##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10812726
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.316810580421624	31.0	18.0	33.0	18.0	33.0
2	28.354073154170372	30.0	27.0	33.0	18.0	33.0
3	28.569067134411803	31.0	27.0	33.0	18.0	33.0
4	31.330397071006885	33.0	31.0	33.0	28.0	33.0
5	31.916160180143287	33.0	32.0	33.0	30.0	34.0
6	35.94586970945162	38.0	36.0	38.0	31.0	38.0
7	36.24737008965177	38.0	37.0	38.0	33.0	38.0
8	36.60136167327277	38.0	37.0	38.0	34.0	38.0
9	36.8396656865253	38.0	38.0	38.0	35.0	38.0
10-11	36.89097194361533	38.0	38.0	38.0	35.0	38.0
12-13	36.95354714435564	38.0	38.0	38.0	35.0	38.0
14-15	36.94951324023193	38.0	38.0	38.0	35.0	38.0
16-17	36.964045560758684	38.0	38.0	38.0	35.0	38.0
18-19	36.97581359224307	38.0	38.0	38.0	35.0	38.0
20-21	36.9866506510043	38.0	38.0	38.0	35.5	38.0
22-23	37.001858715601	38.0	38.0	38.0	36.0	38.0
24-25	36.99414484662896	38.0	38.0	38.0	36.0	38.0
26-27	36.96378841909191	38.0	38.0	38.0	35.5	38.0
28-29	36.94066494833866	38.0	38.0	38.0	35.0	38.0
30-31	36.93812614905938	38.0	38.0	38.0	35.0	38.0
32-33	36.91301924985437	38.0	38.0	38.0	35.0	38.0
34-35	36.890912001748674	38.0	38.0	38.0	35.0	38.0
36-37	36.86307624817276	38.0	38.0	38.0	35.0	38.0
38-39	36.838914402734275	38.0	38.0	38.0	35.0	38.0
40-41	36.84095767816342	38.0	38.0	38.0	35.0	38.0
42-43	36.78420041047069	38.0	38.0	38.0	35.0	38.0
44-45	36.74762146857503	38.0	38.0	38.0	35.0	38.0
46-47	36.7061929883035	38.0	38.0	38.0	35.0	38.0
48-49	36.67066980487982	38.0	38.0	38.0	34.0	38.0
50-51	36.642694105208676	38.0	38.0	38.0	34.0	38.0
52-53	36.620151053080534	38.0	38.0	38.0	34.0	38.0
54-55	36.60504155747716	38.0	38.0	38.0	34.0	38.0
56-57	36.58534417406253	38.0	38.0	38.0	34.0	38.0
58-59	36.57218690241041	38.0	38.0	38.0	34.0	38.0
60-61	36.570647483254504	38.0	38.0	38.0	34.0	38.0
62-63	36.559256289025306	38.0	38.0	38.0	34.0	38.0
64-65	36.55170499070331	38.0	38.0	38.0	34.0	38.0
66-67	36.52212687742756	38.0	38.0	38.0	34.0	38.0
68-69	36.50708322037016	38.0	38.0	38.0	34.0	38.0
70-71	36.51200574658668	38.0	38.0	38.0	34.0	38.0
72-73	36.49611675186969	38.0	38.0	38.0	34.0	38.0
74-75	36.48975135627364	38.0	38.0	38.0	34.0	38.0
76-77	36.47508653023833	38.0	38.0	38.0	34.0	38.0
78-79	36.459726773631814	38.0	38.0	38.0	34.0	38.0
80-81	36.458188685559534	38.0	38.0	38.0	34.0	38.0
82-83	36.436738532613006	38.0	38.0	38.0	34.0	38.0
84-85	36.42819147145801	38.0	38.0	38.0	34.0	38.0
86-87	36.41232298457231	38.0	38.0	38.0	34.0	38.0
88-89	36.41343257550621	38.0	38.0	38.0	34.0	38.0
90-91	36.414480316013645	38.0	38.0	38.0	34.0	38.0
92-93	36.384624601143	38.0	38.0	38.0	34.0	38.0
94-95	36.37726550350969	38.0	38.0	38.0	34.0	38.0
96-97	36.373748741510504	38.0	38.0	38.0	34.0	38.0
98-99	36.401279486726025	38.0	38.0	38.0	34.0	38.0
100-101	35.85877316114996	38.0	36.5	38.0	31.5	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	29.0
20	110.0
21	508.0
22	1952.0
23	5662.0
24	13154.0
25	25174.0
26	42323.0
27	64896.0
28	93604.0
29	126254.0
30	163273.0
31	208258.0
32	266060.0
33	349351.0
34	491853.0
35	796138.0
36	1890333.0
37	6273789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.85435957592932	18.968639360694056	13.74815194614198	22.428849117234638
