##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10006451
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.019230394472526	31.0	18.0	33.0	18.0	33.0
2	28.01408331485359	29.0	25.0	32.0	18.0	33.0
3	28.26017226287322	29.0	27.0	31.0	18.0	33.0
4	31.23549288354083	33.0	31.0	33.0	27.0	33.0
5	31.869513776662675	33.0	32.0	33.0	30.0	34.0
6	35.929343480520714	38.0	36.0	38.0	31.0	38.0
7	36.247949447811216	38.0	37.0	38.0	33.0	38.0
8	36.59332564562601	38.0	37.0	38.0	34.0	38.0
9	36.83716184689257	38.0	38.0	38.0	35.0	38.0
10-11	36.88019588563418	38.0	38.0	38.0	35.0	38.0
12-13	36.94354017223489	38.0	38.0	38.0	35.0	38.0
14-15	36.934727307413986	38.0	38.0	38.0	35.0	38.0
16-17	36.94860570446005	38.0	38.0	38.0	35.0	38.0
18-19	36.95810382722106	38.0	38.0	38.0	35.0	38.0
20-21	36.97208126866093	38.0	38.0	38.0	35.0	38.0
22-23	36.98470789060934	38.0	38.0	38.0	36.0	38.0
24-25	36.97048701780446	38.0	38.0	38.0	35.0	38.0
26-27	36.94116361646052	38.0	38.0	38.0	35.0	38.0
28-29	36.91931393296725	38.0	38.0	38.0	35.0	38.0
30-31	36.918886610953706	38.0	38.0	38.0	35.0	38.0
32-33	36.89614007903819	38.0	38.0	38.0	35.0	38.0
34-35	36.87060079198484	38.0	38.0	38.0	35.0	38.0
36-37	36.84265720653075	38.0	38.0	38.0	35.0	38.0
38-39	36.81940089944996	38.0	38.0	38.0	35.0	38.0
40-41	36.815890566082025	38.0	38.0	38.0	35.0	38.0
42-43	36.75860391416644	38.0	38.0	38.0	35.0	38.0
44-45	36.71779955794973	38.0	38.0	38.0	35.0	38.0
46-47	36.670990990058456	38.0	38.0	38.0	34.0	38.0
48-49	36.629905705225525	38.0	38.0	38.0	34.0	38.0
50-51	36.6005828374336	38.0	38.0	38.0	34.0	38.0
52-53	36.57724068279154	38.0	38.0	38.0	34.0	38.0
54-55	36.56335399376754	38.0	38.0	38.0	34.0	38.0
56-57	36.54191642706444	38.0	38.0	38.0	34.0	38.0
58-59	36.528240777915244	38.0	38.0	38.0	34.0	38.0
60-61	36.52795471362265	38.0	38.0	38.0	34.0	38.0
62-63	36.514793529796265	38.0	38.0	38.0	34.0	38.0
64-65	36.50407228485975	38.0	38.0	38.0	34.0	38.0
66-67	36.4773685712327	38.0	38.0	38.0	34.0	38.0
68-69	36.46054055666517	38.0	38.0	38.0	34.0	38.0
70-71	36.463758016792966	38.0	38.0	38.0	34.0	38.0
72-73	36.44663425598331	38.0	38.0	38.0	34.0	38.0
74-75	36.43875504636809	38.0	38.0	38.0	34.0	38.0
76-77	36.4264242919385	38.0	38.0	38.0	34.0	38.0
78-79	36.411496369853666	38.0	38.0	38.0	34.0	38.0
80-81	36.40686255020299	38.0	38.0	38.0	34.0	38.0
82-83	36.38640495087897	38.0	38.0	38.0	34.0	38.0
84-85	36.37887897884403	38.0	38.0	38.0	34.0	38.0
86-87	36.36218505347314	38.0	38.0	38.0	34.0	38.0
88-89	36.36028123405792	38.0	38.0	38.0	34.0	38.0
90-91	36.364550898897605	38.0	38.0	38.0	34.0	38.0
92-93	36.333107599636435	38.0	37.0	38.0	34.0	38.0
94-95	36.32459078684349	38.0	37.0	38.0	33.5	38.0
96-97	36.322701503742366	38.0	37.0	38.0	33.5	38.0
98-99	36.34953489169084	38.0	37.0	38.0	34.0	38.0
100-101	35.801385147139186	38.0	36.0	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	45.0
20	115.0
21	478.0
22	1939.0
23	5668.0
24	13068.0
25	24224.0
26	40606.0
27	62035.0
28	89556.0
29	121049.0
30	154013.0
31	196530.0
32	250432.0
33	330518.0
34	464796.0
35	756547.0
36	1822034.0
37	5672794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.279869955891456	18.80439928202317	13.817006649010724	22.098724113074656
