FastQCFastQC Report
Fri 27 May 2016
SRR2139319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10837917
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA172600.051.9396251
GTATCAA321200.039.780311
CTTATAC89100.037.3866541
TCAACGC375700.033.86944
ATCAACG375900.033.7819753
TATCAAC382500.033.0388532
CAACGCA385600.032.954425
AACGCAG399000.031.9742076
TATACAC139300.028.3442553
ACGCAGA462500.027.5463967
TGGTATC77800.027.1754782
CGCAGAG468750.027.151118
GTGGTAT83900.027.1300771
TTATACA142000.025.4008662
GCAGAGT536800.023.7091719
GAGTACT326400.021.83514612-13
ACACATC169150.021.2869286
TACACAT191000.021.2446695
ACATCTC175600.021.0282488
CAGAGTA528650.020.46638310-11
Produced by FastQC (version 0.11.5)