FastQCFastQC Report
Fri 27 May 2016
SRR2139315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10812726
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155350.051.6368941
GTATCAA284850.037.826981
TCAACGC325650.032.9250834
ATCAACG327900.032.5651133
CAACGCA335650.031.9441495
TATCAAC334400.031.9189472
AACGCAG346150.031.0255936
ACGCAGA404900.026.5238537
GTGGTAT74550.026.3541281
CGCAGAG409500.026.2475348
TGGTATC74250.024.8587322
GCAGAGT484750.022.1730069
CTTATAC71750.022.1025311
GAGTACT280600.021.01985212-13
CAGAGTA469850.019.83506610-11
TATACAC107950.019.5467383
GTACATG342200.019.258081
TACATGG337500.018.8475482
GTACTTT313300.018.6505314-15
AGAGTAC435600.017.95269810-11
Produced by FastQC (version 0.11.5)