2	14.596171215288356	20.295742257780322	40.99189233131405	24.116194195617275
3	19.645156543768987	25.64810736068174	29.511646552322894	25.19508954322638
4	12.868993443466522	18.25418492986875	39.17469100761455	29.70213061905018
5	13.836302297693424	38.00578494655494	34.512748719391226	13.645164036360406
6	29.886996119202504	37.70732745840411	18.747020871517506	13.658655550875883
7	26.604567617823665	33.10786752572848	22.50773764173808	17.77982721470978
8	24.200317292789997	36.87417955472098	20.898245271358952	18.02725788113007
9	25.372019969802253	17.66692321621763	21.258015786213395	35.70304102776672
10-11	24.182014785170733	26.9728651220793	28.478100712068354	20.367019380681615
12-13	25.085299959075996	24.38195736967323	28.75136471148577	21.781377959765
14-15	22.280325078196086	25.784109948637816	26.518263695222338	25.41730127794376
16-17	21.72092865388432	29.034301803264047	27.194127549333995	22.05064199351764
18-19	21.680541058748737	27.997948898362914	29.101676117567393	21.21983392532096
20-21	22.495556094023986	27.06362785501306	29.133470704440022	21.307345346522933
22-23	22.143849622277116	27.06665456902762	29.00164326263706	21.7878525460582
24-25	21.985869986131384	27.142074944174276	29.147203839239122	21.724851230455215
26-27	21.889274870187506	27.328621535795232	29.05196602610094	21.730137567916323
28-29	21.72197884828379	27.307615481238397	29.163710574428354	21.80669509604946
30-31	22.125880748624002	27.105305308670495	29.11455199709827	21.65426194560723
32-33	21.83623084264312	27.348583499496943	29.029923919220792	21.785261738639154
34-35	21.971582583527397	27.374920937512638	29.070550460804757	21.582946018155212
36-37	22.048790337541327	27.255211641755483	28.947397614365638	21.748600406337555
38-39	21.769906168632627	27.361121117274905	29.060165389365228	21.808807324727237
40-41	22.103796730403417	27.186731394493247	28.936202118121496	21.773269756981843
42-43	22.030825065521984	27.239621849144672	28.822478110334842	21.9070749749985
44-45	22.019825794416413	27.409615352428133	28.667138609763672	21.903420243391782
46-47	22.177070881842976	27.340985520769024	28.341543117718647	22.140400479669353
48-49	22.03329601469531	27.460299418683405	28.40211059023123	22.104293976390057
50-51	22.0237676651467	27.62078335056802	28.310933740926085	22.0445152433592
52-53	22.174639743283823	27.592492862993918	28.243656429435553	21.98921096428671
54-55	22.12446263748453	27.511401407310416	28.205057918393962	22.159078036811092
56-57	22.181939045250722	27.71189894080682	28.052082058503725	22.05407995543874
58-59	22.05710099764083	27.827332673748394	28.207286353912238	21.90827997469854
60-61	22.19371007163376	27.83063033384388	28.12593661365851	21.849722980863852
62-63	22.033121927644387	27.95911135938684	28.049980142241154	21.95778657072762
64-65	22.04287966077663	27.88330863335531	28.03311651155269	22.040695194315372
66-67	22.14393689339323	27.865697423186948	27.948500606014807	22.041865077405014
68-69	22.0882845028599	27.799472914866147	28.03616672370873	22.07607585856522
70-71	22.092097493606946	27.535543800986666	28.11920503984681	22.253153665559577
72-73	22.181606500168424	27.506493819015628	28.093795805651034	22.218103875164914
