FastQCFastQC Report
Fri 27 May 2016
SRR2139316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10568833
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160650.050.8599971
GTATCAA290850.038.6837961
TCAACGC337050.033.1590654
ATCAACG338900.032.900033
CAACGCA347300.032.155055
TATCAAC346600.032.0038342
AACGCAG358700.031.1672126
TGGTATC73950.029.141912
GTGGTAT79300.029.0097941
ACGCAGA419650.026.6089947
CGCAGAG427600.026.145188
GCAGAGT501150.022.3607869
GAGTACT300550.021.14154812-13
CAGAGTA490950.019.60881210-11
GTACATG345450.019.4677931
TACATGG334400.019.4126052
CTTATAC64850.019.2999291
GTACTTT330550.019.13648614-15
ACATGGG336700.018.8742393
AGAGTAC456300.017.86390310-11
Produced by FastQC (version 0.11.5)