##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10030080
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.188575764101582	31.0	18.0	33.0	18.0	33.0
2	28.16713705174834	29.0	27.0	33.0	18.0	33.0
3	28.397100421930833	30.0	27.0	31.0	18.0	33.0
4	31.287925121235325	33.0	31.0	33.0	28.0	33.0
5	31.895408511198315	33.0	32.0	33.0	30.0	34.0
6	35.94051114248341	38.0	36.0	38.0	31.0	38.0
7	36.25873342984303	38.0	37.0	38.0	33.0	38.0
8	36.61053132178407	38.0	37.0	38.0	34.0	38.0
9	36.853258598136804	38.0	38.0	38.0	35.0	38.0
10-11	36.90035069510911	38.0	38.0	38.0	35.0	38.0
12-13	36.96281136341884	38.0	38.0	38.0	35.0	38.0
14-15	36.957088079058195	38.0	38.0	38.0	35.0	38.0
16-17	36.97287459322358	38.0	38.0	38.0	35.0	38.0
18-19	36.982314448139995	38.0	38.0	38.0	35.5	38.0
20-21	36.99413343531489	38.0	38.0	38.0	36.0	38.0
22-23	37.008878089852445	38.0	38.0	38.0	36.0	38.0
24-25	36.996010348198865	38.0	38.0	38.0	36.0	38.0
26-27	36.966830502013735	38.0	38.0	38.0	35.5	38.0
28-29	36.94327627677279	38.0	38.0	38.0	35.0	38.0
30-31	36.9445515137042	38.0	38.0	38.0	35.0	38.0
32-33	36.92098670331935	38.0	38.0	38.0	35.0	38.0
34-35	36.89626949166902	38.0	38.0	38.0	35.0	38.0
36-37	36.86593166256736	38.0	38.0	38.0	35.0	38.0
38-39	36.84395099777251	38.0	38.0	38.0	35.0	38.0
40-41	36.837060814676164	38.0	38.0	38.0	35.0	38.0
42-43	36.78010581923003	38.0	38.0	38.0	35.0	38.0
44-45	36.74060107446056	38.0	38.0	38.0	35.0	38.0
46-47	36.69600018879292	38.0	38.0	38.0	35.0	38.0
48-49	36.65846685948616	38.0	38.0	38.0	34.0	38.0
50-51	36.627941979460516	38.0	38.0	38.0	34.0	38.0
52-53	36.60347874517305	38.0	38.0	38.0	34.0	38.0
54-55	36.58760759244788	38.0	38.0	38.0	34.0	38.0
56-57	36.56674574935303	38.0	38.0	38.0	34.0	38.0
58-59	36.55253041639634	38.0	38.0	38.0	34.0	38.0
60-61	36.55080591669925	38.0	38.0	38.0	34.0	38.0
62-63	36.537064163966456	38.0	38.0	38.0	34.0	38.0
64-65	36.52932725077645	38.0	38.0	38.0	34.0	38.0
66-67	36.500171516806844	38.0	38.0	38.0	34.0	38.0
68-69	36.48465878523231	38.0	38.0	38.0	34.0	38.0
70-71	36.48939994631485	38.0	38.0	38.0	34.0	38.0
72-73	36.471719531787606	38.0	38.0	38.0	34.0	38.0
74-75	36.4629438913326	38.0	38.0	38.0	34.0	38.0
76-77	36.44908039471103	38.0	38.0	38.0	34.0	38.0
78-79	36.433930620347496	38.0	38.0	38.0	34.0	38.0
80-81	36.43347431438507	38.0	38.0	38.0	34.0	38.0
82-83	36.41028912562292	38.0	38.0	38.0	34.0	38.0
84-85	36.40122624192257	38.0	38.0	38.0	34.0	38.0
86-87	36.3853477464088	38.0	38.0	38.0	34.0	38.0
88-89	36.38362469710776	38.0	38.0	38.0	34.0	38.0
90-91	36.39073537046277	38.0	38.0	38.0	34.0	38.0
92-93	36.36331922769686	38.0	38.0	38.0	34.0	38.0
94-95	36.35461890261314	38.0	38.0	38.0	34.0	38.0
96-97	36.34892064188173	38.0	37.0	38.0	34.0	38.0
98-99	36.37143411639368	38.0	37.0	38.0	34.0	38.0
100-101	35.82848963278768	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	9.0
19	28.0
20	107.0
21	448.0
22	1894.0
23	5432.0
24	12286.0
25	23577.0
26	39460.0
27	60842.0
28	87168.0
29	117345.0
30	151278.0
31	193112.0
32	246318.0
33	324727.0
34	458044.0
35	744908.0
36	1802475.0
37	5760620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.078364280245026	18.88712752041858	13.821205812914753	22.213302386421642
