FastQCFastQC Report
Fri 27 May 2016
SRR2139317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10006451
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148850.052.6607251
GTATCAA278500.040.3573341
TCAACGC324850.034.5989274
ATCAACG326850.034.2254373
CAACGCA336650.033.41245
TATCAAC337750.033.147022
AACGCAG347950.032.348356
TGGTATC66900.029.341492
GTGGTAT72500.029.082931
ACGCAGA402300.027.945277
CGCAGAG409900.027.437898
GCAGAGT475800.023.6839759
GAGTACT284550.021.90056812-13
CTTATAC64050.021.2119121
CAGAGTA466750.020.26101310-11
GTACTTT310450.019.98125514-15
GTACATG330950.019.419831
TACATGG324200.019.2390962
GTGTAGC63050.018.819821
ACATGGG327550.018.6921863