##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10111957
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.11899575917896	31.0	18.0	33.0	18.0	33.0
2	28.12107952990702	29.0	27.0	33.0	18.0	33.0
3	28.355715812478238	30.0	27.0	31.0	18.0	33.0
4	31.275410585705615	33.0	31.0	33.0	28.0	33.0
5	31.893304728253888	33.0	32.0	33.0	30.0	34.0
6	35.93724152505791	38.0	36.0	38.0	31.0	38.0
7	36.25507159494448	38.0	37.0	38.0	33.0	38.0
8	36.60412875568992	38.0	37.0	38.0	34.0	38.0
9	36.848792968561874	38.0	38.0	38.0	35.0	38.0
10-11	36.895224880802004	38.0	38.0	38.0	35.0	38.0
12-13	36.95533500587473	38.0	38.0	38.0	35.0	38.0
14-15	36.94894934778698	38.0	38.0	38.0	35.0	38.0
16-17	36.96471345754338	38.0	38.0	38.0	35.0	38.0
18-19	36.97555478133461	38.0	38.0	38.0	35.0	38.0
20-21	36.98559861335569	38.0	38.0	38.0	35.5	38.0
22-23	37.00028164973829	38.0	38.0	38.0	36.0	38.0
24-25	36.987234366555505	38.0	38.0	38.0	36.0	38.0
26-27	36.956657883871635	38.0	38.0	38.0	35.5	38.0
28-29	36.93428339175905	38.0	38.0	38.0	35.0	38.0
30-31	36.93154970319749	38.0	38.0	38.0	35.0	38.0
32-33	36.905896654477885	38.0	38.0	38.0	35.0	38.0
34-35	36.88117424881354	38.0	38.0	38.0	35.0	38.0
36-37	36.852016273928626	38.0	38.0	38.0	35.0	38.0
38-39	36.82257728652705	38.0	38.0	38.0	35.0	38.0
40-41	36.827518260840236	38.0	38.0	38.0	35.0	38.0
42-43	36.77301594538526	38.0	38.0	38.0	35.0	38.0
44-45	36.73182806602678	38.0	38.0	38.0	35.0	38.0
46-47	36.68408732379483	38.0	38.0	38.0	34.5	38.0
48-49	36.644394555952104	38.0	38.0	38.0	34.0	38.0
50-51	36.61405091848632	38.0	38.0	38.0	34.0	38.0
52-53	36.591862531697416	38.0	38.0	38.0	34.0	38.0
54-55	36.57629486364017	38.0	38.0	38.0	34.0	38.0
56-57	36.55610109715683	38.0	38.0	38.0	34.0	38.0
58-59	36.54155908164885	38.0	38.0	38.0	34.0	38.0
60-61	36.540827551213546	38.0	38.0	38.0	34.0	38.0
62-63	36.531622972209625	38.0	38.0	38.0	34.0	38.0
64-65	36.52035061361488	38.0	38.0	38.0	34.0	38.0
66-67	36.49181390380758	38.0	38.0	38.0	34.0	38.0
68-69	36.476357030597576	38.0	38.0	38.0	34.0	38.0
70-71	36.48068359563901	38.0	38.0	38.0	34.0	38.0
72-73	36.46205686658698	38.0	38.0	38.0	34.0	38.0
74-75	36.45516374781572	38.0	38.0	38.0	34.0	38.0
76-77	36.44220884215355	38.0	38.0	38.0	34.0	38.0
78-79	36.43109289934269	38.0	38.0	38.0	34.0	38.0
80-81	36.42855282996419	38.0	38.0	38.0	34.0	38.0
82-83	36.40818625621438	38.0	38.0	38.0	34.0	38.0
84-85	36.402347460484584	38.0	38.0	38.0	34.0	38.0
86-87	36.386275849068305	38.0	38.0	38.0	34.0	38.0
88-89	36.38630384101996	38.0	38.0	38.0	34.0	38.0
90-91	36.391345184158396	38.0	38.0	38.0	34.0	38.0
92-93	36.360644449831774	38.0	38.0	38.0	34.0	38.0
94-95	36.355267023404565	38.0	38.0	38.0	34.0	38.0
96-97	36.351640138913936	38.0	37.5	38.0	34.0	38.0
98-99	36.382912937764765	38.0	37.5	38.0	34.0	38.0
100-101	35.84396656447052	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	16.0
19	42.0
20	164.0
21	535.0
22	1852.0
23	5458.0
24	12633.0
25	23708.0
26	40244.0
27	62200.0
28	89171.0
29	119691.0
30	156651.0
31	197390.0
32	252162.0
33	330965.0
34	463494.0
35	752565.0
36	1804375.0