2	14.649733601327208	20.099839682235835	41.079472945380296	24.17095377105666
3	19.784523803707906	25.604032155227568	29.383923443048836	25.227520598015683
4	12.922548972690148	18.14590711140888	39.07231049004594	29.85923342585503
5	13.873652557650487	37.97332860391671	34.388092323771794	13.764926514661008
6	30.014586124936194	37.63645952973456	18.62760815467075	13.721346190658497
7	26.706277517228177	33.10912774374681	22.3922540996682	17.792340639356816
8	24.23192038348647	36.930931757274124	20.720143807427256	18.11700405181215
9	25.344613402884125	17.810147077590607	21.267218207631444	35.57802131189383
10-11	24.275838278458398	27.03277541156202	28.281250000000004	20.41013630997958
12-13	25.189974815254455	24.42222915581078	28.64663734719152	21.74115868174325
14-15	22.362173526900495	25.784126660655417	26.528458009690052	25.32524180275404
16-17	21.696491952207758	29.04595476805768	27.170072422154163	22.0874808575804
18-19	21.64607361057938	27.94693063265697	29.179722395035736	21.22727336172792
20-21	22.50368934147093	27.017629725660953	29.220426930201192	21.258254002666924
22-23	22.160875185449058	26.960557797664592	29.150470694998187	21.728096321888167
24-25	21.92512579788328	27.091313620324105	29.26793297593481	21.71562760585781
26-27	21.834630431978205	27.233356931451446	29.212033454508678	21.719979182061667
28-29	21.6648063765186	27.19662222654982	29.301166076687984	21.837405320243594
30-31	21.99367316897951	27.01472973927595	29.234352600638076	21.757244491106466
32-33	21.771508160987153	27.191039485524684	29.20885438553926	21.8285979679489
34-35	21.9486948406195	27.31649653945946	29.160466130641016	21.57434248928002
36-37	21.95395961886362	27.18554371022069	29.03663694393544	21.82385972698025
38-39	21.780674849491884	27.31750374991862	29.129707259627303	21.77211414096219
40-41	22.08976936308972	27.1476178054044	28.99335370202772	21.769259129478165
42-43	22.015283897170253	27.18927389801944	28.87599268884563	21.919449515964672
44-45	22.0338693191147	27.32599472578775	28.753151428869444	21.886984526228108
46-47	22.135210388147115	27.28859448706472	28.417866666790847	22.158328457997317
48-49	22.017118675004422	27.400137327169627	28.46141032522272	22.12133367260323
50-51	21.98783283719415	27.5873899637172	28.363126868916837	22.061650330171812
52-53	22.181134112417386	27.570499392880727	28.260651191437592	21.987715303264295
54-55	22.15529567867557	27.44601025609225	28.23301890859497	22.165675156637207
56-57	22.136493779588985	27.651075099989114	28.15589016620849	22.05654095421341
58-59	22.051490189403182	27.74571493058285	28.26767289716511	21.935121982848862
60-61	22.171774779312813	27.724573384309064	28.219005366831674	21.884646469546446
62-63	22.019286783482322	27.868837355656318	28.13699161547064	21.974884245390722
64-65	22.02355053302362	27.81810863627309	28.094172492074176	22.06416833862911
66-67	22.129210323094675	27.867180431462202	27.9366217606469	22.066987484796226
68-69	22.060506508716816	27.73702306826051	28.08326196356185	22.119208459460825
70-71	22.06574631856258	27.463318383470725	28.179377200064444	22.291558097902247
72-73	22.143974499316542	27.46745770210256	28.09622310829283	22.292344690288076
