##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10837917
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.936489087340306	31.0	18.0	33.0	18.0	33.0
2	27.991536473290946	29.0	25.0	32.0	18.0	33.0
3	28.249569728205152	29.0	27.0	31.0	18.0	33.0
4	31.236706555327928	33.0	31.0	33.0	27.0	33.0
5	31.87021537441189	33.0	32.0	33.0	30.0	34.0
6	35.91651384671058	38.0	36.0	38.0	31.0	38.0
7	36.2408139866729	38.0	37.0	38.0	33.0	38.0
8	36.60055553110436	38.0	37.0	38.0	34.0	38.0
9	36.84510279973541	38.0	38.0	38.0	35.0	38.0
10-11	36.892095685914555	38.0	38.0	38.0	35.0	38.0
12-13	36.95189043245118	38.0	38.0	38.0	35.0	38.0
14-15	36.949351891142925	38.0	38.0	38.0	35.0	38.0
16-17	36.963542809932946	38.0	38.0	38.0	35.0	38.0
18-19	36.976264581099855	38.0	38.0	38.0	35.0	38.0
20-21	36.988734119614406	38.0	38.0	38.0	35.5	38.0
22-23	37.004091664283614	38.0	38.0	38.0	36.0	38.0
24-25	36.99340886842076	38.0	38.0	38.0	36.0	38.0
26-27	36.9635565087634	38.0	38.0	38.0	35.5	38.0
28-29	36.942847263390924	38.0	38.0	38.0	35.0	38.0
30-31	36.939018534604315	38.0	38.0	38.0	35.0	38.0
32-33	36.90571195780345	38.0	38.0	38.0	35.0	38.0
34-35	36.875666872884736	38.0	38.0	38.0	35.0	38.0
36-37	36.84369513819762	38.0	38.0	38.0	35.0	38.0
38-39	36.8058303544608	38.0	38.0	38.0	35.0	38.0
40-41	36.824970995523465	38.0	38.0	38.0	35.0	38.0
42-43	36.77240071220427	38.0	38.0	38.0	35.0	38.0
44-45	36.73217856203975	38.0	38.0	38.0	35.0	38.0
46-47	36.68112150149385	38.0	38.0	38.0	34.5	38.0
48-49	36.64133367803759	38.0	38.0	38.0	34.0	38.0
50-51	36.61124693170653	38.0	38.0	38.0	34.0	38.0
52-53	36.59004772648581	38.0	38.0	38.0	34.0	38.0
54-55	36.57465372488214	38.0	38.0	38.0	34.0	38.0
56-57	36.55732632502277	38.0	38.0	38.0	34.0	38.0
58-59	36.5432970138666	38.0	38.0	38.0	34.0	38.0
60-61	36.54537715109846	38.0	38.0	38.0	34.0	38.0
62-63	36.53457715547113	38.0	38.0	38.0	34.0	38.0
64-65	36.527979756847124	38.0	38.0	38.0	34.0	38.0
66-67	36.49646296603302	38.0	38.0	38.0	34.0	38.0
68-69	36.47908025456546	38.0	38.0	38.0	34.0	38.0
70-71	36.48293972254816	38.0	38.0	38.0	34.0	38.0
72-73	36.466263178996115	38.0	38.0	38.0	34.0	38.0
74-75	36.460007508086775	38.0	38.0	38.0	34.0	38.0
76-77	36.45065630281738	38.0	38.0	38.0	34.0	38.0
78-79	36.437959471074386	38.0	38.0	38.0	34.0	38.0
80-81	36.437356731565785	38.0	38.0	38.0	34.0	38.0
82-83	36.41869102733605	38.0	38.0	38.0	34.0	38.0
84-85	36.411945401789964	38.0	38.0	38.0	34.0	38.0
86-87	36.39934166791129	38.0	38.0	38.0	34.0	38.0
88-89	36.40153145689295	38.0	38.0	38.0	34.0	38.0
90-91	36.40630701397338	38.0	38.0	38.0	34.0	38.0
92-93	36.377615851004435	38.0	38.0	38.0	34.0	38.0
94-95	36.374196209627215	38.0	38.0	38.0	34.0	38.0
96-97	36.372947222092435	38.0	38.0	38.0	34.0	38.0
98-99	36.40079674404718	38.0	38.0	38.0	34.0	38.0
100-101	35.86021168798905	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	25.0
19	114.0
20	247.0
21	688.0
22	2185.0
23	6014.0
24	13552.0
25	25858.0
26	43085.0
27	66595.0
28	96579.0
29	129949.0
30	166434.0
31	211433.0
32	269175.0
33	351496.0
34	494659.0
35	806262.0
36	1962516.0
37	6191050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.10423912639301	18.96824823441626	13.795150857863186	22.132361781327536