2	14.598522493139676	20.12415790573501	41.156989625992274	24.120329975133043
3	19.761171417321417	25.697008096866586	29.42404515537149	25.1177753304405
4	12.833561069753902	18.271153279019707	39.29766907368057	29.59761657754583
5	13.762322800532957	38.14347570214392	34.52711780996246	13.56708368736066
6	29.810809047083726	37.81609483722051	18.71534672982459	13.657749385871174
7	26.625054177550062	33.24483375774288	22.409883384228834	17.720228680478222
8	24.20172746561193	36.979414579654666	20.77197999570477	18.04687795902863
9	25.39683650077335	17.760322815751557	21.207329151964068	35.63551153151102
10-11	24.149246321198195	27.095975386278308	28.422964345700592	20.331813946822905
12-13	25.122443511690605	24.415264712733816	28.78834363951815	21.67394813605743
14-15	22.268405218894596	25.850820098659376	26.64632211918145	25.234452563264583
16-17	21.58623471998214	29.138597690629776	27.27282130297745	22.00234628641064
18-19	21.529111570126112	28.044573445670196	29.330858663076448	21.09545632112724
20-21	22.44171793855768	27.065607200267433	29.35706972460836	21.135605136566532
22-23	22.03814825549437	27.065287785962	29.279705567826337	21.61685839071729
24-25	21.785646049801784	27.191145465250443	29.400191300442696	21.623017184505073
26-27	21.714631543295948	27.3745882855364	29.32132242065912	21.589457750508533
28-29	21.59486548030598	27.355855256536238	29.42788899107142	21.621390272086362
30-31	21.993627022704732	27.167437768897507	29.23555296686712	21.603382241530635
32-33	21.770480068283934	27.300498418187775	29.314337220476684	21.61468429305161
34-35	21.874907732104848	27.42135561648612	29.261847800303254	21.441888851105777
36-37	21.829285389695148	27.31419261894863	29.188582285089964	21.667939706266257
38-39	21.641440584715504	27.419301166576698	29.31380869687647	21.625449551831327
40-41	21.99353886124843	27.27854892584356	29.128760553698065	21.59915165920994
42-43	21.896596575096293	27.30753875299776	29.024193424014115	21.771671247891824
44-45	21.918163599852257	27.471297257616005	28.88748072913108	21.723058413400658
46-47	21.984322440046576	27.447834145195888	28.54015266843885	22.027690746318683
48-49	21.925429286434508	27.536226978535144	28.592121295786793	21.94622243924356
50-51	21.89123830538144	27.723199602162662	28.479701420816195	21.905860671639704
52-53	22.05013188303263	27.667832814352835	28.42013767250548	21.861897630109052
54-55	21.984263618549996	27.577928518004708	28.44505438225736	21.992753481187936
56-57	22.03141144633492	27.820023637228147	28.262450677433115	21.886114239003813
58-59	21.93359966102125	27.930569360750866	28.393729236065063	21.742101742162816
60-61	22.035649626331644	27.989154149469826	28.278929522616664	21.696266701581873
62-63	21.90887328868447	28.080807193446873	28.239347090665994	21.77097242720266
64-65	21.91813305989402	27.990218329405742	28.247666889471628	21.843981721228605
66-67	22.00294347292897	27.998687762068613	28.10605268372759	21.892316081274828
68-69	21.955717005700908	27.862695833900325	28.25309688889479	21.928490271503975
70-71	21.928168437671854	27.632028117458002	28.311745883936567	22.128057560933577
72-73	22.007212354200593	27.634047714440218	28.298607339414417	22.06013259194477