37	5798641.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.173659262989354	18.773823899765397	13.76209372725774	22.29042310998751
2	14.601515809452117	20.034094290551273	41.18383810374194	24.180551796254672
3	19.749476184038674	25.55366459540106	29.47823707484672	25.21862214571355
4	12.845139669798833	18.30798924481186	38.97799407177068	29.86887701361863
5	13.818354983511922	37.96693756475092	34.53183284177628	13.68287460996088
6	29.972239794927923	37.58513806971291	18.61987743816553	13.82274469719363
7	26.661179433417292	33.123983814409016	22.249026573194485	17.965810178979204
8	24.203138917620002	36.88982261297195	20.759225934208384	18.147812535199666
9	25.328489826450014	17.6749466003465	21.091100367614303	35.905463205589186
10-11	24.24607818249227	26.93702119184249	28.354046600475062	20.46285402519018
12-13	25.2187731810964	24.32269539912007	28.628489025418126	21.830042394365403
14-15	22.331543323301148	25.7274298455358	26.46897293693321	25.472053894229845
16-17	21.70998155945481	29.027121060740267	27.120487161881723	22.142410217923196
18-19	21.70208496733125	27.83675800836574	29.167598319494438	21.293558704808575
20-21	22.503135701325576	26.92364632789917	29.19475150401418	21.37846646676107
22-23	22.153023410811244	26.977369511218964	29.09427853031753	21.77532854765226
24-25	22.074277842222788	27.082425014757295	29.215491674988247	21.627805468031667
26-27	21.947644396503968	27.238636471552795	29.164961886793648	21.64875724514959
28-29	21.779159347275208	27.14754705718902	29.27043940889663	21.802854186639138
30-31	22.0668848084842	27.054640821594887	29.238292108411766	21.64018226150914
32-33	21.656988484908318	27.059342319339187	29.386355491805137	21.897313703947358
34-35	21.840368344741552	27.15726667597071	29.183171697516165	21.81919328177157
36-37	21.894940433262907	27.145258239610666	29.24132780401357	21.71847352311286
38-39	21.737156234126633	27.199978267261354	29.2629875001232	21.799877998488814
40-41	22.043821044716704	27.083854378184068	29.16114459648008	21.711179980619143
42-43	22.03753217706414	27.094730599990918	28.98729825685927	21.880438966085674
44-45	21.992058977795555	27.316327035448218	28.821365502695212	21.870248484061015
46-47	22.161862554931265	27.211800346208626	28.46181006312518	22.16452703573493
48-49	22.040194392974488	27.328669915953775	28.458100161177285	22.173035529894456
50-51	21.9640733647193	27.456390348210675	28.48205105417387	22.097485232896155
52-53	22.151930436854496	27.408093019119367	28.492536087094933	21.947440456931204
54-55	22.090145128814427	27.353568054931344	28.360483153418077	22.19580366283616
56-57	22.21291485961821	27.606682097949836	28.1190128414582	22.061390200973758
58-59	21.99457592356862	27.820343431654383	28.38062589618986	21.804454748587144
60-61	22.181024597341732	27.949407619239985	28.11100038905415	21.758567394364125
62-63	21.97428959305742	28.152650739065834	28.047590019724606	21.825469648152144
64-65	21.95928377207809	28.09855505620897	28.0123264437592	21.92983472795374
66-67	22.045745816523777	28.024410265622183	27.93754802714494	21.9922958907091
68-69	22.010392950784404	27.894406472883915	28.072695815755566	22.02250476057612