2	14.528834276918712	20.07976255953981	41.38498200346063	24.006421160080855
3	19.61105050024876	25.885105179940442	29.64001576702595	24.863828552784845
4	12.718689393912133	18.4875100999574	39.18005646287936	29.613744043251117
5	13.62559780929497	38.14719113290279	34.62889182424622	13.598319233556028
6	29.58131161181618	37.81543076958423	18.781847102169174	13.821410516430419
7	26.338935793658504	33.357037150219924	22.41758264064949	17.886444415472088
8	23.9915197726648	37.044738393918315	20.894024193025285	18.069717640391598
9	25.237589474065913	17.77634023216823	21.226154435395657	35.7599158583702
10-11	24.037234276660357	27.07057546205604	28.492758340924734	20.399431920358865
12-13	25.099805852905398	24.46281533909216	28.783465899557353	21.653912908445086
14-15	22.235312072046337	25.863768618204574	26.613663847120396	25.287255462628693
16-17	21.533058428109385	29.21878807523623	27.241438553183233	22.00671494347115
18-19	21.46848421149562	27.976243036369443	29.420445829212383	21.13482692292255
20-21	22.27038627144055	26.95974068735898	29.52464162577979	21.245231415420676
22-23	21.92164037075933	27.1277511137424	29.370613625716302	21.579994889781972
24-25	21.736130577432817	27.302554973469277	29.51862180057304	21.442692648524865
26-27	21.78538298379687	27.354014919395087	29.491221980907977	21.36938011590006
28-29	21.565884165920757	27.24477480707777	29.652291497212396	21.537049529789073
30-31	22.01363456299623	27.206609296012374	29.423043568375967	21.35671257261542
32-33	21.711052725322528	27.18160069312673	29.492166576792737	21.615180004758002
34-35	21.675412675355872	27.402928114091885	29.64301306626571	21.278646144286537
36-37	21.807987424566374	27.289660281708166	29.359954670986976	21.54239762273848
38-39	21.51745295162307	27.295907997815256	29.554225418248027	21.632413632313646
40-41	21.901496273404632	27.111798424607592	29.422544897030704	21.56416040495707
42-43	21.864174082128326	27.200847087506506	29.246648762686156	21.68833006767901
44-45	21.79976423269246	27.39464204852026	29.099919803774565	21.70567391501271
46-47	22.01384963303416	27.257078124703167	28.67949097519032	22.04958126707236
48-49	21.86270229744058	27.373678172837085	28.69146479762194	22.07215473210039
50-51	21.780157064976517	27.55677430952033	28.712858509311555	21.9502101161916
52-53	21.97027390665551	27.505147975500556	28.7276359801515	21.796942137692437
54-55	21.906647079661585	27.499694648513927	28.568326666001635	22.025331605822846
56-57	22.07404056461557	27.769089395028075	28.271372869964484	21.885497170391876
58-59	21.800153144610093	28.012915374428232	28.570776583968378	21.616154896993297
60-61	22.0134309606951	28.193266382555514	28.22747362801545	21.565829028733933
62-63	21.782740178508185	28.400612539342944	28.161061009390114	21.655586272758757
64-65	21.74827011550283	28.35055651419308	28.166538012314092	21.734635357989994
66-67	21.829748686238055	28.280844999694903	28.0837174127118	21.805688901355243
68-69	21.86680896224772	28.107220201981626	28.173740633791606	21.852230201979047
70-71	21.855550641180095	27.729955253708223	28.37664317771206	22.03785092739962
72-73	21.969401701707344	27.629780846074826	28.357072274184713	22.043745178033117
