0
58-59	53924.0
60-61	54909.0
62-63	53480.0
64-65	59613.0
66-67	64119.0
68-69	64579.0
70-71	69169.0
72-73	57319.0
74-75	59169.0
76-77	62252.0
78-79	65400.0
80-81	66396.0
82-83	63939.0
84-85	63045.0
86-87	66222.0
88-89	69384.0
90-91	71263.0
92-93	71106.0
94-95	76731.0
96-97	129585.0
98-99	777575.0
100-101	6321246.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.78168113868354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.50420933505544	73.26527236605858
2	7.78474767118444	12.88868998922199
3	1.9844090426272036	4.928181498464563
4	0.7655033955725058	2.5347863201145087
5	0.3645411867824054	1.508866614306918
6	0.1933483098603434	0.96034188813374
7	0.11971424098925966	0.6937102287732689
8	0.07152673315065397	0.47368825732553144
9	0.04839855835439866	0.36058626227392176
>10	0.159251550131437	1.9923618381466734
>50	0.003434230437045635	0.18991877999660842
>100	7.981352875587372E-4	0.12101526703576669
>500	9.40885169958818E-5	0.05428794877240712
>1k	2.3522050257037626E-5	0.028292741375462765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2113473075740856E-5	0.0	0.0	0.0
3	0.0	2.2113473075740856E-5	0.0	0.0	0.0
4	0.0	4.422694615148171E-5	0.0	0.0	0.0
5	0.0	4.422694615148171E-5	0.0	0.0	0.0
6	0.0	4.422694615148171E-5	0.0	1.1056736537870428E-5	0.0
7	0.0	4.422694615148171E-5	0.0	1.1056736537870428E-5	1.1056736537870428E-5
8	0.0	4.422694615148171E-5	0.0	2.2113473075740856E-5	1.1056736537870428E-5
9	0.0	9.951062884083386E-5	0.0	3.317020961361129E-5	1.1056736537870428E-5
10-11	0.0	9.951062884083386E-5	0.0	4.422694615148171E-5	1.1056736537870428E-5
12-13	0.0	9.951062884083386E-5	0.0	6.081205095828736E-5	1.1056736537870428E-5
14-15	0.0	1.2162410191657471E-4	0.0	7.739715576509301E-5	1.658510480680564E-5
16-17	0.0	1.2162410191657471E-4	0.0	1.0503899710976907E-4	2.2113473075740856E-5
18-19	0.0	1.3268083845444513E-4	0.0	1.7137941633699164E-4	2.7641841344676072E-5
20-21	0.0	1.603226797991212E-4	0.0	1.7690778460592685E-4	3.317020961361129E-5
22-23	0.0	1.8243615287486206E-4	0.0	1.7690778460592685E-4	4.422694615148171E-5
24-25	0.0	1.990212576816677E-4	0.0	1.8796452114379727E-4	4.422694615148171E-5
26-27	0.0	2.1007799421953813E-4	0.0	2.6536167690889025E-4	4.422694615148171E-5
28-29	0.0	2.1007799421953813E-4	0.0	5.196666172799101E-4	4.422694615148171E-5
30-31	0.0	2.32191467295279E-4	0.0	0.001393148803771674	4.422694615148171E-5
32-33	0.0	2.4324820383314941E-4	0.0	0.0033723046440504805	4.422694615148171E-5
34-35	0.0	2.9853188652250156E-4	0.0	0.0059982795717947075	4.422694615148171E-5
36-37	0.0	3.538155692118537E-4	0.0	0.010127970668689312	4.422694615148171E-5
38-39	0.0	3.538155692118537E-4	0.0	0.01784557277212287	4.422694615148171E-5
40-41	0.0	4.367410932458819E-4	0.0	0.04433751351686042	4.422694615148171E-5
42-43	0.0	4.588545663216228E-4	0.0	0.0524365730308505	4.422694615148171E-5
44-45	0.0	4.8649640766629883E-4	0.0	0.06355412161967922	4.975531442041693E-5
46-47	0.0	4.975531442041693E-4	0.0	0.07628042537476809	5.5283682689352145E-5
48-49	0.0	4.975531442041693E-4	0.0	0.0875693533799338	5.5283682689352145E-5
50-51	0.0	6.136488778518088E-4	0.0	0.09968200825717086	6.634041922722258E-5
52-53	0.0	6.19177246120744E-4	0.0	0.12094411261949567	6.634041922722258E-5
54-55	0.0	6.302339826586144E-4	0.0	0.17498944081660633	6.634041922722258E-5
56-57	0.0	6.412907191964848E-4	0.0	0.23374493877884978	7.739715576509301E-5
58-59	0.0	6.4681908746542E-4	0.0	0.3003838898925949	8.845389230296343E-5
60-61	0.0	6.523474557343553E-4	0.0	0.31575275368023475	8.845389230296343E-5
62-63	0.0	6.744609288100961E-4	0.0	0.3256982881960492	8.845389230296343E-5
64-65	0.0	8.181985038024117E-4	0.0	0.3335043441917857	8.845389230296343E-5
66-67	0.0	8.181985038024117E-4	0.0	0.33887791814919077	8.845389230296343E-5
68-69	0.0	8.347836086092174E-4	0.0	0.34383686448642564	9.398226057189863E-5
70-71	0.0	8.458403451470878E-4	0.0	0.34864654488039926	9.951062884083386E-5
72-73	0.0	8.51368713416023E-4	0.0	0.3520962466802149	9.951062884083386E-5
74-75	0.0	8.679538182228287E-4	0.0	0.3548770159194893	9.951062884083386E-5
76-77	0.0	9.121807643743104E-4	0.0	0.3575195759520403	9.951062884083386E-5
78-79	0.0	9.453509739879216E-4	0.0	0.3597309232596144	9.951062884083386E-5
80-81	0.0	9.508793422568568E-4	0.0	0.3607481430210985	9.951062884083386E-5
82-83	0.0	9.508793422568568E-4	0.0	0.3611904124826133	1.2162410191657471E-4
84-85	0.0	9.564077105257921E-4	0.0	0.36148341600086686	1.2162410191657471E-4
86-87	0.0	9.84049551870468E-4	0.0	0.3616160968393213	1.2715247018550994E-4
88-89	0.0	0.00102827649802195	0.0	0.3616658521537417	1.4373757499231557E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14265	0.0	50.17097	1
GTATCAA	26135	0.0	39.202087	1
TCAACGC	29435	0.0	34.314156	4
ATCAACG	29640	0.0	34.047558	3
CAACGCA	30390	0.0	33.206924	5
TATCAAC	30875	0.0	32.685658	2
AACGCAG	31425	0.0	32.152977	6
ACGCAGA	36610	0.0	27.56323	7
CGCAGAG	36940	0.0	27.317	8
TGGTATC	6790	0.0	24.911173	2
GTGGTAT	7290	0.0	24.82978	1
GCAGAGT	42865	0.0	23.50014	9
GAGTACT	26420	0.0	21.756382	12-13
CAGAGTA	41955	0.0	20.28906	10-11
GTACTTT	28810	0.0	19.769012	14-15
GTACATG	29275	0.0	18.954382	1
TACATGG	28240	0.0	18.793402	2
AGAGTAC	38725	0.0	18.607885	10-11
ACATGGG	28575	0.0	18.111826	3
GTGTAGC	5885	0.0	17.91711	1
>>END_MODULE