70-71	22.057554458780388	27.502621084615136	28.225435466561894	22.214388990042583
72-73	22.173622988746214	27.435543621352927	28.187971481063972	22.202861908836883
74-75	22.019096247310912	27.609602144806587	28.14130669693703	22.229994910945468
76-77	22.140023982819343	27.530305290937445	28.079222697720958	22.250448028522253
78-79	22.13142665288394	27.614645720536647	27.994371338630923	22.25955628794849
80-81	22.15894327936306	27.601219155688444	27.981884815243752	22.257952749704746
82-83	22.195674755679466	27.58700886986662	28.08661537697668	22.13070099747723
84-85	22.315133186465467	27.552057694565697	27.993608290630824	22.13920082833802
86-87	22.13460581603732	27.56414103055948	28.0943395552606	22.20691359814261
88-89	22.23297489516439	27.59878885480138	27.973184887430964	22.195051362603266
90-91	22.25846233906347	27.64899324169829	27.970250331761328	22.122294087476913
92-93	22.279975776846815	27.61270763378132	27.969973327995774	22.137343261376095
94-95	22.124103290166644	27.744184526560893	27.95272402140807	22.178988161864396
96-97	22.214369006162585	27.704260833586712	27.98393422169082	22.09743593855988
98-99	22.473802279658184	28.287079353513366	27.995969067704817	21.24314929912363
100-101	23.07749000225028	30.834806930127566	25.343258586557415	20.74444448106474
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2041.0
1	1848.5
2	2418.0
3	3489.5
4	4360.0
5	6207.5
6	8344.0
7	10850.0
8	14638.0
9	17236.5
10	16529.0
11	15107.0
12	16007.5
13	19516.0
14	26516.0
15	35047.0
16	42406.0
17	48039.0
18	49344.0
19	46225.0
20	41480.0
21	36690.0
22	34658.5
23	37369.5
24	44415.0
25	56236.0
26	72500.5
27	90968.5
28	110937.0
29	132693.5
30	152943.0
31	174824.0
32	199095.5
33	222476.5
34	246487.0
35	269184.5
36	293117.0
37	314297.0
38	329566.5
39	342898.5
40	358188.5
41	373159.5
42	384356.5
43	393216.0
44	402384.0
45	419177.5
46	427063.0
47	423048.5
48	418613.5
49	407905.5
50	394695.0
51	381190.5
52	362769.5
53	342018.0
54	322803.0
55	301254.0
56	277092.5
57	251715.0
58	225062.5
59	195203.5
60	162851.0
61	132304.0
62	103575.5
63	78671.5
64	59132.0
65	43780.5
66	32320.5
67	24349.5
68	18317.5
69	13428.5
70	9694.0
71	7042.5
72	4915.0
73	3277.5
74	2178.0
75	1397.5
76	845.0
77	467.0
78	279.5
79	180.0
80	118.0
81	83.0
82	62.5
83	40.5
84	23.5
85	17.5
86	15.0
87	11.0
88	7.0
89	4.5
90	4.5
91	3.5
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0010284853861621446
4	0.0
5	0.04903106292876839
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.4723206398128474E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.4838507331236586E-5
28-29	9.895526005095999E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	3.5020515017697367E-5
40-41	0.0
42-43	0.0
44-45	5.079458218891023E-6
46-47	2.8639250993577444E-4
48-49	0.0
50-51	3.625321190509903E-5
52-53	5.206884355046406E-6
54-55	0.0017482949413347596
56-57	0.0023898054898359045
58-59	8.100786491064912E-4
60-61	0.0025353926971741878
62-63	0.0045059202219591655
64-65	0.0019190295661543127
66-67	0.006982810611678463
68-69	0.004153855297632215
70-71	0.0016835155292716157