74-75	21.870360159006008	27.74214179015094	28.26086047652085	22.126637574322203
76-77	21.995819603093775	27.70565414862391	28.182684175171268	22.115842073111047
78-79	21.997202700112073	27.76044349443344	28.117068014114686	22.125285791339802
80-81	22.025515248138902	27.73049964192051	28.120217997154175	22.123767112786414
82-83	22.052533505789345	27.734727724838713	28.21450472265766	21.998234046714277
84-85	22.208466289724182	27.696347407545836	28.11953363953102	21.97565266319896
86-87	21.98755058100081	27.76236602443511	28.145686026044594	22.10439736851949
88-89	22.07350917400509	27.759772083566585	28.101533762485175	22.06518497994315
90-91	22.119686237608995	27.814602885931052	28.115958085081587	21.94975279137837
92-93	22.151341721939673	27.742360704590695	28.11692043819196	21.989377135277675
94-95	22.00018185622515	27.878345869439546	28.085852238218973	22.03562003611633
96-97	22.097019002647492	27.86261432602815	28.097686112411075	21.94268055891328
98-99	22.287091446193404	28.4319318218112	28.119954150416444	21.16102258157895
100-101	22.886069438793214	31.0140614348899	25.508587814832236	20.59128131148465
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2059.0
1	1912.0
2	2403.0
3	3347.5
4	4240.0
5	6079.5
6	8068.5
7	10422.0
8	14018.5
9	16441.0
10	15857.5
11	14641.0
12	15505.5
13	18920.0
14	25899.5
15	34346.0
16	41656.0
17	47183.5
18	48541.0
19	45530.0
20	40793.5
21	36223.5
22	34345.0
23	37183.5
24	44791.0
25	57361.0
26	74307.5
27	93538.0
28	114118.5
29	135834.5
30	157004.5
31	179281.5
32	204150.5
33	229022.0
34	252407.5
35	275116.5
36	299018.0
37	320089.0
38	335119.5
39	348033.0
40	364546.5
41	378675.0
42	385645.0
43	392409.5
44	400739.5
45	410612.0
46	415499.0
47	413584.5
48	409020.5
49	399857.5
50	390090.5
51	377460.5
52	357229.0
53	335563.0
54	314901.0
55	291642.5
56	266624.0
57	240085.5
58	212523.5
59	184330.0
60	153707.0
61	123683.0
62	96259.5
63	73228.5
64	54857.5
65	40316.0
66	29772.5
67	22765.5
68	17296.5
69	12687.5
70	9243.0
71	6592.5
72	4600.0
73	3065.0
74	1978.0
75	1230.0
76	776.5
77	474.5
78	289.0
79	178.0
80	120.0
81	79.5
82	58.0
83	46.0
84	34.0
85	23.0
86	18.5
87	16.0
88	11.0
89	7.5
90	5.5
91	3.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0011092844006331515
4	0.0
5	0.04865861033047581
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.4983882897143054E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.998354291849409E-6
28-29	4.999960500312048E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.0114504280053631E-5
40-41	0.0
42-43	0.0
44-45	5.1371857191785165E-6
46-47	3.051911093795108E-4
48-49	0.0
50-51	4.715200081394831E-5
52-53	1.580383604405119E-5
54-55	0.0019013117515206917
56-57	0.002572555656682381
58-59	9.05406649199283E-4
60-61	0.002808302571370234
62-63	0.0044302010438247745
64-65	0.0022583654027584677
66-67	0.007161542429283063
68-69	0.004187276465398798
70-71	0.0016719847858301742
72-73	7.578421505741412E-4
74-75	6.384154460831884E-4
76-77	0.0
78-79	0.0
80-81	1.788807397491815E-4
82-83	0.0
84-85	7.964582438903893E-4
86-87	8.61372584233389E-4
88-89	7.729602797117311E-5
90-91	3.1173751701067703E-4
92-93	1.5115382059722326E-4
94-95	1.2196928435315207E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	87.0