74-75	21.871141458140006	27.7375683352348	28.318528060935762	22.07276214568943
76-77	21.992456567631546	27.68454620939322	28.238403626994984	22.08459359598025
78-79	21.949562368041203	27.690609181620925	28.2083954843302	22.151432966007672
80-81	22.005251675514938	27.684800303096452	28.233690663683152	22.076257357705458
82-83	22.020298544825653	27.767110834326147	28.258616892346467	21.953973728501737
84-85	22.139688758725327	27.702344306420056	28.186914863099826	21.971052071754787
86-87	22.007361033134327	27.758827271686666	28.192244083902786	22.041567611276225
88-89	22.06213439865435	27.743903225983846	28.13747861930392	22.056483756057887
90-91	22.098068887920647	27.828804251943346	28.09854928293879	21.97457757719722
92-93	22.117708006333295	27.72346789581514	28.168250847841414	21.99057325001015
94-95	21.98400464825543	27.854251512348633	28.118192655846485	22.043551183549454
96-97	22.063054279919395	27.855363002514917	28.089829385682663	21.991753331883025
98-99	22.292240307939267	28.405654453019764	28.148984278733842	21.153120960307138
100-101	22.884123306353555	30.98286141969058	25.521568602261652	20.61144667169422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2275.0
1	2131.5
2	2840.0
3	4057.5
4	5086.5
5	7217.5
6	9603.5
7	12450.5
8	16842.5
9	19699.0
10	18708.0
11	17212.5
12	18230.0
13	22191.5
14	30271.5
15	40002.5
16	48243.5
17	54621.0
18	56491.0
19	53059.5
20	47706.0
21	42490.5
22	40209.0
23	43475.5
24	51653.0
25	64773.0
26	81596.5
27	101043.0
28	123432.0
29	147831.0
30	173142.5
31	198907.5
32	225018.0
33	251172.5
34	276128.0
35	298893.5
36	323316.5
37	346328.5
38	364733.0
39	380424.0
40	396179.5
41	411111.5
42	420995.0
43	431481.0
44	457470.5
45	473512.0
46	463916.5
47	451638.0
48	439959.0
49	429375.5
50	420617.0
51	407141.5
52	386709.5
53	364043.5
54	340871.5
55	316134.5
56	290093.5
57	260510.5
58	229718.5
59	198161.0
60	163229.5
61	131703.0
62	102967.0
63	77870.0
64	58724.5
65	43648.0
66	32069.0
67	23939.5
68	17823.5
69	12938.5
70	9490.0
71	6976.5
72	4928.5
73	3276.5
74	2047.0
75	1259.5
76	785.0
77	457.0
78	282.0
79	184.5
80	126.5
81	82.5
82	61.5
83	46.0
84	36.5
85	26.0
86	15.5
87	12.0
88	8.5
89	6.0
90	4.5
91	3.0
92	2.5
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0010057283147674964
4	0.0
5	0.0483487740310246
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.613432636548149E-6
14-15	2.7680595819288888E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.614921277288375E-6
28-29	4.616496125605627E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.344496642802691E-6
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.871155600411513E-4
48-49	0.0
50-51	5.3368974751914324E-5
52-53	3.4163648267671204E-5
54-55	0.0017820034441266566
56-57	0.0024450915182936558
58-59	8.203042911310639E-4
60-61	0.0026924656253964566
62-63	0.004634738757408404
64-65	0.0022267579099741557
66-67	0.007339127851800966
68-69	0.0044895405316493255
70-71	0.0020269650970679196
72-73	8.518560312826774E-4
74-75	7.202956813772053E-4
76-77	0.0
78-79	0.0
80-81	1.6816964215724328E-4
82-83	0.0
84-85	8.328349193231159E-4
86-87	9.622245653039286E-4
88-89	1.01004432972336E-4
90-91	4.021210697054786E-4