72-73	8.123401818061839E-4
74-75	6.72039661092639E-4
76-77	0.0
78-79	5.680008324620201E-6
80-81	1.2018476175116522E-4
82-83	0.0
84-85	8.191750639857062E-4
86-87	8.840076574967948E-4
88-89	8.262200703254918E-5
90-91	4.106455759098879E-4
92-93	1.9811699997550553E-4
94-95	3.028631225866352E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	106.0
20-21	440.0
22-23	936.0
24-25	1193.0
26-27	2580.0
28-29	5403.0
30-31	13705.0
32-33	18360.0
34-35	24746.0
36-37	38727.0
38-39	46372.0
40-41	46511.0
42-43	53562.0
44-45	65344.0
46-47	66443.0
48-49	59796.0
50-51	52721.0
52-53	49882.0
54-55	52873.0
56-57	54885.0
58-59	55961.0
60-61	55393.0
62-63	55509.0
64-65	66379.0
66-67	71345.0
68-69	69594.0
70-71	82222.0
72-73	58362.0
74-75	59761.0
76-77	63547.0
78-79	65867.0
80-81	66666.0
82-83	64543.0
84-85	65027.0
86-87	67624.0
88-89	70078.0
90-91	73009.0
92-93	73070.0
94-95	80074.0
96-97	141706.0
98-99	877633.0
100-101	7174002.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.49239115081296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33071654509371	72.86612019869456
2	7.91019790035667	13.050622785531237
3	2.01853834291165	4.99542163609145
4	0.7612087678590636	2.5117572570263302
5	0.3596304978227851	1.4833389848079388
6	0.20215684045363094	1.0005840693908046
7	0.11565307523974902	0.6678349104330245
8	0.07797653173064108	0.5145976444886258
9	0.05115963205658444	0.37982523408690894
>10	0.16838756037340588	2.1518685490454565
>50	0.0033899509021707145	0.18768675582145758
>100	8.973774098428592E-4	0.12854748496493879
>500	6.523360138656146E-5	0.03842218608062913
>1k	2.174418877402059E-5	0.0233723035366152
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.889282559251389E-6	0.0	9.889282559251389E-6	0.0
3	0.0	1.9778565118502777E-5	0.0	9.889282559251389E-6	0.0
4	0.0	7.911426047401111E-5	0.0	9.889282559251389E-6	0.0
5	0.0	8.900354303326251E-5	0.0	9.889282559251389E-6	0.0
6	0.0	1.0878210815176528E-4	0.0	9.889282559251389E-6	0.0
7	0.0	1.0878210815176528E-4	0.0	9.889282559251389E-6	0.0
8	0.0	1.1867139071101667E-4	0.0	9.889282559251389E-6	0.0
9	0.0	1.977856511850278E-4	0.0	9.889282559251389E-6	9.889282559251389E-6
10-11	0.0	2.027302924646535E-4	0.0	2.9667847677754168E-5	9.889282559251389E-6
12-13	0.0	2.0767493374427917E-4	0.0	2.9667847677754168E-5	9.889282559251389E-6
14-15	0.0	2.670106290997875E-4	0.0	2.9667847677754168E-5	1.4833923838877084E-5
16-17	0.0	2.670106290997875E-4	0.0	2.9667847677754168E-5	1.9778565118502777E-5
18-19	0.0	2.867891942182903E-4	0.0	2.9667847677754168E-5	1.9778565118502777E-5
20-21	0.0	3.2140168317567014E-4	0.0	2.9667847677754168E-5	1.9778565118502777E-5
22-23	0.0	3.3623560701454724E-4	0.0	3.461248895737986E-5	2.9667847677754168E-5
24-25	0.0	3.659034546923014E-4	0.0	3.9557130237005555E-5	3.9557130237005555E-5
26-27	0.0	3.659034546923014E-4	0.0	6.922497791475972E-5	3.9557130237005555E-5
28-29	0.0	3.659034546923014E-4	0.0	2.1756421630353054E-4	3.9557130237005555E-5
30-31	0.0	4.4007307388668683E-4	0.0	7.515854745031056E-4	3.9557130237005555E-5
32-33	0.0	4.549069977255639E-4	0.0	0.0016960119589116132	5.9335695355508335E-5