20-21	423.0
22-23	1057.0
24-25	1149.0
26-27	2457.0
28-29	5675.0
30-31	14283.0
32-33	18391.0
34-35	24811.0
36-37	39562.0
38-39	47540.0
40-41	47121.0
42-43	55060.0
44-45	65650.0
46-47	66138.0
48-49	59844.0
50-51	53457.0
52-53	49879.0
54-55	52708.0
56-57	54538.0
58-59	56018.0
60-61	56027.0
62-63	54377.0
64-65	61112.0
66-67	64252.0
68-69	63944.0
70-71	69644.0
72-73	57128.0
74-75	57840.0
76-77	61021.0
78-79	63884.0
80-81	65026.0
82-83	63195.0
84-85	62467.0
86-87	64654.0
88-89	68142.0
90-91	70974.0
92-93	70007.0
94-95	76775.0
96-97	136456.0
98-99	864309.0
100-101	7139369.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.28797523289808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71944828314466	73.89263211282935
2	7.760751175930161	12.927545034591118
3	1.9080523660463689	4.767534546190276
4	0.7212073527671059	2.402716005402028
5	0.3453258489429851	1.4380745377021424
6	0.17806624327264547	0.8898466115704379
7	0.11081510856013935	0.6460696212031458
8	0.06620503697582611	0.44112667839485653
9	0.042217259740723705	0.31645710753175993
>10	0.14293281724340096	1.8082611904361678
>50	0.004011015763220284	0.23000013295398405
>100	8.422487740235569E-4	0.11282480847512391
>500	8.349613529987776E-5	0.04914854350789935
>1k	4.174670341175148E-5	0.07776306921176357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.993553158857221E-6
2	0.0	3.9974212635428884E-5	0.0	0.0	9.993553158857221E-6
3	0.0	3.9974212635428884E-5	0.0	0.0	9.993553158857221E-6
4	0.0	8.994197842971499E-5	0.0	0.0	9.993553158857221E-6
5	0.0	9.993553158857222E-5	0.0	0.0	9.993553158857221E-6
6	8.994197842971499E-5	9.993553158857222E-5	0.0	0.0	9.993553158857221E-6
7	8.994197842971499E-5	9.993553158857222E-5	0.0	0.0	9.993553158857221E-6
8	8.994197842971499E-5	1.1992263790628665E-4	0.0	0.0	9.993553158857221E-6
9	8.994197842971499E-5	1.499032973828583E-4	0.0	0.0	3.9974212635428884E-5
10-11	8.994197842971499E-5	1.499032973828583E-4	0.0	0.0	3.9974212635428884E-5
12-13	8.994197842971499E-5	1.499032973828583E-4	0.0	4.9967765794286105E-6	3.9974212635428884E-5
14-15	9.993553158857222E-5	2.5983238213028774E-4	0.0	9.993553158857221E-6	6.495809553257194E-5
16-17	1.5989685054171554E-4	2.5983238213028774E-4	0.0	9.993553158857221E-6	9.49387550091436E-5
18-19	1.5989685054171554E-4	2.748227118685736E-4	0.0	9.993553158857221E-6	1.1992263790628665E-4
20-21	1.5989685054171554E-4	2.8981304160685944E-4	0.0	9.993553158857221E-6	1.2991619106514387E-4
22-23	1.7488718028000136E-4	3.297872542422883E-4	0.0	9.993553158857221E-6	1.3990974422400108E-4
24-25	1.7988395685942997E-4	3.497743605600027E-4	0.0	1.9987106317714442E-5	1.5490007396228692E-4
26-27	1.7988395685942997E-4	3.5976791371885995E-4	0.0	3.4977436056000276E-5	1.6489362712114415E-4
28-29	1.7988395685942997E-4	3.697614668777172E-4	0.0	2.1486139291543023E-4	1.6989040370057277E-4
30-31	1.848807334388586E-4	3.797550200365744E-4	0.0	6.845583913817196E-4	1.6989040370057277E-4
32-33	1.898775100182872E-4	3.8974857319543164E-4	0.0	0.0017338814730617279	1.6989040370057277E-4
34-35	1.898775100182872E-4	4.5470666872800356E-4	0.0	0.0032029337874137395	1.9487428659771582E-4