92-93	2.1156679103553766E-4
94-95	2.3093473547667327E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	125.0
20-21	427.0
22-23	1080.0
24-25	1337.0
26-27	2920.0
28-29	6348.0
30-31	15997.0
32-33	20794.0
34-35	28603.0
36-37	45185.0
38-39	54486.0
40-41	54090.0
42-43	62116.0
44-45	75747.0
46-47	77678.0
48-49	69608.0
50-51	62153.0
52-53	58852.0
54-55	61857.0
56-57	64907.0
58-59	65751.0
60-61	66337.0
62-63	66249.0
64-65	79871.0
66-67	85907.0
68-69	84023.0
70-71	99449.0
72-73	68516.0
74-75	69436.0
76-77	74279.0
78-79	77236.0
80-81	77615.0
82-83	75069.0
84-85	75036.0
86-87	78155.0
88-89	81660.0
90-91	84306.0
92-93	82898.0
94-95	90077.0
96-97	154537.0
98-99	920243.0
100-101	7516957.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.28901912723532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02534694997071	72.43519458847665
2	8.129108353247751	13.378727055355451
3	2.0676533768686047	5.1043550483289994
4	0.8036845365799529	2.64537648811564
5	0.3594257755229083	1.4788397258412995
6	0.2086833586991168	1.03034093373164
7	0.11397431783565631	0.6565184374274345
8	0.07885179820019066	0.5190909704249911
9	0.04792660361159938	0.35494498811684944
>10	0.16097872948613054	2.042169521769174
>50	0.003245683771806405	0.17707706305229365
>100	0.0010799539409739936	0.14618985997885875
>500	2.0281146284088925E-5	0.013251190137879591
>1k	2.02811183680726E-5	0.01792412924273142
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.768059581928889E-5	0.0	9.226865273096298E-6	0.0
3	0.0	2.768059581928889E-5	0.0	9.226865273096298E-6	0.0
4	0.0	3.690746109238519E-5	0.0	9.226865273096298E-6	0.0
5	0.0	3.690746109238519E-5	0.0	9.226865273096298E-6	0.0
6	0.0	3.690746109238519E-5	0.0	9.226865273096298E-6	1.8453730546192595E-5
7	0.0	3.690746109238519E-5	0.0	2.768059581928889E-5	1.8453730546192595E-5
8	0.0	3.690746109238519E-5	0.0	2.768059581928889E-5	2.768059581928889E-5
9	0.0	1.7531044018882964E-4	0.0	5.536119163857778E-5	2.768059581928889E-5
10-11	0.0	1.7531044018882964E-4	0.0	1.1994924855025186E-4	2.768059581928889E-5
12-13	0.0	1.7531044018882964E-4	0.0	1.337895464598963E-4	2.768059581928889E-5
14-15	0.0	2.8141939082943705E-4	0.0	1.4762984436954076E-4	3.690746109238519E-5
16-17	0.0	2.9525968873908153E-4	0.0	1.4762984436954076E-4	3.690746109238519E-5
18-19	4.613432636548149E-6	3.2294028455837043E-4	0.0	1.5685670964263706E-4	3.690746109238519E-5
20-21	9.226865273096298E-6	3.644611782873037E-4	0.0	1.5685670964263706E-4	3.690746109238519E-5
22-23	9.226865273096298E-6	4.4750296574517037E-4	0.0	1.891507380984741E-4	5.074775900202964E-5
24-25	9.226865273096298E-6	5.074775900202964E-4	0.0	2.3067163182740742E-4	6.458805691167408E-5
26-27	9.226865273096298E-6	5.443850511126816E-4	0.0	2.3528506446395556E-4	6.458805691167408E-5
28-29	9.226865273096298E-6	5.443850511126816E-4	0.0	4.2904923519897784E-4	6.458805691167408E-5
30-31	9.226865273096298E-6	6.458805691167408E-4	0.0	9.088462293999853E-4	6.458805691167408E-5
32-33	9.226865273096298E-6	6.458805691167408E-4	0.0	0.0019791626010791557	9.688208536751111E-5
34-35	9.226865273096298E-6	7.519895197573482E-4	0.0	0.0034693013426842076	1.0149551800405927E-4