34-35	0.0	5.34021258199575E-4	0.0	0.002754165192751512	5.9335695355508335E-5
36-37	0.0	6.180801599532118E-4	0.0	0.004796302041236924	5.9335695355508335E-5
38-39	0.0	6.329140837920889E-4	0.0	0.007713640396216083	5.9335695355508335E-5
40-41	0.0	7.219176268253514E-4	0.0	0.010873266173896903	5.9335695355508335E-5
42-43	0.0	7.318069093846028E-4	0.0	0.01384994022423157	5.9335695355508335E-5
44-45	0.0	7.812533221808597E-4	0.0	0.017449639075799078	5.9335695355508335E-5
46-47	0.0	8.306997349771167E-4	0.0	0.021509189566371774	5.9335695355508335E-5
48-49	0.0	8.504783000956195E-4	0.0	0.025415456177276072	5.9335695355508335E-5
50-51	0.0	0.0010284853861621444	0.0	0.02921788532130823	5.9335695355508335E-5
52-53	0.0	0.0010383746687213959	0.0	0.033049982313018145	6.428033663513402E-5
54-55	0.0	0.0010581532338398984	0.0	0.03706008639079458	6.922497791475972E-5
56-57	0.0	0.00106804251639915	0.0	0.041188861859282035	6.922497791475972E-5
58-59	0.0	0.0010779317989584015	0.0	0.044941844590517935	6.922497791475972E-5
60-61	0.0	0.0011273782117546584	4.944641279625694E-6	0.049021173646209136	6.922497791475972E-5
62-63	0.0	0.0011570460594324127	9.889282559251389E-6	0.05321917409261135	6.922497791475972E-5
64-65	0.0	0.0012658281675841777	9.889282559251389E-6	0.05672986940114559	6.922497791475972E-5
66-67	0.0	0.0012757174501434293	9.889282559251389E-6	0.06019606293816321	6.922497791475972E-5
68-69	0.0	0.0013053852978211833	9.889282559251389E-6	0.06410727419034713	6.922497791475972E-5
70-71	0.0	0.0013399977867785632	9.889282559251389E-6	0.06778114266110902	7.911426047401111E-5
72-73	0.0	0.0013548317106174402	9.889282559251389E-6	0.07073803814632518	9.88928255925139E-5
74-75	0.0	0.0013844995582951943	9.889282559251389E-6	0.07333891945940829	9.88928255925139E-5
76-77	0.0	0.0014339459710914514	9.889282559251389E-6	0.07550467233988437	9.88928255925139E-5
78-79	0.0	0.0014636138187692055	9.889282559251389E-6	0.07748252885173464	9.88928255925139E-5
80-81	0.0	0.0014685584600488313	9.889282559251389E-6	0.07846156782510053	9.88928255925139E-5
82-83	0.0	0.001473503101328457	9.889282559251389E-6	0.07888680697514833	1.1867139071101667E-4
84-85	0.0	0.0014784477426080826	9.889282559251389E-6	0.07926259971239988	1.1867139071101667E-4
86-87	0.0	0.0015031709490062111	9.889282559251389E-6	0.07941093895078866	1.1867139071101667E-4
88-89	0.0	0.0015872298507598479	9.889282559251389E-6	0.07944060679846641	1.1867139071101667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16215	0.0	50.904305	1
GTATCAA	28970	0.0	38.780777	1
TCAACGC	33870	0.0	33.053005	4
ATCAACG	34180	0.0	32.650185	3
TATCAAC	34630	0.0	32.124054	2
CTTATAC	7860	0.0	31.975542	1
CAACGCA	35035	0.0	31.966497	5
AACGCAG	35985	0.0	31.168892	6
ACGCAGA	41360	0.0	27.065018	7
CGCAGAG	41900	0.0	26.71634	8
TATACAC	12290	0.0	24.647362	3
GCAGAGT	48525	0.0	23.068823	9
GTGGTAT	8330	0.0	22.813814	1
TGGTATC	7995	0.0	22.00493	2
GAGTACT	28960	0.0	21.822668	12-13
TTATACA	12440	0.0	21.620962	2
CAGAGTA	47195	0.0	20.43656	10-11
GTACTTT	31970	0.0	19.610147	14-15
ACATCTC	14105	0.0	18.934628	8
GTACATG	32545	0.0	18.710052	1
>>END_MODULE