74-75	22.046326805051127	27.62284422155503	28.07156629433431	22.259262679059532
76-77	22.193690338491844	27.57642786893174	27.99251280476589	22.237368987810527
78-79	22.1566965128617	27.648834296442654	27.9179765928534	22.276492597842246
80-81	22.196777697369292	27.62505792814183	27.911650492877577	22.266513881611306
82-83	22.219089867263758	27.625637137200385	27.972059393770504	22.183213601765353
84-85	22.3241841646488	27.58199381092956	27.937540786122188	22.156281238299453
86-87	22.150720699381065	27.627734424690644	27.98666947401666	22.23487540191163
88-89	22.245563176512558	27.63944993349643	27.882679100566154	22.232307789424855
90-91	22.2810192355282	27.670025477487563	27.883764098790948	22.16519118819329
92-93	22.27595341243923	27.63355551977517	27.920350988703703	22.1701400790819
94-95	22.16071399897972	27.753363303248996	27.881377824800534	22.204544872970754
96-97	22.245627361513765	27.740892608482703	27.90003838167624	22.11344164832729
98-99	22.48161298009765	28.30286800094943	27.92462882787757	21.290890191075345
100-101	23.108748620028837	30.864262038882202	25.274343321464716	20.752646019624244
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2196.0
1	2028.0
2	2543.5
3	3566.0
4	4585.0
5	6415.0
6	8363.5
7	10543.5
8	13720.0
9	15928.5
10	15467.5
11	14541.5
12	15388.5
13	18674.5
14	25451.0
15	33624.0
16	41076.0
17	46718.0
18	47894.0
19	44743.5
20	40437.5
21	36674.0
22	35405.0
23	38694.5
24	46355.0
25	59089.5
26	76318.5
27	96439.5
28	118724.0
29	142663.5
30	166089.0
31	190398.5
32	216105.0
33	241835.0
34	267154.0
35	290998.0
36	317457.5
37	342174.5
38	360367.5
39	374916.5
40	391817.5
41	408836.5
42	419819.5
43	429692.5
44	447332.5
45	459130.5
46	457123.0
47	453923.5
48	447118.0
49	436135.5
50	424099.5
51	408654.0
52	388311.5
53	366920.0
54	345703.5
55	321527.0
56	294660.5
57	266202.0
58	237599.0
59	206480.0
60	172285.5
61	139917.0
62	109339.0
63	82881.0
64	61969.0
65	45574.0
66	33807.5
67	25809.0
68	19637.5
69	14318.5
70	10352.5
71	7383.0
72	5181.0
73	3534.5
74	2332.0
75	1464.5
76	865.5
77	497.5
78	320.5
79	216.5
80	151.0
81	104.5
82	67.0
83	44.0
84	33.0
85	25.0
86	18.5
87	12.0
88	11.5
89	12.0
90	8.0
91	4.5
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0011745419240254493
4	0.0
5	0.04962670838047686
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.248361606499601E-6
14-15	4.16176272292482E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	9.251092172378006E-6
28-29	9.254054282153987E-6
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	1.8724981376835708E-5
40-41	0.0
42-43	0.0
44-45	1.901973430572359E-5
46-47	2.489693387556302E-4
48-49	0.0
50-51	4.364846246108557E-5
52-53	2.9260509387429773E-5
54-55	0.0016721424838004454
56-57	0.0021358720449864968
58-59	7.791875723850366E-4
60-61	0.0026320008350484625
62-63	0.004442231815455218
64-65	0.0021439674116953424
66-67	0.00715393574156705
68-69	0.004088189794185548
70-71	0.0016751091651581638
72-73	6.510835123465748E-4
74-75	6.973547499191488E-4
76-77	0.0
78-79	0.0
80-81	1.2326546729724276E-4
82-83	0.0
84-85	7.50897036291017E-4
86-87	8.937382014905909E-4
88-89	8.29151966032629E-5
90-91	3.679368049583164E-4
92-93	2.1370704621937893E-4
94-95	1.1347797803803953E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	97.0