36-37	9.226865273096298E-6	8.580984703979556E-4	0.0	0.0058775131789623415	1.0149551800405927E-4
38-39	9.226865273096298E-6	8.99619364126889E-4	0.0	0.009605166749293246	1.0149551800405927E-4
40-41	9.226865273096298E-6	0.0010103417474040446	0.0	0.01342970240499166	1.0149551800405927E-4
42-43	9.226865273096298E-6	0.0010380223432233334	0.0	0.017148129110049468	1.0149551800405927E-4
44-45	9.226865273096298E-6	0.0011210641306812	0.0	0.021461688625221985	1.0149551800405927E-4
46-47	9.226865273096298E-6	0.0011810387549563261	0.0	0.026098188424952876	1.0149551800405927E-4
48-49	9.226865273096298E-6	0.001204105918139067	0.0	0.030734688224683768	1.0149551800405927E-4
50-51	9.226865273096298E-6	0.0014024835215106371	0.0	0.03568028801106338	1.1072238327715557E-4
52-53	9.226865273096298E-6	0.0014117103867837333	0.0	0.03992925946932423	1.1072238327715557E-4
54-55	9.226865273096298E-6	0.001430164117329926	0.0	0.04445503688577796	1.1072238327715557E-4
56-57	9.226865273096298E-6	0.0014670715784223113	0.0	0.0492114859340591	1.1072238327715557E-4
58-59	9.226865273096298E-6	0.0014809118763319557	0.0	0.054106337961436685	1.1072238327715557E-4
60-61	9.226865273096298E-6	0.0015085924721512445	0.0	0.05864595567580007	1.1072238327715557E-4
62-63	9.226865273096298E-6	0.0015501133658801778	0.0	0.06285801967296853	1.1072238327715557E-4
64-65	9.226865273096298E-6	0.0017346506713421038	0.0	0.06699165531531567	1.1533581591370371E-4
66-67	9.226865273096298E-6	0.0017531044018882964	0.0	0.07161431481713691	1.245626811868E-4
68-69	9.226865273096298E-6	0.001799238728253778	0.0	0.07620006685786576	1.2917611382334815E-4
70-71	9.226865273096298E-6	0.0018269193240730668	0.0	0.08066125621740783	1.2917611382334815E-4
72-73	9.226865273096298E-6	0.0018545999198923556	0.0	0.08393679338935701	1.5685670964263706E-4
74-75	9.226865273096298E-6	0.0019145745441674817	0.0	0.08652031566582397	1.5685670964263706E-4
76-77	9.226865273096298E-6	0.002020683494808089	0.0	0.08905309018328891	1.5685670964263706E-4
78-79	9.226865273096298E-6	0.0020622043885370226	0.0	0.09114297516764522	1.6147014227918522E-4
80-81	9.226865273096298E-6	0.0020668178211735704	0.0	0.09210256915604724	1.8453730546192596E-4
82-83	9.226865273096298E-6	0.002076044686446667	0.0	0.0924808706322442	2.076044686446667E-4
84-85	9.226865273096298E-6	0.002112952147539052	0.0	0.09278074375361982	2.1221790128121484E-4
86-87	1.8453730546192595E-5	0.002140632743358341	0.0	0.09294221389589899	2.1221790128121484E-4
88-89	1.8453730546192595E-5	0.0022790357224547852	0.0	0.09301602881808377	2.2144476655431113E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17260	0.0	51.939625	1
GTATCAA	32120	0.0	39.78031	1
CTTATAC	8910	0.0	37.386654	1
TCAACGC	37570	0.0	33.8694	4
ATCAACG	37590	0.0	33.781975	3
TATCAAC	38250	0.0	33.038853	2
CAACGCA	38560	0.0	32.95442	5
AACGCAG	39900	0.0	31.974207	6
TATACAC	13930	0.0	28.344255	3
ACGCAGA	46250	0.0	27.546396	7
TGGTATC	7780	0.0	27.175478	2
CGCAGAG	46875	0.0	27.15111	8
GTGGTAT	8390	0.0	27.130077	1
TTATACA	14200	0.0	25.400866	2
GCAGAGT	53680	0.0	23.709171	9
GAGTACT	32640	0.0	21.835146	12-13
ACACATC	16915	0.0	21.286928	6
TACACAT	19100	0.0	21.244669	5
ACATCTC	17560	0.0	21.028248	8
CAGAGTA	52865	0.0	20.466383	10-11
>>END_MODULE