74-75	21.98006666010234	27.583267551966806	28.10940037967559	22.32726540825527
76-77	22.169713891870266	27.502197681556833	28.019164036610245	22.30892438996265
78-79	22.15362041658506	27.59798772683436	27.913982361825823	22.33440949475476
80-81	22.160859089842944	27.602985657245902	27.900585748934155	22.335569503976995
82-83	22.14341856710767	27.587406434534127	27.993313169911772	22.27586182844643
84-85	22.33241049183682	27.566292357354577	27.92251803797527	22.178779112833336
86-87	22.144082240958845	27.59742639796081	27.966324350806893	22.292167010273445
88-89	22.213732665880233	27.60600013521254	27.93319869530826	22.24706850359897
90-91	22.220811591787516	27.635418479313163	27.943699097822865	22.20007083107646
92-93	22.219507024290618	27.563674554299467	27.99713813050021	22.2196802909097
94-95	22.11661782217522	27.71655012692235	27.94269051882737	22.224141532075066
96-97	22.159391254027856	27.70601024553202	27.96567322547877	22.168925274961353
98-99	22.424173651927358	28.28542191764956	27.94809312007544	21.34231131034764
100-101	23.044121095541197	30.796257396226455	25.35344298187061	20.806178526361734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2791.0
1	2423.0
2	2686.5
3	3538.5
4	4311.0
5	6025.5
6	7927.0
7	10180.0
8	13691.0
9	16207.5
10	15626.0
11	14238.0
12	14938.0
13	18244.5
14	24845.5
15	32761.5
16	39749.5
17	45212.0
18	46775.5
19	43995.0
20	39745.5
21	36065.5
22	34641.0
23	37387.5
24	44556.0
25	56480.5
26	72834.0
27	91231.5
28	111527.5
29	133408.5
30	153987.5
31	177072.0
32	202108.0
33	225609.5
34	249929.5
35	272541.0
36	296817.5
37	320306.0
38	337480.0
39	349749.0
40	364575.5
41	379653.5
42	388195.0
43	397882.5
44	412714.5
45	421540.5
46	420526.0
47	416979.0
48	410679.5
49	401451.0
50	391367.5
51	379955.5
52	362358.5
53	343416.0
54	325705.5
55	304213.0
56	279545.5
57	253006.0
58	226196.0
59	197305.5
60	165437.0
61	134540.0
62	105162.0
63	79747.0
64	59879.0
65	44382.5
66	32935.5
67	25020.0
68	18887.5
69	13760.0
70	9932.0
71	7043.5
72	4933.0
73	3360.0
74	2189.5
75	1424.5
76	882.5
77	537.0
78	351.5
79	218.0
80	144.0
81	95.5
82	63.0
83	39.5
84	24.0
85	18.0
86	18.5
87	15.5
88	7.5
89	4.5
90	4.0
91	3.0
92	2.5
93	2.0
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0010867311128126595
4	0.0
5	0.048683559851965284
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.985005104645227E-6
14-15	3.9880040837161815E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.9865711638557366E-6
28-29	4.98826435985379E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.5151694216992261E-5
40-41	0.0
42-43	0.0
44-45	1.0271303646697967E-5
46-47	2.742288142459903E-4
48-49	0.0
50-51	4.1974532372228476E-5
52-53	1.0557217877487181E-5
54-55	0.0018210210279085012
56-57	0.0024360283463951108
58-59	7.957104753671156E-4
60-61	0.0027177752789644734
62-63	0.004579057592878824
64-65	0.0019979170068568624
66-67	0.007184786173794391
68-69	0.004188040918107795
70-71	0.0017100694288188101
72-73	7.940541227290051E-4
74-75	5.94328947471436E-4
76-77	0.0
78-79	0.0
80-81	2.2265757637814055E-4
82-83	0.0
84-85	9.001990453359317E-4
86-87	9.862198032575683E-4
88-89	1.0923801628617448E-4
90-91	4.7179646675915104E-4
92-93	2.1658280477331173E-4