20-21	397.0
22-23	918.0
24-25	1283.0
26-27	2699.0
28-29	5917.0
30-31	15745.0
32-33	20510.0
34-35	28056.0
36-37	43279.0
38-39	51459.0
40-41	51198.0
42-43	58811.0
44-45	70789.0
46-47	71787.0
48-49	65311.0
50-51	57899.0
52-53	55555.0
54-55	59264.0
56-57	61611.0
58-59	62327.0
60-61	62530.0
62-63	61212.0
64-65	67394.0
66-67	71823.0
68-69	71694.0
70-71	78039.0
72-73	63140.0
74-75	64883.0
76-77	68389.0
78-79	71129.0
80-81	71868.0
82-83	69519.0
84-85	69306.0
86-87	72795.0
88-89	75717.0
90-91	78642.0
92-93	77037.0
94-95	85418.0
96-97	150895.0
98-99	933017.0
100-101	7663367.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.26942246865725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.6061147407985	73.78180001657869
2	7.824896857929578	13.031492844732135
3	1.942244254054332	4.851886719845167
4	0.7290900909273421	2.4284364319656233
5	0.35123261678983037	1.462346857612219
6	0.18030684604176758	0.9008428162225851
7	0.10577110469931136	0.6165249161528474
8	0.06571675560403216	0.43777570285293227
9	0.046034358280844005	0.3449928984985017
>10	0.144642550199021	1.8354870950013555
>50	0.003289064366034541	0.18072831460383243
>100	6.026514238734709E-4	0.0848309646908974
>500	3.873963788721321E-5	0.023274227166812104
>1k	1.936924769119126E-5	0.01958019407642013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	9.248361606499601E-6
3	0.0	0.0	0.0	0.0	9.248361606499601E-6
4	0.0	3.6993446425998405E-5	0.0	9.248361606499601E-6	9.248361606499601E-6
5	0.0	3.6993446425998405E-5	0.0	9.248361606499601E-6	9.248361606499601E-6
6	1.3872542409749402E-4	4.6241808032498E-5	0.0	9.248361606499601E-6	9.248361606499601E-6
7	1.3872542409749402E-4	4.6241808032498E-5	0.0	1.8496723212999203E-5	9.248361606499601E-6
8	1.3872542409749402E-4	6.473853124549721E-5	0.0	1.8496723212999203E-5	9.248361606499601E-6
9	1.3872542409749402E-4	1.3872542409749402E-4	0.0	1.8496723212999203E-5	9.248361606499601E-6
10-11	1.525979665072434E-4	1.3872542409749402E-4	0.0	1.8496723212999203E-5	1.8496723212999203E-5
12-13	1.572221473104932E-4	1.3872542409749402E-4	0.0	1.8496723212999203E-5	1.8496723212999203E-5
14-15	1.942155937364916E-4	2.1271231694949081E-4	0.0	2.7745084819498802E-5	2.3120904016249E-5
16-17	3.144442946209864E-4	2.1271231694949081E-4	0.0	2.7745084819498802E-5	2.7745084819498802E-5
18-19	3.144442946209864E-4	2.2196067855599042E-4	0.0	2.7745084819498802E-5	3.6993446425998405E-5
20-21	3.144442946209864E-4	2.3120904016249002E-4	0.0	2.7745084819498802E-5	3.6993446425998405E-5
22-23	3.144442946209864E-4	2.4970576337548925E-4	0.0	3.2369265622748604E-5	3.6993446425998405E-5
24-25	3.144442946209864E-4	2.682024865884884E-4	0.0	5.5490169638997604E-5	3.6993446425998405E-5
26-27	3.144442946209864E-4	2.7745084819498804E-4	0.0	7.861107365524661E-5	3.6993446425998405E-5
28-29	3.144442946209864E-4	2.7745084819498804E-4	0.0	3.00571752211237E-4	3.6993446425998405E-5
30-31	3.144442946209864E-4	3.283168370307358E-4	0.0	8.462250869947134E-4	4.16176272292482E-5
32-33	3.144442946209864E-4	3.329410178339856E-4	0.0	0.002298217859215151	5.5490169638997604E-5
34-35	3.144442946209864E-4	3.838070066697334E-4	0.0	0.003986043852401328	6.473853124549721E-5