36-37	1.898775100182872E-4	5.296583174194328E-4	0.0	0.005271599291297184	1.9987106317714444E-4
38-39	1.898775100182872E-4	5.296583174194328E-4	0.0	0.008344616887645779	2.1486139291543026E-4
40-41	1.898775100182872E-4	5.796260832137189E-4	0.0	0.011777402397713235	2.2985172265371608E-4
42-43	1.898775100182872E-4	5.996131895314332E-4	0.0	0.015270149226733833	2.2985172265371608E-4
44-45	1.998710631771444E-4	6.845583913817196E-4	0.0	0.019242586607379576	2.2985172265371608E-4
46-47	2.0986461633600164E-4	7.645068166525775E-4	0.0	0.023314959519613897	2.2985172265371608E-4
48-49	2.1985816949485887E-4	7.745003698114346E-4	0.0	0.027422309867904216	2.3984527581257328E-4
50-51	2.448420523920019E-4	8.444552419234352E-4	0.0	0.0314896860035591	2.498388289714305E-4
52-53	2.5983238213028774E-4	8.59445571661721E-4	0.0	0.03543214272472828	2.5983238213028774E-4
54-55	2.5983238213028774E-4	8.59445571661721E-4	0.0	0.039739364136195734	2.848162650274308E-4
56-57	2.5983238213028774E-4	8.694391248205782E-4	0.0	0.04383172415474777	2.998065947657166E-4
58-59	2.5983238213028774E-4	8.744359014000068E-4	0.0	0.04793907450303809	2.998065947657166E-4
60-61	2.798194884480022E-4	8.994197842971498E-4	0.0	0.052281273350561555	2.998065947657166E-4
62-63	3.0980014792457384E-4	9.343972203531502E-4	0.0	0.05620873974199244	3.0980014792457384E-4
64-65	3.0980014792457384E-4	0.0010093488690445793	0.0	0.06020616100553533	3.0980014792457384E-4
66-67	3.347840308217169E-4	0.0010093488690445793	0.0	0.06403868864195708	3.4977436056000277E-4
68-69	3.3978080740114554E-4	0.0010193424222034366	0.0	0.06804610345865882	3.4977436056000277E-4
70-71	3.447775839805742E-4	0.0010293359753622937	0.0	0.07195857952035142	3.5976791371885995E-4
72-73	3.797550200365744E-4	0.0010293359753622937	0.0	0.07495164869142916	3.9974212635428887E-4
74-75	3.8974857319543164E-4	0.001039329528521151	0.0	0.07731012723691946	3.9974212635428887E-4
76-77	3.9974212635428887E-4	0.001064313411418294	0.0	0.07956367347424177	3.9974212635428887E-4
78-79	3.9974212635428887E-4	0.0010892972943154372	0.0	0.08161734864838693	3.9974212635428887E-4
80-81	3.9974212635428887E-4	0.0010892972943154372	0.0	0.08228191993345094	4.247260092514319E-4
82-83	3.9974212635428887E-4	0.0010892972943154372	0.0	0.0826966523895435	4.5970344530743215E-4
84-85	3.9974212635428887E-4	0.0010992908474742942	0.0	0.08303143642036523	4.5970344530743215E-4
86-87	3.9974212635428887E-4	0.0011192779537920088	0.0	0.08318133971774808	4.74693775045718E-4
88-89	3.9974212635428887E-4	0.0011892328259040094	0.0	0.08327627847275722	4.796905516251466E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14885	0.0	52.660725	1
GTATCAA	27850	0.0	40.357334	1
TCAACGC	32485	0.0	34.598927	4
ATCAACG	32685	0.0	34.225437	3
CAACGCA	33665	0.0	33.4124	5
TATCAAC	33775	0.0	33.14702	2
AACGCAG	34795	0.0	32.34835	6
TGGTATC	6690	0.0	29.34149	2
GTGGTAT	7250	0.0	29.08293	1
ACGCAGA	40230	0.0	27.94527	7
CGCAGAG	40990	0.0	27.43789	8
GCAGAGT	47580	0.0	23.683975	9
GAGTACT	28455	0.0	21.900568	12-13
CTTATAC	6405	0.0	21.211912	1
CAGAGTA	46675	0.0	20.261013	10-11
GTACTTT	31045	0.0	19.981255	14-15
GTACATG	33095	0.0	19.41983	1
TACATGG	32420	0.0	19.239096	2
GTGTAGC	6305	0.0	18.81982	1
ACATGGG	32755	0.0	18.692186	3
>>END_MODULE