94-95	2.4998240748807304E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	101.0
20-21	409.0
22-23	853.0
24-25	1305.0
26-27	2694.0
28-29	5892.0
30-31	15097.0
32-33	20090.0
34-35	27381.0
36-37	43479.0
38-39	50281.0
40-41	51149.0
42-43	58673.0
44-45	70713.0
46-47	71958.0
48-49	65463.0
50-51	59174.0
52-53	53504.0
54-55	57233.0
56-57	59292.0
58-59	62888.0
60-61	64731.0
62-63	62125.0
64-65	68278.0
66-67	73281.0
68-69	74023.0
70-71	78020.0
72-73	66218.0
74-75	67466.0
76-77	71354.0
78-79	74746.0
80-81	75180.0
82-83	72286.0
84-85	71692.0
86-87	74951.0
88-89	77917.0
90-91	80682.0
92-93	78719.0
94-95	85599.0
96-97	144510.0
98-99	856815.0
100-101	6933858.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.7971395109977
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71078243903916	73.4499902973489
2	7.582137557649317	12.555586023045318
3	1.9822448848263237	4.9237261882178
4	0.7547156409192245	2.499531848492843
5	0.3563264723941827	1.4751406323141405
6	0.18619693737032667	0.9249944279982862
7	0.12172419777138371	0.7054890769319073
8	0.07960455790188721	0.5272823749051079
9	0.05468758936923292	0.4075178369872079
>10	0.1668119027540991	2.125030165115802
>50	0.003877524679787622	0.21222125541405568
>100	7.383741099802485E-4	0.09375972991452577
>500	1.3021854268631036E-4	0.07403419882568742
>1k	2.1702672406396974E-5	0.025695944488413204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.970010209290454E-6	0.0	0.0	0.0
3	0.0	9.970010209290454E-6	0.0	0.0	0.0
4	0.0	4.9850051046452274E-5	0.0	9.970010209290454E-6	0.0
5	0.0	4.9850051046452274E-5	0.0	9.970010209290454E-6	0.0
6	7.976008167432363E-5	4.9850051046452274E-5	0.0	9.970010209290454E-6	0.0
7	7.976008167432363E-5	5.9820061255742727E-5	0.0	9.970010209290454E-6	0.0
8	7.976008167432363E-5	5.9820061255742727E-5	0.0	9.970010209290454E-6	0.0
9	7.976008167432363E-5	9.970010209290455E-5	0.0	1.9940020418580908E-5	9.970010209290454E-6
10-11	7.976008167432363E-5	9.970010209290455E-5	0.0	1.9940020418580908E-5	9.970010209290454E-6
12-13	7.976008167432363E-5	9.970010209290455E-5	0.0	5.48350561510975E-5	9.970010209290454E-6
14-15	7.976008167432363E-5	1.3459513782542116E-4	0.0	5.9820061255742727E-5	9.970010209290454E-6
16-17	8.973009188361409E-5	1.3958014293006637E-4	0.0	6.480506636038796E-5	9.970010209290454E-6
18-19	8.973009188361409E-5	1.495501531393568E-4	0.0	6.979007146503319E-5	9.970010209290454E-6
20-21	9.471509698825932E-5	1.645051684532925E-4	0.0	7.47750765696784E-5	9.970010209290454E-6
22-23	9.970010209290455E-5	1.8943019397651864E-4	0.0	9.970010209290455E-5	1.4955015313935682E-5
24-25	9.970010209290455E-5	2.1435521949974477E-4	0.0	1.3459513782542113E-4	1.9940020418580908E-5
26-27	9.970010209290455E-5	2.1934022460439E-4	0.0	2.0937021439509956E-4	1.9940020418580908E-5
28-29	9.970010209290455E-5	2.2931023481368045E-4	0.0	6.330956482899438E-4	2.4925025523226134E-5
30-31	9.970010209290455E-5	2.891302960694232E-4	0.0	0.0017148417559979582	2.9910030627871363E-5
32-33	9.970010209290455E-5	3.090703164880041E-4	0.0	0.0038982739918325677	2.9910030627871363E-5
34-35	9.970010209290455E-5	3.838453930576825E-4	0.0	0.006684891845329249	3.9880040837161815E-5