36-37	3.144442946209864E-4	4.346729955054812E-4	0.0	0.006792921599973956	6.473853124549721E-5
38-39	3.144442946209864E-4	4.4392135711198084E-4	0.0	0.010815958898801282	7.398689285199681E-5
40-41	3.144442946209864E-4	4.716664419314796E-4	0.0	0.014917607271283856	7.398689285199681E-5
42-43	3.375651986372354E-4	4.716664419314796E-4	0.0	0.019130235983044423	8.32352544584964E-5
44-45	3.5143774104698486E-4	5.271566115704772E-4	0.0	0.02388389384878522	9.2483616064996E-5
46-47	3.653102834567342E-4	5.826467812094749E-4	0.0	0.029099969794850994	1.0635615847474541E-4
48-49	3.69934464259984E-4	5.872709620127247E-4	0.0	0.03408946088155753	1.2947706249099442E-4
50-51	4.1617627229248205E-4	7.39868928519968E-4	0.0	0.03911132123388681	1.3872542409749402E-4
52-53	4.2542463389898163E-4	7.583656517329673E-4	0.0	0.043888100003643855	1.3872542409749402E-4
54-55	4.346729955054812E-4	7.583656517329673E-4	0.0	0.04903018905685763	1.4334960490074383E-4
56-57	4.531697187184804E-4	7.676140133394668E-4	0.0	0.05418152647167791	1.4797378570399362E-4
58-59	4.716664419314796E-4	7.722381941427166E-4	0.0	0.059161769196777944	1.4797378570399362E-4
60-61	4.947873459477286E-4	7.907349173557159E-4	0.0	0.063809070904044	1.664705089169928E-4
62-63	5.456533347834764E-4	8.184800021752147E-4	0.0	0.06788759837251032	1.757188705234924E-4
64-65	5.456533347834764E-4	9.294603414532099E-4	0.0	0.0719430049369604	1.757188705234924E-4
66-67	5.549016963899761E-4	9.340845222564596E-4	0.0	0.07600765986301697	1.84967232129992E-4
68-69	5.549016963899761E-4	9.47957064666209E-4	0.0	0.08035901399887503	1.988397745397414E-4
70-71	5.641500579964756E-4	9.664537878792083E-4	0.0	0.08439129965930886	2.034639553429912E-4
72-73	5.733984196029752E-4	9.94198872698707E-4	0.0	0.08773458238005846	2.1271231694949081E-4
74-75	5.826467812094749E-4	0.0010265681383214557	0.0	0.09057382939325384	2.2196067855599042E-4
76-77	5.918951428159745E-4	0.001063561584747454	0.0	0.09325585425913872	2.2196067855599042E-4
78-79	5.918951428159745E-4	0.001063561584747454	0.0	0.09541997087505963	2.2196067855599042E-4
80-81	5.918951428159745E-4	0.001063561584747454	0.0	0.09649740500221683	2.2658485935924023E-4
82-83	5.965193236192243E-4	0.001063561584747454	0.0	0.09684421856246056	2.3120904016249002E-4
84-85	6.196402276354733E-4	0.0010728099463539536	0.0	0.09718178376109779	2.3120904016249002E-4
86-87	6.335127700452226E-4	0.0010820583079604533	0.0	0.0973482542700148	2.4508158257223944E-4
88-89	6.47385312454972E-4	0.0011652935624189497	0.0	0.09738524771644079	2.497057633754892E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15535	0.0	51.636894	1
GTATCAA	28485	0.0	37.82698	1
TCAACGC	32565	0.0	32.925083	4
ATCAACG	32790	0.0	32.565113	3
CAACGCA	33565	0.0	31.944149	5
TATCAAC	33440	0.0	31.918947	2
AACGCAG	34615	0.0	31.025593	6
ACGCAGA	40490	0.0	26.523853	7
GTGGTAT	7455	0.0	26.354128	1
CGCAGAG	40950	0.0	26.247534	8
TGGTATC	7425	0.0	24.858732	2
GCAGAGT	48475	0.0	22.173006	9
CTTATAC	7175	0.0	22.102531	1
GAGTACT	28060	0.0	21.019852	12-13
CAGAGTA	46985	0.0	19.835066	10-11
TATACAC	10795	0.0	19.546738	3
GTACATG	34220	0.0	19.25808	1
TACATGG	33750	0.0	18.847548	2
GTACTTT	31330	0.0	18.65053	14-15
AGAGTAC	43560	0.0	17.952698	10-11
>>END_MODULE