FastQCFastQC Report
Fri 27 May 2016
SRR2139320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10030080
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150500.052.547871
GTATCAA280600.039.445241
TCAACGC318300.034.732034
ATCAACG320250.034.4248853
CAACGCA329950.033.4659045
TATCAAC329350.033.314262
AACGCAG339300.032.5418246
ACGCAGA392800.028.08737
CGCAGAG399600.027.6202878
GTGGTAT70300.027.2056791
TGGTATC66350.026.7804952
GCAGAGT463900.023.754199
GAGTACT276600.022.0231512-13
CAGAGTA448400.020.98510710-11
GTACATG330250.020.1169621
GTACTTT302150.020.00953514-15
TACATGG322850.019.9680372
ACATGGG327050.019.243243
GTGTAGC64150.018.693361
AGAGTAC420050.018.31377210-11
Produced by FastQC (version 0.11.5)