36-37	9.970010209290455E-5	4.636054747320061E-4	0.0	0.011031816296579889	3.9880040837161815E-5
38-39	9.970010209290455E-5	5.03485515569168E-4	0.0	0.01857412901990812	3.9880040837161815E-5
40-41	9.970010209290455E-5	5.982006125574272E-4	0.0	0.038005678917815214	3.9880040837161815E-5
42-43	9.970010209290455E-5	6.330956482899438E-4	0.0	0.04541339650331802	3.9880040837161815E-5
44-45	1.0468510719754978E-4	7.627057810107198E-4	0.0	0.05495469627360898	3.9880040837161815E-5
46-47	1.09670112302195E-4	8.474508677896887E-4	0.0	0.06521882178407351	3.9880040837161815E-5
48-49	1.09670112302195E-4	8.474508677896887E-4	0.0	0.07492961172792241	3.9880040837161815E-5
50-51	1.09670112302195E-4	9.22225944359367E-4	0.0	0.08503920208014293	4.4865045941807045E-5
52-53	1.09670112302195E-4	9.272109494640122E-4	0.0	0.10037307778203164	5.9820061255742727E-5
54-55	1.09670112302195E-4	9.272109494640122E-4	0.0	0.13534288859111793	6.979007146503319E-5
56-57	1.09670112302195E-4	9.272109494640122E-4	0.0	0.173842083014293	6.979007146503319E-5
58-59	1.09670112302195E-4	9.571209800918836E-4	0.0	0.21734123755742726	6.979007146503319E-5
60-61	1.3459513782542116E-4	9.970010209290454E-4	0.0	0.2322015377743747	7.976008167432363E-5
62-63	1.495501531393568E-4	0.0010119560362429811	0.0	0.24080067157988772	8.973009188361409E-5
64-65	1.495501531393568E-4	0.0011365811638591118	0.0	0.24894616992087798	9.471509698825932E-5
66-67	1.5952016334864726E-4	0.0011664911944869831	0.0	0.2542452303471159	9.970010209290455E-5
68-69	1.5952016334864726E-4	0.0011764612046962736	0.0	0.2590557602730985	9.970010209290455E-5
70-71	1.645051684532925E-4	0.0011814462098009189	0.0	0.263602084928535	9.970010209290455E-5
72-73	1.994002041858091E-4	0.001191416220010209	0.0	0.2674704488897397	1.1964012251148545E-4
74-75	1.994002041858091E-4	0.0012213262506380806	0.0	0.27066085215671265	1.1964012251148545E-4
76-77	1.994002041858091E-4	0.0012811463118938232	0.0	0.27339761995916284	1.1964012251148545E-4
78-79	1.994002041858091E-4	0.0013210263527309854	0.0	0.27567576729198573	1.1964012251148545E-4
80-81	1.994002041858091E-4	0.0013309963629402756	0.0	0.2767026783435426	1.1964012251148545E-4
82-83	1.994002041858091E-4	0.0013409663731495662	0.0	0.2770316886804492	1.1964012251148545E-4
84-85	1.994002041858091E-4	0.0013459513782542113	0.0	0.2773307889867279	1.296101327207759E-4
86-87	2.0937021439509956E-4	0.0013559213884635018	0.0	0.27743048908882084	1.296101327207759E-4
88-89	2.2931023481368045E-4	0.001425711459928535	0.0	0.2774952941551812	1.296101327207759E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15050	0.0	52.54787	1
GTATCAA	28060	0.0	39.44524	1
TCAACGC	31830	0.0	34.73203	4
ATCAACG	32025	0.0	34.424885	3
CAACGCA	32995	0.0	33.465904	5
TATCAAC	32935	0.0	33.31426	2
AACGCAG	33930	0.0	32.541824	6
ACGCAGA	39280	0.0	28.0873	7
CGCAGAG	39960	0.0	27.620287	8
GTGGTAT	7030	0.0	27.205679	1
TGGTATC	6635	0.0	26.780495	2
GCAGAGT	46390	0.0	23.75419	9
GAGTACT	27660	0.0	22.02315	12-13
CAGAGTA	44840	0.0	20.985107	10-11
GTACATG	33025	0.0	20.116962	1
GTACTTT	30215	0.0	20.009535	14-15
TACATGG	32285	0.0	19.968037	2
ACATGGG	32705	0.0	19.24324	3
GTGTAGC	6415	0.0	18.69336	1
AGAGTAC	42005	0.0	18.313772	10-11
>>END_MODULE