FastQCFastQC Report
Fri 27 May 2016
SRR2139318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10111957
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA162150.050.9043051
GTATCAA289700.038.7807771
TCAACGC338700.033.0530054
ATCAACG341800.032.6501853
TATCAAC346300.032.1240542
CTTATAC78600.031.9755421
CAACGCA350350.031.9664975
AACGCAG359850.031.1688926
ACGCAGA413600.027.0650187
CGCAGAG419000.026.716348
TATACAC122900.024.6473623
GCAGAGT485250.023.0688239
GTGGTAT83300.022.8138141
TGGTATC79950.022.004932
GAGTACT289600.021.82266812-13
TTATACA124400.021.6209622
CAGAGTA471950.020.4365610-11
GTACTTT319700.019.61014714-15
ACATCTC141050.018.9346288
GTACATG325450.018.7100521
Produced by FastQC (version 0.11.5)